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Tejassdfsd
Tejassdfsd
3(4), 2011,1146-1155
INTRODUCTION: [1,2,5]
Biological Name: The dried ripe flowers,
leaves of Hibiscus rosa-sinenis belongs to
family Malvaceae.[1]
Source: Widely spread all over the world,
majorly in tropical and subtropical areas.[2]
Description: The leaves are alternate, simple
and ovate to lanceolate, often with a toothed or
lobed margin. The flowers are large,
conspicuous, trumpet-shaped, with five or
more petals, ranging from white to pink, red,
orange, purple or yellow, and from 418 cm
broad. [2, 6]
Synonyms: Aloala [Hawai]; red hibiscus;
China rose [English]; da hong hua (big red
flower) [China]; Jasud [Gujarati], japapushpam
[India]; shoeflower [Jamaica]. [5]
Nomenclature: Hibiscus is taken from the
Greek "hibiscos," a name for mallow. [6]
Pharmacological Actions: Abortifacient[5];
analgesic[5]; antidiarrhoic[5]; antiestrogenic[1];
antifungal[5]; anti-infectious; anti-inflammatory;
antipyretic; astringent[5]; CNS depressant[1];
constipating[1]; contraceptive; demulcent[5];
diuretic[6];
expectorant[5];
dentifrice[6];
hypotensive;
hemostat;
hypoglycemic[5];
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Result:
Control
Activity and
Model
Parameters
Standard
Test
Before
Reading
SEM
After
Reading
SEM
Before
Reading
SEM
After
Reading
SEM
Before
Reading
SEM
After
Reading
SEM
Onset of action
(Minutes)
---
---
---
26.50.84
---
33.161.25
Duration of action
(Minutes)
---
---
---
187.664.75
---
1593.41
Number of crossing
18.751.1
21.51.5
191.04
48.51.73
161.42
35.830.97
8.160.49
8.50.27
7.50.52
18.332.12
80.44
140.89
Number of Entry in
light area
10.50.64
110.70
90.40
20.50.6
10.660.4
180.40
250.70
240.70
240.70
1120.9
220.70
72.50.93
CNS-depressant
(Actophotometer)
Number of cut-off
52.50.76
530.63
51.50.8
9.50.62
510.96
200.36
SKM-relaxant
(Rota-rod model)
Time of fall
(Seconds)
---
246.63.3
---
5.830.60
---
17.50.80
Sedative
(Phenobarbital
induce sleep)
Anxiolytic
(open field model)
Anxiolytic
(Elevated plus
model)
Anxiolytic
(Light/Dark
model)
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ACTOPHOTOMETER
ROTAROD
Table 1: Sedative effect of MEHR on rat using Phenobarbital induced sleep model
For Sedative: phenobarbiatal induced sleep model
Control (Seconds)
Sr. No.
Group
Onset of
Duration
time
of action
1
Head
----2
Tail
----3
Back
----4
Head back
----5
Back tail
----6
No mark
----Average
----SD
----SEM
----variance
-----
Standard (Seconds)
Onset of
Duration of
time
action
26
190
27
180
24
189
30
200
27
169
25
198
26.5
187.66
2.073
11.60
0.84
4.75
6.25
66.91
Test (Seconds)
Onset of
Duration of
time
action
30
155
34
154
29
148
35
167
34
160
37
170
33.16
159
3.06
8.34
1.25
3.41
8.66
63.33
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Test
Before
20
18
12
16
15
14
15.833
2.8577
1.4289
8.16
After
39
36
33
36
36
35
35.833
1.9408
0.9704
3.76
Head
Tail
Back
head back
back tail
No mark
Test
Before
After
Before
After
Before
After
9
8
9
7
9
7
8.16
0.98
0.49
0.96
8
9
9
8
9
8
8.5
0.54
0.27
0.3
7
6
9
8
7
8
7.5
1.04
0.52
1.1
15
14
19
19
17
26
18.33
4.274
2.137
18.26
7
8
9
9
8
7
8
0.89
0.44
0.8
14
13
15
15
14
13
14
0.89
0.44
0.8
Test
Before
10
12
11
10
10
11
10.66
0.81
0.40
0.91
After
18
18
19
17
16
20
18
1.41
0.70
0.66
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Test
Before
21
22
23
22
20
24
22
1.41
0.70
0.66
After
73
75
74
71
72
70
72.5
1.87
0.93
2.91
Test
Before
51
55
50
50
52
48
51
2.36
0.96
5.6
After
19
20
21
19
21
20
20
0.89
0.36
0.8
Table 6: Skeletal Muscle Relaxant effect of MEHR on rat using Rota-rod model
For Skelatal muscle relaxant: Rota rod model (time required to fall down)
Sr. No.
Group
Control (seconds)
Standard (seconds)
1
Head
240
5
2
Tail
250
6
3
Back
260
8
4
head tail
250
5
5
back tail
240
7
6
No mark
240
4
Average
246.66
5.83
SD
8.16
1.47
SEM
3.34
0.60
variance
66.66
2.16
Test (seconds)
17
15
21
17
18
17
17.5
1.97
0.80
3.9
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Chart 1: Sedative effect of MEHR on rat using Phenobarbital induced sleep model
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Chart 4 a: Anxiolytic effect of MEHR on rat using Light Dark Field model
Chart 4 b: Anxiolytic effect of MEHR on rat using Light Dark Field model
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Chart 6: Skeletal Muscle Relaxant effect of MEHR on rat using Rota-rod model
DISCUSSION:
The result of our study shows methanolic
extract of flower of Hibiscus rosa-sinensis can
markedly reduce fall of time (in Rota-rod
model), decrease in onset of time as well as
increase in duration of action (in phenobarbital
induced sleep model), decrease number of cutoff (in Actophotometer) and increase in number
of crossing (in open-field behavior model),
increase time spent in light field as well as
increase number of entry in close and open arm
(in elevated plus model).
In conclusion, our data indicates that MEHR
can possess Sedative, anxiolytic, CNS depressant and skeletal muscle relaxant
activities.
The MEHR contains flavanoids (hibiscitin),
phenolic content as well as terpenoid
compounds like sitosterol, caemphesterol,
etc, which are probably responsible for the
actions.[5]
CONCLUSION:
The methanolic extract of Hibiscus rosasinensis can increase the duration of action so it
possesses sedative effect.It can also increase the
number of crossing in open field model,
increase the time spent in open arm, entry in
open arm (in elevated plus model) as well as
increase the time spent in light field (in Light
REFERENCES:
ACKNOWLEDGEMENT
On the occasion of presenting this paper in
internet journal, I take this as a unique
opportunity to record my deep sense of
gratitude to my esteemed research guide Tirgar
PR, Professor and Head, Department of
Pharmacology, R. K. College of Pharmacy,
Rajkot and also to Dr. Desai TR, Principal, R.
K. College of Pharmacy, Rajkot.
I am also thankful to Pandiya DJ and Sheth DB
for his kind support for authentication and
identification of this plant.
[1] Nandkarni AK.; Indian metrica medica,volume one,
Bombay,popular prakashan; Vol I: Pg: 634-651;
Edition: 3rd , 2005.
[2] Kokate CK, Purohit AP, Gokhale SB. 1996. Text
book of pharmacognosy, Nirali Prakashan, Pune.
[3] Kulkarni SK; Arch. Int. Pharmacodyn; p.252,124132; 1981
[4] Goyal RK ,Practicals in pharmacology; 6th edition,
Ahmadabad; BS Shah prakashan; 135-138; 2007
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