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THESIS
By :
MUHAMMAD HIRZI NUGRAHA
11/311664/KG/08807
N
4
Mean
564,75
Standard Deviation
85,496
0,05%
0,1%
0,15%
4
4
4
350,50
245,75
141,75
47,592
22,036
11,236
Table 2. The result of LSD0,05 mean difference of fungal count numbers in thermoplastic
nylon between treatment groups.
Group
BW0,05
BW0,1
BW0,15
BW0,05
BW0,1
BW0,15
BW0
104,750*
208,750*
214,250*
104,000*
319,000*
423,000*
BW0
* significant difference
Results of LSD0,05 test (Table 2) indicate that there were significant differences of
fungal count mean in thermoplastic nylon between all treatment groups with variation of
billimbi fruit ethanolic extract concentration.
Discussion
The result showed decreasing fungal count tendency after exposure of bilimbi fruit
ethanolic extract. One way ANOVA test showed the effect of bilimbi fruit ethanolic extract
concentration variation as a denture cleaner on C. albicans fungal count.
The reduction of fungal count after exposured with bilimbi fruit ethanolic extract for 5
minutes showed that bilimbi fruit ethanolic extract has an antifungal property. This result
were not different with the result of Karon et al15 stated that bilimbi fruit has an antifungal
property. Bilimbi fruit has secondary metabolite compound that is phenolic (antifungal)
consist of flavonoid and tannin11.
The mechanism of flavonoid as an antifungal is by denaturating microbes cell wall
12
protein . The outermost layer of C. albicans cell wall contains negative charged phosphate
cluster. This enable C. albicans to have the capability for binding positive charged ion16.
Flavonoid is fenol compound derivate that have OH- cluster and could donor H+ ion when
reacting water, making phenoxide and oxonium ions17. C. albicans cell wall could be
denaturated because of phenolic compound that has acidic nature bind with the outermost
layer of C. albicans cell wall. H+ ion diffused into cytoplasm because denaturated cell wall
decrease cytoplasmic pH. The reduction of cytoplasmic pH could harms microorganisms in
ways of inhibiting enzyme activities and membrane transport proteins 18.
Tannin could disturb cell wall integrity from microbe in ways of inhibiting chitin
synthesis13. Chitin synthesis need the activity of carbohydrate enzyme as
glucosyltransferase19. Chitin work to keep C. albicans cell wall integrity20.
Glucosyltransferase will be activated on meeting the substrate and cofactor 21. Cofactor from
glucosyltransferase is a divalent metal like Mn2+ 22. Tannin could bind metal ion so that the
activation of glucosyltransferase is inhibited23. The broken cell wall compromise the adhesion
of C. albicans24.
The result of LSD0,05 test (Table 2) showed that there are significant differences of
fungal count among all treatment groups (p<0,05). It happened because of the difference of
phenolic substance inside various bilimbi fruit ethanolic extract concentration 25. The increase
of antifungal concentration (phenolic) inside bilimbi fruit will give more significant effect
against fungal26. According to Pelzar and Chan14, the higher antifungal consentration will
affect the number of killed fungal.
Conclusion
According to the result of this study, it could be concluded that: bilimbi fruit ethanolic
extract with 0,05%, 0,1%, and 0,15% as nylon thermoplastic denture cleanser have
significant effect against the growth of Candida albicans.
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