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Olympus Microscope Manual - Draft
Olympus Microscope Manual - Draft
General rules:
If you want to use the microscope for more than 2 hours: make a
reservation (list available on bioteclab dropbox)
Contact:
Glenn.vandestaey@cit.kuleuven.be
Kim.vekemans@cit.kuleuven.be
Rob.vandenbroeck@cit.kuleuven.be
1. Sample preparation
Apply three droplets of your sample on a sample glass, as shown in figure 1. Drop
a cover glass on each droplet, while taking care that you only use one cover
glass! Attach the cover glass to the sample glass using nail polish at the borders
(see figure 2).
Wait for the nail polish to dry. When dried, insert the sample on the sample stage
of the microscope with the cover glasses downwards (which is why the cover
glasses are attached with nail polish).
2. Starting up procedure
Choose the user IX83-user and insert the password obtained during the
training.
3. Closing procedure
Remove all stored images from the S-drive. The solid state drive is only used
for quick measuring, not for storage!
To protect the objects against dust, it is important to cover the objective hole
in the stage!
4. Interface Overview
Error: Reference source not found shows the start-up interface of cellSens
Dimension. The major functions, illustrated with text boxes, will be discussed in
detail in the following subsections.
4.1.
An overview of the top part of the Camera Control Panel is shown in Figure 1.
Click on the icon for Acquisition Settings, displayed in the top left corner as
part of the Camera Control Panel (see Figure 1).
For Snapshot:
Choose a file type (e.g. vsi or tiff). The vsi extension saves
all information, but can only be opened and manipulated
in the cellSens Dimension program. Tiff on the other hand
is the best option for further analysis using Matlab.
b. Expand Camera and go to General. See that both boxes for horizontal
and vertical mirroring are checked, as shown in Figure 1.
Live/Snap/Movie recording
a. Click on Live if you want to see what the microscope is seeing that
exact moment and place (this will appear in the middle of the screen),
b. click on Snap if you want to take a snap shot of that specific moment
at that specific place,
c. and check the box on Movie recording if you want to make a video.
The program can adjust the exposure time automatically or you can choose
one manually, as can be seen on Error: Reference source not found. Note that
'eyepieces,camera,50/50' are adjusted under Microscope Control.
The Auto-Focus function can literally be used to automatically bring the image
in focus. If not all objects are in the same plane however, this function
randomly chooses one object to focus upon (which causes the other objects to
be out of focus).
It is possible to take pictures in grey scale and in color, using the Toggle
RGB/Greyscale mode'-button.
4.2.
Microscope Control
You can choose from 5 objectives: 4x, 10x, 20x, 40x, or 100x. The 100x
objective is an oil immersed lens, therefore you need to use oil immersed
samples and clean the microscope with a tissue after use.
4.3.
Stage Navigator
4.4.
Process Manager