DOC/LP/01/28.02.

02

LESSON PLAN
BT2023
Unit: I

LP- BT2023

Sub Code & Name:

MOLECULAR PATHOGENESIS

Branch: Biotechnology

Semester : VI

LP Rev. No: 04
Date: 31.12.14
Page 1 of 7

UNIT-1 : OVERVIEW
Historical perspective - discovery of microscope, Louis Pasteurs contributions, Robert Kochs
postulates, early discoveries of microbial toxins, toxic assays, vaccines, antibiotics and birth of
molecular genetics and modern molecular pathogenesis studies, Various pathogen types and
modes of entry

Session
No

Topics to be covered

Time
(min.)

Historical perspective - discovery of

microscope, Louis Pasteurs contributions I

50

R3 (1-5) &
R4 (6-7)

Historical perspective - discovery of

microscope, Louis Pasteurs contributions - II

50

R3 (1-5) &
R4 (6-7)

Robert Kochs postulates

50

TB4 (19-22)

Early discoveries of microbial toxins Toxic

assays

Reference

Teaching Method
LCD
LCD
LCD
LCD

50

R4 (622-628)
LCD

Early discoveries of vaccines & antibiotics

50

R4 (622-628)

Birth of molecular genetics

50

TB1 (1-10)

Birth of modern molecular pathogenesis

studies

50

TB1 (1-10)

Various pathogen types.

50

R4 (628-700)

Pathogen modes of entry.

50

R4 (628-700)

10

Revision

50

LCD
LCD
LCD
LCD
LCD

DOC/LP/01/28.02.02

LP- BT2023

LESSON PLAN
BT2023
Unit: II

Sub Code & Name:

MOLECULAR PATHOGENESIS

Branch: Biotechnology

Semester : VI

LP Rev. No: 04
Date: 31.12.14
Page 2 of 7

UNIT II: HOST-DEFENSE AGAINST PATHOGENS AND PATHOGENIC STRATEGIES

Attributes & components of microbial pathogenesis, Host defense: skin, mucosa, cilia, secretions,
physical movements, limitation of free iron, antimicrobial compounds, mechanism of killing by
humoral and cellular defense mechanisms, complements, inflammation process, general disease
symptoms, Pathogenic adaptations to overcome the above defenses.
Session
No

Topics to be covered

Time
(min.)

Reference

Teaching Method
LCD

11

Attributes & components of microbial

pathogenesis

50

TB4(34-36) &
TB1(43-61)
LCD

12

Host defense: skin, mucosa, cilia, secretions,

physical movements, limitation of free iron,

50

TB4 (53-66)

13

Antimicrobial compounds

50

TB4 (150-165)

14

Mechanism of killing by humoral defense

mechanisms

50

R5 (389 410)

15

Mechanism of killing by cellular defense

mechanisms

50

R5 (389 410)

16

Complements

50

TB4 (69-73) &

R4 (714- 718)

17

Inflammation process

50

R4 (712- 714)

18

General disease symptoms

50

R4 (850- 858)

19

Pathogenic adaptations to overcome the above

defenses I

50

20

Pathogenic adaptations to overcome the above

defenses

50

LCD

LCD
LCD
LCD
LCD
LCD

TB4 (115-128)
LCD
&
R4 (801-802)
TB4 (115-128)
LCD
&
R4 (801-802)
DOC/LP/01/28.02.02

LP- BT2023

LESSON PLAN
BT2023
Unit: III

Sub Code & Name:

MOLECULAR PATHOGENESIS

Branch: Biotechnology

Semester : VI

LP Rev. No: 04
Date: 31.12.14
Page 3 of 7

UNIT III MOLECULAR PATHOGENESIS (WITH SPECIFIC EXAMPLES)

Virulence, virulence factors, virulence-associated factors and virulence lifestyle factors, molecular genetics
and gene regulation in virulence of pathogens, Vibrio Cholerae: Cholera toxin, co-regulated pili, filamentous
phage, survival E.coli pathogens: Enterotoxigenic E.coli (ETEC), labile & stable toxins, Entero- pathogenic
E.coli (EPEC), type III secretion, cytoskeletal changes, intimate attachment; Enterohaemerrohogic E.coli
(EHEC), mechanism of bloody diarrhoea and Hemolytic Uremic Syndrome, Enteroaggregative E.coli
(EAEC). Shigella: Entry, macrophage apoptosis, induction of macropinocytosis, uptake by epithelial cells,
intracellular spread, inflammatory response, tissue damage Plasmodium: Life cycle, erythrocyte stages,
transport mechanism and processes to support the rapidly growing schizont, parasitiparous vacuoles, and
knob protein transport, Antimalarials based on transport processes. Influenza virus: Intracellular stages,
Neuraminidase & Haemagglutinin in entry, M1 & M2 proteins in assembly and disassembly, action of
amantidine.
Session
No

Topics to be covered

Time
(min.)

Reference

21

Virulence, virulence factors, virulence-associated

factors - I

50

TB1(2-3)

22

Virulence, virulence factors, virulence-associated

factors - II

50

TB1(2-3)

23

Molecular genetics and gene regulation in

virulence of pathogens I

50

TB1(76115)

24

Molecular genetics and gene regulation in

virulence of pathogens - II

50

TB1(76115)

Teaching
Method
LCD
LCD

25

LCD
LCD

50

TB1(457489)

LCD

Vibrio Cholerae: Cholera toxin, co-regulated pili,

filamentous phage
26

Survival of E.coli pathogens: Enterotoxigenic

E.coli (ETEC), labile & stable toxins,

50

TB1(390407)

LCD

27

Entero- pathogenic E.coli (EPEC), type III

secretion, cytoskeletal changes, intimate
attachment

50

TB1(390407)

LCD

UNIT III MOLECULAR PATHOGENESIS (WITH SPECIFIC EXAMPLES) (Cont.)

LESSON PLAN
BT2023
Unit: III

Sub Code & Name:

MOLECULAR PATHOGENESIS

Branch: Biotechnology Semester : VI

LP- BT2023
LP Rev. No: 04
Date: 31.12.14
Page 4 of 7

Sessio

DOC/LP/01/28.02.02

LP- BT2023

LESSON PLAN
BT2023
Unit: IV

LP Rev. No: 04

Sub Code & Name:

MOLECULAR PATHOGENESIS

Branch: Biotechnology

Date: 31.12.14
Page 5 of 7

Semester : VI

UNIT IV: EXPERIMENTAL STUDIES ON HOST-PATHOGEN INTERACTIONS

Virulence assays: adherence, invasion, cytopathic, cytotoxic effects. Criteria & tests in identifying
virulence factors, attenuated mutants, molecular characterization of virulence factors, signal
transduction & host responses

Session
No

Time
(min.)

Topics to be covered

41

Virulence assays

50

42

Adherence, invasion,

50

43

Cytopathic, cytotoxic effects

50

44

Criteria & tests in identifying virulence

factors- Immunological assays

50

45

Criteria & tests in identifying virulence

factors Molecular methods

50

46

Attenuated mutants

50

47

Molecular characterization of virulence

factors

50

48

Signal transduction & host responses

50

Reference
TB4 (24-28)

Teaching Method
LCD
LCD

TB4 (24-28)
LCD

49
50

TB3 (512-522)
LCD
TB3 (113-122)
LCD
TB3 (75-81)
LCD
TB3 (75-81)
LCD

Signal transduction & host responses

50

Revision

50

TB3 (468-483)
LCD
TB3 (468-483)
LCD
TB3 (468-483)

DOC/LP/01/28.02.02

LP- BT2023

LESSON PLAN
BT2023
Unit: V

LP Rev. No: 04

Sub Code & Name:

MOLECULAR PATHOGENESIS

Branch: Biotechnology

Date: 31.12.14

Semester : VI

Page 6 of 7

UNIT V MODERN APPROACHES TO CONTROL PATHOGENS

Classical approaches based on serotyping. Modern diagnosis based on highly conserved virulence
factors, immuno & DNA-based techniques. New therapeutic strategies based on recent findings
on molecular pathogenesis of a variety of pathogens, Vaccines - DNA, subunit and cocktail
vaccines.
Session
No

Topics to be covered

Time
(min.)

Reference

Teaching Method
LCD

51

Classical approaches based on serotyping I

50

TB1 (134-135)

52

Classical approaches based on serotyping II

50

TB1 (134-135)

53

Modern diagnosis based on highly conserved

virulence factors

50

TB1 (165-166)

54

Immuno based techniques

50

TB1(144-146)

55

DNA -based techniques

50

TB1(149-164)

50

TB1 (166-170)
& Websource

50

TB1 (166-170)
& Websource

LCD
LCD

LCD
LCD

56

57

New therapeutic strategies based on

findings on molecular pathogenesis of a
of pathogens I
New therapeutic strategies based on
findings on molecular pathogenesis of a
of pathogens I

recent
variety
recent
variety

58

Vaccines - DNA

50

R5 (425 -427)

59

Vaccines - subunit & cocktail vaccines

50

R5(427 -428)

60

Revision

50

LCD

LCD

LCD
LCD