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1 Παρουσιαση - ΕΙΣΑΓΩΓΗ ΣΤΗΝ ΧΡΩΜΑΤΟΓΡΑΦΙΑ
1 Παρουσιαση - ΕΙΣΑΓΩΓΗ ΣΤΗΝ ΧΡΩΜΑΤΟΓΡΑΦΙΑ
.
.
(Gas Chromatography, GC)
(Liquid Chromatography, LC)
(CO2 50 15mPa)
(superficial
fluid chromatography, SFC).
:
(GSC)
(LSC).
(GLC)
, (LLC).
(, S, ECD, NPD, FPD
..)
.
1. (adsorption),
().
,
.
2. (partition absorption),
,
.
,
,
(reversed phase chromatography).
3. (ion chromatography),
.
4. (molecular
exclusion chromatography),
,
. ,
.
5. (affinity chromatography),
.
-
,
:
.
,
(
PC TLC)
,
,
, , .
-
,
.
,
,
.
(elution chromatography),
, .
,
.
,
.
,
.
-
K=
CS
CM
( )
.
(
)
.
.
VR
tR
F =
( / )
tR
VR
=
F
RF (retention
ratio)
RF =
L
R
tM
tM
=
tR
V
=
V
M
R
L = , tM = ( )
VM = ( )
-
, RF
,
.
RF =
VS =
CM :
VM
VM
RF =
=
VM + KVS VS
C M VM
=
C M V M + C SV S
V
V
M
R
VR = VM + KVS
VM VS
.
-
,
2,5 ml 4,2 ml.
14,7
8,32 .
VR = VM + KVS
VR,A = 4,2 + (14,7)(2,5) = 40,95 ml
VR,B = 4,2 + (8,32)(2,5) = 25 ml
VR = 40,95 25 = 15,95 ml
-
2 :
,
.
.
,
.
()
.
,
.
().
= h = L / n
n =
-
Gauss
n = 16 (t R , A / wb ) = 16 (V R , A / wb )
2
.
,
tR,A, V R,A, wb
.
.
n
h.
n h
van Deemter
B
h = A +
+ Cu
u
A, B C =
.
-
B
h = A+
+ Cu
u
. .
.
h.
/
(, ).
.
Cu )
)
. ,
,
(
).
-
: u = C
h = + 2 C
, C, ,
- 3
- n :
- h
h = L /n
h h = A +
B
+ Cu
u
-
-
2
.
R :
t R ,B - t R , A
2( t R ,B - t R , A )
t R
R=
=
=
4
0,5(w + w B )
w +wB
R 1,5
-
.
.
k
'
N a 1
B
R=(
)(
)(
)
a 1+ k'
4
B
= / ( = )
= ( = (+)/2).
=
( )
VS
CSVS
'=
=K
CMVM
VM
:
, C
,
.
-
t R = t M (1 + k ' )
tm = (
=0)
t'R = tR tM
V'R =VR VM
rB/A
n = 16(t R , A / wb ) 2 = 16(VR , A / wb ) 2
k' 2
n N
N = n(
)
1+ k'
tR,A= 14,80 tR,= 16,14 min.
L= 40 cm. wA= 1,28 wB= 1,40 cm.
)
) )
)
1,50
:
) h = 0.3mm
h=0.2mm
) n = 20000 n
=10000
) = 0.5 = 0.2
) = 30.000 = 15.000
(GC).
tR (min) w (min)
8,36
0,96
-
9,54
0,64
-.