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Daunting Quest For Stents
Daunting Quest For Stents
Daunting Quest For Stents
10.1586/17434440.2.6.647
647
vascular grafts were developed, and continue to be used hydrostatic and electrostatic [4]. Unfortunately, this overall
clinically with great success. The first, and most satisfactory concept has yet to be embraced by the surgical community.
in terms of mechanical strength and thromboresistance, of It should be noted, however, that the basic technology still
these two compositions is expanded poly(tetrafluoro- holds promise in that it has been shown to reduce, or at least
ethylene) (ePTFE). The second is poly(ethylene terephtha- slow the failure rate of the synthetic polymer grafts, primalate) (PET), better known as Dacron. Unfortunately, the rily ePTFE, in human trials. While the technology is far
patency rates for small diameter (<5 mm ID) vascular pros- from perfect, this most basic form of vascular tissue
theses configured from these materials are clinically engineering imparting some degree of biologic activity to
unacceptable due to acute thrombus formation and chronic the luminal surface of a synthetic construct could potenanastomotic (excessive tissue formation in adjacent arterial tially be, at a minimum, the bridge technology to the next
tissue) and/or intimal (excessive tissue ingrowth through the generation of vascular prosthesis. One major concern that
graft wall) hyperplasia. Thrombogenicity is inherent to the remains, especially when utilizing ePTFE grafts due to their
surface characteristics of the implanted graft materials that nonstick surface characteristics, is the potential for delamilack a functional intima, that is, endothelial cells (ECs). nation of the EC lining, neointima, upon implantation due
Thus, this is a critical aspect to be considered when engi- to surgical, mechanical manipulation and subsequent exponeering the next generation of biomaterials for use as a vas- sure to blood shear stress. It is also speculated that there may
cular graft. Intimal hyperplasia is theorized to initiate via a be a potential for spontaneous and catastrophic delaminacombination of compliance mismatch (mechanical proper- tion of the neointima at anytime after implantation. Besides
ties of the graft differing significantly from those of the adja- the labor- and cost-intensive procedure to implement this
cent native vessel) and an abnornmal cellular micro- method, another major limitation of the seeding procedures
environment (vascular smooth muscle cells interfacing is that many of them require a substantial cell culture period
to allow for cell adhesion and
continuously/permanently with a syn...is it possible to engineer a neointima formation, which introthetic material and not the typical celgraft structure from a
duces concerns regarding cellular
lular microenvironment of neighborand/or
phenotypic
ing cells and extracellular matrix nonbiodegradable polymer to genotypic
[ECM]). One additional concern of meet the stringent mechanical changes, and a risk of infection (cell
culture contamination) [4]. Surgeons
these nondegradable polymeric mateproperties, mechanical
rials during the long-term implantadurability and biocompatibility do not want any additional steps in
tion is the continuous potential for
the operating room they want to
required?
catastrophic infections. Thus, one is
take the graft off-the-shelf and
left wondering, is it possible to engineer a graft structure implant it. During surgery any additional steps, for example,
from a nonbiodegradable polymer to meet the stringent harvesting ECs or waiting for completion of the procedure
mechanical properties, mechanical durability and bio- (because it can be time consuming, often requiring multiple
compatibility required? While probably not impossible, it procedures on different days), would be burdensome. The
seems unlikely that this can be achieved without the use of a take-home message is, keep it simple. Despite these drawsynthetic polymer structure combined with biologic compo- backs, Peter Zilla (Cape Town, South Africa) continues to
nents, including EC seeding/sodding or tissue-engineered champion EC seeding as a clinical tool with an ongoing
constructs, in order to more closely match both the cellular multicenter clinical trial.
environment and compliance of native vasculature.
Over the years, several groups have proposed different
In an attempt to overcome the inherent limitations of approaches which alter the inner surface layer of the vascular
synthetic polymers, Malcolm Herring in the late 1970s prosthesis either physically, chemically or by a drug-eluting
pioneered the technology of EC seeding, followed by others coating which would prevent thrombogenicity [58]. The
with the concept of EC sodding, with the overall hypothesis stent industry has developed, as coatings on metallic stents,
that a small diameter synthetic vascular graft could be made several slow-releasing drugs, which also have potential applimore biocompatible by creating or transplanting a disguise cation on synthetic vascular prostheses. The first trials used
derived from ones own natural vascular luminal lining heparin coating on the luminal surfaces, and showed an
(ECs) [2,3]. Why EC seeding/sodding? The endothelium, improvement in patency, as well as a reduction of neointimal
once thought of as a simple cell lining, in actuality represents hyperplasia in experimental models. However, clinically,
a complex biofactory that produces components critical to these results could not be confirmed, potentially due to the
controlling or inhibiting the failure modes exhibited by the rapid release of the heparin coating. Additionally, immunosynthetic vascular grafts. Since inception of the concept of suppressive agents such as rapamycin or cytostatic drugs
EC seeding, numerous methodologies for EC seeding/sod- have shown excellent results with regard to limiting neoding have been developed. These techniques can be differen- intimal proliferation and restenosis in experimental models
tiated and categorized based upon the unique physical force and large clinical trials. Similar compounds have been used
utilized in each seeding/sodding process: gravitational, for the coating of ePTFE grafts in experimental settings and
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showed some reduction of intimal hyperplasia. However, vascular grafts capable of in situ regeneration [1012]. One
before clinical application, the adverse effects of such potent major advantage of this acellular, in situ regeneration
immunosuppressive drugs and the incidence of thrombus approach is that it removes all concerns regarding cell
formation have to be clarified. More recently, attempts have sourcing for development, as well as cellular genotypic
been made to repopulate the inner surface of implanted and/or phenotypic changes and a risk of infection (cell
vascular grafts with endothelial or progenitor cells. For this culture contamination) during cell/tissue culture. We can
purpose, the grafts were incubated with growth factors or then address the question, How much in vitro fabrication, if
anti-CD34 antibody.
any, is really required beyond scaffolding? Aside from fabricaBioresorbable vascular grafts are analogs to the biostable, tion technique, the bioresorbable graft will have comparable
synthetic polymers, with one major exception. The structural concerns regarding storage, distribution and off-the-shelf
materials utilized in the design of the graft are capable of availability to the current biostable grafts.
acting as a vascular graft, while at the same time promoting
While EC seeding is considered by many to be the first
arterial regeneration in situ. The challenge in these designs is and simplest form of vascular tissue engineering, it does not
coordinating the time of degradation (loss of mechanical form a neoartery. More complex attempts have been
strength) with the time of significant tissue regeneration conducted by combining synthetics (biostable and bio(which must take up the loss of strength accordingly) in order degradable) with natural materials and cells, to incorporate a
to prevent catastrophic failure (aneurysm formation/rupture). higher degree of biologic activity, thereby reducing the
Substantial work has been performed over the past 40 years in biostable polymeric structures to minimize the associated
regards to design and testing of bioresorbable vascular grafts, potential failure modes. In general, three classic models of
and this historic work suggests that this approach to vascular vascular tissue engineering have been prominent. The earligrafts is promising. As best stated by Howard Greisler and est, originated by Weinberg and Bell in 1986, and most
associates, The resorbable graft represents a paradigm shift in widely studied model, is the use of collagen gels with encapvascular prostheses. Unlike conventional prostheses whose sulated cells [13]. Generally, in this model the outer layer of
effectiveness depends by and large upon
How much in vitro fabrication, the graft wall is composed of a collaan inherent resistance to a normal healgen gel containing fibroblasts to form
ing process, resorbable grafts work with if any, is really required beyond an adventitia, which surrounds a
scaffolding?
the body, providing a framework for
collagen gel layer containing smooth
well-established healing patterns. It seems intuitive that by muscle cells to form the media, and a monolayer of ECs
augmenting rather than opposing these patterns, a more effi- forms a neointima. In the majority of studies on this model,
cacious vascular graft should result [9]. One potential down- one major limitation is the requirement of a synthetic graftside to this innovative work was the use of large diameter like external support structure, often a Dacron graft, to
fibers (140 or 250 m) of various biodegradable polymers allow the overall structure to withstand arterial forces (i.e.,
knitted to form the graft walls the large diameters are prob- pressure). One concern with the clinical application of such
ably not optimum for promoting arterial regeneration, but a model would be the continuous potential for delamination
were adequate in animal models. Whether or not regeneration of part or the entire biologic structural portion, as well as
capacity would translate to human success is unknown, and the threat of nontreatable infections due to the synthetic
graft wrapping. The second model utilizes a bioresorbable
probably questionable.
Bionanotechnology, or more specifically electrospinning, is tissue-engineering scaffold for seeding of the arterial cellular
a polymer processing technique that may be able to address components, and allows those cells to proliferate and fabrithe limitations of the knitted, large diameter fibers used in cate a native ECM in place of the resorbable structure over
creating bioresorbable vascular grafts. Electrospinning is time in culture (often in a dynamic bioreactor for construct
currently being utilized in the authors laboratory to revital- conditioning/strengthening) [14,15]. The major disadvantage
ize the concept of a bioresorbable vascular graft, and could of this model is the weeks to months (labor and cost intenpotentially prove to be a vital technology in the development sive) required to develop any substantial mechanical integof a tissue-engineered blood vessel [1012]. Electrospinning rity. The final model could be referred to as sheet engineerallows for the generation of nonwoven fibrous structures that ing, or self-assembly model where the individual cellular
mimic the nanoscale fiber dimensions of the native ECM, components (fibroblasts and smooth muscle cells) are grown
the fiber composition (i.e., collagens and elastin) of the ECM in culture under conditions that allow excessive ECM
and the mechanical properties of the native structures. This production [16]. This model of assembly requires several
establishes a well-defined architecture capable of guiding cell weeks to produce a continuous sheet-like structure which
migration, proliferation, differentiation and function (tissue can be lifted from the culture dish and wrapped around a
development and maintenance). Thus, the authors group is mandrel to form tubular layers of a vascular graft. These
conducting extensive research regarding the application of constructs, unlike collagen gel structures, do exhibit subelectrospun, nonwoven seamless tubular structures composed stantial mechanical integrity and can withstand arterial
of natural and synthetic biodegradable polymers as acellular forces. One major down side with sheet engineering and the
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Affiliations
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