Discovery Services

Creative Biolabs is a leading service provider that

focuses on developing proteins and high specific, high
affinity monoclonal antibodies for research, diagnostic
and therapeutic use. Our service portfolio includes biofishing platform, epitope mapping, antibody discovery
using phage display technology and a wide range of
antibody gene engineering, affinity maturation and
humanization services.

Phage Display & Antibody Library

Services
Creative Biolabs is professional in applying phage display technology to generate
various phage display peptide, protein, antibody libraries for drug development and
antibody discovery purposes. Our services involve the construction and screening
of custom phage display libraries. In addition, Creative Biolabs is also wellrecognized as a leading provider for a series of phage display based services,
such as specific antibody discovery, humanization, caninization, affinity maturation,
etc.
Phage display is one of the most widely used laboratory technique for the study of
protein-protein, protein-peptide and protein-DNA interactions. This technology is
mainly based on the use of filamentous phage which is a kind of virus lives
on Escherichia coli and has been proven as a powerful method to interrogate
libraries containing millions or even billions of different peptides or proteins.
Theoretically, phage display is an exogenous gene expression method which the
gene encoding the interest protein is inserted into bacteriophage coat protein gene
then displaying the interest protein on the phage surfaces, resulting in a connection
between genotype and phenotype. Through DNA manipulation, numerous gene
variants can be created and constructed as phage display library. To detect the
interaction between displayed protein and those other molecules, phage display

library can be screened to against other proteins, peptides or DNA sequences for
selecting specific binders.
One of the most successful applications of phage display technology has been the
isolation of specific antibodies. As the first proteins which is successfully displayed
on the surface of phage, antibody phage display is achieved by fusing the coding
sequence of scFv or Fab to the coat protein. Due to the smaller size, scFv libraries
are genetically more stable than Fab libraries, while Fab libraries lack the tendency
to form higher molecular weight species, such as dimers and trimers, which can
simplify the selection and characterization. Through binding the specific target, the
interest antibody can then be eluted and retrieved. In this way, Creative Biolabs is
able to obtain various kinds of novel antibodies, such as specific monkey (NHP)
and canine (dog) monoclonal antibodies and anti-idiotype antibodies.
Creative Biolabs has long-term devoted to the development and application of
phage display technology. With years of experience, our scientists have developed
several phage display based platforms and tailored hundreds of particular libraries
and thousands of specific antibody products to boost our global customers
research and project goals. We are pleased to use our extensive experience and
advanced platform to offer the best service and the most qualified products to
satisfy each individual demand from our customers.