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Early History of Biological Warfare
Early History of Biological Warfare
Another BIOS project focuses on engineering new molecular pathways that result in pigment changes in bacteria upon exposure to a variety of bacterial and viral
pathogens. A separate project seeks to engineer biological circuits in the E. coli bacterium for sensing biological
agents based on the well-known lac and mal operons
as models.
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ELECTRONIC:
SEE ALSO
Biodetectors
Bio-Engineered Tissue Constructs
Biological and Biomimetic Systems
Biological Warfare
Biological Warfare, Advanced Diagnostics
Bio-Optic Synthetic Systems (BOSS)
Biosensor Technologies
Biological Warfare
JUDYTH SASSOON
Early History of
Biological Warfare
Examples of the use of biological weapons exist in ancient
records. In the sixth century B.C., Assyrians poisoned enemy wells with ergot, a toxin derived from mold that
grows on rye. Other records of battles document the use
of diseased corpses to poison wells. In 1346, plagueinfected corpses and carcasses were catapulted into Kaffa,
a city in current day Crimea, by the Tartar army. The
epidemic that resulted may have eventually led to the
great Black Plague that aficted Europe. In 1710, the Russian army used a similar military strategy when it invaded
Sweden. The Spanish are reported to have contaminated
French wine with blood taken from people suffering from
Modern History of
Biological Warfare
During the twentieth century, modern scientic methods
led to the development, renement, and stockpiling of
weapons of biological warfare by governments throughout the world. During World War I, Germany developed a
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Biological Warfare
biological warfare program based on the bacterium Bacillus anthracis and a strain of Pseudomonas known as
Burkholderia mallei, which causes glanders disease in
cattle. Dr. Anton Dilger, a German agent living in Washington D.C., reportedly grew anthrax and glanders bacteria in
his home and then inoculated thousands of horses and
cattle that were shipped to Allied troops in Europe. Many
of the animals perished and hundreds of the troops exposed to these animals were secondarily infected by the
diseases.
During World War II, prisoners in German Nazi concentration camps were infected with pathogens, such as
Hepatitis A, Plasmodia spp., and two types of Rickettsia
bacteria, during studies allegedly designed to develop
vaccines and antibacterial drugs. A large reservoir in
Bohemia was poisoned with sewage by the German
army in 1945.
Between 1918 and 1945, the Japanese government
conducted extensive biological weapon research at Unit
731 in occupied Manchuria, China. Prisoners of war were
infected with a variety of pathogens, including Neisseria
meningitis (meningitis), Bacillus anthracis (anthrax),
Shigella spp. (shigellosis), and Yersinia pestis (black
plague). Estimates are that over 3,000 prisoners died as a
result of infection by these biological pathogens or execution following such infections. In 1941, the Japanese released an estimated 150 million potentially plague-infected eas from aircraft over cities in China and Manchuria.
After these infectious agents were released, outbreaks of
plague occurred in many Chinese villages. In addition,
approximately 10,000 illnesses and 1,700 deaths occurred
among Japanese troops.
Driven by reports of Japanese and German programs
to develop biological weapons, the Allies embarked on
vigorous efforts to develop their own biological weapons
during World War II. Britain produced ve million anthrax
cakes at the UK Chemical and Biological Defense Establishment at Porton Down with the intent of dropping them
on Germany to infect the food chain. These weapons were
never used. British open-air testing of anthrax weapons in
1941 on Gruinard Island in Scotland rendered the island
inhabitable for ve decades.
The United States governments biological warfare
facility was headquartered at Fort Detrick in Maryland
beginning in 1942. Weapons were also tested and produced in Colorado, Arkansas and Utah. Many different
agents were studied including the bacteria that cause
anthrax, plague, botulism, Q fever, and staphylococcal
infections. Several viruses were also included in the research. The U.S. Army conducted a study in 19511952
called Operation Sea Spray to study wind currents that
might carry biological weapons. As part of the project
design, balloons were lled with Serratia marcescens
(then thought to be harmless, but easily identiable) and
exploded over San Francisco. Shortly thereafter, there
was a corresponding dramatic increase in reported pneumonia and urinary tract infections in the region.
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ELECTRONIC:
Rhode Island Department of Health: Bioterrorism Preparedness Program History of Biological Warfare and
Current Threat <http://www.healthri.org/environment/
biot/history.htm> (March 12, 2003).
SEE ALSO
Anthrax Weaponization
Biological and Toxin Weapons Convention
Bioterrorism
Chemical Warfare
Infectious Disease, Threats to Security
Viral Biology
Weapons of Mass Destruction
blood cells provides a very quick indication of the presence of a biological threat. The second method involves
the development of a wearable, non-invasive diagnostic
device that detects a broad-spectrum of biological and
chemical agents.
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ELECTRONIC:
Biological Warfare,
Advanced Diagnostics
The Advanced Diagnostics Program is funded by the
Defense Advanced Research Projects Agency of the United
States government (DARPA). Its objective is to develop
tools and medicines to detect and treat biological and
chemical weapons in the eld at concentrations low enough
to prevent illness. Challenges to this task include minimizing the labor, equipment, and time for identifying biological and chemical agents.
One area of interest includes development of eld
tools that can identify many different agents. To accomplish this goal, several groups funded under the advanced
diagnostics program have developed eld-based biosensors that can detect a variety of analytes including fragments of DNA, various hormones and proteins, bacteria,
salts, and antibodies. These biosensors are portable, run
on external power sources, and require very little time to
complete analyses.
A second focus of the advanced diagnostics project is
the identication of known and unknown or bioengineered
pathogens and development of early responses to infections. Many viruses act by destroying the ability of cells
to replicate properly. One group funded under the advanced diagnostics program is studying the enzyme 5monophosphate dehydrogenase (IMPDH), which produces
products that are required for synthesizing nucleic acids,
such as RNA and DNA, both of which are essential for
proper cell replication. This group seeks to develop novel
drugs based on IMPDH, which can cross into cells and
thwart viral infection.
A nal goal is to develop the ability to continuously
monitor the body for evidence of infection. Researchers
are addressing this goal in two ways. The rst involves
engineering monitoring mechanisms that are internal to
the body. In particular, groups funded under the initiative
are developing bioengineered white blood cells to detect
infection from within the body. Often genetic responses to
infection occur within minutes of infection so analysis of
SEE ALSO
Biodetectors
Biological Warfare
Biomedical Technologies
Biosensor Technologies
Bioterrorism
Bioterrorism, Protective Measures
Biological Weapons,
Genetic Identication
Biological weapons are weapons whose payload consists of microorganisms that can cause infections, or the
toxic components of the microorganisms. Examples of
microorganisms include viruses (e.g., smallpox, Ebola,
inuenza), bacteria (e.g., Bacillus anthracis, Clostridium
botulinum, Yersinia pestis) and protozoa. The most prominent example of a toxic component is the variety of toxins
produced and released from bacteria (e.g. neurotoxins
produced by Clostridium).
Genetic technologies can be useful in the detection of
biological weapons. Of particular note is the polymerase
chain reaction, or PCR, which uses select enzymes to make
copies of genetic material. Within a working day, a target
sequence of genetic material can be amplied to numbers
that are detectable by laboratory tests such as gel electrophoresis. If the target sequence of nucleotides is unique to
the microorganism (e.g., a gene encoding a toxin), then
PCR can be used to detect a specic microorganism from
among the other organisms present in the sample.
Hand-held PCR detectors that have been used by
United Nations inspectors in Iraq during their weapons
inspections efforts of 20022003 purportedly can detect a
single living Bacillus anthracis bacterium (the agent of
anthrax) in an average kitchen-sized room.
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