Biology___________________________________WAJA PPSMI MODULE 2008

SAMPLE 2 - OSMOSIS
1

A student carried out an experiment to study the effect of different concentrations

of sodium chloride solution on red blood cells within 30 minutes.

Sodium
chloride
+
Redblood
cells

TesttubeA

Microscope

Stopwatch

Diagram1(a)

Diagram1(b)
Diagram 1(a) shows the experiment set up by the student. Diagram 1(b) shows the red
blood cells under the microscope after being immersed in 0.15 M sodium chloride
solution (test tube A) for 30 minutes.
The experiments were repeated at different concentrations of sodium chloride, 0.30 M,
0.45 M and 0.60 M. Red blood cells were then immersed in test tube B (0.30 M), C (0.45
M) and D (0.60 M) respectively. The results are shown in Table 1.

Biology___________________________________WAJA PPSMI MODULE 2008

Test
Tube

Concentration of
sodium chloride
solution (M)

Red blood cells as seen under

the
microscope.

Number of crenated
red blood cells.

Crenated red blood

cell
Normal red blood
cell

A
0.3

0.45

0.60

TABLE 1

Biology___________________________________WAJA PPSMI MODULE 2008

(a) (i) Based on Table 1, state two observations on the relationship between the number
of crenated red blood cells and the concentration of sodium chloride solution.
1 _________________________________________________________________________
___________________________________________________________________________
2 _________________________________________________________________________
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[3 marks]
(ii) State the inference which corresponds to the observations in (a)(i).
1 __________________________________________________________________________
____________________________________________________________________________
2 __________________________________________________________________________
____________________________________________________________________________
[3 marks]
(b) Record the number of crenated red blood cells in the box provided in Table 1.
[3 marks]
(c) Complete Table 2 based on this experiment
Variables

Method to handle the variable

Manipulated variable:
..

Responding variable:

Controlled variable:

TABLE 2
[3 marks]

Biology___________________________________WAJA PPSMI MODULE 2008

(d) State the hypothesis of the experiment.
______________________________________________________________________________
______________________________________________________________________________
[3 marks]
(e) (i) Construct a table and record all data collected in this experiment.
Your table should have the following titles:

The concentration of sodium chloride solution.

Number of each crenated red blood cells in different concentration.

The percentage of crenated red blood cells in different concentration.

Use the formula :

The percentage of crenated red blood cells :
= Number of each crenated red blood cells in different concentration
Total number of crenated red blood cells in different concentration

X 100

(ii) Plot a graph showing the percentage of crenated red blood cells against the
concentrations of sodium chloride solution on a graph paper provided in page 10.
[3 marks]
(f) Referring to the graph in (e)(ii), describe the relationship between the number of
crenated red blood cells and the concentration of sodium chloride solution.
__________________________________________________________________________
__________________________________________________________________________
__________________________________________________________________________
[3 marks]

Biology___________________________________WAJA PPSMI MODULE 2008

(g) In another experiment the red blood cells were immersed in distilled water for 30
minutes. Predict the results of the experiment.
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______________________________________________________________________
[3 marks]
(h) Based on this experiment, what can you deduce about osmosis?
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______________________________________________________________________________
[3 marks]

plasmolysis, crenated, deplasmolysis, turgid

(i) Classify the words given above into Process and Appearance of Cells according to
hypertonic and hypotonic solutions.
Process

Appearance of Cells

[3 marks]

Biology___________________________________WAJA PPSMI MODULE 2008

A graph of the percentage of crenated red blood cells against the concentrations of
sodium chloride solution.