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Electrophoresis Ge - Introduction: Lecture Notes - Handouts 10/3/2013
Electrophoresis Ge - Introduction: Lecture Notes - Handouts 10/3/2013
10/3/2013
ELECTROPHORESIS
GE - INTRODUCTION
What is Electrophoresis?
Electrophoresis is the study of the movement of
charged molecules in an electric field.
Electrophoresis
is
a
basic
biotechnological
technique/process in which charged biomolecules are
separated according to their mass or charge by the use
of an electric field.
What is Gel Electrophoresis?
Gel electrophoresis involves use of a gel or gelatinous
material which is electrically transparent and acts as a
porous sieve and thus forming a suitable support
medium for electrophoresis of charged molecules.
What are the applications of GE?
GE is used generally for the separation of dyes, proteins
and nucleic acids
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ELECTROPHORESIS - CLASSIFICATION
Capillary Electrophoresis
- FSCE/CZE
- CGE
- CIEF
- CETP
- ACE
- IACE
- NCE
Slab Electrophoresis
- PE
- GE
- SAGE, etc
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GE - CLASSIFICATION
CE vs GE
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Principle of CE
Samples are introduced into the capillary for separation by two different
methods. Both having advantages and disadvantages; Electrokinetic
injection and Hydrodynamic (Vacuum or Pressure) Injection.
GE - Works according to
electrophoretic moment and thus
yielding less resolution than CE.
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SDS PAGE
GE General Procedure
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GE - Instrumentation
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GE - Instrumentation
Vertical Gel Electrophoresis Assembly
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GE - Instrumentation
GE General Procedure
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