Professional Documents
Culture Documents
Bio Rad Water
Bio Rad Water
Water Safety
Water Safety
Contents
Part 1 Quality Indicators Testing
Total flora
10
11
12-13
14-15
Legionella spp. and Legionella pneumophila Extraction for real-time PCR method 16
Legionella spp. and Legionella pneumophila Alternative real-time PCR method
17
18
19
20
!
New Pseudomonas aeruginosa RAPIDP.aeruginosa alternative method
21
22-23
24-25
This booklet is only an indication provided by Bio-Rad for the use of Bio-Rad products.
It is not a contractual document.
Total flora
EN ISO 6222 (07/1999)
Water quality
Enumeration of culturable micro-organisms.
Colony-count by inoculation
in a nutrient agar culture medium.
Pour-Plate method
Water sample
(preparation)
Ordering Information
Catalog # Description
200 ml x 6 bottles
500 g
68 hrs
22C
Reading/
enumeration
44 hrs
36C
Reading/
enumeration
| 3
Ordering Information
Catalog #
Description
355-4282
18 ml x 60 tubes
DSM + Bromophenol Blue
100 g
18 ml x 60 tubes
Fluoplate 2 Software
Water sample
(dilutions in DSM or EDS)
MUG/EC (microplates)
1 chamber
36-72 hrs
44C
Reading
( = 366 nm - UV light)
UV lamp
UV Observation Chamber
(Wood Lamp)
355-0718
Key Benefits
Statistical control (Xhi2 test)
of the input values
Creation of a database easily
exportable into Excel* or Access*
Multi-language software and reports:
english, french, spanish, italian and
german
*Excel is a trademark of Microsoft
*Access is a trademark of Access Systems
4 |
Ordering Information
Catalog #
Treated or untreated
waste water
Untreated
waste water
Water sample
filtration
Water sample
filtration
Pipetting of
1 ml of water
sample
Description
XplOrer64 System
Full Configuration (220/110V)
XplOrer64 System for up to 64 samples,
delivered with computer, UPS, color
printer, CheckNSafe temperature control
cells, cleaning box and 4 racks
XplOrer64 System
Configuration w/o PC (220V)
XplOrer64 System 220V for up to 64
samples, CheckNSafe temperature
control cells, cleaning box and 4 racks
XplOrer64 System
Configuration w/o PC (110V)
XplOrer64 System 110V for up to 64
samples, CheckNSafe temperature
control cells, cleaning box and 4 racks
Please contact your local representative
Membrane inserting
into
CheckNSafe
E.coli
Membrane inserting
into
CheckNSafe
E.coli
Direct inoculation
CheckNSafe
E.coli
9 ml x 60 tests
Key Benefits
!
New
| 5
Ordering Information
Catalog #
Description
10 ml x 25 tubes
10 ml x 25 tubes
Membrane
Kovacs Reagent
355-5313
NF T90-413 (10/1985)
Water testing
Detection and enumeration of
coliforms and thermotolerant
coliforms
General method by culture in
liquid media (MPN)
15 ml x 2 bottles
Oxidase Disks
355-3832
30 disks
9 ml x 25 tubes
500 g
Schubert/Broth
355-5694
356-9634
8 ml x 25 tubes
500 g
Tergitol 7 (0.2%)/Supplement
356-2632
356-2635
50 ml x 1 bottle
5 ml x 1 vial
24-48 hrs
30C
Standard test
TTC Tergitol 7
Rapid test
TSA
TTC (0.05%)/Supplement
356-2652
356-2655
50 ml x 1 bottle
5 ml x 1 vial
200 ml x 6 bottles
500 g
90 mm x 20 dishes
200 ml x 6 bottles
8 ml x 25 slanted tubes
500 g
Tryptophane/Broth
355-4194
3 ml x 25 tubes
6 |
55 mm x 10 dishes
55 mm x 100 dishes
Trypto-Casein-Soy (TSA)/Agar
356-3884
355-5947
355-5944
356-4554
4-5 hrs
36C
21 hrs
(+21 hrs)
36C
500 g
19-20 hrs
44C
Oxidase test
Oxidase
disks
21 hrs
44C
Indole test
Kovacs
reagent
Red
colonies
10-30
min
BGBLB
BGBLB
or
Schubert
48 hrs
37C
Trouble
gas +
Total
coliforms
24-48 hrs
44C
Trouble
gas +
Thermotolerant
coliforms
Indole test
(Kovacs reagent
in Schubert)
E. coli
Ordering Information
Catalog #
Description
!
New
RAPIDE.coli 2/Agar
for Water Testing
356-3982
55 mm x 20 dishes
+
Membrane
24 hrs
36C
for coliforms and E. coli
Key Benefits
Certified by NF VALIDATION
One single plate for simultaneous
E. coli and coliforms testing
Complete results within 18 4 hrs
at just one temperature of 36C
Good colony coloration contrast
for a rapid reading
No confirmation step required
| 7
Ordering Information
Catalog #
Description
18 ml x 60 tubes
100 g
18 ml x 60 tubes
Fluoplate 2 Software
Water sample
(dilutions in DSM or EDS)
MUD/SF (microplates)
1 chamber
36-72 hrs
44C
Reading
( = 366 nm - UV light)
UV lamp
UV Observation Chamber
(Wood Lamp)
355-0718
Key Benefits
Statistical control (Xhi2 test)
of the input values
Creation of a database easily
exportable into Excel* or Access*
Multi-language software and reports:
english, french, spanish, italian and
german
*Excel is a trademark of Microsoft
*Access is a trademark of Access Systems
8 |
New
Ordering Information
Catalog # Description
Samples poor in
suspended matters (SM)
100 ml protocol
Treated or untreated
waste water
Untreated
waste water
Water sample
filtration
Water sample
filtration
Pipetting of
1 ml of water
sample
XplOrer64 System
Full Configuration (220/110V)
XplOrer64 System for up to 64 samples,
delivered with computer, UPS, color
printer, CheckNSafe temperature control
cells, cleaning box and 4 racks
XplOrer64 System
Configuration w/o PC (220V)
XplOrer64 System 220V for up to 64
samples, CheckNSafe temperature
control cells, cleaning box and 4 racks
XplOrer64 System
Configuration w/o PC (110V)
XplOrer64 System 110V for up to 64
samples, CheckNSafe temperature
control cells, cleaning box and 4 racks
Please contact your local representative
Membrane inserting
into
CheckNSafe
Enterococci
Membrane inserting
into
CheckNSafe
Enterococci
Direct inoculation
CheckNSafe
Enterococci
10 ml x 60 tests
Key Benefits
New
| 9
Ordering Information
Catalog #
Description
BEA/Agar
356-3994
355-5829
356-4184
90 mm x 20 dishes
100 ml x 6 bottles
500 g
TTC (0.2%)/Supplement
356-2665
5 ml x 1 vial
100 ml x 6 bottles
500 g
55 mm x 10 dishes
55 mm x 100 dishes
Membrane
44 hrs
36C
Reading
Transfer the membrane on BEA
2 hrs
44C
Reading/enumeration
10 |
Ordering Information
Catalog #
Description
RAPIDEnterococcus/Agar
355-4409
100 ml x 6 bottles
Membrane
RAPIDEnterococcus
8-10 ml per plate
( = 55 mm)
44 hrs 4 hrs
44C
Key Benefits
Direct identification and
enumeration of Enterococci
No confirmation step required
Ready-to-use medium
Save time and materiel
| 11
Ordering Information
Catalog #
Description
Columbia/Agar
356-4674
500 g
100 ml x 6 bottles
20 ml x 25 tubes
7.5 ml x 25 tubes
500 g
100 ml x 6 bottles
500 g
10 min
80C
24 hrs
37C
Reading
Anaerobic
conditions
Meat-Liver Sulfite Iron Agar
12 |
48 hrs
37C
Reading/enumeration
Pathogens Testing
EN ISO standards
NF EN 26461-1 (07/1993)
Water quality
Detection and enumeration of
spores of sulfito-reducing anaerobic
micro-organisms (Clostridia)
Part 2: Membrane-filtration method
15 min
75C
DRCM
Anaerobic
conditions
44 hrs
37C
Reading/enumeration
Membrane
Anaerobic
conditions
15 min
60C
Reading/enumeration
Confirmation
(Columbia Agar, Nutrient Agar 2.1%)
| 13
Ordering Information
Catalog #
Description
BCYE Biplate/Agar
356-3718
90 mm x 20 dishes
90 mm x 20 dishes
90 mm x 20 dishes
90 mm x 20 dishes
GVPC/Agar
356-3717
356-3719
90 mm x 20 dishes
90 mm x 100 dishes
50 tests
50 tests
MonoFluo Kit
Legionella pneumophila
353-2514
0.2 ml
GVPC
0.2 ml 1/10
for dirty water
GVPC
24 tests
90 mm x 20 dishes
1 chamber
UV Observation Chamber
(Wood Lamp)
355-0718
14 |
BCYE Biplate
Pathogens Testing
NF T90-461/A1 (04/2006):
Water quality
Detection and enumeration of
Legionella spp. and Legionella pneumophila
Method by direct inoculation
and after concentration by membrane filtration
or centrifugation
Water sample
Membrane filtration (1 L)
Concentrate
direct inoculation
0.1 ml
GVPC
heat treatment
0.1 ml
GVPC
acid treatments
0.2 ml
GVPC
8-10 days
36C
Confirmation
of Legionella spp.
or
BCYE with
L-Cysteine
BCYE without
L-Cysteine
48 hrs
36C
| 15
Ordering Information
Catalog #
Description
Aquadien Kit
357-8121
96 tests
Extraction step
Aquadien DNA extraction Kit
NF Validation certified according
Water quality - Detection and quantification of
by concentration and genic amplification
No BRD 07/15 -05/11 ;
Aerosol sample
Also available:
Protocol for clogging samples
Biofilm sample
Water sample
48 tests
Aquadien Kit
Lysis of bacteria using thermal shock
95C
15 min
95C
Key Benefits
High extraction yield
Compatible with all type of water
(drinking water as well as cooling
tower)
NF Validation certified
Ready-to-use reagents
Simple protocol
16 |
Pathogens Testing
Legionella spp. and Legionella pneumophila
Alternative real-time PCR method
Ordering Information
Catalog #
Analysis step
Description
Reagent kits
iQ-Check Quanti Legionella spp. Kit
357-8102
96 tests
96 tests
357-8104
96 tests
96 tests
New
359-3990
359-3893
!
Software
Screening
iQ-Check Screen
Legionella spp.
Quantification
iQ-Check Quanti
Legionella spp.
iQ-Check Screen
L. pneumophila
iQ-Check Quanti
L. pneumophila
Key Benefits
Just 5 hours to the result with no
confirmation
Detection of Legionella spp. and
L. pneumophila in the same assay
A 96-wells format adaptable to all
routine throughput
Ready-to-use reagents
Fully automated results analysis
Reference Material provided
Connected to the Standard
Reference Material
NF Validation certified
| 17
Ordering Information
Catalog #
Description
Reagent
Aquadien Lysis Solution
Please contact your local representative
CIP10-M
Method for the monitoring of airborne micro-organisms,
especially Legionella in aerosols
from cooling towers and droplets
Instruments
CIP10-M* Air Sampler/220V
359-3084
Aerosol sampling
CIP10-M
Real-time NF T90-471
Key Benefits
Simulates human breath
Ergonomic
Portable
Free of cross contamination
Suitable for both culture media
(on-going studies) and real-time
PCR analysis
18 |
Pathogens Testing
Description
16249
16186
Aerosols Testing
16995
Biofilms Testing
16225
Intra-amoebic Legionella
| 19
Membrane
CN Agar
48 hrs
37C
Reading/enumeration
Reading
( = 366 nm - UV light)
UV lamp
Nutrient Agar (water testing)
24 hrs
37C
Acetamide Broth
24 hrs
37C
Nessler reagent
Reading/enumeration
ammonia producing
colonies
Oxidase test
Oxidase disks
King B
5 days
37C
Reading at = 366 nm
UV lamp
Enumeration
fluorescent colonies
20 |
Pathogens Testing
Pseudomonas aeruginosa
RAPIDP.aeruginosa alternative method
Ordering Information
Catalog #
RAPIDP.aeruginosa
Chromogenic agar medium
for the direct detection and enumeration
of Pseudomonas aeruginosa by membrane-filtration
New
Description
Acetamide/Broth
355-4355
5 ml x 25 tubes
CN/Agar
356-3915
355-6034
356-4899
55 mm x 10 dishes
200 ml x 6 bottles
500 g
335-0717
335-0718
Portable UV observation
chamber (without wood lamp)
Wood lamp
King B/Agar
355-5278
Membrane
7 ml x 25 tubes
Nessler Reagent
22-30 hrs
36C
RAPIDP.aeruginosa
356-4194
100 ml x 1 vial
90 mm x 20 dishes
Oxidase Disks
355-3832
box of 30 disks
!
New RAPID'P.aeruginosa/Agar
356-3984
356-4900
55 mm x 20 dishes
500 g
Key Benefits
High specificity for
a direct enumeration
of Pseudomonas aeruginosa
Complete results within 22-30 hrs
Good colony coloration contrast
for a rapid reading
No confirmation step required
| 21
Ordering Information
Catalog #
Description
500 g
25 ml x 6 bottles
500 g
Kligler-Hajna/Agar
355-5378
356-4844
10 ml x 25 slanted tubes
500 g
LDC-ODC-ADH/Broth
355-3725
5 ml x 3 bottles
MKTTn (Muller-Kauffmann
Tetrathionate Novobiocin)/Broth
355-6140
356-4714
No filtration
(if volume < 10 ml)
10 ml x 50 tubes
500 g
500 g
9 ml x 25 tubes
10 ml x 25 tubes
500 g
24-48 hrs
41.5C
First medium
XLD
500 g
500 g
24 hrs
36C
(TSI,
22 |
Pathogens Testing
Water sample
Filtration
(if volume > 10 ml)
Membrane
Buffered Peptone Water
18 hrs
36C
24 hrs
36C
Biochemical
LCD, Kliger-Hajna, urea agar)
and if appropriate
serological confirmation
| 23
Ordering Information
Catalog #
Description
Baird-Parker/Agar
Base Medium
356-4814
500 g
Baird-Parker/Agar
Complete Medium
356-3991
90 mm x 20 plates
90 mm x 20 plates
90 ml x 6 bottles
+ 6 vials supplement
10 ml x 25 tubes
500 g
55 mm x 10 dishes
500 g
Additives
Egg yolk with potassium tellurite
355-4201
5 ml x 1 vial
355-4205
25 ml x 1 bottle
48 hrs
37C
355-6353
355-6356
Enumeration of white
colonies with yellow
halo/Gram+
5 x 50 tests
50 tests
Transfer
the membrane
10 vials
2.5 ml x 1 vial
Confirmation
Latex Pastorex Staph+
Confirmation
Brain Heart Infusion
48 hrs
37C
Coagulation reading:
Rabbit Plasma
24 |
Pathogens Testing
XP T90-412 (06/2006):
Water quality
Detection and enumeration
of pathogenic Staphylococci
Membrane-filtration method
Membrane
or
or
Baird-Parker
Baird-Parker + RPF
48 hrs
37C
48 hrs
37C
Enumeration of black
colonies with clear
halo/Gram+
Confirmation
Baird-Parker + RPF
48 hrs
37C
Enumeration of black
or grey colonies surrounded
by a whitish opaque halo/
Gram+
| 25
Main types
of water
Network Tap
Industrial
Process
Artificial Surface
Bottled
Origins of water
Drinking water
Treated cooling
towers, process,
sanitary water
Natural mineral
and source water
Turbidity
Microbial load
MF
MF
MF
Typical quality
indicators
Total flora
E. coli
Coliforms
Enterococci
Total flora
E. coli
Coliforms
Enterococci
Total flora
E. coli
Coliforms
Enterococci
Total flora
Coliforms
(E. coli)
Typical
pathogens
Legionella
P.aeruginosa
Salmonella
SRA
Legionella
P.aeruginosa
Salmonella
SRA
Legionella
P.aeruginosa
Staph. aureus
Salmonella
SRA
Typical methods
Legends:
SRA = Sulfito-Reducing Anaerobes
MF = Membrane filtration
MPN = Most Probable Number
P/A = Presence/Absence
PCR = Polymerase Chain Reaction
26 |
Legionella
P.aeruginosa
Synthesis
Superficial
(Surface)
Bathing
Waste
Cooling Towers
Aerosols
Lakes, rivers
Coastal sea,
estuarine, inland
water
Residual raw
waters, sludge,
soil, treatment
plant effluents
Non treated
cooling towers
Drops from
cooling towers,
tap water
+++
+++
+++
++++
++
++
++
+++
+++
+++
MF/MPN
MF/MPN
Impedance
MPN/PCR
New ! Impedance
MPN/PCR
PCR
Total flora
E. coli
Coliforms
Enterococci
Total flora
E. coli
Coliforms
Enterococci
Total flora
E. coli
Coliforms
Enterococci
Total flora
Total flora
Salmonella
SRA
Legionella
Salmonella
Legionella
Legionella
| 27
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Life Science
Group
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