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Global Yield of The Supercritical CO2 Extraction From Cordia Verbenacea DC - Anticancer and Antimycobacterial Activities
Global Yield of The Supercritical CO2 Extraction From Cordia Verbenacea DC - Anticancer and Antimycobacterial Activities
ISSN: 0973-1296
PHCOG MAG.
An official Publication of Phcog.Net
LASEFIDEA/ FEA (College of Food Engineering) UNICAMP (State University of Campinas), Caixa Postal
6121, 13083-970 Campinas, So Paulo, Brazil;
Instituto Adolfo Lutz, Laboratrio de Ribeiro Preto, Rua Minas 877, 14085-410, Campos Elseos, Ribeiro Preto,
So Paulo, Brazil;
(6). Pharmacological studies have validated its antiinflammatory activity (6,8). The leaves extracts have
anti-inflammatory and analgesic activities (9). The C.
verbenacea volatile oil, obtained by hydrodistillation,
can inhibit the growth of some gram-positive bacteria
(Bacillus cereus, B. subtillis, Staphylococcus aureus, S.
epidermitis) and some yeasts (Candida albicans, C.
dubliniensis, C. glabrata, C. guillermondii, C.
lusitaneae, C. parapsilosis, C. stellatoidea, C.
tropicallis and Cryptococcus sp.) (10). The Ach
Pharmaceutical Laboratories, one of the largest
pharmaceutical groups of Brazil, launched in June of
2005 the Acheflan after seven years of studies in
partnerships with important Brazilian Universities. The
Acheflan formulated using C. verbenacea essential oil
containing 2.3 2.9 % of alpha-humuleno is indicated
for the treatment of chronic tendonitis and myofascial
pains (11).
Lins et al. 12 identified the presence of some flavonols
such
as
sitosterol,
5-hidroxy-3,6,7,3,4pentametoxyflavone (artemetin) and 5,6-dihidroxy3,3,4,6,7-pentametoxiflavone. The artemetin has
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Pharmacognosy Magazine
ISSN: 0973-1296
PHCOG MAG.
An official Publication of Phcog.Net
40
Pharmacognosy Magazine
ISSN: 0973-1296
PHCOG MAG.
An official Publication of Phcog.Net
41
Pharmacognosy Magazine
ISSN: 0973-1296
PHCOG MAG.
An official Publication of Phcog.Net
42
Pharmacognosy Magazine
ISSN: 0973-1296
PHCOG MAG.
An official Publication of Phcog.Net
Table 1. Influence of temperature and pressure on the global yield from C. verbenacea obtained by
supercritical CO2 Extraction.
Pressure (bar)
Temperature (C)
300
300
100
100
341
200
78
200
200
200
200
30
50
30
50
40
54
40
26
40
40
40
2.87
4.25
1.60
0.47
3.36
3.90
0.11
2.35
3.36
3.35
3.32
0.07
0.11
0.13
2.52
2.65
3.99
43
Pharmacognosy Magazine
ISSN: 0973-1296
PHCOG MAG.
An official Publication of Phcog.Net
Figure 1. Anticancer activity as a function of cancerous cellular ancestries for Cordia verbenacea extract
obtained using CO2 at the indicated conditions.
100 bar / 30 C
75
75
50
50
25
0
UACC.62
MCF.7
NCI.460
K.562
OVCAR
PC0.3
HT.29
786-0
NCI.ADR
-25
-50
-75
-100
100 bar / 50 C
100
Growth, %
Growth, %
100
25
0
UACC.62
MCF.7
NCI.460
K.562
OVCAR
PC0.3
HT.29
786-0
NCI.ADR
-25
-50
-75
-100
10
10
-3
10
-2
10
-1
10
10
2.5
0.25
10
25
10
-3
10
-2
10
-1
10
250
10
2.5
0.25
10
25
10
250
Concentration (g/ml)
Concentration (g/ml)
100
300 bar / 30 C
75
75
50
50
Growth, %
Growth, %
100
25
0
UACC.62
MCF.7
NCI.460
K.562
OVCAR
PC0.3
HT.29
786-0
NCI.ADR
-25
-50
-75
-100
10
-3
10
-2
300 bar / 50 C
25
0
UACC.62
MCF.7
NCI.460
K.562
OVCAR
PC0.3
HT.29
786-0
NCI.ADR
-25
-50
-75
-100
10
10
-1
10
10
2.5
0.25
10
25
10
-3
10
-2
10
-1
10
10
2.5
0.25
10
25
10
250
Concentration (g/ml)
250
Concentration (g/ml)
Figure 2. Anticancer activity as a function of cancerous cellular ancestries for Cordia verbenacea extract obtained by
100
100
75
75
50
50
25
Growth, %
Growth, %
0
UACC.62
MCF.7
NCI.460
K.562
OVCAR
PC0.3
HT.29
786-0
NCI.ADR
-25
-50
-75
-100
10 -3
10 -2
25
0
UACC.62
MCF.7
NCI.460
K.562
OVCAR
PC0.3
HT.29
786-0
NCI.ADR
-25
-50
-75
-100
10 -1
10 0
0.25
10 1
2.5
Concentration (g/ml)
10 2
25
10 3
250
10 -3
10 -2
10 -1
10 0
0.25
10 1
2.5
10 2
25
10 3
250
Concentration (g/ml)
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Pharmacognosy Magazine
ISSN: 0973-1296
3.
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An official Publication of Phcog.Net
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Pharmacognosy Magazine
ISSN: 0973-1296
PHCOG MAG.
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