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Lab 4 Microbial Metabolism
Lab 4 Microbial Metabolism
Lab 4 Microbial Metabolism
1. CARBOHYDRATE CATABOLISM
2. FERMENTATION OF CARBOHYDRATES
3. PROTEIN CATABOLISM
4. RESPIRATION
5. RAPID IDENTIFICATION METHODS
MICROBIAL METABOLISM
an organism.
Catabolism is the energy-releasing processes.
Anabolism is the energy-using processes.
MICROBIAL METABOLISM
MICROBIAL METABOLISM
1. CARBOHYDRATE CATABOLISM
Most bacteria catabolize carbohydrates for carbon
and energy.
Carbohydrates can be classified based on size:
monosaccharides, oligosaccharides, polysaccharides.
Exzoezymes are mainly hdyrolytic enzymes that
1. CARBOHYDRATE CATABOLISM
Amylase hydrolyzes the polysaccharide starch into
smaller carbohydrates.
In the laboratory, the presence of an exzoenzyme is
Amylase
H2O
glucose+glucose+
1. CARBOHYDRATE CATABOLISM
Procedure of Starch Hydrolysis Test
2. FERMANTATION OF CARBOHYDRATES
Releases energy from fermentation of organic molecules
oxygen.
The metabolic end-products of fermantation are small
organic molecules, usually organic acids. Some bacteria
produce gases from the fermantation of carbohydrates.
2. FERMANTATION OF CARBOHYDRATES
2. FERMANTATION OF CARBOHYDRATES
urease
H2O
hydrolysis-Some
bacteria
split
tryptophan into indole and pyruvic acid using the
hydrolase called tryptophanase.
Indole can be detected with Kovac's reagent (Indole
reagent).
4. Respiration
Catalase Test
Microorganisms able to live in oxygenated environments
4. Respiration
Oxidase Test
The oxidase test is a test
used in microbiology to
determine if a
bacterium produces certain
cytochrome c oxidases. It
uses disks impregnated with
a reagent.
inoculation.
Biolog
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