Professional Documents
Culture Documents
Column Intro
Column Intro
Column Intro
Methods
Leaves of tsaang gubat, Carmona retusa Merr., were collected from the botanical
garden in the roof deck of University of the Philippines College of Pharmacy. The
leaves were removed from the stems then were triturated or cut into smaller
segments before undergoing the extraction procedure. Solvent used for extraction
of the pigments in the leaves was ethanol and of technical grade quality. The leaves
were then macerated with the ethanol, 95%, and left for at least 65 hours before
the experiment proper. The hexane and ethyl acetate used as mobile phase were of
technical grade also.
The column was then packed using three different adsorbents with varying degrees
of absorptive power. Each layer then is separated by a piece of filter paper. Alumina,
10ml, is packed on the first layer, magnesium carbonate, 10ml, on the second and
lastly, 40ml of powdered sucrose. The column was then subjected to equilibration
by saturating it with the mobile phase before the introduction of the extract. The
extract, 5ml of which, was put into the top of the column and is washed with
enough mobile phase, preventing the layers to dry, letting the sample to travel
down the packed stationary phase. This was done until a part of the pigments
reached the end of the column. No pigments or compounds of the extract were
eluted.
References:
Skoog, D., West, D., Holler, J. and Crouch, S. (2014). Fundamentals of Analytical
Chemistry (9th ed.). Belmont, CA: Cengage Learning.