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Raditus Used Because It Can Conduct Respiration Although The Body Is Not
Raditus Used Because It Can Conduct Respiration Although The Body Is Not
Net volume
change in 30
minutes with no
CO2 absorbed
Amount of
carbon
dioxide
absorbed
Vol 1
Vol 2
Vol 1 + Vol 2
0.4
0.2
0.6
Respiratory
quotient=
(carbon dioxide
produced/oxygen
absorbed)
(Vol 1 + Vol 2) /
Vol 1
1.5
B. Discussion
In this observation, we concern to measure the respiratory quotient of
a plant, in this case used the the sprout of Phaseolus radiatus. Phaseolus
raditus used because it can conduct respiration although the body is not
develope well, yet. In order to measure the respiratory quotient, its used a
device called respirometer that can measure the gases that organism takes in
and express. The respirometer consist of two tube that connected with capilary
U-tube that contain eosin. Eosin used as an indicator of the influx of oxygen
from the outside. One tube contain of potassium hydroxide solution and plastic
containing seeds above it. In the upper part of the first tube placed a syringe
that used to balance the eosin in U-tube. The second tube contain potassium
hydroxide solution plus water to equal the volume of the seeds in the other
tube. Potassium hydroxide used to bind the oxygen that respirate by the seed so
it can be measured. Both of tube filled with filter paper rolled as a medium to
bind O2, so that in the struggle for oxygen tube respirometer occurred between
15% KOH solution with the Phaseolus radiatus.
In the first experiment from balancing process we need 0,5 scale of O 2 in
first balancing and 0,1 scale O2 in second balancing, so totally we get 0,4 scale
oxygen that was inserted in the tube. Oxygen consumption cannot be measured
simply by putting sprout in the test tubes because beans are also producing
CO2. Any change in gas volume will be due to both O2 consumption and CO2
CHAPTER V
CONCLUSION AND SUGGESTION
A. Conclusion
Based on the observations that have been done, it can be concluded that:
2.
3.
QUESTION
UNIT 15
1. what respiratory substrate would you expect to find in seeds?
Answer: Seeds such as sunflower contain oils. Other seeds contain more
carbohydrates. All contain proteins raw materials for the initial growth of
seedlings.