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BevTrac

Mobile Quality Systems LLC

Practical Winery Sanitation


Napa Valley Wine Technical Group
March 26, 2009
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Agenda
WineMicrobiologyOverview
WhatisSanitation?
EffectsofPoorSanitation
SelectingCleanersfortheWinemakingProcess
SelectingSanitizersfortheWinemakingProcess
RecommendedSanitationMethodsforEquipmentandInfrastructure
CuttingEdgeCleaning/SanitationPracticesforWineries
MethodstoMonitorEffectivenessofCleaningandSanitationPractices
EstablishingMicrobiologicalCriteriaforWinerySanitationPractices
RequirementsforaSuccessfulandSustainableWinerySanitationProgram
BenefitsofaSuccessfulandSustainableWinerySanitationProgram

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Wine Microbiology Overview

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WineMicrobiology
WheredoWineSpoilageMicroorganismsComeFrom?
Incominggrapes
Insects(e.g.fruitflies)
Contaminatedwineryequipmentandcooperage

Crushequipment
Barrels
Tanks
Winehosesandtransferlines
Generalwineryequipment
Bottlingline

Obtainingcontaminatedwinefromotherwineries
Environmentalcontamination atmosphere,water

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WineMicrobiology
MajorFactorsAffectingGrowthofWineMicrobes

Sanitationpractices
pH
Oxygen
Nutrients
Winestoragetemperature
Moistureavailability
Lagphasetime
Generationtime

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WineMicrobiology
HowdoWineMicroorganismsSpoilWine?
Yeast
Saccharomycescerevisiae
Secondaryfermentationinbottleto
producehaze&CO2 gas
Hanseniasporauvarum (Kloeckeraapiculata)
Producesestertaint(ethylacetate)
Producehighlevelofaceticacid
volatileacidity(VA)
Zygosaccharomyces spp.
Turbidity,sedimentformation,offcolors
Secondaryfermentationinbottle
Producehighlevelofaceticacid VA
Saccharomycodesludwigii
Produceshighconc.ofacetaldehyde
Producesflocculentmassesaschunks
Cancausewinetobecomeslimy

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WineMicrobiology
HowdoWineMicroorganismsSpoilWine?
Yeast
Brettanomyces spp.
Changetheodorandflavorofwine
Spoilcellarandbottledwineby
producinghaze,turbidity,CO2,&VA
Aromas:horseblanket,barnyard,wet
dog,leathery,medicinal,bandaid
Candidaspp. and Pichiaspp.
Formyeastfilmonwinesurface
Produceaceticacid&ethylacetate
Schizosaccharomycespombe
Secondaryfermentationinbottle
Sedimentformation

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WineMicrobiology
HowdoWineMicroorganismsSpoilWine?
Bacteria
AceticAcidBacteria
Acetobacter spp.
Aceticacid(vinegar)
Acetaldehyde(bruisedapple,green,
vegetative)
EthylAcetate(nailpolishremover)

Gluconobacteroxydans
Formationofaceticacid,acetaldehyde,
andethylacetate

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WineMicrobiology
HowdoWineMicroorganismsSpoilWine?
Bacteria
LacticAcidBacteria
Lactobacillus spp.
HighlevelsofVA

Mousytaint
Diacetyl
Acrolein
Degradestartaricacid
Ethylcarbamate precursors
Biogenicamines

Pediococcusspp.
Polysaccharides increaseviscosity
(ropiness)
Diacetyl
Acrolein
Biogenicamines

Oenococcusoeni
Ethylcarbamate precursors
VA

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Biogenicamines

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WineMicrobiology
HowdoWineMicroorganismsSpoilWine?
Mold
Penicilliumspp.,Trichodermaspp.,
Cladosporiumspp.,Fusarium spp.
TCA/TBAtaint Moldforms
TCA/TBAtaintinpresenceof
phenolsandchlorineorbromine
Atlowconcentrations,dullswine
flavorsandaromas
Athighconcentrations,makeswine
smellbad(moldy,musty,wet
cardboard)
FormedfromPhenols+Chlorineor
Bromine+Mold
Prevention highlevelofsanitation
toremovemold,getridofchlorine
&bromine

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WineMicrobiology
HowdoWineMicroorganismsSpoilWine?
Mold

Penicilliumspp.,Aspergillusspp.
Producehigheralcohols(fuselalcohol)
Produceodorssuchasmusty,mushroom
Mycotoxins (e.g.Ochratoxin A)

Botrytiscinerea,Aspergillusspp.,
Rhizopus nigricans
Produceglycerol carbonsourcefor
aceticacidbacteria

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WineMicrobiology
Prevention&ManagementofWineSpoilageMicrobes
Methods/practicestomanageorpreventwinespoilagemicrobes:
Sanitation
Enologicalpractices

Removewinesfromheavylees
Filtration
Maintaintankandbarrelheadspace
Alcoholicandmalolactic fermentationmanagement
Temperatureandhumiditycontrol
Maintainproperstaffinglevels

Viticultural practices
Grapequality healthygrapes
Transportofgrapestowinery cleanequipment,lowambient
temperature

WinePreservation
Sulfurdioxide
Velcorin
Lysozyme

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What is Sanitation?

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WhatisSanitation?

Sanitation:Practiceofenhancingproductqualityandshelflifeby
suppressingmicrobialgrowth.

Disinfection:Completedestructionofspecificmicroorganisms
(targetedelimination).

Sterilization:Thecompleteeliminationordestructionofallforms
ofmicrobiallife.

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WhatisSanitation?
WhatisCleaningandSanitizing?Aretheythesamething?
AThreeStepProcess:
Cleaning
Removalofvisibledebrisorsoils(wine,juice,tartrates,fruit)
eithermanually(scrubbing)ormechanically(spraying).
Usedetergentsincleanerstopenetrate,dislodge,andcarry
awaydirtanddebris.

Rinsing
Removalofresidualdebrisandcleaningchemicals.

Sanitizing
Onceequipmentisvisiblyclean,asanitizercanbeusedto
killthemicroorganismspresent.
Sanitizersbythemselvescannotbeusedtoeffectivelyremove
dirt,debris,andsoils.

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WhatisSanitation?
MajorFactorsInfluencingEffectivenessofCleaningandSanitizing
Time:
Increasedcleanercontacttime,greateramountofsoilremoved.
Increasedsanitizercontacttime,greatermicrobekillingpower.

Temperature:
Typicallyincreasedtemperatureleadstoimprovedcleaningpower.

ChemicalConcentrations:
Chemicalconcentrationsvarydependinguponthechemicalused,typeof
organicsoil,andtheequipmenttobecleaned.
Usingconcentratedformofcleaner/sanitizerisnotnecessarilybetter.
Concentrationnormallyreducedastimeandtemperatureareincreased.

MechanicalForce:
Simplehandscrubbingwithbrushtomechanicalscrubbing.
Mechanicalforceaidsinsoilremovalandtypicallyreducestime,temperature,
andconcentrationrequirements.

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Effects of Poor Sanitation

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EffectsofPoorSanitation
Presenceoffruit,must,juice,skins,seeds,orwineresidueserveas
sitesforaccumulationandgrowthofmicroorganisms.
Growthofmicroorganismscanleadtodeteriorationandspoilage
ofwineproducts.
MoldgrowthcanleadtoTCA
Brettanomyces growthcanspoilwines
Bacteriagrowthcanspoilwines(VA)
Microbecrosscontamination.
Effectivecleaningandsanitationpreventsmicrobesfrom
accumulatingandgrowing.

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SituationalExamples:
LinkBetweenPoorSanitationPractices&PoorWineQuality
CrushEquipmentExample
HighlevelsofLactobacillus inwine,dramaticspikeinVA,dropin
malicacid,andstuckfermentations.
HighlevelsofLactobacillus oncrushequipment.

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SituationalExamples:
LinkBetweenPoorSanitationPractices&PoorWineQuality
WineAgeingExamples
Example#1:
HighlevelsofBrettanomyces cellsand4EP/4EGincellaredwines.
HighlevelsofBrettanomyces cellsinwoodfrombarrels.

Example#2:
HighlevelsofBrettanomyces cellsand4EP/4EGincellaredwines.
Brettanomycescellsdetectedoncellarequipment.

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SituationalExamples:
LinkBetweenPoorSanitationPractices&PoorWineQuality
BottlingExamples
Example#1:
HighlevelsofBrettanomyces cellsbottledwines filteredduringbottling.
Brettanomyces cellsdetectedinhardlineassociatedwithbottling.

Example#2:
Zygosaccharomyces,Saccharomyces,andAcetobacter detectedinbottledproducts.
Samemicrobesdetectedonbottlingequipment.

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Selecting Cleaners for the Winemaking


Process

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SelectingCleanersfortheWinemakingProcess
CommonlyUsedCleaners
AcidCleaners(PhosphoricAcid)
CausticCleaners(NaOH orKOH)
HighPressureSprayCleaner
SodiumPercarbonate w/H2O2
Trisodium Phosphate(TSP)

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SelectingCleanersfortheWinemakingProcess
Advantages&DisadvantagesofCleaners
Cleaner
AcidCleaners
(PhosphoricAcid)

Advantages

Disadvantages

Reducemineraldeposits hardwater Notaseffectiveat


removingsoils
stains&rust
Canbecorrosiveto
Softenwater
stainlesssteel
Environmentalconcerns

CausticCleaners Effectivelycleansheavilysoiled
(NaOH orKOH)
equipment(removes tartrates)
Effectivelyremoveswineorjuice
stains
Hassomeantimicrobialactivity
Canbereusedformultipletanks
Easytorinseafteruse
KOHisenvironmentallyfriendly

Canposeasafetyrisk
Canbecorrosiveon
stainless steelabove
recommended
concentrations

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SelectingCleanersfortheWinemakingProcess
Advantages&DisadvantagesofCleaners
Cleaner

Advantages

Disadvantages

HighPressure
SprayCleaner

Effectivelycleansheavilysoiled
equipment(including tartrates)

Doesnoteffectivelyremove
longtermstains
Aerosilizes microorganisms
Cancauseequipment
malfunctionsbypene
trating into sensitiveareas

Sodium
Percarbonate
withH2O2

Notaseffectiveonheavily
AlsocalledOxygenBleach
soiledequipment
Effectivelycleansandbleacheslightly
soiledequipment
Dissolvesrapidlyinwater
Rinsesoffeasily
Consideredtobeastrongfungicide

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SelectingCleanersfortheWinemakingProcess
Advantages&DisadvantagesofCleaners
Cleaner
Trisodium
Phosphate(TSP)

Advantages
Effectivelycleanslightlysoiled
equipment
Phosphates helpsoftenwater

Disadvantages
Lowsolubilityinambient
temperaturewater
Not aneffectivedestaining
agent
Notconsideredtobe
environmentally friendly

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Selecting Sanitizers for the Winemaking


Process

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SelectingSanitizersfortheWinemakingProcess
CommonlyUsedSanitizers
Chlorine
Chlorinedioxide
Hotwater/Steam
Iodophors (Iodine)
Ozone
Peroxyacetic acid
Quaternaryammoniumcompounds(QUATS)
SulfurDioxide(acidulated)
CitricAcid(?)

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SelectingSanitizersfortheWinemakingProcess
Advantages&DisadvantagesofSanitizers
Sanitizer
Chlorine

ChlorineDioxide

Advantages

Disadvantages

Veryeffective;usedinwine
industryfor decades

HighriskofTCAformation
notrecommendedina
winerysetting

Strongestantimicrobialaction
broadspectrum
EffectiveoverawidepHrange(2to
10)
Breaksdownandinhibitsbiofilms
Biocidal towardssporeformers
Deodorizer
Doesnotformcarcinogenic
compounds
Environmentallyfriendly
TCAformation (?)

Requireslargeupfront
capitalinvestment cheap
chemicals
Mustbegeneratedonsite
SusceptibletoheatandUV
lightdecomposition
Corrosivetosoftmetals:
copper,brass,andaluminum
Vaporsfromactivationstep
canbeasafetyhazard

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SelectingSanitizersfortheWinemakingProcess
Advantages&DisadvantagesofSanitizers
Sanitizer

Advantages

Hot Water/Steam

Strongandbroad antimicrobial
activity
Sanitizescracksandnoncontact
surfacesviaheatconduction
Improveseffectivenessofsoil
removal

Iodophors (Iodine) Strongantimicrobialactivity


includingsporeformers
Effectiveagainstbroadrangeof
microbes
Loweruselevelthanchlorine

Disadvantages
Expensive energycostsand
maintenance
Highcapitalcoststoinstall
Requiresmoretimetosanitize
surfacethanchemicals
Canencouragebiofilm
formation
Containsiodine anisole
formationrisk
Safetyrisk
MosteffectiveinnarrowpH
range(45)
Canstainsurfaces

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SelectingSanitizersfortheWinemakingProcess
Advantages&DisadvantagesofSanitizers
Sanitizer
Ozone

Advantages
Powerfulantimicrobialactivity
Requiresverylittlecontacttime
PotencynotaffectedbypHor
temperature
Breaks downbiofilms
Deodorizer
Environmentallyfriendly

Disadvantages
Poorsolubility inwater
Veryshorthalflife
Corrosivetoequipment
Reactswithanyorganicmaterial
Canbetoxic
Userequiressafety
monitoring
Candamagetissueinlungs
Cannotbestoredforlateruse
Initialcostforequipment
Lackoflongresidualactivityisa
significantdrawbackforusein
largedistributionsystems

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SelectingSanitizersfortheWinemakingProcess
Advantages&DisadvantagesofSanitizers
Sanitizer

Advantages

Peroxyacetic Acid Strongantimicrobialactivity


(PAA)
overwidepHrange(1to9)
Effectiveover awide
temperaturerange
Fastantimicrobialactivity
Effectiveagainstbroadrangeof
microbes
Degradesbiofilms
Nopostrinserequired
Environmentallyfriendly

Disadvantages
Oneofthemostexpensive
chemicalsanitizers
Concentrateformhasstrong
odor&istoxic
Rapidlydecomposesinpresence
ofmetals
Corrosivetometalssuchas
brass,copper,iron,mildsteel,
andaluminum
Breaksdownmostplastics Use
Teflon
Moreexpensivethanmost
sanitizers

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SelectingSanitizersfortheWinemakingProcess
Advantages&DisadvantagesofSanitizers
Sanitizer

Advantages

Quaternary
Ammonium
Compounds
(QUATS)

StableoverawiderangeofpH
&temperatures
Longtermresidualaction
Goodprotectionagainstmold
growth
Doesnotrequirerinsing
Noncorrosive

Lack ofbroadspectrumactivity
againstmicrobes(especially
Gramnegativebacteria)
InactivatedbysaltsandlowpH
Leavesresidualfilm bestfor
external&nonfoodsurfaces
Moderatelytoxicatuse
solutions

Longshelflife
Lowcosttouse

Antimicrobialactivityisonly
effectiveatlowpH(34)
Doesnothaveabroadspectrum
ofantimicrobialactivity
Verycorrosiveinpresenceof
moisture

SulfurDioxide
(Acidulated)

Disadvantages

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Recommended Sanitation Methods for


Equipment & Infrastructure

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RecommendedSanitationMethodsfor Equipment
andInfrastructure
VariablesAffectingSanitationMethods
Ageoffacility.
Sizeoffacility.
Infrastructureconstraints(i.e.sanitarydesign,enclosedareas).
Environmentalconstraints(i.e.airquality,waterquality).
Availabilityoflabor.
Productionconstraints(i.e.throughout).
Qualityfocus.
Managementcommitment.
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RecommendedSanitationMethodsfor Equipment
andInfrastructure
KeepOptionsOpenforCleanerandSanitizerUse
Somecleanersarebetterforheavilysoiledequipment.
Certainsanitizersaremoreeffectiveagainstparticularmicrobes
thanothers.
Removingwinesmicrobesfromoakcooperagemayrequirea
differentsanitizerthanfrombottlingline.
Removingbiofilms willrequireusingdifferentsanitationregime.
Periodicallychangeoutorcontinuallyalternatesanitizers.
Equipmentwillgenerallyrequiredifferentcleanersandsanitizers
thaninfrastructure.

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RecommendedSanitationMethodsfor Equipment
andInfrastructure
CriteriaforSelectingCleanersandSanitizers

Amountoforganicdebris.
Typeoforganicdebris(loose,caked,stain,biofilm).
Typesofmicrobespresent(ifknown).
Typeofequipment.
Typeofmaterialbeingcleanedorsanitized(stainlesssteel,
wood,plastic)
Costofcleanerorsanitizer.
Efficacyofcleanerorsanitizer.
Environmentalimpact(positive,negative,neutral).
Waterquality.

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RecommendedSanitationMethodsfor Equipment
andInfrastructure
Crush/CellarEquipment
Equipmentincludescrushpits,destemmers,presses,tanks,
pumps,hoses,hardlines,fittings,etc.
Cleanandsanitizeaftereachuse.
Sanitizebeforeeachuse:
Dependsonsanitizerholdtimes.

Deepcleaning/sanitizing:
Semiannuallyorannually.
PreandPostharvest.

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RecommendedSanitationMethodsfor Equipment
andInfrastructure
RoutineCleaningandSanitationofOakCooperage
Cleanwithhotwaterunderpressureorsteam.
Rinsewithambienttemperaturewatertocooldownoak.
Sanitizeinteriorsurfacewithozonated water.
Afteremptying,fillwitheitherofthefollowing:
Wine ideallyhighalcohol,lowpH,highSO2 wine.
SO2/citricacidsolution.
SO2 gas immediatelysealupafteradding.

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RecommendedSanitationMethodsfor Equipment
andInfrastructure
DeepCleaningandSanitationofOakCooperage
Cleanwithwarmsolutionofsodiumpercarbonate for24hours.
Rinsewithwaterandneutralizewithcitricacid.
Rinsewithambienttemperaturewatertocooldownoak.
Sanitizeinteriorsurfacewithozonated water.
Afteremptying,fillwitheitherofthefollowing:
Wine ideallyhighalcohol,lowpH,highSO2 wine.
SO2/citricacidsolution.
SO2 gas immediatelysealupafteradding.

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RecommendedSanitationMethodsfor Equipment
andInfrastructure
BottlingLineEquipment
Cleanandsanitizedailyatshutdown:
Clean.
Rinsewithambienttemperaturewater.
Sanitize.

Sanitizedailyatstartup:
Hotwater(180Cforatleast20minutes)or
Steam(condensate180Cforatleast20minutes).

Corkingsystem:
Corkerjaws soakedin70%ethanolovernight.
Corkhopper Routinelyspraywith70%ethanol.

Ensureintegrityoffiltersondailybasis.
Periodically(quarterly,semiannually,annually)conductdeep
cleaning/sanitizingofbottlingequipment.
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RecommendedSanitationMethodsfor Equipment
andInfrastructure
VentilationEquipment
Regularlyinspect(i.e.quarterly)formicrobialgrowth(mold,yeast,
bacteria).
Cleanandsanitizeifsignificantgrowthispresent.
Annuallyconductthoroughcleaningandsanitizingofequipment.

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RecommendedSanitationMethodsfor Equipment
andInfrastructure
Infrastructure
Infrastructureincludeswalls,floors,ceiling,supportstructures,drains,sumps,etc.
Regularlyinspect(i.e.quarterly)formicrobialgrowth(mold,yeast,bacteria).
Cleanandsanitizeifsignificantgrowthispresent.
Annuallyconductthoroughcleaningandsanitizing.
Drainsandsumps:
Cleanaccessibleportions.
Flushwithcleaner(approveddraincleaner)andsanitizer.

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Cutting Edge Cleaning/Sanitizing


Practices for Wineries

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CuttingEdgeCleaning/SanitizingPracticesforWineries
AssessSanitaryDesignofEquipment&Infrastructure
Equipment:

Accessibleforinspection,maintenance,cleaningandsanitation.
316stainlesssteelforproductcontactequipment.
Selfdraining.
Nonporous,freeofcracksandcrevices.
Roundedcorners.
Smoothwelds.
Nothreadedfittings.

Infrastructure:
Accessibleforcleaningandsanitation.
Smooth,sealed,freedrainingfloors nostandingorpoolingliquids.
Usenonpaintableandnoncorrosiveconstructionmaterials.
Airblowingintoprocessingareamustbefiltered.
Waterusedforwineproductionmustbefiltered.
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CuttingEdgeCleaning/SanitizingPracticesforWineries
Foam/GelContainingCleaners
Technology

Applications

Foamisproducedthrough Foam/gel equipmentand


infrastructure:
theintroductionofairinto
Crush
adetergentsolutionasitis
Cellar
sprayedontothesurface
Bottling
tobecleaned.

Advantages/
Disadvantages
Cleanerremainsindirect
contactw/surfaceforlong
periodsoftime
Canbeusedon
horizontalorvertical
surfaces
Doesnotpenetrate
sensitiveareas
Requirespurchaseof
equipment
Chemicalscanbe
expensive

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CuttingEdgeCleaning/SanitizingPracticesforWineries
UltravioletLight
Technology
Ultraviolet lightsthat
producehighenergy
radiation(between100
and280nm).
Germicidalshortwaves
thatcauseirreversible
damagetocellularDNA.

Applications
Reduce microbialloads:
Cellar&bottlingequip.
Oakcooperageafter
cleaning.
Facilityinfrastructure.
Atmosphere.
Water.
Wine.

Advantages/
Disadvantages
Reduce chemicalandwater
use.
Potentialenergysavings
forventilationsystems.
Safety issues.
Costforequipmentand
maintenance.
Wine:
ReplaceSO2 oxidation
potential.
Impactonsensory
profile.

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CuttingEdgeCleaning/SanitizingPracticesforWineries
DryIceBlasting
Technology

Applications

Surface treatmentfor
Particles ofsolidcarbon
barrelinteriors:
dioxide(dryice)are
Microberemoval
propelledathighspeedsto
Rejuvenateoak
stripandcleansurfaces.
character.
Otherequipmentand
infrastructuresurfaces

Advantages/
Disadvantages
Removes microbialload,
tartrate crystals,andwine
residues.
Environmentallyfriendly:
Reduces/eliminates
chemicaluse.
Savesonwateruse.

Extendbarrellife.
Microbesembeddedin
wood(pores,cracks)may
notberemovedor
destroyed.

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CuttingEdgeCleaning/SanitizingPracticesforWineries
Microwave Sterilization
Technology
Microwaves arenon
ionizingradiationwaves
thatexcitewater
moleculesatexactlythe
rightwavelength toheat
themup.
Hyperthermic conditions
killmicrobesbyheating
watermoleculesin cells.

Applications
Use incombination
withcleaningsolutions
tocleanandsterilize
barrelinteriors.
Waterandwastewater
treatment.
Wine?

Advantages/
Disadvantages
Sterilizewineryprocess
waterandwastewater.
Requireswater.
High capitalcostfor
equipmentand
infrastructure.

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CuttingEdgeCleaning/SanitizingPracticesforWineries
HighPowerUltrasonics
Technology

Applications

Ultrasonic soundwaves
causegasbubblesin
liquidstoexpand and
contractthousandsof
timespersecondcausing
themimplode(cavitate).
Cavitation leads tohigh
temps,pressures,and strong
shearforces.
Resultsinstrongagitation
actionforcleaning,
accelerateschemical
reactions,andbreakdown
ofmolecularstructures.

Interior barrelcleaningand
sanitationincluding
eliminationofmicrobes
deepintowood.
Cleaningandsanitationof
oakadjuncts.
Defoaming productson
bottlingline.
Increasefermentationrates.
Sterilizewatersupply.
Sterilizewine?

Advantages/
Disadvantages
Simultaneous cleaning and
sanitation.
Recyclewaterusedfor
barrels.
Eliminateschemicaluse.
Extendbarrellife.
Costofequipmentor
service.
Processingtime.

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Methods to Monitor Effectiveness of


Cleaning & Sanitation Practices
An ounce of prevention is worth a pound of cure
Benjamin Franklin

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MethodstoMonitorEffectivenessofCleaning&
SanitationPractices
WaystoMeasureEffectivenessofCleaning&Sanitation
SensoryInspection
Visual
Touch
Smell
Taste
AdenosineTriphosphate (ATP)Test(also
calledLuminometer Test)
AsepticSwabbing&CulturingofMicrobes

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MethodstoMonitorEffectivenessofCleaning&
SanitationPractices
SensoryInspection
Providesoverallpictureofcleaning&sanitationeffectiveness
Measurecleaning&sanitationbyusing4of5senses:
Visual Doesitlookclean?
Smell Doesitsmellclean?
Touch Doesitfeelclean(smooth)?
Taste
Howcanonemeasuresensoryinspections?
45points Satisfactory(noaction)
23points NeedsImprovement(actionASAP)
01points Unsatisfactory(immediateaction)

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MethodstoMonitorEffectivenessofCleaning&
SanitationPractices
AdenosineTriphosphate (ATP)Test
Realtimemethodtomeasurelevelofcleanlinessonsurface
WhatisATP?
AdenosineTriphosphate
Chemicalfoundinallanimal,plant,andmicrobialcells,
andinproductresidues

WhatisATPtest?
Lighttest measurescleanlinessofsurfaces
ATPismeasuredusinganenzymaticreaction,which
occursinthetailoffirefliestoproducelight
LightemittedisproportionaltoamountofATPpresent

WhataremeasurementsforATPtest?
RelativeLightUnits(RLU)

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MethodstoMonitorEffectivenessofCleaning&
SanitationPractices
AsepticSwabbing&CulturingofMicrobes
Swabmethodtomeasurelevelofsanitationonsurface
Howdoesmicrobialswabbingtestwork?
Applysterileswabover4in.x4in.surfacearea
Swabistransferredtosterileliquid&shaken
Liquidisputonplateswhichallowmicrobestogrow

Whatareresultsofmicrobialswabbingtestandwhatdothey
mean?
Winespoilagemicrobes(Brettanomyces,Pediococcus,
Acetobacter,Lactobacillus)=Surface/equipmentmustbe
recleanedandresanitizedimmediately
Othermicrobes=Indicatessanitationcompromised;sourceof
contaminationshouldbefound

Indicatorofeffectivenessofsanitationpractices/techniques.

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MethodstoMonitorEffectivenessofCleaning&
SanitationPractices
ATPResultsDoNotDirectlyCorrelatewithMicrobialCounts
ThereisnodirectcorrelationbetweenATPresultsandmicrobialcounts:
HighRLUreadingandlowmicrobecounts.
LowRLUreadingandhighmicrobecounts.

ATPreadingscanbecombinationofbenignwineresidueandspoilage
microbes.
ATPamountscanvarywidelybetweenmicrobes.
Effectivesanitationmonitoringtypicallyincorporatesvisual,ATP,and
microbialswabbing.

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MethodstoMonitorEffectivenessofCleaning&
SanitationPractices
ReferenceGuideforSanitationMonitoringMethods
Method

Advantages

Disadvantages

Recommended
Frequency*
Eachuse.

Visual
Monitoring

Canbecompletedquickly.
Allemployeescanperform.
Nocost.

Very subjective.
Not reliable to determine
sanitarycondition.

Adenosine
Triphosphate
(ATP)

Resultsarequantitative.
Immediateresults.

Doesnotindicatetypeof Daily/Weekly
microbespresent.
DifficulttocorrelateATP
& microbialswabbing.

Microbial
Swabbing/
Culturing

Canquantifynumberandtypeof
microbespresent.
Canbeusedtotrendsanitary
conditionoffacility/equipment.
Proactivemethodtoimprove
winequality.

Resultsnotavailablefor
4to7days.

Weekly/
Monthly/
Quarterly

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* Variesbyequipment/facilitysize/risk

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Establishing Microbiological Criteria


For Winery Sanitation Practices

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EstablishingMicrobiologicalCriteriaforWinery
SanitationPractices
Developahistoricaldatabaseofsanitationdata(ATPtestsand/or
microbiologicalswabbing/culturingtests)foryourfacility.
Usecombinationofthisdata,winescienceliterature,and
industryexpertstodefineacceptablelimits(controllimits)for:
ATP
Microbialloads

Usethesecontrollimitstoensureeffectivenessofcleaningand
sanitationpractices.
Establishcorrectiveactionpolicyifcontrollimit(s)isexceeded:
Additionalcleaningand/orsanitizing
Rootcauseanalysis

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Requirements for a Successful & Sustainable


Winery Sanitation Program

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RequirementsforaSuccessful&SustainableWinery
SanitationProgram

Managementcommitment
Knowledgeofprocess,people,regulations,equipment,budget,andcustomer
expectations
Definebaselinesanitarystateoffacility:
Reviewcurrentwrittenandverbalsanitationpractices.
Identifypotentialsanitarydesignproblems.
Indepthsanitationassessmentofequipment,infrastructure,&environment.

Developandimplementaneffectiveprogram:
Findcleanersandsanitizersthathavehighefficacy,arecosteffective,&minimallyimpact
environment.
Sanitationstandardoperatingprocedures(SSOPs).
Mastercleaningandsanitationschedule.
GoodManufacturingPractices(GMP)program
Sanitationspecificationsforequipmentandinfrastructure.

Sanitationmaintenanceprogram:
Routinemonitoringofsanitationeffectiveness.
Continuousprocessimprovement.
Documentationreviews.

Ongoingsanitationtrainingandeducation

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Benefits of a Successful & Sustainable


Winery Sanitation Program

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BenefitsofaSuccessful&SustainableWinery
SanitationProgram
Improvedwinequality.
Increasedproductivityandoperatingefficiency.
Greaterconsistencyofproducts.
Reducedproductdowngrades.
Reducedrework.
Reducedproductioncosts.
Reducedholdtimesforfinishedproducts.
Reducedenvironmentalimpact.
Achievecreative/innovativeedgeovercompetitors.
Employeeempowermentconfidence,performance,andcommunication.

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Winery Sanitation
A critical component of wine quality

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