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Planta Medica 2003. 69, 1068-1070 (Mangiferin)
Planta Medica 2003. 69, 1068-1070 (Mangiferin)
Abstract
Letter
1068
Affiliation: 1 Department of Pharmaceutical Sciences, National Research Centre, Dokki, Cairo, Egypt 2 Department of Pharmaceutical Sciences, University
of Antwerp, Antwerp, Belgium
Correspondence: Dr. A. A. Shahat Department of Pharmaceutical Sciences
University of Antwerp Universiteitsplein 1 2610 Antwerp Belgium Fax:
+32 3 820 27 09 E-mail: shahat@uia.ua.ac.be
Received: April 8, 2003 Accepted: July 26, 2003
Bibliography: Planta Med 2003; 69: 10681070 Georg Thieme Verlag
Stuttgart New York ISSN 0032-0943
Letter
Plant material: Leaves of Bombax malabaricum DC. were collected at Giza Street, Area of Imbaba, Giza, Egypt in May, 2000. The
plant was kindly identified by Prof. Dr. K. H. Al-Batanony, Professor of Taxonomy, Faculty of Science, Cairo University to whom
the authors are greatly indebted. A voucher specimen (Bombaceae/Bombax malabaricum/052000) is kept at the Herbarium
of the NRC, Dokki, Cairo, Egypt.
A reference sample of mangiferin was obtained from Extrasynthse, France. A sample of shamimin was obtained from Prof. S.
Faizi, University of Karachi, Pakistan [6].
Extraction and isolation: 500 g of the dried powdered leaves were
defatted with petroleum ether (40 60 8C). After drying, the residue was extracted with 70 % EtOH (4 1000 mL). The alcohol extract was evaporated under reduced pressure and kept in the refrigerator overnight. After filtration the supernatant was successively extracted with CHCl3, EtOAc and n-BuOH (5 300 mL
each). The n-BuOH fraction (4 g) was subjected to column chromatography on polyamide (60 3 cm) and eluted first with H2O
(150 mL), and then with a gradient of H2O and MeOH with an increasing amount of MeOH, in steps of 10 % (elution volume each
time 200 mL). Fractions eluted with 60 % MeOH were collected
and concentrated. Repeated column chromatography on Sephadex LH-20 (Pharmacia) (60 1.5 cm) using MeOH as eluent
(3 200 mL) gave compound 1 (98 mg).
Mangiferin (2-b-D-glucosyl-1,3,6,7-tetrahydroxyxanthone) (1). UV
(MeOH): lmax (log e) = 243 (4.56), 259 (4.61), 318 (4.26), 368 nm
1069
Table 1
13
Letter
Carbon No.
13
1
2
3
4
4a
5
6
7
8
8a
9
9a
10a
1
2
3
4
5
6
161.76
107.56
163.81
93.31
156.21
102.56
154.17
143.76
108.00
111.63
179.07
101.29
150.82
73.09
70.62
79.14
70.26
81.53
62.78
C-NMR (d)
6.35, s
6.84, s
7.36, s
4.58, d, J = 9.8Hz
4.03, dd, J = 9.1, 9.0 Hz
3.20, m
3.14, m
3.16, m
3.68, br d, J = 11.6 Hz
3.40, dd, J = 11.6, 5.5 Hz
(4.22) [8]. ESI-MS (positive ion mode): m/z = 423 ([M + H]+). 1HNMR (400 MHz, DMSO-d6): Table 1. 13C-NMR (100 MHz, DMSOd6): Table 1.
Acknowledgments
Dr. M. Claeys (Department of Pharmaceutical Sciences, University of Antwerp) is kindly acknowledged for recording mass spec-
1070
References
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