Isolation, Characterization, and Identification of Lactic Acid Bacteria with

Antifungal Activity from Fermented Traditional Food

Rezcha Indriati Y.
09/284635/BI/8269
ABSTRACT
A lactic acid bacteria (LAB) that has a potential antifungal activity was
isolated from traditional fermented foods. Five grams of each traditional
fermented food samples were homogenized with 45 ml of peptone solution using a
blender. The suspension were inoculated using streak plate method on MRSA
medium supplemented with CaCO3. Suspected isolates as LAB was selected for
antifungal ability using overlay method on MEA soft medium containing mold
spores (Aspergillus flavus FNCC 6002, A. parasiticus FNCC 6033, and
Penicillium citrinum FNCC 6111) and agar diffusion method. Five isolates with
the highest activity were tested using calculation mold biomass testing. An isolate
with the highest activity was characterized and identified using API 50 CHL kit.
Twenty eight LAB isolates were obtained from 5 samples (mocaf, gatot, tiwul,
kara benguk tempeh and soybean tempeh). Then the antifungal activity was
examined further using overlay test and agar diffusion method to produce 27 LAB
isolates that were able to perform fungal growth inhibition. Five isolates with the
highest antifungal activity were G7 isolate from gatot, K1 from soybean tempeh,
M1 and M6 from mocaf, and T9 from tiwul. These isolates were selected to be
tested using calculation mold biomass method. The G7 isolate was able to reduce
63,17% amount of mold biomass, followed by M6 was 62,32%; M10 was 59,13%;
T9 was 51,65%; and K1 was 41,15%. Therefore, G7 isolate was selected to be
characterized and identified further. Based on colonial and cell morphological
characteristics and identification results of API 50 CHL kit, G7 isolate was known
as a member of Lactobacillus plantarum strain with 99,3% similarity. Thus
concluded that LAB which is L. Plantarum G7 that was isolated from gatot has
antifungal activity to inhibit fungal growth.
Keyword : lactic acid bacteria, gatot, antifungal, API 50 CHL kit