Professional Documents
Culture Documents
Panjab University: RD TH
Panjab University: RD TH
2011-2012
Subject
Scheme of Teaching
Scheme of Examination
Theory/ Practical
Sess.
Univ.
Total
Exam.
50
50
100
Hrs
Credits
50
50
BIO302
Concepts in BioTechnology-I
Concepts in BioTechnology-I
(Prac.)
Microbiology
50
50
100
BIO352
Microbiology (Prac.)
50
50
BIO303
Biochemistry
50
50
100
BIO353
Biochemistry (Prac.)
50
50
BIO304
50
50
100
50
50
50
50
100
Mathematics-III
50
50
100
Total
22
10
34
30
500
300
800
BIO301
BIO351
BIO354
BIO305
AS301
Sub
Code
BIO401
BIO451
BIO402
BIO403
BIO453
BIO404
BIO454
BIO405
BIO455
BIO406
Subject
Scheme of Teaching
Scheme of Examination
Theory/ Practical
Sess.
Univ.
Total
Exam.
50
50
100
Hrs
Credits
50
50
50
50
100
50
50
100
50
50
50
50
100
50
50
50
50
100
Concepts
in
Biotechnology-II
Concepts
in
Biotechnology-II
(Prac.)
Process
Thermodynamics
Chemical
Reaction
Engineering
Chemical
Reaction
Engineering (Prac.)
Industrial
Microbiology
Industrial
Microbiology (Prac.)
Immunology
&
Immuno-technology
Immunology
&
Immuno-technology
(Prac.)
Legal Laws and IPR
50
50
50
50
100
Total
24
32
28
500
300
800
Practical
Theory
Paper
Code
Paper Title
BIO501
Enzyme Engineering
Technology
Bio-Process Engineering
Animal Cell Culture
Biotechnology
Transport Phenomena
Bio-Process Technology
Bioinstrumentation
BIO502
BIO503
BIO504
BIO505
BIO506
Hours/
Week
Marks
Uni.
Exam
Int.
Ass.
Hours/
Week
Marks
Uni.
Exam
Int.
Ass.
Practical
Paper
Code
& 4+0
100
50
50
50
BIO551
4+0
& 4+0
100
100
50
50
2
2
50
50
50
50
BIO552
BIO553
4+0
4+0
4+0
100
100
100
50
50
50
2
---
50
---
50
---
BIO554
---
50
600
300
08
200
250
Int.
Ass.
50
Practical
Paper
Code
BIO651
Practical
Paper Title
Recombinant
DNA
Technology
BIO602
Operation Research
BIO603
Introduction
to
BioInformatics
BIO604
Bioreactor
Design
and
Operation
BIO605
Down Stream Processing
BIO606
Biomaterials
Grand Total: 1300
Hours/
week
Int.
Ass.
Hours/
Week
4+0
Marks
Uni.
Exam
100
50
Marks
Uni.
Exam
50
3+1
4+0
100
100
50
50
2
2
50
50
50
50
BIO652
BIO653
4+0
100
50
--
--
--
--
4+0
4+0
24
100
100
600
50
50
300
3
-9
50
-200
50
-200
BIO655
--
Practical
Paper Title
Hours/
week
Environmental
Biotechnology
Food Biotechnology
Plant Tissue Culture
Bioanalytical Techniques
Minor Project
Training of 4 weeks after
6th semester exams
Int.
Ass.
Hours/
Week
4+0
Marks
Uni.
Exam
100
Int.
Ass.
Marks
Uni.
Exam
50
50
Practical
Paper
Code
BIO751
50
4+0
4+0
4+0
--
100
100
100
--
50
50
50
--
50
50
BIO752
2
5
50
75
50
75
50
BIO754
--
16
400
200
11
225
275
Option 1
Option 2
Theory
Paper
Code
BIO801
BIO802
Practical
Paper Title
Hours/
Week
Major Project
-Enzyme
catalyzed 4+0
organic synthesis
Marks
Uni.
Exam
-100
Int.
Ass.
Hours/
Week
-50
5
3
Marks
Uni.
Exam
150
50
150
50
Practical
Paper
Code
-BIO852
50
50
BIO854
--
BIO803
4+0
100
50
BIO804
4+0
100
50
BIO805
Elective I *
4+0
100
50
--
--
--
16
400
200
11
250
250
650
450
Options in Elective - I*
1. Nanobiotechnology
2. Microbial Biodiversity
Int.
Ass.
The student got selected for job in campus placement and the employer is
willing to take that student for the training.
b. The student got offer of pursuing training from reputed government research
organization/govt. sponsored projects/govt. research institution provided that
student should not be paying any money to get trained. For pursuing this training
student needs the prior approval from the Coordinator of the respective branch.
SYLLABUS
B.E. IN BIOTECHNOLOGY
THIRD SEMESTER
Paper Title: Concepts in Biotechnology-I (Theory)
Paper Code: BIO 301
Max. Marks 50
Credits: 4
New York.
b. Separation, Recovery and Purification in Biotech by Juan H., and Sengo, A.A., 1985,
American Chem. Society, Washington.
c. Lab. Techniques in Biochemistry and Molecular Biology, 1984, Elsevier, New York.
e. Physical Biochemistry,1982, Friefelder Freeman and Co., New York.
Credits: 1
Practicals:
1.
2.
3.
4.
Credits: 4
SECTION-A
1.
2.
3.
1.
2.
3.
4.
5.
Books Recommended:
Microbiology by Pelczar M.J. and Chan. E.C.S.(Jr.), Reprint,2008, Tata
McGraw Hill Pub. Co., New Delhi.
Medical Microbiology by Bernard, D.D., Dulbecco, R., Eisen, H.N., and
Ginsbery, H.S., 1990, Harper and Row, New York.
Industrial Microbiology by Waites M.J. et al., 2001, Blackwell Science Ltd.,
London.
Instant Notes in Microbiology by Nicklin J. et al., 2002, BIOS Scientific
publishers, Ltd., U.K.
Microbiology: An Introduction by Tortora Benjamin/Cummings Pub. Co.,
10th edition, 2009.
Credits: 4
2.
e) nucleic
3.
4.
5.
6.
7.
8.
10.
Fat metabolism: Oxidation of fatty acids, synthesis of fatty acids (fatty acid
synthase complex), ketone bodies.
Amino acid metabolism: General pathways of amino acid metabolism ,
transamination, decarboxylation , deamination , Urea cycle.
Nucleic acid metabolism: Biosynthesis of purines and pyrimidine nucleotides,
biosynthesis of deoxyribonucleotides , their regulation , catabolism.
Mitrochondria: Structure of mitochondria, organization of respiratory chain,
oxidative phosphorylation .
Plant and microbial biochemistry- Photosynthesis and Nitrogen fixation
Books Recommended:
a) Principles of Biochemistry, Lehninger, A.L., Nelson, DL and Cox, M. M. 4th revised
edition, 2008, Worth Publishers, New York
b) Harper's Biochemistry by Murray, R.K, 2006, 28thedition, 2008, Mc Graw Hill,
America.
c) Biochemistry by Zubay, G., 4thedition,1999, W..C.Brown Publishers, Oxford, England.
d). Biochemistry by Stryer, L., 2006, 6th edition W.H. Freeman and Company, New
York.
e) Biochemistry by Voet, D and Voet J.G., 2006,3rd edition, John Wiley and Sons, Inc.
New York.
f) Outlines of biochemistry by Conn, E.C. and Stumph, P.K. 5th edition, 2006, John
Wiley & Sons.
Max. Marks: 50
Credits: 4
SECTION-B
Transcription in Prokaryotes and Eukaryotes: Mechanism, factors in prokaryotes and eukaryotes,
regulatory mechanisms like substitution of sigma factor, Lac operon in prokaryotes, regulation of
gene expression in eukaryotes, post-transcriptional changes in eukaryotes, RNA editing.
(8)
Translation in Prokaryotic and Eukaryotic cells: stages, molecules involved differential
translation.
(6)
Cell Cycle and its Regulation: Phases of cell cycle, cell cycle check points, brief introduction to
factors involved
in
cell
cycle regulation.
(4)
Signal Transduction: Signal transduction through receptor interacting with G-protein/ receptor
with tyrosine kinase activity, role of second messengers like c-AMP, Inositol- tri- phosphate,
Diacyl glycerol,
Ca2+ ions
and
mechanisms.
(4)
Role of molecular Biology in Molecular Medicine.
(1)
Books Recommended :
Latchman,D., 2006
: Basic Molecular and Cell Biology(2009). Black Well pub.,
3rd edition.
Lewin, B., 2003
: Gene VIII, Oxford University Press, New York.
Stansfield, W. D., Colome, J.S.
: Schaums Outline of Theory and Problems of Molecular
and Cano, R. J., 2004
and Cell Biology, Tata McGraw Hill Edition, 1996, TMH.
Glick, B.R. and Pasternak, J. J., : Molecular Biotechnology, Principles and applications of
2004
recombinant DNA, Second edition, American Society for
Microbiology Press, Washington D.C.
Kendrew, S.J., 1994
: The Encyclopedia of Molecular Biology, Blackwell, U.K.
Credits: 4
Sequences and Series: Sequences, Limits of sequences, Infinite series, series of positive
terms, Integral test, Comparison test, Ratio test, Root test. Alternating series, Absolute
and Conditional Convergence, Leibnitz test. Power series: radius of convergence of
power series, Taylors and Maclaurins Series, Formulae for remainder term in Taylor
and Maclaurin series, Error estimates. (Scope as in Chapter 8, Sections 8.1 8.10 of
Reference 2).
(8 Lectures)
Linear Algebra: Concept of linear independence and dependence, Rank of a matrix:
Row Echelon form, System of linear equations: Condition for consistency of system of
linear equations, Solution by Gauss elimination method. Inverse of a matrix: Gauss
Jordan elimination method (Scope as in Chapter 6, Sections 6.3 6.5, 6.7 of Reference
1).
(7 Lectures)
Eigen values, eigen vectors, Cayley Hamilton theorem (statement only). Similarity of
matrices, Basis of eigenvectors, diagonalization (Scope as in Chapter 7, Sections 7.1, 7.5
of Reference 1).
(7 Lectures)
SECTION-B
Complex Functions: Definition of a Complex Function, Concept of continuity and
differentiability of a complex function, Cauchy Riemann equations, necessary and
sufficient conditions for differentiability (Statement only). Study of complex functions:
Exponential function, Trigonometric functions, Hyperbolic functions, real and imaginary
part of trigonometric and hyperbolic functions, Logarithmic functions of a complex
variable, complex exponents (Scope as in Chapter 12, Sections 12.3 12.4, 12.6 12.8 of
Reference
1).
(8 Lectures)
SYLLABUS
B.E. IN BIOTECHNOLOGY
FOURTH SEMESTER
Paper Title: Concepts in Bio-Technology-II (Theory)
Paper Code: BIO 401
Max. Marks:100
Course Duration: 45 Lectures of one hour each.
Credits: 4
Credits: 4
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The
students will be required to attempt 5 questions selecting at least 2 from each section.
SECTION-A
Introduction and brief review of the Kinetics of Homogeneous reactions
(04)
Interpretation of rate data from Constant volume and Constant pressure systems. (08)
Single ideal reactors
(04)
Design for single reactions
(04)
Biochemical Kinetics: Interpretation of batch kinetic data; kinetics of enzymes catalyzed
reactions in free and immobilized states; Michaelis-Menton equation and its various
modifications;
(04)
SECTION B
Design for multiple reactions
(08)
Biochemical Kinetics: Monod growth model and its various modifications; transport
phenomena in bioprocess systems; Effects of external mass transfer in immobilized
enzymes systems; analysis of intraparticle diffusion and reaction; kinetics of substrate
utilization , product formation and biomass production.
(06)
Thermal Characteristics of Reactors: Temperature and pressure effects.
(07)
Books Recommended:
Chemical Reaction Engineering
:Levenspiel,D(John Wiley), 3rd edition,
1998.
Chemical Engineering or Chemical Kinetics : Smith, J.M.(Mc-Graw Hill) than 3rd
ed.,digitized in 2007.
Reaction Kinetics for Chemical Enginners: Wales, S.M.( Mc-Graw Hill), 3rd edition.
Chemical Reactor Theory - An introduction : Denbigh,K.Turner,K.K.(Cambridge Press)
3rd rev. edition., C U P Archive.
Elements of Chemical Reaction Enginnering : Scott Foggler, H.(PHI), 4th edition, 2006
Paper Title: Chemical Reaction Engineering(Practical)
Paper Code:BIO 453
Max. Marks: 50
Credits: 1
1.
2.
3.
4.
5.
6.
7.
Credits: 4
Books Recommended:
1. M. J. Pelczar and E.C.S. (Jr) Chan, 2000 : Microbiology, Tata McGrawHil1
Pub.Co.;New"Delhi,5thed, 1986,digitize d on july-2008.
2. Davis.Bernard.D, R.Dulbecco, H.N.1990 Medical Microbiology, Harper and
Row, New York, 2nd edition.
3. M. J. Waites et al., 2001 : Industrial Microbiology, Blackwell Science Ltd.,
London, 3rd edition.
4. Nicklin et al., : Instant Notes in Microbiology, BIOS Scientific Publishers,
Ltd., U.K.
5. G.J.Tortora et al.1995:Microbiology: An Introduction. Benjamin/Cummings
Pub. Co., Inc, 10th ed., 2009.
Credits: 1
Practicals :
1. Microbial cell growth kinetics.
2. Determination of size and density of the microbial cells; determination of thermal
death rate constant.
3. Estimation of efficiency of a glass-wool air-filter; preparation of immobilized
whole cell system,.
4. Production of ethanol, acids, solvents in microbial systems.
Credits: 4
1.
2.
3.
4.
5.
6.
7.
8.
9.
SECTION-A
Introduction and historical perspectives of immune system.
(2)
Cell and Tissues of immune system: Lymphoid cell, mononuclear cell,
granulocytes, mast cells, dendritic cells, primary lymphoid organs, lymphatic
system, secondary lymphoid organs.
(4)
Antigens and Haptens: Immunogenecity, chemical composition, susceptibility to
antigen processing, immunogen dosage and route of administration, haptens,
adjuvants.
(4)
Antibody Structure, Function and Antibody Diversity: Basic structure,
Immunoglobulin domains, classes.
(6)
Major Histocompatiblity Complex: MHC molecules, cellular distribution, general
importance.
(3)
Antigen processing and Presentation to T cell: Antigen presenting cells and their
role, pathway.
(4)
SECTION-B
Antigen recognition and activation of immune response.
(4)
Autoimmunity : Organ specific and systematic autoimmune diseases.
(3)
Hypersensitive Reactions: Types, mechanisms of hypersensitivity.
(3)
Books Recommended:
1. Goldsby, Kindt, T.J., and Osborne, B. A. 2000 : Kuby Immunology, W.H.
Freeman & Co., NY. 6th edition, 2007.
2. George Pinchuk, 2004 : Schaums Outline of Theory and Problems of
Immunology, Tata McGraw Hill Edition, 1st edition, 2001.
3. Abbas, A. K., Lichtman, A. H. & Pober, J. S., 2000: Cellular and
Mol.Immunology,. Philadelphia, 6th Edition., 2007, U.S.A, digitized on June
2008, Saunders Elsevier.
4. I.M. Riott, 2000 : Essential Immunology, ELBS/ Blackwell Scientific
Publications, U.K, 11th edition, 2006.
Paper Title: Immunology and Immunotechnology (Practical)
Paper Code: BIO 455
Max. Marks:50
Credits: 1
Practicals:
1. TLC and DLC for blood samples.
2. Determination of cell number (viable/non-viable).
3. Ficoll density gradient, separation of cell types.
4. Immunization methods: Preparation of polyclonal antibodies.
5. Determination of blood group antigens by hemeagglutination assay
6. Radial immunodiffusion Assay.
7. Ouchterlony Double Diffusion Assay.
8. Characterization of immunobiologicals by ELISA.
Credits: 4
SYLLABUS
B.E. IN BIOTECHNOLOGY
FIFTH SEMESTER
Paper Title: Enzyme Engineering & Technology (Theory)
Paper Code: BIO501
M. Marks: 100
Time: 3 hrs
M. Marks: 50
Time: 2 hrs
Time : 3 Hrs.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
Section - A
Kinetics of Microbial growth, substrate utilization and product formation in Batch, Fedbatch and continuous processes.
(10)
Rheology of fermentation fluids and Scale-up concepts.
(05)
Introduction to modeling of growth kinetics: General structure for kinetic models,
overview of structure and unstructured models.
(08)
Section - B
Sterilization of media: design of heat sterilization processes; kinetics; Sterilization in
place and Cleaning in place concepts.
(06)
Sterilization of air : Filter sterilization and kinetics.
(04)
Design of fermentation media and optimization.
(06)
Aeration and agitation : various correlations and mass-transfer aspects, kLa
determination.
(06)
Paper Title : Bio-Process Engineering (Practical)
Paper Code : BIO 552
Max. Marks : 50
Time : 2 Hrs.
2. . M.L.Shuler , F.Kargi
3. Nielson J. Villadsen J.
5. M. Moo. Young
M. Marks : 100
Time: 3 hrs
M. Marks: 50
Time: 2 hrs
Books Recommended:
1.
2.
Animal Biotechnology Vol 1 and II by Griffith and Spier, 6th edition, 1994,
Acadamic Pr.
Animal Cell Culture by Freshney, 3rd edition, 2009, digitized on Feb-2010.
Time 3 hours
SECTION-A
Transport of momentum, heat and mass by molecular motion-Newtons law of viscosity,
Fouriers Law of heat conduction, Ficks Law of Diffusion.
Transport properties-Viscosity, Thermal Conductivity and mass diffusivity.
Development of mathematical models of transfer processes through shell momentum
balance for solving specific problems of transport of momentum in laminar flow or in
solids in one dimension.
Development of general differential equations of fluid flow and their applications in
solving one-dimensional steady state and unsteady state problems of momentum transfer.
Emphasis on the analogy between momentum heat and mass transfer with respect to
transport mechanism and governing equations.
SECTION -B
Development of mathematical models of transfer processes through shell energy balance
and shell mass balance for solving specific problems of transport of heat and mass in
one dimension.
Development of general differential equations for heat transfer and mass transfer
and their applications in solving one-dimensional steady state and unsteady state
problems of heat and mass transfer.
Dimensional Analysis.
Books recommended:
Transport Phenomena
Fundamentals of momentum
Heat and mass transfer
Transfer
Paper Title: Transport Phenomena (Practical)
Paper Code: BIO 554
Max Marks 50
Time: 2 hrs
M. Marks 100
Time: 3 Hours
Books Recommended:
M. Marks : 100
Time: 3 hrs
Bioelectric Potentials-Electrophysiology
Origins of biopotentials dipoles remote versus local potentials muscle and nerve cells,
intracellular potentials resting potential action potential stimulators discriminators
histograms remote potentials evoked potentials; example: evoked potential audiometry
Electrodes electrode interfaces fluid-fluid salt-fluid metal-fluid surface (skin) electrodes
metal micro-electrodes glass micro-electrodes microelectrode preamplifiers
Recording and Stimulating Systems, Putting it all together interference minimization and
rejection stimulus isolation shielding grounding ground-loops; Sample recording system:
electrocardiogram; ECG instrumentation
Other cardiac devices ; Pacemakers power and pulse sources electrodes; Defibrillators
Introduction to medical imaging
Books Recommended:
1. James Diefenderfer
50 Marks
SYLLABUS
B.E. IN BIOTECHNOLOGY
SIXTH SEMESTER
Paper Title: Recombinant DNA Technology (Theory)
Paper code: BIO601
Max. Marks:100
Time:3Hours
SECTION-A
1. Gene cloning and need to clone a gene; Isolation and purification of plasmid,
chromosomal and genomic DNA from bacterial, plant and animal cells.
(10)
(10)
SECTION-B
3. Cloning of a specific gene; studying gene location and structure; studying gene
expression; expression of foreign genes in research and biotechnology; maximization of
recombinant proteins; brief introduction to sequencing and site directed mutagenesis,
different types of PCR and applications; safety measures and regulations for recombinant
DNA work
(15)
4. A brief introduction to the followings: phage display system, Yeast two hybrid system,
and RNAi technology.
(5)
5. Applications of recombinant DNA technology in the fields of Medicine, Agriculture,
Forensic and Environment.
(5)
Max. Marks:50
Time:2Hours
Books Recommended :
1. Watson, J.D., et al.
(3rd ed.)
2. Brown, T.A. (4th ed.)
3. Watson, J. (5th ed.)
Time
Max. Marks 50
Time: 2 Hours
M. Marks : 100
Time: 3 hrs
Sequence Homology:
Scoring matrices, Local and global alignment concepts, Dot matrix sequence comparison,
Dynamic programming; Statistics of alignment score;
Database searches for homologous sequences- FASTA, BLAST, PSI-BLAST and PHIBLAST;
Multiple sequence alignment: CLUSTALW, PILEUP;
Evolutionary analysis- Concept of phylogeny and trees, Relationship of phylogenetic
analysis to sequence alignment.
Gene prediction:
Gene prediction in prokaryotic and eukaryotes genomes; evaluation of gene prediction
methods.
Section - B
Protein structure prediction:
Prediction of protein secondary structure from the amino acid sequence- ChouFasman/GOR method, JPRED, PSIPRED, PHD;
Prediction of three-dimensional protein structure-Homology-based structure prediction,
Fold recognition and ab initio methods for structure prediction;
Evaluating the success of structure predictions-CASP and CAFASP.
Introduction to the concepts of molecular modeling:
Molecular structure and internal energy; Molecular Mechanics; Energy Minimization and
related methods for exploring the energy surface; Molecular Dynamics, Conformational
analysis;
Use of molecular graphics packages- Rasmol, MOLMOL, Chimera, Pymol, spdbviewer.
Computer Aided drug design:
Max Marks 50
Time: 2 hrs
a) To Study & analyse various biological databases at NCBI, EBI, Expasy, NBRFPIR Nucleic acid sequence databases like Gene Bank, EMBL etc.
Protein sequence databases SWISSPROT, UNIPROT etc.
Time: 3 Hrs.
Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are
to be set.. The students will be required to attempt 5 questions selecting at least 2 from
each section.
.
Section - A
Introduction to reactor design and Kinetics of Bioreactions (review).
(1)
Biological systems: Organism selection; bacterial, yeast and fungal cultures; Effect of
microorganism type and culture characteristics on bioreactor design and operation. (6)
Ideal Reactors: Batch reactor (Closed and fed-batch), continuous reactors; PFTR, CSTR
design equations. Reactors for biomass growth; reactors in series; recycle reactors;
overview of pneumatically agitated bioreactors; Membrane bioreactors and Photo
bioreactors.
(10)
RTD in reactors: Models for non-ideal reactors; Tanks in series and dispersion models.
(6)
Section - B
Instrumentation and control of various parameters in bioreactors for Dissolved oxygen,
foam,pH, temperature, flow, pressure, microbial biomass, CO2
etc;
Methods
of
measuring process variables and control systems: Proportional, Proportional integral and
Proportional integral derivative.
(8)
(4)
4. J.M.Lee
5. B. Atkinson
M. Marks 100
Time: 3 Hours
paper, gel filtration, column, ion exchange, affinity, GLC, HPLC. Dialysis,
ulterafilteration; Product polishing: crystallization and drying.
Paper Title: Down Stream Processing (Practical)
Paper Code: BIO655
Max. Marks 50
Time: 2 Hours
Cell lysis and release of cell contents; Use of centrifugation, ultra centrifugation Ultra
filtration, lypophilization, crystallization, HPLC for biosparation.
Books Recommended:
Bailly & Ollis Biochemical Engg. Academic Press, 2nd ed., TMH.
2.H.Gunzler Handbook of Analytical techniques Wiley Publications, 1st ed. , 2001.
3. H.J.Rehm and G.Reed, Biotechnology Vol. 3, 4, 5 verlar Publishers, 2nd ed.,Wiley
VCH
4. Humphrey, Aiba and Miller, Biochemical Engg. Academic Press
5. Murray Moo-Young Comprehensive biotechnology, Vol. II latest ed. Pergan
Publishers, V-4,1985.
6. Wilson and Golding, A Biologists Guide to Principles & Techniques of Practical
7. Stanbury & Whitteker, Principles of Fermentation Technology, Pergamon Press, 1999,
2nd edition.
M. Marks 100
Time: 3 Hours
Section - B
Cardiovascular implants and extra-coronial devices : Blood clotting, Blood Theology, Blood
Vessels, The Heart, Lungs and Vascular implants, Blood substitutes.
M. marks: 100
Time: 3 hrs
Bioremediation-definitions
and
examples.
(5)
Waste Water Treatment: Aerobic and Anaerobic waste water treatment. Kinetics for
waste water treatment processes. Different type and design of Aerobic and Anaerobic
reactors, Methanogenesis. Emerging biotechnological processes in waste water treatment.
(18)
SECTION-B
safety.
(10)
extraction,
microbial
desulphurization
of
coal.
(7)
in
environment.
(5)
Practical:
To determine BOD & COD levels of the sample. Application of microbial approaches
in the remediation of contaminated soils. Analysis of contaminated and bioremediated
samples.
Reference Books
1. Bruce E. Rittmann & Perry L. McCarty. Environmental Biotechnology: Principles
& Applications (1st Edition), McGraw-Hill, 2000.
2. Michael T. Madigan, John Martinko & Jack Parker. Brock Biology of
Microorganisms (10th Edition). Prentice Hall, 2002.
3. George Tchobanoglous, Franklin L. Burton Editor & H. David Stensel
Wastewater Engineering: Treatment and Reuse (4th Edition). McGraw-Hill, 2002.
4. Comprehensive Biotechnology (Vol. 1-4): M. Y. Young (Eds.), Pergamon Press,
Oxford.
Food Biotechnology
M.Marks: 100
Time: 3 hrs
Note: The question paper should be divided into Section A and Section B Total of 8
questions, 4 from section A and 4 from section B are to be set.. The students will be
required to attempt 5 questions selecting at least 2 from each section
SECTION-A
History of Microorganisms in foods
(2)
Primary sources of Microorganisms in foods
(3)
Synopsis of common food borne bacteria, fungi and yeasts
(6)
Incidence & Behavior of Microorganisms in foods
(3)
Extrinsic & Intrinsic parameters of foods
(4)
Role and Significance of Microorganisms as Single cell proteins, Food value of
Mushrooms, Yeasts, Production of Fermented foods
(3)
SECTION-B
Food types and their physical & chemical properties
Food Spoilage
Food Borne diseases
Food Preservation
Diagnosis of microbial contents of food: Classical & Molecular approach
Food Biosensors
(4)
(3)
(6)
(5)
(4)
(2)
Practical:
Microbiological Examination of food/s. Enumeration and detection of food borne
organisms. Estimation of quality of milk-by dye reduction, direct microscopic count,
Determination of diacetyl, titrable acidity in the milk sample.
Reference books
1. Morten Meilgaard, Gail Vance Civille & B. Thomas Carr. Sensory Evaluation
Techniques (3rd Edition). CRC Press, 1999.
2. Daniel Charles. Lords of the Harvest: Biotech, Big Money, and the Future of
Food (1st Edition). Perseus Books Group, 2001.
3. Adams, M.R. and M.O. Moss. Food Microbiology. Turpin Distribution Service
Ltd., Blackhorse Road, Letchworth, Herts SG6 1HN, UK, 1995, 2nd edition.
4. Gauri Mittal.Food Biotechnology: Techniques and Applications. CRC Press,
1992.
5. Banwart, George J. Basic Food Microbiology, 2nd ed. AVI/ Van Nostrand
Reinhold Publishing Co, 1989.
6. Cliver, D.D. Foodborne Diseases. Academic Press, Inc, 1990.
7. Food and Drug Administration. Bacteriological Analytical Manual (BAM) (8th
Edition). AOAC, Arlington, VA, 1995.
M. Marks: 100
Time: 3hrs
Section A
Introduction,
requirements and
Tissue
Culture
Media and
Techniques.
Cell Culturing.
(4)
(5)
Cellular
Totipotency, Somatic
Embryogenesis,
Synthetic
seeds.
(6)
Haploid Production: Zygotic Embryo Culture: Morphogenesis in the culture of seeds
with partially differentiated embryos.
(5)
Section B
Microsurgical experiments, Morphogenic potential of the embryo callus, practical
applications.
(5)
In-Vitro Pollination and Fertilization: Introduction, Terminology,
In-Vitro
Pollination, Applications.
(5)
Genetic engineering and production of pathogen free plants: Gene expression, genetic
stability, and field performance.
(5)
Introduction, Strategies used to optimize product yield, commercial aspects,
Germplasm storage: Introduction, long term Storage, Short or Medium term storage.
(10)
Reference Books:
1. Adrian Slater, Nigel W. Scott & Mark R. Fowler. Plant Biotechnology: The
Genetic Manipulation of Plants (1st Edition). Oxford University Press, 2003.
2. Roberta H. Smith. Plant Tissue Culture: Techniques and Experiments (2 Edition).
Academic Press, 2000.
3. Lydiane Kyte, John Kleyn & John G. Kleyn. Plants from Test Tubes: An
Introduction to Micropropagation (3rd Edition). Timber Press, 1996.
4. J. Heslop-Harrison, John H. Dodds & Lorin W. Roberts. Experiments in Plant
Tissue Culture (3rd Edition), Cambridge University Press, 1995.
Time: 3 Hours
Section-A
Spectrophotometry(UV and Visible): Principle, single beam and double beam
spectrophotometer, factors influencing the absorption spectra, overview of empirical
rules, solvent perturbation method and difference spectroscopy; various applications of
absorption spectroscopy w.r.t. biotechnology.(05)
Spectrofluorimetry: Principle, significance and various details related to
instrumentation. (03)
Atomic absorption Spectrophotometry: Principle, instrumentation details, various
interferences in atomic absorption spectroscopy and applications.(04)
Section-B
Electron Microscopy: Transmission and scanning electron microscopy, significance of
vacuum, basic instrumentation for TEM and SEM, sample preparation for electron
microscopy. Overview of Atomic force microscopy and tunneling microscopy w.r.t. their
working principle and comparison with other scanning techniques. (05)
Crystallography and X-ray diffraction: Introduction to x-ray and general theory and
instrumentation, Braggs law, various techniques to determine crystal structure.(05)
Radioisotope techniques: radiotracers, units of radioactivity measurement, proportional
and scintillation counters, introduction to autoradiography and nuclear medicine.(06)
Mass Spectroscopic techniques: Introduction to mass-spectroscopy, significance,
instrumentation details of a mass-spectrometer, ionization techniques, single and double
focusing, alternate mass separation techniques- time of flight and quadruple. Interface of
mass-spectra with liquid and gas chromatography (LC-MS and GC-MS)(05)
Practical:
Different experiments to confirm spectroscopic laws, practical demonstration of the
above mentioned techniques.
Suggested Books:
1. Keith Wilson & John Walker
:
Principles and Techniques of Practical
Biochemistry (5th Edition), Cambridge
University Press,2000.
2. William Kemp
:
Organic spectroscopy. English Language
Book Society & The Macmillan Press Ltd,
3rd edition.
3. David Friefelder
:
Physical Biochemistry, Freeman & Co.
New York, 2nd edition.
4. P. Carmona, R. Navarro,A.
:
Spectroscopy of Biological Molecules:
Modern trends( Ist Editon), Kluwer
Academic Publishers, 1997.
SECTION-A
Biocatalysis: Definition of Biocatalysis, advantages and disadvantages of Biocatalysis
over chemical catalysis. Different types of Biocatalysis; microbial ,enzymatic and
enzymatic and immobilized system of Biocatalysis; current industrial biocatalysis with
different enzymes.
Immobilized enzymes
for
Biocatalysis.
different
(15)
approaches.
(8)
SECTION-B
Stereo selective biocatalysts for the synthesis of chiral pharmaceutical intermediate such
as synthesis of ACE inhibitors ,definition, mode of action of inhibitors ,recent
developments synthesis of anticholesterol drug by biocatalysis routs , calcium channel
blocking drugs, potassium channel openers, antiviral.
(22)
Practical:
Optimization of enzymatic catalysis reaction conditions.
Enzymatic catalysis reaction with activators and inhibitors.
Product identification using different analytical technique.
Reference Books
1. Andreas S. Bommarius, Bettina R. Riebel. Biocatalysis: Fundamentals &
Applications, Wiley VCH, 2004.
Note for the paper setter: The question paper should be divided into section A and
section B. Total of 8 questions, 4 from section A and 4 from section B are to be set. The
students will be required to attempt 5 questions selecting at least 2 from each section.
Objective: The course aims at acquitting the students with the subject of project
management and also nature, significance and problems of entrepreneur with special
reference to India.
SECTION-A
Project Formulations and Planning ,Private commercial criteria for project choice, project
cycle, feasibility, marketing feasibility, Financing for Projects and financial feasibility,
Project Implementation. Brief outline of social cost benefit analysis: rationale, UNIDO
and little Mirrlees approaches, UNIDO-IDCAS manual, shadow prices and conversion
factors, applications in India. Planning and scheduling networks, critical path, PERT
model, CPM model, PERT/cost, resource leveling and allocation.
SECTION-B
Entrepreneur- Concept on percent - Functions and clarifications of entrepreneurs Characteristics of entrepreneur - Nature and importance of ,entrepreneur - Entrepreneur
vs. professional manager - Women entrepreneurs. Concept of Entrepreneurship Entrepreneurship and environment-Policies governing entrepreneurs, entrepreneurial
development programmes - Institutions for - entrepreneurship development,
entrepreneurship. Entrepreneurship -Entrepreneurship development in other countries.
Institutions for Entrepreneurial Development - Role of constancy organizations - Role of
financial institutions -Bank finance to entrepreneurs Entrepreneurship development: Role
of development financial institutions.
Books Recommended
1.
UNIDO: Guidelines for Project Evaluation, United Nations, reprinted,1993..
2.
Mannual for the preparation of Industrial Feasibility Studies, United Nations
1995.
3.
Manual for Evaluation of Industrial Projects, United Nations, reprinted on 1993..
4.
IMD little and J.A. Mirrlees: Project Apraisal and Planning in Developing
Countries, 1975.
5.
Prasanna Chandra: Projects: Preparation, Appraisal Budgeting and Control, 7th
edition, TMH.
6.
Vasanta Desai: Dynamics of entrepreneurial development and management, 11th
edition, Himalaya pub.
7.
Vasanta Desai: Entrepreneurial development, and Management, 13th edition,
Himalaya pub.,Harper Collins, edition- Paperback.
8.
Peter F. Drucker: Innovation and development.
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set..
The students will be required to attempt 5 questions selecting at least 2 from each
section.
SECTION-A
Types of kinetic models. Data smoothing and analysis.
(8)
Practical:
To design mathematical models from the provided data.
Reference Books
1. John Ingham, Irving J. Dunn, Elmar Heinzle & J.E. Prenosil. Chemical
Engineering Dynamics: Modelling with PC Simulation (2nd Edition). WileyVCH, 2000.
2. Jonathan B. Snape, Irving J. Dunn, John Ingham & J.E. Prenosil. Dynamics of
Environmental bioprocesses: Modelling and Simulation. Wiley-VCH, 1995.
3. Dieter Behrens & P. Kramer (Eds.). Bioreactors, Downstream Processing,
Process and Reactor Modelling and Bioprocesses. Wiley-VCH, 1988.
4. Dunn. Biological Reaction Engineering. John Wiley & Sons Inc
ELECTIVE - 1
BIO805 Nanobiotechnology
Total number of lectures: 45
Maximum Marks: 100
The question paper should be divided into section A and section B. Total of 8 questions, 4
from section A and 4 from section B are to be set. The students will be required to
attempt 5 questions selecting at least 2 from each section
SECTION A
Cell nanostructure interactions: Surface patterning to control the adhesion of cells
using
nanoscale topography and chemical modifications
Magnetosomes: Magnetotactic bacteria, Magnetite crystals,
Applications
Nanotherapeutics: Nanoshells, Dendrimers , Nanoparticles
Tissue engineering: Significance, Methodology,
Magneto-aerotaxis,
Molecular motors: Surface patterning and the control of motility of the actin/myosin
motor system
Engineered nanopores:
applications of nanopores
Reference Books
1. David S. Goodsell. Bionanotechnology: Lessons from Nature. (1st Edition) WileyLiss, 2004.
2. Bharat Bhushan. Handbook of Nanotechnology (1st Edition). Springer Verlag,
2004.
3. Christof M. Niemeyer & Chad A. Mirkin (Eds.). Nanobiotechnology: Concepts,
Applications and Perspectives, 2004.
ELECTIVE 2
Note for the Paper setter: The question paper should be divided into Section A and
Section B Total of 8 questions, 4 from section A and 4 from section B are to be set..
The students will be required to attempt 5 questions selecting at least 2 from each
section.
SECTION A
Microbial biodiversity: definition and introduction, evolution and diversity of
microorganisms.
(7)
Physiological and metabolic diversity of microorganisms.
(7)
Microbial diversity of major ecosystems.
(8)
SECTION B
Biodiversity & role of microorganisms in plants and animal symbiosis.
Microbial diversity in extreme environments.
Microbial biodiversity, biotechnology and future biodiversity.
(8)
(9)
(6)
Reference Books
1. Jo Handelsman. Biology Brought to Life (Student Version) McGraw-Hill, 2002.
2. Oladele Ogunseitan. Microbial Diversity: Form and function in Prokaryotes.
Blackwell Publishers, 2004.
3. 3. Alan T. Bull (Editor). Microbial Diversity and Bioprospecting. American
Society Microbiology, 2003.