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Chemiluminescence
Chemiluminescence
Basicprinciples,Instrumentation
andclinicalutility
By
Heratsoni
2ndYearResident
Biochemistry
Luminescence
X*---------->X+hv(light)
Chemiluminescence
Luminol + H2O2 ---> oxidised luminol* + H2O
-----> oxidised Luminol + hv (light)
Bioluminescence
seen in firefly
Enhanced chemiluminescence
-In presence of HRP
Iodophenol(R-OH) + H2O2 +luminol
RO*(phenolate radical) +H2O +luminol
Electrochemiluminescence
Electrochemiluminescence video
Principle of CLIA
m-AMPD
(m-MOB-)*=m-oxybenzoate anion
M-MOB- + hv(Light)
ATP+pyruvate
Luciferinluciferase
Reaction
Light
Instumentation
Chemilluminescent
Assay
ELISA
Radioimmunoassay
Nostopsolution
Stopsolutionrequired
(notinkineticassay)
Nostopsolution
Highersensitivity
Lesssensitive(stray
light)
Lesssensitive(?)
Nolightsource(Filter) Lightsourceneeded
Nolightsource
Clinical Utility
Thyroid Tumour
markers
AntiTG
AFP
AntiTPO
CA125II
FreeT3
CA199
FreeT4
CEA
TotalT3
PSA
TotalT4
Serum
TSH
HER2/neu
Anemia
Hormones
Ferritin
VitaminB12 AFP
FSH
hCG
Cardiac
LH
BNP
Progesterone
CKMB
Prolactin
DDimer
Testosterone
Therapeutic
Drug
Monitoring
Carbamazepi
ne
Digitoxin
Digoxin
Gentamicin
Phenytoin
Theophylline
Valproic
Acid
Vancomycin
Cyclosporine
Hepatitis
AntiHBe
AntiHBs
HAVIgM
HBcIgM
HBeAg
HBsAg
HBsAgConfirmatory
HCV
ToRCH
CMVIgG
CMVIgM
RubellaIgG
RubellaIgM
ToxoplasmaIgG
ToxoplasmaIgM
Diabetes
CPeptide
(Serum;Urine)
Insulin