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Lipid Metabolism Card PDF
Lipid Metabolism Card PDF
Lipid Metabolism Card PDF
LDL
LDLR
CHOL
LIPC
BA
SR-B1
IDL
ApoER
LPL
Liver
ABCG5/8
ABC11
ABCB4
ABCC2
VLDL
Chylo
Remn
LPL
HDL
ApoE
ApoC2
CETP
Chylomicron
Cells and organisms use lipoproteins to move hydrophobic lipid molecules, which are not water
soluble, through the aqueous blood and tissue lymph environment. Lipoprotein particles are defined
by their complement of associated apolipoproteins (Apo) and their content of cholesterol (CHOL),
triglyceride (TG) and phospholipid that each particle carries. This protein and lipid content defines the
particles buoyant density and subdivides them into 4 major classes: high density lipoprotein (HDL),
low density lipoprotein (LDL), very low density lipoprotein (VLDL), and chylomicrons.
HDL is formed by the transfer of cholesterol and phospholipids onto apolipoproteinA-1 (ApoA-1) to
generate pre -HDL. This process is catalyzed by the ATP-binding cassette (ABC) A1 (ABCA1)
transporter, which is expressed in the peripheral tissues, intestine and liver (not shown here). The
cholesterol in the nascent pre -HDL is then esterified by lysolecithin cholesterol acyltransferase
(LCAT) as part of a process that generates mature spherical HDL. ABCG1, another ABC transporter,
is able to load more cholesterol onto mature HDL from peripheral tissues and along with ABCA1 is
important in allowing macrophages to efflux artery wall cholesterol, which prevents atherosclerotic
vessel disease. HDL cholesterol-esters are taken up by scavenger receptor BI (SRBI) in liver and after
hydrolysis the resulting free cholesterol is metabolized to bile acids (BA). The bile acids along with
more free cholesterol is excreted into the digestive tract via biliary secretion in a process that again
utilizes ABC transporters (ABCG5/ABCG8, ABCB11, ABCB4 and ABCC2).
Conversely, in the small intestine absorbed dietary fatty acids are converted into triglycerides (TG) and
are packaged and secreted into the bloodstream as chylomicrons, a lipoprotein particle rich in TG and
apoB-48. TG in this lipoprotein is rapidly hydrolyzed into free fatty acids by lipoprotein lipase (LPL),
leading to the formation of chylomicron remnants (Chylo Remn), which are taken up by the liver via
the apoE receptor (ApoER). Dietary cholesterol is also packaged into HDL particles by the action of
ABCA1 and ABCG1, and as HDL circulates there is an increase in its apoC2 and apoE ratio because
of a protein exchange between HDL and VLDL. Cholesterol ester from HDL is also transferred to
VLDL remnant particles (IDL) by the action of cholesteryl ester transfer protein
(CETP). IDL loses most of apolipoprotein except apoB and is converted
to LDL by the action of hepatic lipase (LIPC).
LCAT
ABCG1
ApoB
TG
ABCG1
pre-HDL
CHOL
NPC1
ABCG5
CHOL
ABCA1
ABCA1
ABCG8
Intestine
Peripheral
Tissues
ApoA1
www.abcam.com
Produced in collaboration with Michael Fitzgerald PhD
Clonality
Applications
Host
ABCA1
ABCA1
ABCA1
ABCA1
ABCB4
ABCG1
ABCG1
ABCG5
ABCG8
AMPK gamma 1
Apolipoprotein A I
Apolipoprotein A I
Apolipoprotein B
Apolipoprotein B
Apolipoprotein B
Apolipoprotein B
Apolipoprotein CII
Apolipoprotein E
Apolipoprotein E
BMAL1
BMAL1
Caveolin 1
M
P
P
M
M
M
P
P
P
M
P
M
P
M
P
P
P
M
P
M
P
P
Mouse
Rabbit
Rabbit
Rat
Mouse
Rabbit
Rabbit
Rabbit
Rabbit
Rabbit
Goat
Rabbit
Goat
Mouse
Rabbit
Sheep
Goat
Rabbit
Rabbit
Mouse
Rabbit
Rabbit
CETP
CETP
Cholesterol Oxidase
COX2 / Cyclooxygenase 2
M
P
P
P
ELISA, IP, WB
WB
Conjugation, Dot, ELISA, IP, WB
ICC, IHC-P, WB
Mouse
Rabbit
Goat
Goat
Species
Reactivity
Datasheet
www.abcam.com/ab
18180
14146
7360
48263
24108
52617
36969
45279
45280
32382
7613
52945
7616
20624
20737
8947
7618
52607
52924
53215
49421
Clonality
Applications
M
P
P
M
P
M
P
M
P
M
P
M
P
IHC-Fr, IHC-P
ELISA, WB
ELISA, WB
ELISA, IHC-Fr
EIA, IP, RIA, WB
Flow Cyt, IP, WB, ICC/IF, IHC-P
ELISA, WB
WB
ELISA, IHC-P
Flow Cyt, ICC/IF, IHC-P, IP, WB
Flow Cyt, ICC, IHC-Fr, WB
WB
WB
18199
2726
19012
34495
M
M
P
M
M
P
P
M
P
M
23672
*= predicted to react
Host
Species
Reactivity
Rabbit
Rabbit
Chicken
Mouse
Rabbit
Rabbit
Chicken
Mouse
Rabbit
Rabbit
Rabbit
Mouse
Rabbit
Hu, Ms,
Hu, Ms,
Hu
Hu
Hu
Hu
Hu
Hu
Hu
Hu, Ms,
Hu, Ms,
Hu
Hu, Ms,
RMk*
Mouse
Mouse
Mouse
Rabbit
Mouse
Rabbit
Rabbit
Chicken
Mouse
Rabbit
Mouse
Datasheet
www.abcam.com/ab
Rat
Rat
Rat
Rat
21704
52237
15863
34788
51419
51060
15870
57471
14519
52818
30532
56493
Bb*, Chmp*,
Hu, Ms, Rb
Hu
Hu, Rat
Hu, Ms*, Rat*
Rat
Hu, Ms, Rat
Ms, Rat
Hu
Hu
Hu
Hu
65364
28320
23346
3373
53193
37231
52629
24603
16419
63977
35056
62543
Apolipoprotein E (ab52607)
Host
Mouse
Host
Rabbit
ABCG1 (ab52617)
Clonality Applications
M
ICC, IHC-P, WB
Product
COX2 / Cyclooxygenase 2
COX2 / Cyclooxygenase 2
HDL
HDL
HDL
LCAT
LDL
LDL
LDL (Copper oxidized)
LDL Receptor
LDL Receptor
LIPG
LIPG
ABCA1 (ab18180)
Clonality Applications
M
ELISA, ICC/IF, IHC-P, IP, WB
www.abcam.com/cardiovascular
Clonality Applications
M
ICC/IF, IHC-P, IP, WB
Host
Rabbit
Clonality Applications
Host
M
Flow Cyt, ICC/IF, IHC-P, IP, WB Rabbit