Product Datasheet

Anti-MUM1 antibody ab104803

1 Abreviews

2 Images

Overview
Product name

Anti-MUM1 antibody

Description

Rabbit polyclonal to MUM1

Tested applications

WB, IHC-P, IHC-Fr

Species reactivity

Reacts with: Mouse, Human

Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish

Immunogen

Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MNLEGGGRGG EFGMSAVSCG
NGKLRQWLID QIDSGKYPGL VWENEEKSIF) of Human MUM1 (NP_002451).
Run BLAST with

Run BLAST with

Positive control
Purchase matching WB positive control:
Human MUM1 full length protein
Jurkat cell lysate; Human skin tissue

Properties
Form

Liquid

Storage instructions

Shipped at 4C. Upon delivery aliquot and store at -20C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Applications
Our Abpromise guarantee covers the use of ab104803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application

Abreviews

WB

Notes
Use a concentration of 0.5 g/ml. Predicted molecular weight: 52 kDa. Good results were obtained when
blocked with 5% non-fat dry milk in 0.05% PBS-T.

IHC-P

Use a concentration of 4 - 8 g/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before
commencing with IHC staining protocol.

IHC-Fr

1/100.

Target
Function

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter.
Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISREtargeted signal transduction mechanisms specific to lymphoid cells.

Tissue specificity

Lymphoid cells.

Involvement in disease

Defects in IRF4 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells
usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system,
hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain,
hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to
impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some
patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown
significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving IRF4 is found in multiple

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myeloma. Translocation t(6;14)(p25;q32) with the IgH locus.
Sequence similarities

Belongs to the IRF family.

Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Cellular localization

Nucleus.

Anti-MUM1 antibody images

Anti-MUM1 antibody (ab104803) at 0.5 g/ml + Jurkat cell
lysate at 10 g

Predicted band size : 52 kDa

Gel concentration: 12%

Western blot - MUM1 antibody (ab104803)

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) analysis of human skin tissue

labelling MUM1 with ab104803 at 8g/ml. Arrows indicate
positively labelled squamous epithelial cells.
Magnification: 400X.

Immunohistochemistry (Formalin/PFA-fixed paraffinembedded sections) - MUM1 antibody (ab104803)

Please note: All products are "FOR RESEARCH USE ONLYAND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery**
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Terms and conditions

Guarantee only valid for products bought direct from Abcam or one of our authorized distributors

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