Safety Data Sheet

According to EC Directive 91/155/EEC

Date of issue:
Supersedes edition of

1.

26.05.2004
04.05.2003

Identification of the substance/preparation and of the company/undertaking

Identification of the product
Catalogue No.:

100209

Product name:

Acrylamide for electrophoresis special quality for molecular

biology

Use of the substance/preparation

Biochemical research/analysis

Company/undertaking identification

2.

Company:

Merck KGaA * 64271 Darmstadt * Germany * Phone: +49 6151 72-0

Emergency telephone No.:

Please contact the regional Merck representation

in your country.

Composition/information on ingredients
CAS-No.:
M:
Formula Hill:
Chemical formula:

3.

79-06-1
71.08 g/mol
C3H5NO
CH2CHCONH2

EC-Index-No.:
EC-No.:

616-003-00-0
201-173-7

Hazards identification
May cause cancer. May cause heritable genetic damage. Also harmful by inhalation and in contact with
skin. Also toxic if swallowed. Irritating to eyes and skin. May cause sensitization by skin contact.
Also toxic: danger of serious damage to health by prolonged exposure through inhalation, in contact
with skin and if swallowed. Possible risk of impaired fertility.
Restricted to professional users. Attention Avoid exposure - obtain special instructions before use.

4.

First aid measures

First-aid personnel: ensure self-protection!
After inhalation: fresh air.
If breathing stops: immediately apply mechanical ventilation, if necessary oxygen mask.
Immediately call in physician.
After skin contact: wash off with plenty of water. Dab with polyethylene glycol 400.
Immediately remove contaminated clothing. Call in physician.
After eye contact: rinse out with plenty of water with the eyelid held wide open. Call in
ophtalmologist.
After swallowing: make victim drink plenty of water, induce vomiting. Immediately call in
physician.

The Safety Data Sheets for catalog items are also available at www.chemdat.info

Page 1 of 7

Merck Safety Data Sheet

According to EC Directive 91/155/EEC
Catalogue No.:
Product name:

5.

100209
Acrylamide for electrophoresis special quality for molecular biology

Fire-fighting measures
Suitable extinguishing media:
powder, foam, spray water.
Special risks:
Combustible. Danger of dust explosion. Development of hazardous combustion gases or vapours possible
in the event of fire. The following may develop in event of fire: nitrogen oxides.
Special protective equipment for fire fighting:
Do not stay in dangerous zone without self-contained breathing apparatus. In order to
avoid contact with skin, keep a safety distance and wear suitable protective clothing.
Other information:
Contain escaping vapours with water. Prevent fire-fighting water from entering surface water or
groundwater.

6.

Accidental release measures

Person-related precautionary measures:
Avoid generation and inhalation of dusts in all circumstances. Avoid substance contact. Ensure supply
of fresh air in enclosed rooms.
Environmental-protection measures:
Do not allow to enter sewerage system.
Procedures for cleaning / absorption:
Carefully take up dry. Forward for disposal. Clean up affected area. Avoid generation of dusts.

7.

Handling and storage

Handling:
Notes for safe handling:
Work under hood . Do not inhale substance.

Storage:
Tightly closed. Dry. In a well-ventilated place. Protected from light. At +15C to +25C. Accessible
only for authorized persons.

8.

Exposure controls/personal protection

Specific control parameter
EC
Name
Carcinogenic
mutagenic
Fertility
Sensitization

Acrylamide
C 2:should be regarded as if it is carcinogenic to man
M 2:substance which should be regarded as if mutagenic to
man
R(F) 3:cause concern for humans owing possible developmental
toxic effects
Sh Danger of sensitization of the skin

The Safety Data Sheets for catalog items are also available at www.chemdat.info

Page 2 of 7

Merck Safety Data Sheet

According to EC Directive 91/155/EEC
Catalogue No.:
Product name:

100209
Acrylamide for electrophoresis special quality for molecular biology

Personal protective equipment:

Protective clothing should be selected specifically for the working place, depending on
concentration and quantity of the hazardous substances handled. The resistance of the
protective clothing to chemicals should be ascertained with the respective supplier.
Respiratory protection:

required when dusts are generated. Filter A-(P3)

Eye protection:

required

Hand protection:

In full contact:
nitrile rubber
0.11 mm
> 480 Min.

Glove material:
Layer thickness:
Breakthrough time:

In splash contact:
nitrile rubber
0.11 mm
> 480 Min.

Glove material:
Layer thickness:
Breakthrough time:

The protective gloves to be used must comply with the specifications

of EC directive 89/686/EEC and the resultant standard EN374, for
example KCL 740 Dermatril (full contact), 740 Dermatril (splash
contact). The breakthrough times stated above were determined by
KCL in laboratory tests acc. to EN374 with samples of the recommended
glove types.
This recommendation applies only to the product stated in the safety
data sheet and supplied by us as well as to the purpose specified by
us. When dissolving in or mixing with other substances and under
conditions deviating from those stated in EN374 please contact the
supplier of CE-approved gloves (e.g. KCL GmbH, D-36124 Eichenzell,
Internet: www.kcl.de).
Industrial hygiene:
Immediately change contaminated clothing. Apply skin- protective barrier cream. Wash hands and face
after working with substance. Under no circumstances eat or drink at workplace. Avoid generation of
dusts.

9.

Physical and chemical properties

Form:
Colour:
Odour:

solid
colourless
odourless

pH value
at 50 g/l H2O

Melting point
Boiling point
Ignition temperature
Flash point
Explosion limits

(20 C)

5.0-6.5
84

(33.3 hPa)

125

not available
not applicable
lower

not applicable

upper

Vapour pressure
Relative vapour density
Density
Bulk density

not applicable
(20 C)

0.009
2.45

hPa

(25 C)

1.127

g/cm3
kg/m3

~ 500

The Safety Data Sheets for catalog items are also available at www.chemdat.info

Page 3 of 7

Merck Safety Data Sheet

According to EC Directive 91/155/EEC
Catalogue No.:
Product name:

100209
Acrylamide for electrophoresis special quality for molecular biology

Solubility in
water
Thermal decomposition
log Pow:
Bioconcentration factor

(25 C)

2040

g/l

175-300

-1.24

(experimental)

1.44

10. Stability and reactivity

Conditions to be avoided
Strong heating.

Substances to be avoided
acids, alkalis, oxidizing agent, reducing agents.

Hazardous decomposition products

in the event of fire: See chapter 5.

Further information
dust explosion possible, highly reactive.
tendency towards spontaneous polymerization polymerization initiator: warmth/heat and light.

11. Toxicological information

Acute toxicity
LD50 (dermal, rabbit): 252 mg/kg.
LD50 (oral, rat): 124 mg/kg.
Specific symptoms in animal studies:
Eye irritation test (rabbit): Irritations.
Skin irritation test (rabbit): Irritations.

Subacute to chronic toxicity

Animal experiments performed under conditions comparable with the workplace situation have shown the
substance to be carcinogenic.
Animal experiments suggest that the substance may lead to an impairment of reproductive performance
also in man.
A mutagenic effect has been demonstrated in animal studies on mammals, justifying the assumption
that exposure of humans to the substance produces hereditary damage.
Bacterial mutagenicity: Salmonella typhimurium: negative.
Bacterial mutagenicity: Escherichia coli: positive.
No teratogenic effect in animal experiments.

The Safety Data Sheets for catalog items are also available at www.chemdat.info

Page 4 of 7

Merck Safety Data Sheet

According to EC Directive 91/155/EEC
Catalogue No.:
Product name:

100209
Acrylamide for electrophoresis special quality for molecular biology

Further toxicological information

After inhalation: Irritations of the mucous membranes, coughing, and dyspnoea.
After skin contact: Irritations. Danger of skin absorption. Risk of skin sensitization.
After eye contact: Irritations.
After swallowing: irritations of mucous membranes in the mouth, pharynx, oesophagus and
gastrointestinal tract.
After absorption: CNS disorders, ataxia (impaired locomotor coordination).

Further data
This substance should be handled with particular care.

12. Ecological information

Abiotic degradation:
air: Rapid degradation.
Biologic degradation:
Biodegradation: 100 % /28 d (closed bottle test);
Readily biodegradable.
Behavior in environmental compartments:
Distribution: log p(o/w): -1.24 (experimental);
No bioaccumulation is to be expected (log P(o/w <1).
Ecotoxic effects:
Biological effects: Harmfull effect on aquatic organisms.
Fish toxicity: L.macrochirus LC50: 100 mg/l /96 h;
Onchorhynchus mykiss LC50: 110 mg/l /96 h,
Daphnia toxicity: Daphnia magna EC50: 98 mg/l /48 h;
Algeal toxicity: Selenastrum capricornutum IC50: 72 mg/l;
Bacterial toxicity: Photobacterium phosphoreum EC50: 13500 mg/l.
Further ecologic data:
Do not allow to enter waters, waste water, or soil!

13. Disposal considerations

Product:
Chemicals must be disposed of in compliance with the respective national regulations. Under
www.retrologistik.de you will find country- and substance-specific information as well as
contact partners.

Packaging:
Merck product packaging must be disposed of in compliance with the country-specific regulations
or must be passed to a packaging return system. Under www.retrologistik.de you will find special
information on the respective national conditions as well as contact partners.

The Safety Data Sheets for catalog items are also available at www.chemdat.info

Page 5 of 7

Merck Safety Data Sheet

According to EC Directive 91/155/EEC
Catalogue No.:
Product name:

100209
Acrylamide for electrophoresis special quality for molecular biology

14. Transport information

Road & Rail ADR, RID
UN 2074 ACRYLAMID, 6.1, III
Inland waterway ADN, ADNR not tested
Sea IMDG-Code
UN 2074 ACRYLAMIDE, SOLID, 6.1, III
Ems
F-A S-A
Air CAO, PAX
ACRYLAMIDE, 6.1, UN 2074, III
The transport regulations are cited according to international regulations and in the form
applicable in Germany . Possible national deviations in other countries are not considered.

15. Regulatory information

Labelling according to EC Directives
Symbol:
R-phrases:

S-phrases:

53-45

EC-No.:

201-173-7

Toxic

45-46-20/21-25-36/38-43-48/23/24/25-62
May cause cancer. May cause heritable genetic
damage. Also harmful by inhalation and in contact
with skin. Also toxic if swallowed. Irritating to
eyes and skin. May cause sensitization by skin
contact. Also toxic: danger of serious damage to
health by prolonged exposure through inhalation, in
contact with skin and if swallowed. Possible risk
of impaired fertility.
Avoid exposure - obtain special instructions before
use. In case of accident or if you feel unwell,
seek medical advice immediately (show the label
where possible).
EC label

Reduced labelling (1999/45/EC,Art.10,4)

Symbol:
R-phrases:

S-phrases:

53-45

Toxic

45-46-20/21-25-43-48/23/24/25-62
May cause cancer. May cause heritable genetic
damage. Also harmful by inhalation and in contact
with skin. Also toxic if swallowed. May cause
sensitization by skin contact. Also toxic: danger
of serious damage to health by prolonged exposure
through inhalation, in contact with skin and if
swallowed. Possible risk of impaired fertility.
Avoid exposure - obtain special instructions before
use. In case of accident or if you feel unwell,
seek medical advice immediately (show the label
where possible).

16. Other information

Reason for alteration
General update.

Regional representation:
This information is given on the authorised Safety Data Sheet
for your country.

The Safety Data Sheets for catalog items are also available at www.chemdat.info

Page 6 of 7

Merck Safety Data Sheet

According to EC Directive 91/155/EEC
Catalogue No.:
Product name:

100209
Acrylamide for electrophoresis special quality for molecular biology

The information contained herein is based on the present state of our knowledge. It
characterizes the product with regard to the appropriate safety precautions. It does not
represent a guarantee of the properties of the product.

The Safety Data Sheets for catalog items are also available at www.chemdat.info

Page 7 of 7