1

1.

:
(denaturation)

2.
salting in 0.5~1M NaCl.(NH4)2SO4

salting out
3.()

4. ()(PI) PH4~
PH
2
1
2 ml sample + 2 ml
10 ml
2
2 ml sample + 10 ml 95
2 ml sample + 5 1NaCl + 10 ml 95 2
3
2 ml sample 0.5 ml
1 ml
3
1. (P.38)
2. (P.38)

1.

2.Protein Structure
http://www.youtube.com/watch?v=lijQ3a8yUYQ&feature=related

3.

4. b
a

(IV)100g .
130
80-13080

()
( 2 )

(AV) 1g ()
KOH .
()
(POV)1000g
().
(SV) 1g
KOH .(++)
TBA()(
)+ (TBA)

()

()TBA

. 1g .
KOH . KOH .
()
(AOM) 2.33ml/
1000g . 20
(meq).

(OH)

65.
.

(
)
()AOM