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Antibacterial Activity of Litsea Glutinosa Bark
Antibacterial Activity of Litsea Glutinosa Bark
439441
Short report
Abstract
The methanol extract of the bark of Litsea glutinosa showed antibacterial activity,
comparable to chloramphenicol, against all 16 tested microorganisms. 2000 Elsevier
Science B.V. All rights reserved.
Keywords: Litsea glutinosa; Antibacterial activity
Plant. Litsea glutinosa Lour. Lauraceae ., bark collected from Hetyasole, Bankura,
West Bengal, India, in August 1997 and identified Ref. No. BSIrCNHrTech.II.
by Dr G.S. Giri of Botanical Survey of India, Shibpur, Howrah. A voucher
specimen has been deposited in the Pharmacognosy Division of the Department of
Pharmaceutical Technology, Jadavpur University, Calcutta.
Uses in traditional medicine. The bark of L. glutinosa is widely used as a
demulcent. Ground and pasted material is used as an emollient. Mucilaginous bark
U
0367-326Xr00r$ - see front matter 2000 Elsevier Science B.V. All rights reserved.
PII: S 0 3 6 7 - 3 2 6 X 0 0 . 0 0 1 3 2 - 5
440
Table 1
Antibacterial activity of Litsea glutinosa bark methanol extract a
Microorganisms
Extract grml.
50
Staphylococcus aureus
ML 267
100
Chloramphenicol grml.
150
200
50
100
150
200
6.50
8.00
8.50
9.00
8.50
9.00
9.50
10.00
7.00
8.00
9.00
10.50
9.00
9.50
10.00
12.00
Bacillus subtilis
CDr99r1
7.00
8.00
9.50
10.00
9.00
9.50
9.50
11.00
Lactobacillus arabinosus
CDr99r1
7.00
8.00
9.00
10.00
8.50
9.50
10.00
11.00
Bacillus cereus
V. mycoides
7.00
8.50
10.00
13.50
6.50
8.00
8.50
9.50
Klebsiella pneumoniae
RM8r98
7.00
8.00
8.50
9.50
7.00
8.00
8.50
9.00
Streptococcus pneumoniae
7465
7.00
7.50
8.50
9.00
7.00
8.00
9.00
10.00
Sarcina lutea
CDr99r1
7.00
8.00
8.50
9.00
6.50
8.00
8.50
8.75
Shigella dysenteriae
1
7.50
8.50
9.50
10.00
7.00
8.00
9.00
10.00
Shigella sonnei
2
7.00
9.00
11.00
12.00
7.00
9.50
13.00
16.00
Escherichia coli
Row 7r12
7.50
8.00
9.50
10.00
8.00
8.50
9.50
10.50
E. coli
AM8r98
8.00
8.50
9.50
11.00
8.00
9.00
9.50
10.50
E. coli
VC Sona wave 3: 37C
9.00
10.50
11.50
12.50
8.50
9.00
9.50
10.00
Salmonella typhimurium
2
8.00
8.50
9.00
9.50
8.00
8.50
9.00
9.50
Proteus mirabilis
AM8r98
7.50
9.00
9.50
10.50
7.00
8.00
8.50
9.00
Vibrio cholerae
865
9.50
10.00
10.50
11.00
7.50
8.00
9.00
10.00
Bacillus pumilus
8241
441
is used in diarrhoea and dysentery treatment, and also for sprains, bruises and
rheumatic gouty joints w1,2x.
Previously isolated constituents. No report.
Tested material. Methanol extract yield: 7.5%, on air-dried bark.. Phytochemical
screening w35x gave positive tests for alkaloids, steroids, triterpenoids, saponins,
and tannins.
Studied activity. Antibacterial activity by an agar diffusion method w6x.
Used microorganisms. Staphylococcus aureus ML 267., Bacillus pumilus 8241.,
Streptococcus pneumoniae 7465., Escherichia coli Row 7r12. obtained from the
Division of Microbiology, Department of Pharmaceutical Technology, Jadavpur,
Calcutta, India. Bacillus subtilis CDr99r1., Lactobacillus arabinosus CDr99r1.,
Bacillus cereus V. mycoides., Sarcina lutea CDr99r1., Shigella dysenteriae 1.,
Shigella sonnei 2., Salmonella typhimurium 2., Vibrio cholerae 865. obtained from
the Central Drug Laboratory, Calcutta, India. Klebsiella pneumoniae RM8r98., E.
coli AM8r98., E. coli VC Sona wave 3: 37C., Proteus mirabilis AM8r98.
obtained from Institute of Microbial Technology, Chandigarh, India.
Results. Reported in Table 1.
Conclusions. The methanol extract of Litsea glutinosa bark effectively inhibited
both gram-positive and gram-negative bacteria. The results justify the reported
uses in diarrhoea and dysentery in traditional medicine.
Acknowledgements
The authors are grateful to Mr Manoranjan Mandal of Hetyasole, Bankura,
West Bengal, India for collection of plant material.
References
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