Professional Documents
Culture Documents
Selectra I
Selectra I
Continuous-spectrophotometric
DEA BUFFER
Instrument:
SELECTRA I
Reagent preparation
Samples
Performance characteristics
Serum, plasma.
Alkaline phosphatase in serum or plasma is stable for 7 days at 2-8C.
Heparin may be used as anticoagulant
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra
Fosfatasa alcalina
FAL
Cintica 10 %
51 seg.
405 nm
U/l
0
5
ul
2
ul
250 ul
253 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
0.000 Abs.
0.600 Abs.
Si
No
Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
2764.00
90 U/l
280 U/l
90
U/l
280 U/l
-
37 C
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Alanine aminotransferase (ALT or GPT) catalyzes the transfer of the amino Working Reagent: Pour the contents of the Reagent B into the Reagent A
group from alanine to 2-oxoglutarate, forming pyruvate and glutamate. The bottle. Mix gently.
catalytic concentration is determined from the rate of decrease of NADH, Stable for 2 months at 2-8C.
measured at 340 nm, by means of the lactate dehydrogenase (LDH) coupled
reaction.
Samples
Performance characteristics
Serum.
Alanine aminotransferase in serum is stable for 7 days at 2-8C.
Interferences: High pyruvate in the sample will consume NADH during the
delay time before measurements, reducing the linearity of the method.
Linearity: Up to 500 U/L.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
ALT/GPT
ALT
Cintica 10 %
70 seg.
340 nm
U/l
0
23
ul
5
ul
230 ul
248 ul
0.600 Abs.
3.000 Abs.
3.000 Abs.
1.000 Abs.
3.000 Abs.
Si
No
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
-1746.0
2 U/l
41 U/l
2 U/l
41 U/l
-
37 C
-AMYLASE
Continuous-spectrophotometric
DIRECT SUBSTRATE
Instrument:
SELECTRA I
Reagent preparation
Samples
Performance characteristics
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra
AMILASA
AMY
Cintica 10 %
32 seg.
405 nm
U/l
0
5
ul
2
ul
250 ul
253 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
-0.100 Abs.
0.500 Abs.
Si
No
Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
3292.00
0
U/l
60 U/l
0
U/l
60 U/l
-
37 C
CALCIUM
Spectrophotometric
METHYLTHYMOL BLUE
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Calcium in the sample reacts with methylthymol blue in alkaline medium Working Reagent: Mix equal volumes of Reagent A and Reagent B. Mix
forming a coloured complex that can be measured by spectrophotometry. thoroughly.
Hydroxyquinoline is included in the reagent to remove magnesium interference. Stable for 2 days at 2-8C.
Samples
Performance characteristics
Linearity: Up to 15 mg/dL.
Interferences: Hemoglobin (1.5 g/L), bilirubin (20 mg/dL), magnesium (10
mg/dL) and phosphate (20 mg/dL) do not interfere.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
CALCIO
CAL
Punto final
11.5 min
620 nm
mg/dl
1
3
ul
2
ul
300 ul
301 ul
4.000 mg/dl.
15.000 mg/dl.
-0.100 Abs.
0.600 Abs.
Si
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
CALCIO
3
1
*
*
*
**
9.0 mg/dl
10.7 mg/dl
9.0 mg/dl
10.7 mg/dl
-
37 C
CHOLESTEROL
Enzymatic-spectrophotometric
CHOLESTEROL OXIDASE/PEROXIDASE
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Free and esterified cholesterol in the sample originates, by means of some Reagent is ready to be used
coupled reactions, a coloured complex that can be measured by
spectrophotometry.
Samples
Performance characteristics
Serum or plasma.
Stable for 7 days at 2-8C.
Heparin, EDTA, oxalate and fluoride may be used as anticoagulants.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. Infer.
Conc. Super.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra
COLESTEROL
COL
Punto final
11.5 min
505 nm
mg/dl
0
3
ul
2
ul
250 ul
251 ul
0.000 mg/dl.
500.00 mg/dl.
-0.100 Abs.
0.300 Abs.
Si
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
COLESTEROL
3
1
*
*
*
**
123 mg/dl
270 mg/dl
123 mg/dl
243 mg/dl
-
37 C
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Creatine kinase (CK) catalyzes the phosphorylation of ADP, in the presence of Working Reagent: Empty the contents of a Reagent B vial into a Reagent A
creatine phosphate, to form ATP and creatine. The catalytic concentration is bottle. Swirl gently.
determined from the rate of NADPH formation, measured at 340 nm, by means Stable for 15 days at 2-8C.
of the hexokinase (HK) and glucose-6-phosphate coupled reactions.
Samples
Performance characteristics
Serum.
Creatine kinase in serum is stable for 7 days at 2-8C.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
CK-NAC
CK
Cintica 10 %
129 seg.
340 nm
U/l
0
10
ul
2
ul
250 ul
258 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
-0.100 Abs.
0.800 Abs.
Si
No
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
4127.00
24 U/l
195 U/l
24 U/l
170 U/l
-
37 C
CREATININE
Kinetic-spectrophotometric
ALKALINE PICRATE
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Creatinine in the sample reacts with picrate in alkaline medium forming a Working Reagent: Mix equal volumes of Reagent A and Reagent B. Mix
coloured complex. The complex formation rate is measured in a short period to thoroughly.
avoid interferences.
Stable for 2 months at 2-8C.
Samples
Performance characteristics
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
1er punto
2do punto
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Desv. Abs. React
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
CREATININA
CRE
Dos puntos
32 seg
90 seg
505 nm
mg/dl
2
25
ul
5
ul
250 ul
270 ul
0.300 mg/dl.
20.000 mg/dl.
3.000 Abs
-0.100 Abs.
0.500 Abs.
Si
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
CREATININA
3
1
*
*
*
**
0.60 mg/dl
1.10 mg/dl
0.60 mg/dl
1.10 mg/dl
-
37 C
PHOSPHORUS
Spectrophotometric
PHOSPHOMOLYBDATE/UV
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Inorganic phosphorus in the sample reacts with molybdate in acid medium Working Reagent: Mix 35 mL Reagent A + 15 mL Reagent B. Mix thoroughly.
forming a phosphomolybdate complex that can be measured by Stable for 12 months at 15-30C.
spectrophotometry.
Samples
Performance characteristics
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. Infer.
Conc. Super.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra
FSFORO
FOS
Punto final
11.5 min
340 nm
mg/dl
1
3
ul
2
ul
300 ul
301 ul
0.500 mg/dl.
20.000 mg/dl.
-0.100 Abs.
0.500 Abs.
Si
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
FOSFORO
3
1
*
*
*
**
2.7 mg/dl
4.5 mg/dl
2.7 mg/dl
4.5 mg/dl
-
37 C
FRUCTOSAMINE
Kinetic-spectrophotometric
NBT
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Serum glycated proteins reduce nitroblue tetrazolium (NBT) salts in alakaline Reagent is ready to be used.
medium. The rate of formazan at a given temperature is proportional to the
serum glycated proteins
Samples
Performance characteristics
Serum.
Stable for 1 week at 2-8C and for 2 months at 20C.
Hemolysed samples are not suitable for testing.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
1er punto
2do punto
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Desv. Abs. React
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
FRUCTOSAMINA
FRUC
Dos Puntos
285 seg
422 seg
546 nm
mol/l
0
30 ul
15 ul
250 ul
265 ul
0.000 mol/l
7.000 mol/l
3.000 Abs
-0.100 Abs
0.800 Abs
SI (-0.000)
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
FRUCT BIO
3
1
*
*
*
**
0 mol/l
285 mol/l
0 mol/l
285 mol/l
0
0
37 C
GAMMA-GLUTAMYLTRANSFERASE (-GT)
Continuous-spectrophotometric
IFCC
Instrument:
SELECTRA I
Reagent preparation
Samples
Performance characteristics
Serum.
Gamma-glutamyltransferase in serum is stable for 5 days at 2-8C.
Gamma-glutamyltransferase (-GT) catalyzes the transfer of the -glutamyl Working Reagent: Reconstitute the contents of a Reagent B vial with 15 mL (if
group from -glutamyl-3-carboxy-4-nitroanilide to glycylglycine, liberating 3- 10x15 mL size) or 50 mL (if 4x50 mL size) of Reagent A. Swirl gently.
carboxy-4-nitroaniline. The catalytic concentration is determined from the rate Stable for 2 months at 2-8C.
of 3-carboxy-4-nitroaniline formation.
Linearity: up to 300 U/L.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
GGTP
GGT
Cintica 10 %
32 seg.
405 nm
U/l
0
25
ul
5
ul
250 ul
270 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
-0.100 Abs.
1.000 Abs.
Si
No
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
1111.00
15 U/l
60 U/l
10 U/l
40 U/l
-
37 C
GLUCOSE
Enzymatic-spectrophotometric
GLUCOSE OXIDASE/PEROXIDASE
Instrument:
SELECTRA I
Reagent preparation
Glucose in the sample originates, by means of some coupled reactions, a Reagent is ready to be used
coloured complex that can be measured by spectrophotometry.
Samples
Performance characteristics
Serum or plasma.
Stable for 7 days at 2-8C.
Heparin, EDTA, oxalate and fluoride may be used as anticoagulants.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
GLUCOSA
GLU
Punto final
11.5 min
505 nm
mg/dl
0
2 L
300 L
301 L
10.000 mg/dl.
500.000 mg/dl.
-0.100 Abs.
0.300 Abs.
Si
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. Hombre inf.
*
*
*
*
*
*
**
70 mg/dl
110 mg/dl
70 mg/dl
110 mg/dl
37 C
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino Working Reagent: Pour the contents of the Reagent B into the Reagent A
group from aspartate to 2-oxoglutarate, forming oxalacetate and glutamate. bottle. Mix gently.
The catalytic concentration is determined from the rate of decrease of NADH, Stable for 2 months at 2-8C.
measured at 340 nm, by means of the malate dehydrogenase (MDH) coupled
reaction.
Samples
Performance characteristics
Serum.
Aspartate aminotransferase in serum is stable for 7 days at 2-8C.
Interferences: High pyruvate in the sample will consume NADH during the
delay time before measurements, reducing the linearity of the method.
Linearity: Up to 500 U/L.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
AST/GOT
AST
Cintica 10 %
70 seg.
340 nm
U/l
0
23
ul
5
ul
230 ul
248 ul
0.600 Abs.
3.000 Abs.
3.000 Abs.
1.000 Abs.
3.000 Abs.
Si
No
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
-1746.0
2 U/l
42 U/l
2 U/l
42 U/l
-
37 C
IRON
Spectrophotometric
FERROZINE
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Transferrin-bound ferric ions in the sample are released by guanidinium and Working Reagent: Transfer the contents of one Reagent B vial into a Reagent
reduced to ferrous by means of hydroxylamine. Ferrous ions react with A bottle. Mix thoroughly.
ferrozine forming a coloured complex that can be measured by Stable for 6 months at 2-8C.
spectrophotometry.
Samples
Performance characteristics
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Absorb. super.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
HIERRO
FE
Punto final
11.5 min
578 nm
ug/dl
0
30
ul
15
ul
250 ul
265 ul
0.000 ug/dl.
1.000 ug/dl.
-0.100 Abs.
0.300 Abs.
Si
SI
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
HIERRO
3
1
*
*
*
**
70 U/l
155 U/l
55 U/l
140 U/l
-
37 C
Instrument:
SELECTRA I
Reagent preparation
Lactate dehydrognase (LD or LDH) catalyzes the reduction of pyruvate by Working Reagent: Reconstitute the contents of a Reagent B vial with 3 mL (if
NADH, to form lactate and NAD+. The catalytic concentration is determined 20x3 mL size) or 15 mL (if 10x15 mL size)of Reagent A. Swirl gently.
from the rate of decrease of NADH, measured at 340 nm.
Stable for 2 months at 2-8C.
Samples
Performance characteristics
Serum or plasma.
Lactate dehydrogenase in serum or plasma is stable for 24 hours at 2-8C.
Heparin may be used as anticoagulant.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
LDH
LDH
Cintica 10 %
32 seg
340 nm
U/l
0
5
ul
2
ul
250 ul
253 ul
0.400 Abs
3.000 Abs.
3.000 Abs.
1.000 Abs.
2.000 Abs.
Si
No
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
0
TEMPERATURA 37 C
-8095.00
207 U/l
414 U/l
207 U/l
414 U/l
-
37 C
to
the
high
lactate
MAGNESIUM
Spectrophotometric
CALMAGITE
Instrument:
SELECTRA I
Reagent preparation
Magnesium in the sample reacts with calmagite in alkaline medium forming a Reagent is ready to be used.
coloured complex that can be measured by spectrophotometry. EGTA is
included in the reagent to remove calcium interference.
Samples
Performance characteristics
Interferences: Hemoglobin (1.5 g/L), calcium (20 mg/dL) and bilirubin (20
mg/dL) do not interfere.
Linearity: Up to 4 mg/dL.
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
MAGNESIO
MAG
Punto final
11.5 min
546 nm
mg/dl
1
3
ul
2
ul
300 ul
301 ul
0.500 mg/dl.
4.000 mg/dl.
-0.100 Abs.
0.900 Abs.
Si
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
MAGNESIO
3
1
*
*
*
**
1.8 mg/dl
2.1 mg/dl
1.8 mg/dl
2.1 mg/dl
-
37 C
PROTEIN
Spectrophotometric
BIURET
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Protein in the sample reacts with copper (II) ion in alkaline medium forming a Reagent is ready to be used.
coloured complex that can be measured by spectrophotometry.
Samples
Performance characteristics
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. Rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Lm. infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra
PROTEINAS
PRO
Punto Final
11.5 min
546 nm
g/dl
1
5 L
3 L
250 L
252 L
0.000 g/dl
10.000 g/dl
-0.100 Abs
0.500 Abs
SI
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
*
*
*
*
*
*
**
6.0 g/dl
8.0 g/dl
6.0 g/dl
8.0 g/dl
37 C
UREA/BUN
Enzymatic-spectrophotometric
ULTRAVIOLET
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Urea in the sample consumes, by means of some coupled reactions, NADH Working Reagent: Transfer the contents of one Reagent B vial into a Reagent
that can be measured by spectrophotometry.
A bottle. Mix thoroughly.
Stable for 2 months at 2-8C.
Samples
Performance characteristics
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
1er punto
2o punto
Long. Onda
Unidades
Decimales
Vol. Muestra
Vol. Muest. rerun
Vol. Reactivo
Vol. React. rerun
Conc. Infer.
Conc. Super.
Desv.abs.react.
Lm. Infer. abs. react.
Lm. Super. abs. react.
Blanco reactivo
Blanco muestra
UREA
URE
Dos puntos
51 seg
110 seg
340 nm
mg/dl
0
3
ul
2
ul
399 ul
400 ul
5.000 mg/dl.
250.00 mg/dl.
3.000 Abs
1.250 Abs.
3.000 Abs.
Si
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. Inferior
Lm. Superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
UREA
3
1
*
*
*
**
10 mg/dl
50 mg/dl
10 mg/dl
50 mg/dl
-
37 C
URIC ACID
Enzymatic-spectrophotometric
URICASE/PEROXIDASE
Instrument:
Principle of the method
SELECTRA I
Reagent preparation
Uric acid in the sample originates, by means of some coupled reactions, a Reagent is ready to be used.
coloured complex that can be measured by spectrophotometry.
Samples
Performance characteristics
Instrument settings
Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Conc. infer.
Conc. super.
Lm. infer. abs. react.
Lm. super. abs. react.
Blanco reactivo
Blanco muestra
ACIDO URICO
URI
Punto final
11.5 min
546 nm
mg/dl
1
5
ul
3
ul
250 ul
252 ul
0.000 mg/dl.
25.000 mg/dl.
-0.100 Abs.
0.300 Abs.
Si
NO
Calibrador
Repetir
Nmero
Concentracin
Lm. inferior
Lm. superior
Factor
Ref. hombre inf.
Ref. hombre sup.
Ref. mujer inf.
Ref. mujer sup.
Ref. ped. inf.
Ref. ped sup.
Control 1
Control 2
Control 3
TEMPERATURA
AC.URICO
3
1
*
*
*
**
3.4 mg/dl
7.0 mg/dl
2.4 mg/dl
5.7 mg/dl
-
37 C