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Journal of Biodiversity and Environmental Sciences (JBES)
Journal of Biodiversity and Environmental Sciences (JBES)
Journal of Biodiversity and Environmental Sciences (JBES)
2015
Journal of Biodiversity and Environmental Sciences (JBES)
ISSN: 2220-6663 (Print), 2222-3045 (Online)
http://www.innspub.net
Vol. 6, No. 4, p. 115-123, 2015
RESEARCH PAPER
OPEN ACCESS
recent
decades
many
new
non-cyano-substituted
and
cyano
substituted
unicellular
sensitive to such
of
this
contaminant
at
different
oxygen
Test organisms
species
(ROS)
are
generated.
Studies
Test chemical
Cyfluthrin,
The
deltamethrin
and
cypermethrin
in
insecticide
used
for
our
experiments
is
Treatment
g/l.
proteins.
The
GPx
activity
was
determined
Lipid estimation
Lipids are determined by the method described by
min/ mg of proteins.
Polarographic study
measured
using
an
oxygen
electrode
type
insecticide concentrations.
difference
(p
0.01)
for
Statistical analysis
control.
the
two
highest
compared to the
Results
Determination of IC50
The IC50 was calculated from the linear equation of y
= 16.32 - 17.20 (Fig. 2). The estimated 96h IC50
value
for
Paramecium
tetraurelia
exposed
to
2.
Effect
of cypermethrin treatment
on
Paramecium
tetraurelia
exposed
to
Lipid estimation
Fig. 3 represents the effect of cypermethrin treatment
on the rate of total lipids in Paramecium tetraurelia.
We observed an important dose-dependent decrease
increasing
concentrations
of
cypermethrin
are
an
important
dose-dependent
decrease
MDA
Control
0.05
0.3930.02
0.46014
0.6300.07
0.9650.14
ns
**
7.2770.38
6.7180.72
5.0530.74
ns
ns
8.3850.18
8.7890.50
13.7070.49
(mol/mg Proteins)
ns
GSH
7.3411.97
(mol/mg Proteins)
ns
GPx
6.8750.20
10.0100.77
(mol/min/mg Proteins)
0.5
**
**
12.9461.32
16.3730.66
18.6580.66
***
**
**
1.0580.34
3.2470.23
18.6660.37
20.9420.66
Values are the mean of three essays per treatment with the corresponding standard error .ns, *, **, *** indicate,
respectively, statically
statically high significant differences (p 0.01) and statically very high significant differences (p 0.001) relative
to the control.
GPx activity was significantly increased in cells
g/l,
represented in Fig. 4.
respectively.
Statistical
study
showed
significant
(P
0.01)
for
the
two
highest
in
different
concentrations
dose-dependent
manner
at
toxicants
of
is involved in the
pesticides
(Bifenazate
and
machinery.
have
zebrafish
prawn
intoxicated
Palaemon
with
serratus
different
doses
intoxicated
of
with
respiratory
by
Paramecium
metabolism
cypermethrin
to
the
disturbance
protozoan
caused
result
tetraurelia.
in cellular
membrane.
Bioavailability
of
cypermethrin
in
the
exposed to -
2011).
Furthermore,
the
free
radicals
generated
by
(Paital, 2012)
such as GPx.
LDH
activity
was
significantly
increased
in
peroxidation,
oxidative
stress
and
respiratory
Comprehension
sublethal
management.
metabolism.
of
effects
the
by
its
Furthermore,
mechanisms
caused
altered
related
chemicals
to
upon
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