Professional Documents
Culture Documents
Bevtrac: Practical Winery Sanitation
Bevtrac: Practical Winery Sanitation
Crushequipment
Barrels
Tanks
Winehosesandtransferlines
Generalwineryequipment
Bottlingline
Obtainingcontaminatedwinefromotherwineries
Environmentalcontamination atmosphere,water
Sanitationpractices
pH
Oxygen
Nutrients
Winestoragetemperature
Moistureavailability
Lagphasetime
Generationtime
Gluconobacteroxydans
Formationofaceticacid,acetaldehyde,
andethylacetate
Mousytaint
Diacetyl
Acrolein
Degradestartaricacid
Ethylcarbamate precursors
Biogenicamines
Pediococcusspp.
Polysaccharides increaseviscosity
(ropiness)
Diacetyl
Acrolein
Biogenicamines
Oenococcusoeni
Ethylcarbamate precursors
VA
Penicilliumspp.,Aspergillusspp.
Producehigheralcohols(fuselalcohol)
Produceodorssuchasmusty,mushroom
Mycotoxins (e.g.Ochratoxin A)
Botrytiscinerea,Aspergillusspp.,
Rhizopus nigricans
Produceglycerol carbonsourcefor
aceticacidbacteria
Removewinesfromheavylees
Filtration
Maintaintankandbarrelheadspace
Alcoholicandmalolactic fermentationmanagement
Temperatureandhumiditycontrol
Maintainproperstaffinglevels
Viticultural practices
Grapequality healthygrapes
Transportofgrapestowinery cleanequipment,lowambient
temperature
WinePreservation
Sulfurdioxide
Velcorin
Lysozyme
What is Sanitation?
Sanitation:Practiceofenhancingproductqualityandshelflifeby
suppressingmicrobialgrowth.
Disinfection:Completedestructionofspecificmicroorganisms
(targetedelimination).
Sterilization:Thecompleteeliminationordestructionofallforms
ofmicrobiallife.
Rinsing
Removalofresidualdebrisandcleaningchemicals.
Sanitizing
Onceequipmentisvisiblyclean,asanitizercanbeusedto
killthemicroorganismspresent.
Sanitizersbythemselvescannotbeusedtoeffectivelyremove
dirt,debris,andsoils.
Temperature:
Typicallyincreasedtemperatureleadstoimprovedcleaningpower.
ChemicalConcentrations:
Chemicalconcentrationsvarydependinguponthechemicalused,typeof
organicsoil,andtheequipmenttobecleaned.
Usingconcentratedformofcleaner/sanitizerisnotnecessarilybetter.
Concentrationnormallyreducedastimeandtemperatureareincreased.
MechanicalForce:
Simplehandscrubbingwithbrushtomechanicalscrubbing.
Mechanicalforceaidsinsoilremovalandtypicallyreducestime,temperature,
andconcentrationrequirements.
Example#2:
HighlevelsofBrettanomyces cellsand4EP/4EGincellaredwines.
Brettanomycescellsdetectedoncellarequipment.
Example#2:
Zygosaccharomyces,Saccharomyces,andAcetobacter detectedinbottledproducts.
Samemicrobesdetectedonbottlingequipment.
Advantages
Disadvantages
CausticCleaners Effectivelycleansheavilysoiled
(NaOH orKOH)
equipment(removes tartrates)
Effectivelyremoveswineorjuice
stains
Hassomeantimicrobialactivity
Canbereusedformultipletanks
Easytorinseafteruse
KOHisenvironmentallyfriendly
Canposeasafetyrisk
Canbecorrosiveon
stainless steelabove
recommended
concentrations
Advantages
Disadvantages
HighPressure
SprayCleaner
Effectivelycleansheavilysoiled
equipment(including tartrates)
Doesnoteffectivelyremove
longtermstains
Aerosilizes microorganisms
Cancauseequipment
malfunctionsbypene
trating into sensitiveareas
Sodium
Percarbonate
withH2O2
Notaseffectiveonheavily
AlsocalledOxygenBleach
soiledequipment
Effectivelycleansandbleacheslightly
soiledequipment
Dissolvesrapidlyinwater
Rinsesoffeasily
Consideredtobeastrongfungicide
Advantages
Effectivelycleanslightlysoiled
equipment
Phosphates helpsoftenwater
Disadvantages
Lowsolubilityinambient
temperaturewater
Not aneffectivedestaining
agent
Notconsideredtobe
environmentally friendly
ChlorineDioxide
Advantages
Disadvantages
Veryeffective;usedinwine
industryfor decades
HighriskofTCAformation
notrecommendedina
winerysetting
Strongestantimicrobialaction
broadspectrum
EffectiveoverawidepHrange(2to
10)
Breaksdownandinhibitsbiofilms
Biocidal towardssporeformers
Deodorizer
Doesnotformcarcinogenic
compounds
Environmentallyfriendly
TCAformation (?)
Requireslargeupfront
capitalinvestment cheap
chemicals
Mustbegeneratedonsite
SusceptibletoheatandUV
lightdecomposition
Corrosivetosoftmetals:
copper,brass,andaluminum
Vaporsfromactivationstep
canbeasafetyhazard
Advantages
Hot Water/Steam
Strongandbroad antimicrobial
activity
Sanitizescracksandnoncontact
surfacesviaheatconduction
Improveseffectivenessofsoil
removal
Disadvantages
Expensive energycostsand
maintenance
Highcapitalcoststoinstall
Requiresmoretimetosanitize
surfacethanchemicals
Canencouragebiofilm
formation
Containsiodine anisole
formationrisk
Safetyrisk
MosteffectiveinnarrowpH
range(45)
Canstainsurfaces
Advantages
Powerfulantimicrobialactivity
Requiresverylittlecontacttime
PotencynotaffectedbypHor
temperature
Breaks downbiofilms
Deodorizer
Environmentallyfriendly
Disadvantages
Poorsolubility inwater
Veryshorthalflife
Corrosivetoequipment
Reactswithanyorganicmaterial
Canbetoxic
Userequiressafety
monitoring
Candamagetissueinlungs
Cannotbestoredforlateruse
Initialcostforequipment
Lackoflongresidualactivityisa
significantdrawbackforusein
largedistributionsystems
Advantages
Disadvantages
Oneofthemostexpensive
chemicalsanitizers
Concentrateformhasstrong
odor&istoxic
Rapidlydecomposesinpresence
ofmetals
Corrosivetometalssuchas
brass,copper,iron,mildsteel,
andaluminum
Breaksdownmostplastics Use
Teflon
Moreexpensivethanmost
sanitizers
Advantages
Quaternary
Ammonium
Compounds
(QUATS)
StableoverawiderangeofpH
&temperatures
Longtermresidualaction
Goodprotectionagainstmold
growth
Doesnotrequirerinsing
Noncorrosive
Lack ofbroadspectrumactivity
againstmicrobes(especially
Gramnegativebacteria)
InactivatedbysaltsandlowpH
Leavesresidualfilm bestfor
external&nonfoodsurfaces
Moderatelytoxicatuse
solutions
Longshelflife
Lowcosttouse
Antimicrobialactivityisonly
effectiveatlowpH(34)
Doesnothaveabroadspectrum
ofantimicrobialactivity
Verycorrosiveinpresenceof
moisture
SulfurDioxide
(Acidulated)
Disadvantages
Lab to You
Amountoforganicdebris.
Typeoforganicdebris(loose,caked,stain,biofilm).
Typesofmicrobespresent(ifknown).
Typeofequipment.
Typeofmaterialbeingcleanedorsanitized(stainlesssteel,
wood,plastic)
Costofcleanerorsanitizer.
Efficacyofcleanerorsanitizer.
Environmentalimpact(positive,negative,neutral).
Waterquality.
Deepcleaning/sanitizing:
Semiannuallyorannually.
PreandPostharvest.
Sanitizedailyatstartup:
Hotwater(180Cforatleast20minutes)or
Steam(condensate180Cforatleast20minutes).
Corkingsystem:
Corkerjaws soakedin70%ethanolovernight.
Corkhopper Routinelyspraywith70%ethanol.
Ensureintegrityoffiltersondailybasis.
Periodically(quarterly,semiannually,annually)conductdeep
cleaning/sanitizingofbottlingequipment.
We
Bring the Wine Lab to You
Accessibleforinspection,maintenance,cleaningandsanitation.
316stainlesssteelforproductcontactequipment.
Selfdraining.
Nonporous,freeofcracksandcrevices.
Roundedcorners.
Smoothwelds.
Nothreadedfittings.
Infrastructure:
Accessibleforcleaningandsanitation.
Smooth,sealed,freedrainingfloors nostandingorpoolingliquids.
Usenonpaintableandnoncorrosiveconstructionmaterials.
Airblowingintoprocessingareamustbefiltered.
Waterusedforwineproductionmustbefiltered.
We
Adequatelighting
Bring the Wine Lab to You
Applications
Advantages/
Disadvantages
Cleanerremainsindirect
contactw/surfaceforlong
periodsoftime
Canbeusedon
horizontalorvertical
surfaces
Doesnotpenetrate
sensitiveareas
Requirespurchaseof
equipment
Chemicalscanbe
expensive
Applications
Reduce microbialloads:
Cellar&bottlingequip.
Oakcooperageafter
cleaning.
Facilityinfrastructure.
Atmosphere.
Water.
Wine.
Advantages/
Disadvantages
Reduce chemicalandwater
use.
Potentialenergysavings
forventilationsystems.
Safety issues.
Costforequipmentand
maintenance.
Wine:
ReplaceSO2 oxidation
potential.
Impactonsensory
profile.
Applications
Surface treatmentfor
Particles ofsolidcarbon
barrelinteriors:
dioxide(dryice)are
Microberemoval
propelledathighspeedsto
Rejuvenateoak
stripandcleansurfaces.
character.
Otherequipmentand
infrastructuresurfaces
Advantages/
Disadvantages
Removes microbialload,
tartrate crystals,andwine
residues.
Environmentallyfriendly:
Reduces/eliminates
chemicaluse.
Savesonwateruse.
Extendbarrellife.
Microbesembeddedin
wood(pores,cracks)may
notberemovedor
destroyed.
Applications
Use incombination
withcleaningsolutions
tocleanandsterilize
barrelinteriors.
Waterandwastewater
treatment.
Wine?
Advantages/
Disadvantages
Sterilizewineryprocess
waterandwastewater.
Requireswater.
High capitalcostfor
equipmentand
infrastructure.
Applications
Ultrasonic soundwaves
causegasbubblesin
liquidstoexpand and
contractthousandsof
timespersecondcausing
themimplode(cavitate).
Cavitation leads tohigh
temps,pressures,and strong
shearforces.
Resultsinstrongagitation
actionforcleaning,
accelerateschemical
reactions,andbreakdown
ofmolecularstructures.
Interior barrelcleaningand
sanitationincluding
eliminationofmicrobes
deepintowood.
Cleaningandsanitationof
oakadjuncts.
Defoaming productson
bottlingline.
Increasefermentationrates.
Sterilizewatersupply.
Sterilizewine?
Advantages/
Disadvantages
Simultaneous cleaning and
sanitation.
Recyclewaterusedfor
barrels.
Eliminateschemicaluse.
Extendbarrellife.
Costofequipmentor
service.
Processingtime.
WhatisATPtest?
Lighttest measurescleanlinessofsurfaces
ATPismeasuredusinganenzymaticreaction,which
occursinthetailoffirefliestoproducelight
LightemittedisproportionaltoamountofATPpresent
WhataremeasurementsforATPtest?
RelativeLightUnits(RLU)
Whatareresultsofmicrobialswabbingtestandwhatdothey
mean?
Winespoilagemicrobes(Brettanomyces,Pediococcus,
Acetobacter,Lactobacillus)=Surface/equipmentmustbe
recleanedandresanitizedimmediately
Othermicrobes=Indicatessanitationcompromised;sourceof
contaminationshouldbefound
Indicatorofeffectivenessofsanitationpractices/techniques.
ATPreadingscanbecombinationofbenignwineresidueandspoilage
microbes.
ATPamountscanvarywidelybetweenmicrobes.
Effectivesanitationmonitoringtypicallyincorporatesvisual,ATP,and
microbialswabbing.
Advantages
Disadvantages
Recommended
Frequency*
Eachuse.
Visual
Monitoring
Canbecompletedquickly.
Allemployeescanperform.
Nocost.
Very subjective.
Not reliable to determine
sanitarycondition.
Adenosine
Triphosphate
(ATP)
Resultsarequantitative.
Immediateresults.
Doesnotindicatetypeof Daily/Weekly
microbespresent.
DifficulttocorrelateATP
& microbialswabbing.
Microbial
Swabbing/
Culturing
Canquantifynumberandtypeof
microbespresent.
Canbeusedtotrendsanitary
conditionoffacility/equipment.
Proactivemethodtoimprove
winequality.
Resultsnotavailablefor
4to7days.
Weekly/
Monthly/
Quarterly
* Variesbyequipment/facilitysize/risk
Usethesecontrollimitstoensureeffectivenessofcleaningand
sanitationpractices.
Establishcorrectiveactionpolicyifcontrollimit(s)isexceeded:
Additionalcleaningand/orsanitizing
Rootcauseanalysis
Managementcommitment
Knowledgeofprocess,people,regulations,equipment,budget,andcustomer
expectations
Definebaselinesanitarystateoffacility:
Reviewcurrentwrittenandverbalsanitationpractices.
Identifypotentialsanitarydesignproblems.
Indepthsanitationassessmentofequipment,infrastructure,&environment.
Developandimplementaneffectiveprogram:
Findcleanersandsanitizersthathavehighefficacy,arecosteffective,&minimallyimpact
environment.
Sanitationstandardoperatingprocedures(SSOPs).
Mastercleaningandsanitationschedule.
GoodManufacturingPractices(GMP)program
Sanitationspecificationsforequipmentandinfrastructure.
Sanitationmaintenanceprogram:
Routinemonitoringofsanitationeffectiveness.
Continuousprocessimprovement.
Documentationreviews.
Ongoingsanitationtrainingandeducation
Winery Sanitation
A critical component of wine quality