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Bio 366 Sdsu Exam 2
Bio 366 Sdsu Exam 2
Bio 366 Sdsu Exam 2
Name______________________
6. How does the telomerase enzyme differ from other DNA polymerases?
a. does not require a primer
b. does not require a template
c. copies only A-rich sequences
d. requires an associated RNA containing the template sequence
e. c and d
7. Which of the following proteins binds lactose in order to activate E. coli lac operon
transcription?
a. RNA polymerase
b. sigma factor
c. catabolite activator
d. lac repressor
e. -galactosidase
8. Why is DNA looping required for activation of the E. coli glutamine synthetase gene
(gln) by the nitrogen regulatory protein C (NtrC)?
a. the polymerase binds to two sites far apart from each other
b. 54 binds far upstream of the start site
c. NtrC protein binds to an upstream enhancer sequence and to the polymerase
d. gln gene promoter has no control regions
e. both 54 and 70 must bind to the promoter
9. Which of the following is characteristic of eukaryotic genes whose promoters
possess CpG islands instead of TATA boxes?
a. only found in lower eukaryotes
b. multiple transcription start sites in a region of 20-200 bp
c. transcribed by a modified pol II
d. promoter doesnt bind transcription factors
e. transcribed infrequently
10. Which of the following is true for analysis of transcription control elements using
the linker scanning method but not the deletion analysis method?
a. the overall size of the fragment linked to a reporter gene is kept constant
b. plasmid transfection is used to measure the effects of change in sequence
c. a different reporter gene is required
d. the results can only map elements to fragments larger than 1 kbp
e. cells used for transfection must contain transcription factors that bind to the elements
Name______________________
11. The eukaryotic Pax6 gene contains many transcription control elements. Which of
the following correctly describes their location in the gene?
a. within exons only
b. upstream of the start site only
c. all downstream of the promoters
d. within introns only
e. within introns and upstream of the start site
12. Which of the following incorrectly describes the properties of eukaryotic
transcription factors?
a. always bind to a specific DNA sequence
b. DNA-binding domain is separate from activation or repressor domain
c. DNA-binding domain is always at the N-terminal end of the protein
d. activation domain can be at the N- or C-terminal end of the protein
e. activation domain of one factor can work with DNA-binding domain of another
13. Which of the following is characteristic of the DNA-binding domain in bZIP and
bHLH transcription factors?
a. helix-turn-helix motif
b. coiled-coil amphipathic helices
c. ability to bind zinc ions
d. rich in acidic amino acids
e. unstructured region
14. Which of the following distinguishes general transcription factors from other types
of transcription factors?
a. not tissue-specific
b. required for all genes transcribed by pol II
c. all contain a TATA box-binding subunit
d. contain acidic activation domains
e. a and b
15. Which of the following incorrectly describes the properties of the mediator complex
involved in eukaryotic gene transcription?
a. binds to pol I, pol II, and pol III
b. contains more than 20 subunits
c. some subunits are required for only a subset of pol II genes
d. forms a bridge between transcription factors and the pol II pre-initiation complex
e. required for transcription in both lower and higher eukaryotes
16. Which of the following correctly identifies the location of the promoter region for
RNA polymerase III genes?
a. downstream of start site
b. ~50 bp upstream of start site
c. ~90 bp upstream of start site
d. ~50 bp region overlapping start site and one at ~155 bp upstream
e. none of the above
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Name______________________
17. Which of the following enzymatic reactions occurs first when mRNA precursors are
capped?
a. addition of GTP to 5 end of mRNA
b. removal of gamma phosphate from mRNA 5 end
c. removal of pyrophosphate from mRNA 5 end
d. methylation of GTP at the 7 position
e. methylation of ribose on first nucleotide in the mRNA chain
18. Which of the following correctly describes the location of the A residue branch point
utilized in splicing of eukaryotic introns?
a. at the very 3 end of the intron
b. near the 5 end of the intron
c. in the exon near the 5 splice site
d. 20-50 bases upstream of the 3 end of the intron
e. in the exon downstream of the 3 splice site
19. Which of the following serves as a binding site for SR proteins (rich in ser and arg)
that facilitate recognition of correct splice sites in mRNA precursors?
a. pyrimidine-rich region
b. cap
c. poly(A) tail
d. U4 snRNA
e. exonic splicing enhancer
20. Which of the following protein factors recognizes the AAUAAA polyadenylation
signal sequence on precursor mRNAs??
a. CPSF
b. PABPI
c. maturase
d. CStF
e. TAP
21. Hair cells in the inner ear system of vertebrates respond to particular sound
frequencies depending on their position. Which of the following plays a major role in
this phenomenon?
a. different isoforms of the fibronectin protein
b. multiple K+ ion channel genes
c. multiple Ca+ ion channel genes
d. alternatively spliced mRNAs encoding a Ca+ ion channel
e. alternatively spliced mRNAs encoding a K+ ion channel
22. Which of the following generally determines whether a mRNA will be degraded
rather than display translational inhibition when bound to miRNAs or siRNAs?
a. processing pathway of the miRNA or siRNA precursor
b. perfect base-pairing to mRNA target
c. base-pairing to coding region of mRNA
d. base-pairing to 5 UTR
e. short poly(A) tail
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Name______________________
Name______________________