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TSWV
TSWV
TSWV
610
Fig. 1. Percentage of plants of peanut cultivars GK7, NC7, VC1, Florunner (FR), Southern Runner (SR), and Spanco exhibiting visual symptoms over
time following mechanical inoculation with tomato spotted wilt virus (TSWV), peanut mottle virus (PMV), or a mixture of both viruses (TSWV/ PMV).
Plant Disease / June 1998
611
Fig. 2. Systemically infected leaves of the peanut cultivar GK7, 14 days after inoculation with peanut
mottle virus (A) and tomato spotted wilt virus (B), showing similar symptoms, and 14 days after inoculation with a mixture of the two viruses (C and D), indicating a wide range of symptom types and
severity.
612
Table 1. Tomato spotted wilt virus (TSWV) antigen titer in peanut plants infected with TSWV or
with TSWV and peanut mottle virus (PMV), as determined by enzyme-linked immunosorbent assay
(ELISA)
Days after
inoculationa
8
13
19
26
33
40
47
ELISA valuesb
TSWV
TSWV+ PMVc
Standard
error
Treatment
difference
0.014
0.083
0.268
0.284
0.222
0.098
0.115
0.017
0.017
0.064
0.252
0.183
0.114
0.099
0.001
0.008
0.014
0.017
0.013
0.012
0.011
P 0.05
P 0.05
P 0.05
d
P 0.001
The youngest quadrifoliate leaf was assayed at the times indicated. Leaf extracts were prepared at
1:100 (wt / vol) in carbonate coating buffer (pH 9.6).
b Optical density (A
405 nm), mean value of 72 plants. No cultivar treatment interaction occurred.
Therefore, comparisons of treatments were made with data averaged over the cultivars. Optical
density values of the uninoculated control ranged between 0.01 and 0.02.
c Inocula of the viruses were mixed 1:1 prior to inoculation.
d Not significant.
Table 2. Peanut mottle virus (PMV) antigen titer in peanut plants infected with PMV or with PMV
and tomato spotted wilt virus (TSWV), as determined by enzyme-linked imunosorbent assay (ELISA)
ELISA valuesb
Days after
inoculationa
PMV
PMV+ TSWVc
Standard
error
Treatment
difference
8
13
19
26
33
40
47
0.777
1.847
1.158
0.288
0.672
0.611
0.255
0.385
1.498
1.373
0.331
0.882
0.551
0.303
0.058
0.044
0.084
0.037
0.048
0.049
0.032
P 0.05
P 0.05
d
P 0.001
The youngest quadrifoliate leaf was assayed at the times indicated. Leaf extracts were prepared at
1:100 (wt / vol) in carbonate coating buffer (pH 9.6).
b Optical density (A
405 nm), mean value of 72 plants. No cultivar treatment interaction occurred.
Therefore, comparisons of treatments were made with data averaged over the cultivars. Optical
density values of the uninoculated control ranged between 0.01 and 0.03.
c Inocula of the viruses were mixed 1:1 prior to inoculation.
d Not significant.
ACKNOWLEDGMENTS
This project was supported in part by a grant
through a cooperative agreement with the University of Georgia supported by Goldkist.
LITERATURE CITED
1. Anderson, E. J., Kline, A. S., Morelock, T. E.,
and McNew, R. W. 1996. Tolerance to blackeye cowpea mosaic potyvirus not correlated
with decreased virus accumulation or protection from cowpea stunt disease. Plant Dis. 80:
847-852.
2. Anjos, J. R., Jarlfors, U., and Ghabrial, S. A.
1992. Soybean mosaic potyvirus enhances the
titer of two comoviruses in dually infected soybean plants. Phytopathology 82:1022-1027.
3. Bijaisoradat, M., Kuhn, C. W., and Benner,
C. P. 1988. Disease reactions, resistance, and
viral antigen content in six legume species infected with eight isolates of peanut mottle virus. Plant Dis. 72:1042-1046.
4. Black, M. C. 1991. Effects of spotted wilt on
selected peanut cultivars. Proc. Am. Peanut
Res. Educ. Soc. 23:52.
5. Black, M. C., Lummus, P. F., Smith, D. H., and
Demski, J. W. 1986. An epidemic of spotted
wilt disease in South Texas peanuts in 1985.
Proc. Am. Peanut Res. Educ. Soc. 18:58.
6. Black, M. C., Tewolde, H., Fernandez, C. F.,
and Schubert, A. M. 1994. Effects of seeding
rate, irrigation, and cultivar on spotted wilt,
rust, and southern blight diseases of peanut.
Proc. Am. Peanut Res. Educ. Soc. 26:50.
7. Bock, K. R., and Kuhn, C. W. 1975. Peanut
mottle virus. No. 141 in: Descriptions of Plant
Viruses. Commonw. Mycol. Inst. /Assoc. Appl.
Biol., Kew, England.
8. Bowman Vance, V. 1991. Replication of potato virus X is altered in coinfections with potato virus Y. Virology 182:486-494.
9. Bowman Vance, V., Berger, P. H., Carrington,
J. C., Hunt, A. G., and Shi, X.-M. 1995. 5
Proximal potyviral sequences mediate potato
virus X / potyviral synergistic disease in transgenic tobacco. Virology 206:583-590.
10. Brown, S. L., Todd, J. W., and Culbreath,
A. K. 1996. Effect of selected cultural practices on incidence of tomato spotted wilt virus
and populations of thrips vectors in peanuts.
Acta Hortic. 431:491-498.
11. Brunt, A. A., Cabtree, K., and Gibbs, A. 1990.
Viruses of Tropical Plants. C.A.B. International, Wallingford, England.
12. Calvert, L. A., and Ghabrial, S. A. 1983. Enhancement by soybean mosaic virus of bean
pod mottle virus titer in doubly infected soybean. Phytopathology 73:992-997.
13. Culbreath, A. K., Csinos, A. S., Brenneman,
T. B., Demski, J. W., and Todd, J. W. 1991.
Association of tomato spotted wilt virus with
foliar chlorosis of peanut in Georgia. Plant
Dis. 75:863.
14. Culbreath, A. K., Todd, J. W., Branch, W. D.,
Brown, S. L., Demski, J. W., and Beasley, J. P.,
Jr. 1994. Effect of new peanut cultivar Georgia
Browne on epidemics of spotted wilt. Plant
Dis. 78:1185-1189.
15. Culbreath, A. K., Todd, J. W., Demski, J. W.,
and Chamberlin, J. R. 1992. Disease progress
of spotted wilt in peanut cultivars Florunner
and Southern Runner. Phytopathology 82:766771.
16. Culbreath, A. K., Todd, J. W., Gorbet, D. W.,
Branch, D. W., Holbrook, C. C., Shokes, F. M.,
and Demski, J. W. 1996. Variation in susceptibility to tomato spotted wilt virus among
advanced breeding lines of peanut (Arachis
hypogaea). Acta Hortic. 431:402-410.
17. Darmirdagh, I. S., and Ross, A. F. 1967. A
marked synergistic interaction of potato virus
X and Y in inoculated leaves of tobacco. Virology 31:296-307.
18. Demski, J. W., Alexander, A. T., Stefani, M. A.,
613
19.
20.
21.
22.
23.
24.
25.
26.
27.
614
and Kuhn, C. W. 1983. Natural infection, disease reactions, and epidemiological implications of peanut mottle virus in cowpea. Plant
Dis. 67:267-269.
Demski, J. W., Smith, D. H., and Kuhn, C. W.
1975. Incidence and distribution of peanut
mottle virus in peanut in the United States.
Peanut Sci. 2:91-93.
Dwivedi, S. L., Nigam, S. N., Reddy, D. V. R.,
Ranga Rao, G. V., and Reddy, A. S. 1996.
Registration of ICGV 86388 peanut germplasm. Crop Sci. 36:1423.
Goldberg, K.-B., and Brakke, M. K. 1987.
Concentration of maize chlorotic mottle virus
increased in mixed infections with maize
dwarf mosaic virus, strain B. Phytopathology
77:162-167.
Goodman, R. M., and Ross, A. F. 1974. Enhancement of potato virus X synthesis in
doubly infected tobacco occurs in doubly infected cells. Virology 58:16-24.
Gorbet, D. W., and Shokes, F. M. 1994. Plant
spacing and tomato spotted wilt virus. Proc.
Am. Peanut Res. Educ. Soc. 26:50.
Gudauskas, R. T., Burch, K. B., Jin, P., Hagan,
A. K., and Weeks, J. R. 1993. Identification of
viruses infecting peanut in Alabama. Peanut
Sci. 20:71-73.
Hagan, A. K., Weeks, J. R., French, J. C., Gudauskas, R. T., Mullen, J. M., Gazaway, W. S.,
and Shelby, R. 1990. Tomato spotted wilt virus in peanut in Alabama. Plant Dis. 74:615
Hagan, A. K., Weeks, J. R., Gudauskas, R. T.,
and French, J. C. 1991. Development of control recommendations for TSWV in peanut in
Alabama. Proc. Am. Peanut Res. Educ. Soc.
23:52.
Hagan, A. K., Weeks, J. R., Gudauskas, R. T.,
Gazaway, W. S., and Shelby, R. 1987. Tomato
spotted wilt on peanuts in Alabama. Proc. Am.