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The Effect of Composition Co:Cr Variation To Mechanical Properties and Cytotoxicity Co-Cr-HA Composite
The Effect of Composition Co:Cr Variation To Mechanical Properties and Cytotoxicity Co-Cr-HA Composite
ISBN: 978-979-19096-1-7
Abstract
Sintesis of composite Co-Cr-HA (Cobalt
Composite Implants / CIC) created by varying the
composition of Co: Cr respectively (82%: 18%),
(79%: 21%), (76%: 24%), (73%: 27%), (70%:
30%) of added 5% HA from the Co-Cr alloy.
Manufacturing process involves mixing, pressing
and sintering. The process of mixing ingredients
produced using milling machines with a speed of
600 rpm for 30 minutes and then pressed at a
pressure of 370 MPa and sintering carried out at a
temperature of 1000C for 3 hours. XRD test
results showed that the diffraction pattern of crystal
form and the emergence of phase oxide layer CoO,
Co3O4 and CrO2 on the surface of the CIC result of
sintering processes that do not vacuum. Sample
hardness value of 351.096 13.339 kgf/mm2 to
490.130 76.540 kgf/mm2. Compressive streght
increases with the addition of the composition of
Cr, the highest value indicated the composition of
CIC in the ratio of Co: Cr by 70%: 30% of 102.979
0.153 N/mm2. Test on fibroblast cells by MTT
assay method obtained results that the percentage of
living cells with the highest composition of
95.837%. Overall characterization of CIC showed
good results, it is shown by the percentage of living
cells is still above the tolerance limit of 60%.
Introduction
Methodology
2.1
Process Samples
Sample preparation in this process involves mixing
powders Co, Cr and HA. Material is weighed
according to the composition in Table (1) and then
mixed using a milling machine equipped with speed
control device that is dimmer. The addition of
alcohol is very helpful in smoothing the mixture,
Aminatun, The Effect of Composition Co:Cr Variations to Mechanical Properties and Cytotoxicity Co-Cr-HA
Composite
Co (%)
82
79
76
73
70
Cr (%)
18
21
24
27
30
x 100 %
OD treatment + OD mediacontr ol
OD cellcontro l + OD mediacontr ol
HA (%)
5
5
5
5
5
Result
3.1
The test of X-ray Diffractometer (XRD)
XRD test was conducted to identify synthesized
compounds through a search match using the
program Crystal Impact analyst. Results of search
match XRD data shown in Figure (1), (2), (3), (4)
and (5)
2.2
Cytotoxicity testing
Cell BHK 21 (Baby Hamster Kidney 21) clone 13
is saved at temperature of 85C in Nunc vials with
eagle medium, carried out thawing stored is placed
in an incubator at 37C to melt . Then centrifuged
at 3000 rpm for 10 minutes, the precipitate is a cell
culture; storage media are located on the top and
discarded. 20 ml of cell culture is inserted into a
small roux bottle added by grower medium of eagle
and also added by 10% of calf serum, and then
inserted into a CO2 incubator at 37C for 2 x 24
hours, cells will be full.
Cell cultures were divided into micro plate 96 well
each 1.5 ml plus eagle medium and 10% of bovine
serum, then put in a CO2 incubator with a
temperature of 37C for 2x24 hours. Once
confluent the media was removed and washed 2
times with PBS solution, monolayer culture which
is clean is then added back with the eagle media
and the EMS 5% which was mixed with a sterile
CIC sample. Another one that is used as a control is
cell culture medium without CIC compound, and
then all of a micro plate is stored again in incubator
CO2 for 24 hours.
P002
3rd International Conferences and Workshops on Basic and Applied Sciences 2010
3.2
ISBN: 978-979-19096-1-7
Mechanical properties
Sample
Vickers Hardness/
VHN (kgf/mm2)
371,463 12,404
79,589 0,122
II
351,096 23,105
94,627 0,140
III
426,077 85,500
99,152 0,152
IV
490,130 132,572
102,668 0,157
392,888 19,341
102,979 0,153
Compressive
strength
(N/mm)
3.3
Microvickers Hardness
Measurement of hardness value of CIC is using a
hardness tester with a suppressor on the sample
surface with a 136 angle of diamond pyramidshaped. The amount of loads should be greater than
the yield strength of metals to plastic deformation
occurs. VHN values taken on 3 (three) points of the
sample surface so that CIC hardness values
obtained as in Table (2) and Figure 6.
Figure 4: Search match curve CIC-IV
G
rafik Kekerasan
CIC
adap
Vickers
Hardness
Vsterh
Composition
Variasi komposisi Co-Cr
VHN
(kgf/mm)
Co-Cr Variation
600
500
IV
III
400
II
300
200
100
CIC
0
0
3.4
Compressive strength
Compressive strength is the maximum stress that
can be accepted by a material in the form of
compression or tension without failure. The tools
used are autograph equipped with a Control /
Measure Unit. This tool provides a force from
above or from below the surface of the sample so
that the magnitude of P (force) when the specimen
P002
Aminatun, The Effect of Composition Co:Cr Variations to Mechanical Properties and Cytotoxicity Co-Cr-HA
Composite
Citotoxicity Vs Composition
Co-Cr Variation
%Live cell
G
rafik Kekuatan
TekanVs
CIC
Terhadap
Compressive
strength
Composition
Variasi komposisi Co-Cr
Co-Cr Variation
(N/mm)
120
100
IV
III
II
80
60
40
20
0
CIC
0
3.5
The results of cytotoxicity test
Elisa Reader readings indicate that the CIC is not
toxic to fibroblast cells (cell lines), this is indicated
by the percentage of living cells is still above 60
percent ( 60%), the optical density of the treatment
is still close to the optical density of control.
Optical
Density
(OD)
0,177 0 033
0,191 0 036
0,209 0 034
0,221 0 040
0,266 0 043
Conclusions
% Living Cells
(OD)
78,606
81,317
84,947
87,294
95,837
References
[1] Black, Jonathan, Hastings, Garth, Handbook
of Biomaterials Properties, First Edition,
London: Chapman and Hall, 1998.
P002
3rd International Conferences and Workshops on Basic and Applied Sciences 2010
P002
ISBN: 978-979-19096-1-7