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Contributions To The Study of The Biochemical Variability of The Polyphenols Existing in 8 Thymus Pulegioides Populations From Northern Moldavia
Contributions To The Study of The Biochemical Variability of The Polyphenols Existing in 8 Thymus Pulegioides Populations From Northern Moldavia
1. Introduction
If at the beginning of the last century
over 450 species were registered as
belonging to the Thymus genus, nowadays
it is accepted that this genus contains at
1
2
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Bulletin of the Transilvania University of Braov. Series VI Vol. 4 (53) No. 2 - 2011
Location
Tp1
Tp2
Tp3
Tp4
Tp5
Tp6
Tp7
Tp8
Collection date
(2010)
24.05
06.06
06.06
06.06
06.06
06.06
06.06
06.06
Table 1
Altitude (m),
aprox.
75
650
600
600
600
600
880
600
17
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Bulletin of the Transilvania University of Braov. Series VI Vol. 4 (53) No. 2 - 2011
19
Table 2
Location
Tp1
Albesti
0.29530.0008
2.04590.0109
Tp2
Tp3
Tp4
Tp5
Tp6
Brosteni
Farcasa 1
Farcasa 2
Farcasa 3
Potoci
0.82450.0011
0.14250.0009
0.37430.0010
0.29170.0014
0.29920.0004
4.07150.0087
1.72010.0064
2.21810.0188
2.24860.0203
2.41980.0119
Tp7
Tp8
Valea Putnei
Vama
0.36160.0008
0.32560.0007
3.34370.0107
2.46600.0177
Table 3
The semiquantitative determination by HPLC of flavonoids and phenolcarboxylic acids in
concentrated extracts (DER=0,125:1 g/mL) of Thymus pulegioides 2010 samples
Sample
Location
Chlorogenic
acid
Tp1
Albesti
0.88
Tp2
Brosteni
0.15
Tp3
Farcasa 1
0.16
Tp4
Farcasa 2
0.09
Tp5
Farcasa 3
1.35
Tp6
Potoci
0.08
Tp7
Valea Putnei
0.09
Tp8
Vama
0.45
* <l.d. : value under the detection limit
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Bulletin of the Transilvania University of Braov. Series VI Vol. 4 (53) No. 2 - 2011
21
30.393
re
a:
46
15
.8
8
mAU
120
100
80
60
40
0
10
20
30
53.332
A11.931
re
a:
4.
02
99
A34.004
re
a:
1.1
44
83
20
40
50
60
min
mg % g
140.00
120.00
100.00
80.00
60.00
40.00
20.00
0.00
Tp1
Tp2
Tp3
Tp4
Tp5
Tp6
Tp7
Tp8
Fig. 5. The variability in rosmarinic acid content determined by HPLC for Thymus
pulegioides samples.
Samples: Tp1=Albesti; Tp2=Brosteni; Tp3=Farcasa-1; Tp4=Farcasa-2;
Tp5=Farcasa-3; Tp6=Potoci; Tp7=Valea Putnei; Tp8=Vama.
4. Conclusion
Analyzing the data presented we
conclude that there is a fairly large
chemical
variability regarding
the
quantitative aspect of the various
polyphenolic components even if they are
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Bulletin of the Transilvania University of Braov. Series VI Vol. 4 (53) No. 2 - 2011
5. Aknowledgement
The paper was presented at the: al IV lea
Simpozion National de Etnofarmacologie
cu participare internationala cu tema
"Etnofarmacologia la interfata bioalimentfitomedicament" - iunie 2011 Brasov
Sirnea.
References
1. Blaschek, W., Ebel, S., Hackenthal, E.,
Holzgrabe, U., Keller, K., Reichling,
J., Schulz, V.: Hagers Handbuch der
Drogen und Arzneistoffe, 7 ed.,
Springer, Berlin, CD-ROM, 2006.
a
thin
layer
chromatography atlas, 2.ed. Berlin.
Springer, 1996.
4. *** Farmacopeea Romana, 10 ed., Ed.
Medicala, Bucuresti, 1993.