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Hand Held Distributor Manual - 2012
Hand Held Distributor Manual - 2012
Refractometers
Distributor Manual
2012
Version 3.3 July 2012
Restricted Publication:
Official Distributors of
Bellingham + Stanley Only
DISTRIBUTOR MANUAL
CONTENTS
1. Introduction
2. Basic Principles
3. Scales and Practical Uses of Refractometers
4. Hand-Held Refractometers
5. The Eclipse Design
6. Commercial Considerations
7. Eclipse Models
8. Eclipse Standard Models
(scale details and application suggestions)
9. E-Line Models
10. E-Line Adblue Models
11. E-Line 80 Model
12. OPTi - Specific Models
Appendix 1
1. Introduction
Bellingham + Stanley has been manufacturing hand-held refractometers since the
1930s when they were first introduced for checking the ripeness of sugar cane in the
field. Nowadays, hand-held refractometers are used all over the world for various
applications. They are now manufactured in very large quantities by quite a number
of companies, many of which are located in the Far East.
This document is a manual designed for Bellingham + Stanley distributors that
market and sell our range of hand-held refractometers. It contains a variety of
information, both technical and commercial, to help the distributor to provide a more
informed service to customers and to aid the selling process.
Our hand held brochure is also informative, but this is targeted for the end-user. The
distributor needs a better understanding of the basic principles and limitations of
hand-held instruments, particularly where customers are contemplating the purchase
of a bench instrument. The latter costs between 20 and 50 times that of a hand-held
refractometer and so the distributor needs a good understanding of the advantages
of the bench instrument and the reasons why hand-held refractometers are limited in
their capability.
This manual therefore provides both promotional materials for our hand held
refractometers, but also criticism of hand-held refractometers generally, so that the
distributor has a better perspective on the industry and is able to satisfy customer
needs more effectively.
Information is also provided on applications to enable the distributor to target
particular subjects. This is not an exhaustive treatise on the subject; further help and
support is always available from the B+S Sales Departments in Tunbridge Wells,
England and Atlanta, USA.
Discussion of hand held refractometers, there strengths and limitations and
particularly in relation to competitor products, necessitates that we must treat this
document as CONFIDENTIAL. Circulation of this document is therefore restricted to
authorised distributors for Bellingham + Stanley.
LEARN MORE..
Visit: www.bellinghamandstanley.com/HHDCRC
2. Basic Principles
To understand the uses and limitations of hand-held refractometers, it is instructive to
consider first the concept of refraction and its measurement.
When a ray of light passes from one medium to another, the speed of the light
changes according to the density of the transmitting medium. At the interface
between two media, the ray changes direction as its speed suddenly changes. This
effect is known as refraction and is a familiar concept.
The refractive index (RI) of a substance is a measure of the speed of light in a
substance relative to that in a vacuum (very close to the speed in air). The RI is a
physical property that depends upon temperature and the wavelength of the light.
For a particular substance the RI is a unique number when measured using a
monochromatic light source (single wavelength) at a fixed temperature.
A refractometer is a device that measures the RI of a substance, usually a liquid,
but sometimes a solid. Laboratory refractometers utilise monochromatic light, usually
that of sodium at 589.3 nm. They also have a means for controlling temperature or at
least measuring it precisely in order to compensate for any variance. A laboratory
refractometer can typically measure the refractive index to within 0.0001 or better.
Thus, the refractive index of water when measured with sodium light (589.3 nm) at 20
C is 1.33299.
How does a refractometer work? Most modern refractometer designs utilise the
concept of Critical Angle, which is attributed to Ernst Abbe, the 19th Century
physicist. The principle is illustrated below.
Incident light strikes the surface
of a medium at different angles.
At low angles the light passes
through the substance and this is
called refracted light. At a certain
angle of incidence called the
Critical Angle, the light no longer
passes through; it is reflected
back. From the viewing position,
an area of light and an area of
dark are therefore observed,
corresponding to transmitted
(refracted) light and reflected light
(no transmission), respectively.
The Borderline between the two
areas defines the critical angle
condition and this can be
mathematically related to the RI
of the substance.
Liquid Sample
Prism
80
60
40
20
0
This is the basic concept, but to build a refractometer, other design features must
then be considered:
1. Prism this supports the sample and guides the light rays through the
refractometer. It must be made from a substance of RI higher than that of the
measured substance usually a special glass or sapphire. Prism angles
determine the direction of emergent light rays
2. Scale this is mathematically constructed according to the geometry of the
instrument and the RI of the prism. Its intersection with the observed borderline
(critical condition) provides the reading.
3. Focusing system lenses are used to enable the user to focus simultaneously
on the borderline and the scale.
4. Light source this is ideally a pure (monochromatic) source, but natural (white)
light can be used, for example with hand-held refractometers.
5. Temperature control this is a means whereby the sample temperature is
controlled or precisely measured.
These features govern the main aspects of a basic, optical-mechanical refractometer.
Modern digital automatic instruments utilise the same concept and principle, but
electronics and software are used to provide greater user flexibility, convenience and
performance features.
Scanning through the Eclipse range in the later parts of this manual will illustrate
quite a number of the popular standard scales that are available. Some are very
specific such as those used in the wine and brewing industries and the antifreeze
scales used for automotive fluids and the clinical scales used in hospitals for urine
and blood plasma. We have even used a scale for measuring the ripeness of grass
(Water soluble Carbohydrate content) for use by farmers.
Basically, provided a relationship between liquid composition or concentration and RI
is available, a special scale can be constructed for a refractometer making it a
concentration meter.
4. Hand-held refractometers
So, what about hand-held refractometers? They are low-cost, simple devices that are
popular in a multitude of applications. They are popular because they are easy and
convenient to use and cost a fraction of a typical bench instrument.
Hand-held refractometers work according to the same basic principles and design
considerations outlined above. But they are limited in terms of accuracy and
applicability because:
1. They utilise natural (white) light
2. There is no way to control temperature
3. Light must be transmitted by the sample
Using white light means that the borderline cannot be as sharp as that obtained in a
laboratory instrument. White light is made up of wavelengths from about 350 to 800
nm (the visible spectrum). Light of each wavelength travels at a different speed. This
manifests itself as blurring and colouring of the borderline. This is called dispersion
(splitting into different wavelengths). Some substances have high dispersive power
and therefore will distort a white light borderline to a greater extent. This effect gets
worse as the RI increases, such that some liquids may not be measurable to an
acceptable accuracy using a hand-held instrument.
The accuracy and precision is also limited by the size and optical arrangement.
Typically, a hand-held refractometer can read on an RI scale to about 0.001 units,
compared to a resolution of up to 0.00001 on a bench Abbe or automatic instrument.
These values equate to about 0.2 Brix for a hand held versus a resolution to 0.01
Brix on a bench instrument such as the RFM 340+. Accuracy on the latter will vary
according to sample type but can be about 0.02 for a sugar solution or soft drink,
i.e. about 100 times more accurate than a hand-held refractometer.
Despite the fact that some hand-held models offer Automatic Temperature
Compensation (ATC), there is no actual control over the sample temperature. The
ATC principle (explained below in section) gives a limited degree of temperature
correction, relying upon the sample being at the same temperature as the instrument.
Most hand-held refractometers are calibrated initially at 20 C in the factory. When
the ambient temperature changes, the instrument temperature also changes and the
calibration is no longer valid. Certain hand held refractometers such as the Eclipse
have a zero adjuster. This means the instrument can be re-set using a reliable
standard such as water. This is in fact a better way to combat changes in
temperature with a hand-held instrument than relying upon ATC. Sudden changes in
temperature or applying very hot or very cold samples (e.g. out of the refrigerator)
can incur quite large errors with hand-held instruments. The way to deal with this is to
have good experimental technique/protocol to ensure thermal equilibration.
Because hand-held refractometers work with transmitted light, highly coloured or
opaque samples may not give very sharp borderlines. The position of the borderline
then becomes a subjective judgement of the user, which reduces the reproducibility
of the instrument. Bench automatic instruments, such as the RFM models, surmount
this problem by measuring the reflected light from a sample surface, rather than
transmitted light. Thus, for example in the tomato paste industry, bench automatics
are considerably easier to use and much more reliable than hand-held refractometers
where borderlines are often not discernible.
In practice, many users are happy with the relative low accuracy of hand-held
refractometers, accepting that they provide a quick and convenient way to check their
product, rather than a high precision QA method. Other users really do not
appreciate the fact that a hand-held refractometer is such a basic device compared
to a laboratory bench instrument. Distributors for B+S need to understand this and
give fair and realistic advice depending on the customer specific application.
6. Commercial Considerations
B+S would like to achieve a leading position alongside Atago in this high volume
hand-held refractometer market. B+S have a strong position in the bench
refractometer market with the RFM ranges and many potential customers are aware
of our market presence. We are the leader in many world markets with digital
automatic bench instruments. However, our higher prices have precluded us from
many hand-held markets; many potential customers may assume that we are too
expensive. We want to change this perception.
We believe the Eclipse is a high quality instrument and we believe we can offer the
instrument at a competitive price in most/all markets. However, we need close cooperation and aggressive marketing from our distributors to achieve our sales
aspirations. As our sales grow we aim to improve our manufacturing methods and
any cost savings will be reflected in our pricing
We have priced the instrument in a different way to other products. The Eclipse is 20
to 50 times lower in price than our laboratory instruments. Its cost (price) is therefore
much more sensitive to costs incurred in packing, freight and administration.
Therefore, to achieve our goals of widespread sales and rapid sales growth, we must
try to sell and ship in quantity. We therefore offer a steep volume-discount
relationship to encourage volume purchase. We wish our distributors to purchase
higher quantities for stock: 10+, 25+, 50+ etc. We realise that some judgement is
needed in selecting the right models to suit the local market and therefore we can
assist with this process and some negotiation may be necessary.
7. Eclipse Models
Manufactured in the UK using only the highest quality optical components and the
most modern manufacturing practices, the Eclipse refractometer is the ultimate hand
held refractometer on the market today. A comprehensive choice of scale types
offers versatility across a wide application scope from testing fruit ripeness in the field
to monitoring industrial fluids in harsh machine shop environments. Eclipse
refractometers have a number of unique features, which are not available on many
other brands of refractometers.
Code
45-01
45-02
45-07
45-03
45-08
45-05
45-06
45-22
45-27
45-81
45-82
Code
45-26
45-41
45-44
45-45
45-46
45-65
45-66
Range
Scale Division
0 15
0 30
0 32
0 50
28 65
45 80
72 95
10 135
10 30
0 50
45 80
Range
0 30
1.330 1.420
0.1
0.2
0.2
0.5
0.2
0.2
0.2
1.0
0.2
0.5
0.2
Scale Division
0.2
0.001
0 to 40
0 to 40
1.1 1.35
5.0
5.0
0.05
30 to -40
30 to -40
1.1 1.35
5.0
5.0
0.05
0 60
0 60
0 28
1.330 1.420
2.5
2.5
0.2
0.001
Page
No
10
11
12
11
11
11
12
13
13
18
18
15
16
17
17
18
45-003
44-838
44-839
18
16
Code
Range
Sugar % (Brix)
Sugar % (Brix)
Sugar % (Brix)
Sugar % (Brix)
Sugar % (Brix)
Sugar % (Brix)
Sugar % (Brix)
45-01
45-02
45-07
45-03
45-08
45-05
45-06
0-15
0-30
0-32
0-50
28-65
45-80
72-95
Scale
Division
0.1
0.2
0.2
0.5
0.2
0.2
0.5
Weight
g
280
250
250
220
250
250
220
Length
(mm)
230
190
190
160
190
190
160
B+S are often asked why we do not produce a single 0-95% instrument. The reason
is because an optical design based on acceptable dimensions would compress the
scale and reduce reading resolution or scale magnification would make viewing of
the scale awkward.
The latter argument applies to very short-range instruments, for example 0-10%. To
make such an instrument with an acceptable comfortable to read, non-obscured
scale, would require a long body. We prefer to stop at the 0-15% design which still
maintains comfortable viewing. Some competitors offer 0-10% models, but we do not
like the optical design.
Some of our competitors offer a 0-95% instrument with three interchangeable scales.
We do not wish to make such an instrument for a number of reasons:
- there are not many applications that require the full scale
- it is a relatively expensive instrument
- it is mechanically more complex and therefore subject to faults
- two standard models are probably a better and cheaper option
Model 45-01 (0-15 Brix)
AVAILABLE
10
AVAILABLE
AVAILABLE
AVAILABLE
11
AVAILABLE
Temperature-Compensated Eclipse
Model 45-91 (0-30 Brix, ATC)
AVAILABLE
12
Water in Honey
Model 45-27 (10-30 % Water in Honey)
The application is specific to controlling the quality of fresh honeys. Many instruments
are sold to bee-keeping associations or specialist suppliers. As with the 45-06 model,
re-calibration may be a problem because a standard within the RI 1.48 to 1.51 range
is needed: oil or possibly a test plate (see Appendix 4). For percent water in honey
temperature correction table (see Appendix 7).
AVAILABLE
13
ABV Kit
Consisting of:
Measurement method:
1. Siphon off enough of the finished sample to fill the hydrometer jar to
the required level and leave all the equipment with it in a place free
from draughts and direct sunlight for a least an hour to attain room
temperature.
2. Measure the S.G. as accurately as possible and record
3. Measure the refractive index of the sample in Zeiss units using the
Eclipse Wine refractometer.
4. The alcohol content in %ABV can be read from a graph or
alternatively, calculated from the R-D value as shown below to obtain
%ABV from a table supplied with the instrument.
R-D = R (Refractometer reading) D (S.G. value)
Where D (S.G. value) = (S.G. 1) x 1000
AVAILABLE
Examples
Light dry table wine
S.G. = 0.993 & Refractometer reading = 37
D (S.G. value) = (0.993 1) x 1000 = -7
R-D = 37 (-7) = 44
Alcohol content = 10.7%v/v
Sweet dessert wine or Sweet Cider
S.G. = 1.015 & Refractometer reading = 72.5
D (S.G. value) = (1.015 1) x 1000 = +15
R-D = 72.5 (+15) = 57.5
Alcohol content = 15.7%v/v
14
The following table shows the relationship between the various wine scales and the
corresponding RI.
RI
1.33299
1.33442
1.33586
1.33732
1.33879
1.34026
1.34175
1.34325
1.34477
1.34629
1.34782
1.34937
1.35093
1.35250
1.35408
1.35568
1.35729
1.35891
1.36054
1.36219
1.36384
1.36551
Sugar %
(Brix)
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
16.0
17.0
18.0
19.0
20.0
21.0
Zeiss
14.5
18.2
21.9
25.7
29.6
33.4
37.4
41.3
45.4
49.4
53.5
57.7
62.0
66.2
70.6
75.0
79.4
83.9
88.5
93.1
97.7
102.5
Oechsle Oechsle
(Swiss) (German)
0.1
3.2
3.9
7.0
7.8
10.8
11.8
14.6
15.7
18.5
19.7
22.4
23.7
26.3
27.7
30.3
31.8
34.3
35.9
38.3
40.0
42.4
44.2
46.4
48.4
50.6
52.6
54.7
56.8
58.9
61.1
63.1
65.4
67.4
69.8
71.6
74.1
75.9
78.5
80.3
83.0
84.6
87.4
89.0
Baum
0.0
0.6
1.1
1.7
2.2
2.8
3.3
3.9
4.5
5.0
5.6
6.1
6.7
7.2
7.8
8.3
8.9
9.4
10.0
10.5
11.1
11.6
% Probable
Alcohol
0.0
0.6
1.2
1.8
2.4
3.0
3.7
4.3
4.9
5.5
6.2
6.8
7.4
8.1
8.7
9.4
10.1
10.7
11.4
12.1
AVAILABLE
Starch
Model 45-26 (0-30 %)
This instrument can be used to test starch solutions
in the food industry but also a particular application is
to test starch solutions used for coatings in the paper
industry.
The instrument can be zeroed with distilled water.
15
AVAILABLE
Refractive Index
Model 45-41(1.33 1.42 / resolution 0.001)
This is the fundamental scale from which all other
scales derive. It can, in principle be used for any
application, provided the user knows the relationship
between RI and the parameter in which they are
interested. The scale is used for scientific purposes
and where there is no other convenient scale
available.
N (sometimes n) is the scientific symbol for refractive index and nD denotes RI at the
sodium D line (589.3 nm).
The instrument can be used for a wide range of chemicals and their solutions.
Examples are detergents, flavours and essences. Some customers in the car and
aircraft industries use this model for measuring anti-freeze fluids.
The instrument can be calibrated with water. At 20 C, the RI of water = 1.33299. To
set the instrument at other temperatures, the table in Appendix 1 can be used.
Note B+S used to offer a higher range RI instrument (1.40-1.50), but demand for this was
very small. We do not offer such an instrument in the Eclipse range because of problems with
dispersion. However, as an alternative the 44-829 E-line model which has a range of 1.401.50 R.I. can be considered or the E-line 90 model which also features achromatising prisms,
making it ideal for use with samples that do not have the same dispersion characteristics as
sugars.
Refractive Index
Model 45-66 (1.33 1.42 Aviation / resolution 0.002)
This instrument has been specifically
designed for the aviation industry. The
Eclipse Aviation is used for determining the
concentration of aircraft anti-icing fluids
(glycol & additives) when mixed with water
prior to its application to aircraft wings in
cold weather to prevent ice build up during
take-off. It incorporates a clearly defined
scale for the easy reading of refractive
index, which is then converted using a table
supplied by the fluid manufacturer to
acertain the precise concentration and/or
the freezing point of the mixture. The
Eclipse Aviation has a secure protective
zero cap to ensure that no accidental
adjustments are made to the calibration,
thus ensuring accurate and reliable results
are maintained. However, the calibration
of the refractometer should be periodically
verified using distilled water at 20C.
AVAILABLE
16
Antifreeze/Automotive
Model 45-44 triple scale instrument:
- C protection (ethylene glycol)
- C protection (propylene glycol)
- Battery acid SG
AVAILABLE
AVAILABLE
17
Model 45-46
Ethylene glycol, propylene glycol 0-60 % vol
AVAILABLE
Salinity
Sodium Chloride
Model 45-65 (0-28 % weight)
This instrument is used in a number of applications. In the food industry it can be
used to test brines used for canning/packing vegetables and for meat and fish
preservation. It can be used to test brackish waters in de-salination operations. It can
also be used to test the salinity of waters used in aquariums and for fish farming
purposes. It may also be used by some petroleum engineers in the preparation of
brines used for oil well drilling fluids and checking the salinity of production fluids
from reservoirs.
AVAILABLE
In principle, other scales can be created for particular inorganic salts, provided data
on RI vs. concentration are available
18
Focussing
the scale
Taking a
reading
Cleaning the
prism
Lift the flap, drip sample onto the prism then lower the flap or use the
dribble feature; drip sample onto the top of the closed flap. The
Eclipse can also be used for testing solid substances (fruits). Cut a
slice of the substance about 2mm thick and slightly smaller than the
prism area. With the flap lifted, apply the slice to the surface of the
prism.
Hold the instrument up to the light and look through the eyepiece.
Rotate the eyepiece to focus the scale.
Read the scale at the border of the light and dark areas. If the scale
is completely light then the sample concentration may be too high for
the instrument range.
Thoroughly clean the prism and flap immediately after use with water
or other suitable solvent and dry with clean tissue. Wiping the prism
surface occasionally with alcohol will remove any build-up of oils left
from the samples.
19
9. E-Line Models
The E-line Automatic Temperature Compensation (ATC) Range of hand held
refractometers offers versatility at a low price. They are ideal for use in many applications
including: fruit, beverage, sugar and industrial applications where concentration
measurements are required.
Code
44-801
44-802
44-803
44-804
44-805
44-812
44-823
Water in Honey
Wort Specific Gravity (SG)
Sugar % (Brix) ATC
Special Application Models
44-808
Saline o/oo (PPT)
Saline SG
44-820
EG/PG C Protection
EG/PG % vol/vol
44-821
EG/PG C Protection
Battery SG
Windscreen C
44-825
Serum Protein g/100ml
Refractive Index
Urine SG
44-828
Refractive Index ATC
44-829
Refractive Index ATC
44-822
Automotive Adblue
EG/PG C Protection
Wine Applications
44-809
% Mass (Brix)
Alcohol Probable
44-817
% Mass (Brix)
Oechsle D
44-818
% Mass (Brix)
Oechsle
Babo (KMW)
44-819
Baum
Alcohol Probable
Range
Scale Division
Page
No.
21
21
21
21
21
0 10
0 18
0 32
28 62
45 82
0.1
0.1
0.2
0.2
0.5
10 30
1.000 1.120
0-32
0.1
0.0005
0.2
22
23
0 100
1.000 1.070
0 to -50C
0 to 70%
0 to -50C
1.10 to 1.40
0 to -40C
0 12
1.335 1.360
1.000 1.050
1.30 1.38
1.435 1.520
30 35 %
0 to -50C
1.0
0.001
5C
5.0
5C
0.01
5.0C
0.2
0.0005
0.005
0.0001
0.001
0.5
5.0
22
0-40
0-25
0 32
0 140 OeD
0 32
0 140 Oe
0 27
0 20
0 25
0.2
0.2
0.2
1.0
0.2
1.0
0.2
0.2
0.2
23
23
24
24
24
25
22
22
22
23
20
AVAILABLE
AVAILABLE
AVAILABLE
AVAILABLE
Note
Special calibration fluids (accurate sugar solutions)
or solid test plates are needed to verify the calibration
because water is off-scale.
AVAILABLE
21
AVAILABLE
AVAILABLE
AVAILABLE
AVAILABLE
22
AVAILABLE
AVAILABLE
AVAILABLE
AVAILABLE
23
AVAILABLE
AVAILABLE
AVAILABLE
24
AVAILABLE
AVAILABLE
25
44-881
Lift the flap, drip sample onto the prism then lower the flap. The ELine can also be used for testing solid substances (fruits). Cut a slice
of the substance about 2mm thick and slightly smaller than the prism
area. With the flap lifted, apply the slice to the surface of the prism.
Hold the instrument up to the light and look through the eyepiece.
Rotate the eyepiece to focus the scale.
Read the scale at the border of the light and dark areas. If the scale
is completely light then the sample concentration may be too high for
the instrument range.
Thoroughly clean the prism and flap immediately after use with water
or other suitable solvent and dry with clean tissue. Wiping the prism
surface occasionally with alcohol will remove any build-up of oils left
from the samples.
Spare illuminator flaps with collar for ATC E-Line models (pack of 5)
Select
correct range
Applying the
sample to the
refractometer
Focussing
the scale
Taking a
reading
Cleaning the
prism
AVAILABLE
Range
Scale Division
0 80
1.0
27
Measurement Performance
Automatic Temperature
Compensation (ATC)
540C
5-90C
1C (540C)
Measurement time
2 seconds
Relative humidity
Yes
Sample indicator
IP rating
Battery type
3V 2 x AAA (LR03)
Physical Performance
Prism material
Optical glass
Prism seal
Sample dish
8mm
Sample volume
0.3ml
Case material
28
User manuals1(English, French, German, Spanish, Italian & BrazilianPortuguese), Certificate of Calibration & soft black protective case
Warranty (limited)
12-months
Shipping Information
Dimensions (unpacked)
Dimensions (packed)
Weight (unpacked)
0.1kg
0.2kg
2.5kg
Commodity code
90275000
Service
OPTi Service
OPTi Custom
OPTi Scale Creation
Instrument overview
Prism dish
Prism surface
LCD display
Battery
Compartment cover
Model/scale
Identification
label
ZERO key
READ key
Battery indicator
Scale identifiers
OPTi Duo models only
29
Basic Operation
Installing batteries
Remove the battery compartment cover by turning the two retaining screws in an
anti-clockwise direction. Before inserting the batteries check that the compartment is
clean and dry, and hat he cover seal is in good order. Insert the batteries, ensuring
that the battery polarity is correct. Replace the cover by turning the two retaining
screws clockwise direction whilst the cover is in position.
It is recommended that alkaline batteries are used to reduce the frequency of the
battery changes
The battery indicator will show the current state of the batteries. When the indicator
shows empty replace the batteries never leave exhausted batteries in the
refractometer.
Turning on and off
To turn the instrument on press READ. The instrument will automatically turn off if no
buttons have been pressed for 60 seconds alternatively press and hold READ for 3
seconds to switch the instrument off.
Taking a reading
Before taking a reading clean the prism surface thoroughly using a suitable solvent,
e.g. water or methyl alcohol depending on the sample being measured.
Fill the prism dish with the sample.
Press the READ key, the display will clear
A few seconds later the reading will be displayed. OPTi Duo models will also indicate
the selected measurement scale on the display. After a measurement has been
taken the sample should be removed and the prism cleaned.
Zero calibration
Fill the prism dish with distilled water. If the water is not at ambient temperature allow
time for the water to temperate.
Press and hold ZERO for 3 seconds. The display will show
starts. When complete the display will show
as the calibration
30
31
Error messages
In order to achieve the maximum performance from the OPTi refractometers, it is
essential that care is taken when cleaning the instrument and applying samples to
the prism. Sample concentration may vary considerably from the surface to the
centre of a mass whether in a beaker or on a spoon or spatula. Evaporation will
cause the reading to drift unless care is taken
Measured sample out of range.
Sample either too low or high or of insufficient volume
Temperature too low or high.
Battery too low
Excessive ambient light or insufficient sample
(flashes)
--.-C/F
No recorded temperature
-AGt- / 40.1
(alternates)
Declaration of Conformity
(According to ISO/IEC 17050-1 & 2 : 2004)
Manufacturers Name:
Manufacturers Address:
Longfield Road
Tunbridge Wells
Kent TN2 3EY
United Kingdom
Model Number:
All
Emissions
EN 61326-1:2006
AS/NZS CISPR 11
FCC/CFR 47: part 15
Canadian Standard ICES-003:Issue 4
Immunity
EN 61326-1:2006
EN 61326-1:2006
Supplementary
32
33
Type
Scale
Range
Resolution
Accuracy
38-02
Brix 54
Sugar % (Brix)
38-17
RI 1.42 NTC
38-19
ATC
I.D.
0-54
0.1
0.2
Bx
S54t
1.33-1.42
0.1
0.2
NONE
Lndn
Brix 54 NTC
Sugar % (Brix)
0-54
0.1
0.2
NONE
S54n
38-20
RI 1.42
1.33-1.42
0.0001
0.0003
Bx
Lndt
38-22
Starch 30
Starch %
0-30
0.1
0.2
Bx
St30
38-23
Urea 40
0-40
0.1
0.2
AUS32
UrEA
38-25
Seawater SG
1.000-1.090
0.0005
0.001
NaCl
SES9
38-26
Seawater PPT
0-180
NaCl
SEPt
38-27
Wort SG
1.000-1.120
0.0005
0.001
Bx
bEEr
38-28
Saline 28
Salinity % (NaCl)
0-28
0.1
0.2
NaCl
NACL
38-29
Adblue
Urea % (AUS-32)
0-40
0.1
0.2
AUS32
AdbL
Type
Scale
Range
Resolution
Accuracy
38-04
Jam 75
Sugar % (Brix)
38-05
Hi Brix 95
38-06
ATC
20-75
0.1
0.2
Bx
J75t
Sugar % (Brix)
50-95
0.1
0.2
Bx
H95t
Honey
Water in Honey %
10-30
0.1
0.2
Honey
Hy30
38-07
Hi HFCS 42
42 HFCS
50-95
0.1
0.2
Bx
HF1
38-08
Hi HFCS 55
55 HFCS
50-95
0.1
0.2
Bx
HF2
38-09
Hi HFCS 90
90 HFCS
50-95
0.1
0.2
Bx
HF3
38-16
Hi RI 1.54 NTC
1.42-1.54
0.0001
0.0003
NONE
Hndn
38-18
Hi Brix 95 NTC
Sugar % (Brix)
50-95
0.1
0.2
NONE
H95n
38-32
Hi RI 1.54
1.42-1.54
0.0001
0.0003
Bx
Hndt
34
I.D.
Type
Scale
Range
Resolution
Accuracy
38-A1
Brix 95+
Sugar % (Brix)
38-A2
RI 1.54+
38-H1
ATC
I.D.
0-95
0.1
0.2
Bx
S95t
1.33-1.54
0.0001
0.0003
Bx
Fndt
HFCS 42+
42 HFCS
0-95
0.1
0.2
Bx
HF4
38-H2
HFCS 55+
55 HFCS
0-95
0.1
0.2
Bx
HF5
38-H3
HFCS 90+
90 HFCS
0-95
0.1
0.2
Bx
HF6
38-B8
Sugar % (Brix)
0-95
0.1
0.2
NONE
S95n
38-B9
RI 1.54+ NTC
1.33-1.54
0.0001
0.0003
NONE
Fndn
Type
38-41
Wine AP
38-42
Wine OE-D
38-43
Wine OE-CH
38-44
Wine KMW
38-45
Wine Baume
38-46
Wine Baume/AP
38-47
ABV
A
B
A
B
A
B
A
B
A
B
A
B
A
B
Scale
% Mass w/w
Alcohol Probable (AP)
% Mass w/w
Oechsle (German)
% Mass w/w
Oechsle (Swiss)
% Mass w/w
KMW (Babo)
% Mass w/w
Baum
Baum
Alcohol Probable (AP)
% Mass w/w
ABV (Zeiss)
Range
0-35
0-22
0-35
30-130
0-35
0-130
0-35
0-25
0-35
0-28
0-28
0-22
0-35
10-135
Resolution
0.1
0.1
0.1
1
0.1
1
0.1
1
0.1
0.1
0.1
0.1
0.1
0.1
Accuracy
0.2
0.2
0.2
1
0.2
1
0.2
1
0.2
0.2
0.2
0.2
0.2
0.5
ATC
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Range
10-135
1.000-1.120
10-135
0-30
0-54
0-60
Resolution
0.1
0.0005
0.1
0.1
0.1
0.1
Accuracy
0.5
0.001
0.5
0.2
0.2
0.4
ATC
Bx
Bx
Bx
Bx
Bx
PG
I.D.
AP
OEd
OECH
bAbo
bAUE
bAAP
APU
Brewing
Code
Type
38-48
Brew SG
38-49
Brew
38-75
Dispense
A
B
A
B
A
B
Scale
ABV (Zeiss)
Wort Specific Gravity (Sucrose Equivalent)
ABV (Zeiss)
Plato
Sugar % (Brix)
Propylene Glycol % by volume
35
I.D.
brE1
brE2
brE3
Life Science
Code
Type
38-51
Aquatic
38-52
Sport
38-53
Vet
38-54
Bx/Saline
38-56
Colostral
A
B
A
B
A
B
A
B
A
B
Scale
Seawater PPT (Parts Per Thousand)
Seawater Specific Gravity (SG)
Urine Specific Gravity (SG) Human (not IVD)
Sugar % (Brix)
Urine Specific Gravity (SG) Small Mammal
Urine Specific Gravity (SG) Large Mammal
Sugar % (Brix)
Salinity % (NaCl)
Sugar % (Brix)
Colostral Quality
Range
0-180
1.000-1.070
1.000-1.050
0-30
1.000-1.050
1.000-1.050
0-54
0-28
0-35
Poor / -PASS
Resolution
1
0.0005
0.0005
0.1
0.0005
0.0001
0.1
0.1
0.1
Accuracy
1
0.0010
0.0010
0.2
0.0010
0.0010
0.2
0.2
0.2
PASS / Good
ATC
NaCl
NaCl
Bx
Bx
Bx
Bx
Bx
NaCl
Bx
Bx
I.D.
LSc1
LSc2
LSc3
LSc4
LSc5
General Purpose
Code
Type
38-31
General Purpose C1
38-37
Chemical C2
38-38
Chemical C3
A
B
A
B
A
B
Scale
Sugar % (Brix)
Refractive Index (RI) ATC
Refractive Index (RI)
Temp C (no ATC)
Sugar % (Brix)
Refractive Index (RI)
Range
0-54
1.33-1.42
1.33-1.42
5-40C
20-70
1.36-1.47
Resolution
0.1
0.0001
0.0001
0.1
0.1
0.0001
Accuracy
0.2
0.0003
0.0003
1
0.2
0.0003
ATC
Bx
Bx
NONE
N/A
Bx
Bx
Range
0-60
0 to -50
0-60
0 to -50
0-60
0-60
30 to -40
0-60
0-60
30 to -40
0-18
1.33-1.38
Resolution
0.1
1
0.1
1
0.1
0.1
1
0.1
0.1
1
0.1
0.0001
Accuracy
0.4
1
0.4
1
0.4
0.4
1
0.4
0.4
1
0.2
0.0003
ATC
PG
PG
EG
EG
EG
PG
PG
PG
EG
EG
Bx
Bx
I.D.
9PC1
9PC2
9PC3
Type
38-70
38-71
38-72
38-73
38-74
38-81
Coolant
A
B
A
B
A
B
A
B
A
B
A
B
Scale
Propylene Glycol % by volume
Propylene Glycol C Protection
Ethylene Glycol % by volume
Ethylene Glycol C Protection
Ethylene Glycol % by volume
Propylene Glycol % by volume
Propylene Glycol F Protection
Propylene Glycol % by volume
Ethylene Glycol % by volume
Ethylene Glycol F Protection
Sugar % (Brix)
Refractive Index (RI)
36
I.D.
HtL1
HtL2
HtL3
HtL4
HtL5
HtL6
Automotive
Code
Type
38-61
Auto A1
38-62
Auto A2
38-63
Auto A3
38-65
Auto A4 DEF/C-EG
38-66
Auto A5 DEF/F-EG
38-67
Auto Antifreeze C
38-68
Auto Antifreeze F
A
B
A
B
A
B
A
B
A
B
A
B
A
B
Scale
Sugar % (Brix)
Ethylene Glycol C Protection
Sugar % (Brix)
DEF Adblue
Sugar % (Brix)
Propylene Glycol C Protection
DEF Adblue
Ethylene Glycol C Protection
DEF Adblue
Ethylene Glycol F Protection
Ethylene Glycol C Protection
Propylene Glycol C Protection
Ethylene Glycol F Protection
Propylene Glycol F Protection
Range
0-54
0 to -50
0-54
0-40
0-54
0 to -50
0-40
0 to -50
0-40
30 to -40
0 to -50
0 to -50
30 to -40
30 to -40
Resolution
0.1
1
0.1
0.1
0.1
1
0.1
1
0.1
1
1
1
1
1
Accuracy
0.2
1
0.2
0.2
0.2
1
0.2
1
0.2
1
1
1
1
1
ATC
Bx
EG
AUS32
AUS32
Bx
PG
AUS32
EG
AUS32
EG
EG
PG
EG
PG
I.D.
AuA1
AuA2
AuA3
AuA4
AuA5
AuA6
AuA7
Heat Transfer Liquids and Automotive ideal for accurately checking the concentration of
certain car fluids
Aviation
Code
38-83
Type
Aviation
A
B
Scale
Refractive Index (RI)
FSII DiEGME ATC
Range
1.33-1.42
0.0 to 0.25
Resolution
0.0001
0.01
Accuracy
0.0003
0.02
ATC
NONE
Bx
I.D.
FS11
Type
38-10
Honey/Brix
38-11
Hi HFCS 42/55
38-12
Hi HFCS 55/90
A
B
A
B
A
B
Scale
Water in Honey %
Sugar % (Brix)
42 HFCS
55 HFCS
55 HFCS
90 HFCS
Range
10-30
50-95
50-95
50-95
50-95
50-95
Resolution
0.1
0.1
0.1
0.1
0.1
0.1
Accuracy
0.2
0.2
0.2
0.2
0.2
0.2
ATC
Honey
Bx
Bx
Bx
Bx
Bx
37
I.D.
HySu
HF7
HF8
Type
38-A3
Brix 95/Honey+
38-B1
38-H4
HFCS 42/55+
38-H5
HFCS 55/90+
38-H6
38-H7
38-H8
A
B
A
B
A
B
A
B
A
B
A
B
A
B
Scale
Sugar % (Brix)
Water in Honey %
Sugar % (Brix)
Refractive Index (RI)
42 HFCS
55 HFCS
55 HFCS
90 HFCS
Sugar % (Brix)
42 HFCS
Sugar % (Brix)
55 HFCS
Sugar % (Brix)
90 HFCS
Range
0-95
10-30
0-95
1.33-1.54
0-95
0-95
0-95
0-95
0-95
0-95
0-95
0-95
0-95
0-95
Resolution
0.1
0.1
0.1
0.0001
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Accuracy
0.2
0.2
0.2
0.0003
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
0.2
ATC
Bx
Honey
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Bx
Accessories
Code
Description
38-016
38
I.D.
FSHy
FSnd
HF9
HF10
HF11
HF12
HF13
Appendix 1
Refractive Index of Water in air and at 589.3nm over the range 5-75C
Temperature tC
5.00
10.00
15.00
20.00
25.00
30.00
35.00
40.00
45.00
50.00
55.00
60.00
65.00
70.00
75.00
R.I. of Water at
589.3nm & tC
1.33388
1.33369
1.33338
1.33299
1.33250
1.33194
1.33131
1.33061
1.32985
1.32903
1.32817
1.32725
1.32628
1.32527
1.32421
Data Source:
Refractive index values for Water are obtained from "Revised Formulation for the Refractive Index
of Water and Steam as a Function of Wavelength, Temperature and Density", adopted by the
International Association for the Properties of Water and Steam (IAPWS) and available as part of
NIST Standard Reference Database 101. Refractive indices calculated from the formulation are
absolute refractive indices; conversion to refractive index against air requires division by the
respective absolute refractive index of air (NIST Engineering Metrology Toolbox2).
Refractive indices of air against temperature are determined under the following conditions:
Vacuum Wavelength: 589.3nm
Atmospheric Pressure: 101.325 kPa
Air Humidity: 50 %RH
Abbe Scale Conversions performed using the Abbe Utility Program V4.0 (10-660)
1
2
NIST Standard Reference Database 10, Version 2.21, A.H.Harvey, A.P.Peskin, S.A.Klein
NIST Engineering Metrology Toolbox: Refractive Index of Air Calculator (http://emtoolbox.nist.gov/Wavelength/Abstract.asp)
Appendix 2
45-02
45-07
45-03
S
U
I
T
A
B
L
E
45-06
45-22
45-27
45-81
45-82
45-91
45-26
45-41
45-45
45-46
45-65
H
A
N
D
45-63
45-92
45-93
N/A
10-40
44-801
44-802
44-804
44-805
44-808
44-809
44-812
44-817
44-818
44-819
44-820
44-821
44-825
44-828
44-829
OPTi
H
E
L
D
R
E
F
R
A
C
T
O
M
E
T
E
R
S
45-94
E-Line
44-807
7-15
F
O
R
45-44
44-803
0-85
Colostral
(mares milk)
N
O
T
7-12
Clinical / VET
0-90
Wine grape
must
5-40
Nectars
40-75
Lemonade
0.2
Sucrose
solutions
5-30
Canned fruits
5-25
Fruits
concentrates
11.2
45-08
45-05
Diet drinks
Orange Juice
50-70
Lactic acid
beverage
45-01
513
General
Juices
5-30
Soft Drinks
Syrups
Typical
Brix
Eclipse
Colas / sodas
Model
No.
General Milk
Products
Single
38-02
38-02
38-05
38-02
38-02
38-02
38-04
38-02
P. 33
38-02
N/A
P. 33
N/A
Duo
38-31
38-31
38-38
38-31
38-31
38-31
38-38
38-31
P. 35
38-31
N/A
P. 34
P. 35
N/A
+Single
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
N/A
38-A1
N/A
N/A
+Duo
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
N/A
38-B1
N/A
N/A
38-56
The instrument selection guide is for general reference only and may not cover all applications or
circumstances. If unsure about an application, please contact our sales department for further
technical advice and assistance.
40
Appendix 2 (continued)
Tomato
ketchup
Syrups/HFCS
Fondants &
chocolate
Toffee
Honey
(moisture)
Condensed
milk
Brines for
canning
Yeast culture
40-75
Edible
starches
5-20
Jams &
marmalade
30-50
5-45
Tomato puree
& pastes
5-20
Apples,
strawberries
Typical
Brix
Eclipse
Analysis of
whole egg
Model
No.
Oranges &
pears
5-20
55-75
5-25
15-35
55-75
55-95
55-95
75-95
40-75
5-20
45-01
45-02
45-07
45-03
45-08
45-05
45-06
45-22
45-27
45-81
45-82
45-91
45-26
45-41
45-44
45-45
45-46
45-65
45-63
45-92
45-93
45-94
E-Line
44-801
44-802
44-803
44-804
44-805
44-807
44-808
44-809
44-812
44-817
44-818
44-819
44-820
44-821
44-825
44-828
44-829
OPTi
Single
38-02
38-02
38-02
38-02
38-04
38-02
38-04
38-02
38-02
P. 33
38-05
38-05
38-06
38-04
38-28
Duo
38-31
38-31
38-31
38-31
38-38
38-31
38-38
38-38
38-38
P. 36
N/A
N/A
38-10
N/A
38-54
+Single
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
38-A1
P. 34
38-A1
38-A1
N/A
38-A1
N/A
+Duo
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
38-B1
P. 37
38-B1
38-B1
38-A3
38-B1
N/A
The instrument selection guide is for general reference only and may not cover all applications or
circumstances. If unsure about an application, please contact our sales department for further
technical advice and assistance.
41
Appendix 2 (continued)
5-25
3-15
3-15
45-01
45-02
45-07
3-18
15-35
45-03
N/A
Fuel systems
Icing inhibitors
3-15
Refractive
index
measurement
5-20
Shampoo
Fire fighting
foam
5-15
Surfactants
detergents &
screen wash
Hydraulic
fluids
5-30
Antifreeze
coolants
15-40
Sodium
chloride
solutions
Beer malts
Wine/cider
alcohol
5-25
Cutting &
quenching oils
38
Seawater
concentrates
5-25
Aquaria/fish
farming
Typical
Brix
Eclipse
Adblue
Model
No.
Starch paper
coating
3-30
45-08
45-05
45-06
45-22
45-27
45-81
45-82
45-91
45-26
45-41
45-44
45-45
45-46
45-65
45-63
45-92
45-93
45-94
E-Line
44-801
44-802
44-803
44-804
44-805
44-807
44-808
44-809
44-812
44-817
44-818
44-819
44-820
44-821
44-825
44-828
44-829
OPTi
Single
38-22
P. 33
38-02
P. 33
38-28
38-26
38-02
38-20
38-02
38-02
38-02
38-02
38-20
Duo
N/A
P. 36
P. 34
38-31
P. 35
38-54
38-51
38-81
P. 35
38-81
38-81
38-31
38-31
P. 35
38-83
N/A
+Single
N/A
N/A
N/A
38-A1
N/A
N/A
N/A
38-A1
N/A
38-A1
38-A1
38-A1
38-A1
38-A2
N/A
+Duo
N/A
N/A
N/A
38-B1
N/A
N/A
N/A
38-B1
N/A
38-B1
38-B1
38-B1
38-B1
38-B1
N/A
The instrument selection guide is for general reference only and may not cover all applications or
circumstances. If unsure about an application, please contact our sales department for further
technical advice and assistance.
42
Appendix 3
Protective Cases for the Eclipse
All Eclipse Refractometers are supplied with a soft case. Spare cases, or cases for use
with E-line models are available.
The Eclipse is an optical instrument, which needs to be handled with care to maintain it in good
working order.
Code
45-97
45-98
Size
Small/Medium
Large
43
Appendix 4
Calibration verification procedure for Hand-Held Refractometers using standard test plates
The calibration of high range hand held refractometers (Eclipse & E-Line models only) can be
verified using any of the standard test plates shown in the table below, together with an appropriate
contact fluid, such as monobromonaphthalene (order code 10-43). Clear, laboratory grade
methylated spirits (methyl alcohol) should be used for removing the test plate and cleaning the
prism.
Test Plate
Silica
Glass
Code No.
72-200
72-207
R.I. @ 20
1.45839
1.48760
Brix @ 20C
67.1
78.8
(19.6% W/H)
44
The calibration of low range Brix/R.I. hand held refractometers can be verified by using a suitable
sucrose solution (see table 1 below). Sucrose solutions are an extremely easy to use medium for
verifying and calibrating refractometers measuring directly in the Brix or Refractive Index (R.I.)
scales.
Sucrose solutions are manufactured on a %weight/weight basis and are calibrated in accordance
with EN ISO/IEC 17025:2005. A number of concentrations are available up to a maximum of 60
Brix, all traceable to NIST and ICUMSA and are supplied with a Certificate of Calibration.
Table 1
Sucrose Standards
Description
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Refractive Index Standard (Sucrose)
Value
(Brix) @ 20C
0.0
5.0
7.5
10.0
11.2
11.5
12.0
12.5
15.0
20.0
25.0
30.0
35.0
40.0
45.0
50.0
55.0
60.0
Value
(R.I.) @ 20C
1.33299
1.34026
1.34401
1.34782
1.34968
1.35015
1.35093
1.35171
1.35568
1.36384
1.37233
1.38115
1.39032
1.39986
1.40978
1.42009
1.43080
1.44193
Order Code
15ml
SS00
SS05
SS075
SS10
SS112
SS115
SS12
SS125
SS15
SS20
SS25
SS30
SS35
SS40
SS45
SS50
SS55
SS60
Table 2
Calibration Oils
Description
Calibration Oil
Calibration Oil
Calibration Oil
Value
(Brix) @ 20C
72.15
91.74
N/A
Value
(R.I.) @ 20C
1.47071
1.52256
1.56138
Order Code
5 x 5ml
90-525
90-530
90-535
Note: The actual R.I./Brix values may vary with different batch numbers, therefore always refer to
the Calibration Certificate that accompanies them. 90-535 calibration oil is not suitable for hand
helds
45
Appendix 5
Temperature Correction Table
This table gives mass fraction corrections to refractometric tables for sucrose solutions at 589nm
for temperature different from 20C. Taken from ICUMSA, Appendix 2, SPS-3 (1998) page 8.
Temp.
(C)
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
15
-0.29 -0.30 -0.32 -0.33 -0.34 -0.35 -0.36 -0.37 -0.37 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.37 -0.37
16
-0.24 -0.25 -0.26 -0.27 -0.28 -0.28 -0.29 -0.30 -0.30 -0.30 -0.31 -0.31 -0.31 -0.31 -0.31 -0.30 -0.30 -0.30
17
-0.18 -0.19 -0.20 -0.20 -0.21 -0.21 -0.22 -0.22 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.22
18
-0.12 -0.13 -0.13 -0.14 -0.14 -0.14 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15
19
-0.06 -0.06 -0.07 -0.07 -0.07 -0.07 -0.07 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.07
20
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
21
0.06
0.07
0.07
0.07
0.07
0.07
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.08
0.07
22
0.13
0.14
0.14
0.14
0.15
0.15
0.15
0.15
0.16
0.16
0.16
0.16
0.16
0.16
0.15
0.15
0.15
0.15
23
0.20
0.21
0.21
0.22
0.22
0.23
0.23
0.23
0.23
0.24
0.24
0.24
0.24
0.23
0.23
0.23
0.23
0.22
24
0.27
0.28
0.29
0.29
0.30
0.30
0.31
0.31
0.31
0.32
0.32
0.32
0.32
0.31
0.31
0.31
0.30
0.30
25
0.34
0.35
0.36
0.37
0.38
0.38
0.39
0.39
0.40
0.40
0.40
0.40
0.40
0.39
0.39
0.38
0.38
0.37
26
0.42
0.43
0.44
0.45
0.46
0.46
0.47
0.47
0.48
0.48
0.48
0.48
0.48
0.47
0.47
0.46
0.46
0.45
27
0.50
0.51
0.52
0.53
0.54
0.55
0.55
0.56
0.56
0.56
0.56
0.56
0.56
0.55
0.55
0.54
0.53
0.52
28
0.58
0.59
0.60
0.61
0.62
0.63
0.64
0.64
0.64
0.65
0.65
0.64
0.64
0.63
0.63
0.62
0.61
0.60
29
0.66
0.67
0.68
0.70
0.71
0.71
0.72
0.73
0.73
0.73
0.73
0.73
0.72
0.72
0.71
0.70
0.69
0.67
30
0.74
0.76
0.77
0.78
0.79
0.80
0.81
0.81
0.82
0.82
0.81
0.81
0.80
0.80
0.79
0.78
0.76
0.75
31
0.83
0.84
0.85
0.87
0.88
0.89
0.89
0.90
0.90
0.90
0.90
0.89
0.89
0.88
0.87
0.86
0.84
0.82
32
0.92
0.93
0.94
0.96
0.97
0.98
0.98
0.99
0.99
0.99
0.99
0.98
0.97
0.96
0.95
0.93
0.92
0.90
33
1.01
1.02
1.03
1.05
1.06
1.07
1.07
1.08
1.08
1.08
1.07
1.07
1.06
1.04
1.03
1.01
1.00
0.98
34
1.10
1.11
1.13
1.14
1.15
1.16
1.16
1.17
1.17
1.16
1.16
1.15
1.14
1.13
1.11
1.09
1.07
1.05
35
1.19
1.21
1.22
1.23
1.24
1.25
1.25
1.26
1.26
1.25
1.25
1.24
1.23
1.21
1.19
1.17
1.15
1.13
36
1.29
1.30
1.31
1.33
1.34
1.34
1.35
1.35
1.35
1.34
1.34
1.33
1.31
1.29
1.28
1.25
1.23
1.20
37
1.39
1.40
1.41
1.42
1.43
1.44
1.44
1.44
1.44
1.43
1.43
1.41
1.40
1.38
1.36
1.33
1.31
1.28
38
1.49
1.50
1.51
1.52
1.53
1.53
1.54
1.54
1.53
1.53
1.52
1.50
1.48
1.46
1.44
1.42
1.39
1.36
39
1.59
1.60
1.61
1.62
1.63
1.63
1.63
1.63
1.63
1.62
1.61
1.59
1.57
1.55
1.52
1.50
1.47
1.43
40
1.69
1.70
1.71
1.72
1.73
1.73
1.73
1.73
1.72
1.71
1.70
1.68
1.66
1.63
1.61
1.58
1.54
1.51
46
Appendix 6
Brix to Refractive Index Conversion Table
The table below can be used to convert Brix readings taken from the instrument scale into
refractive index.
Brix
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
Refractive
Index at
589.3nm and
20.0C
1.33299
1.33442
1.33586
1.33732
1.33879
1.34026
1.34175
1.34325
1.34477
1.34629
1.34782
1.34937
1.35093
1.35250
1.35408
1.35568
1.35729
1.35891
1.36054
1.36218
1.36384
1.36551
1.36720
1.36889
1.37060
1.37233
1.37406
1.37582
1.37758
1.37936
Brix
Refractive Index
at 589.3nm and
20.0C
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
1.38115
1.38296
1.38478
1.38661
1.38846
1.39032
1.39220
1.39409
1.39600
1.39792
1.39986
1.40181
1.40378
1.40576
1.40776
1.40978
1.41181
1.41385
1.41592
1.41799
1.42009
1.42220
1.42432
1.42647
1.42862
1.43080
1.43299
1.43520
1.43743
1.43967
Brix
Refractive Index
at 589.3nm and
20.0C
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
1.44193
1.44420
1.44650
1.44881
1.45113
1.45348
1.45584
1.45822
1.46061
1.46303
1.46546
1.46790
1.47037
1.47285
1.47535
1.47787
1.48040
1.48295
1.48552
1.48811
1.49071
Data Source: ICUMSA Methods Book, Specification and Standard SPS-3 (2000), Refractometry
and Tables - (Official)
47
Appendix 7
% Water in Honey Temperature Correction for 45-27 Eclipse hand held refractometer
Refractive Index is temperature dependant. It is necessary therefore to make corrections for
measurements taken at temperatures other than the reference temperature (20C). For correcting
measurements of Water in Honey the following table may be used. Note that the table shows two
decimal places in order that the value may be rounded up or down accordingly.
Take a reading in accordance with the instruction manual supplied with the instrument.
Correct the scale reading by the correction value obtained from table below. For example, a
sample is measured at 25C and gives a value from the refractometer of 15% Water in Honey.
By using the table below, the corrected reading is: 15 + (-0.44) = 14.56.
Clean the prism with water and soft cloth after each use.
Temperature / C
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
10
0.87 0.88
0.88
0.89
0.89
0.90
0.90
0.90
0.91
0.91
0.92
0.92
0.93
0.93
0.94
0.94
0.95
0.95
0.96
0.96
0.97
11
0.78 0.79
0.79
0.80
0.80
0.81
0.81
0.81
0.82
0.82
0.83
0.83
0.83
0.84
0.84
0.85
0.85
0.86
0.86
0.86
0.87
12
0.70 0.70
0.70
0.71
0.71
0.72
0.72
0.72
0.73
0.73
0.73
0.74
0.74
0.75
0.75
0.75
0.76
0.76
0.76
0.77
0.77
13
0.61 0.61
0.62
0.62
0.62
0.63
0.63
0.63
0.64
0.64
0.64
0.65
0.65
0.65
0.66
0.66
0.66
0.67
0.67
0.67
0.68
14
0.52 0.53
0.53
0.53
0.53
0.54
0.54
0.54
0.55
0.55
0.55
0.55
0.56
0.56
0.56
0.56
0.57
0.57
0.57
0.58
0.58
15
0.44 0.44
0.44
0.44
0.45
0.45
0.45
0.45
0.45
0.46
0.46
0.46
0.46
0.47
0.47
0.47
0.47
0.48
0.48
0.48
0.48
16
0.35 0.35
0.35
0.35
0.36
0.36
0.36
0.36
0.36
0.37
0.37
0.37
0.37
0.37
0.37
0.38
0.38
0.38
0.38
0.38
0.39
17
0.26 0.26
0.26
0.27
0.27
0.27
0.27
0.27
0.27
0.27
0.28
0.28
0.28
0.28
0.28
0.28
0.28
0.29
0.29
0.29
0.29
18
0.18 0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.18
0.19
0.19
0.19
0.19
0.19
0.19
0.19
0.19
0.19
19
0.09 0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.09
0.10
0.10
0.10
0.10
20
0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
21
-0.09 -0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
-0.09
22
-0.17 -0.17
-0.17
-0.17
-0.18
-0.18
-0.18
-0.18
-0.18
-0.18
-0.18
-0.18
-0.18
-0.19
-0.19
-0.19
-0.19
-0.19
-0.19
-0.19
-0.19
23
-0.26 -0.26
-0.26
-0.26
-0.26
-0.27
-0.27
-0.27
-0.27
-0.27
-0.27
-0.28
-0.28
-0.28
-0.28
-0.28
-0.28
-0.28
-0.28
-0.29
-0.29
24
-0.34 -0.35
-0.35
-0.35
-0.35
-0.36
-0.36
-0.36
-0.36
-0.36
-0.37
-0.37
-0.37
-0.37
-0.37
-0.37
-0.38
-0.38
-0.38
-0.38
-0.38
25
-0.43 -0.43
-0.44
-0.44
-0.44
-0.44
-0.45
-0.45
-0.45
-0.45
-0.46
-0.46
-0.46
-0.46
-0.47
-0.47
-0.47
-0.47
-0.47
-0.48
-0.48
26
-0.52 -0.52
-0.52
-0.53
-0.53
-0.53
-0.54
-0.54
-0.54
-0.54
-0.55
-0.55
-0.55
-0.56
-0.56
-0.56
-0.56
-0.57
-0.57
-0.57
-0.57
27
-0.60 -0.61
-0.61
-0.61
-0.62
-0.62
-0.63
-0.63
-0.63
-0.64
-0.64
-0.64
-0.65
-0.65
-0.65
-0.66
-0.66
-0.66
-0.66
-0.67
-0.67
28
-0.69 -0.69
-0.70
-0.70
-0.71
-0.71
-0.71
-0.72
-0.72
-0.73
-0.73
-0.73
-0.74
-0.74
-0.75
-0.75
-0.75
-0.76
-0.76
-0.76
-0.77
29
-0.78 -0.78
-0.79
-0.79
-0.80
-0.80
-0.80
-0.81
-0.81
-0.82
-0.82
-0.83
-0.83
-0.83
-0.84
-0.84
-0.85
-0.85
-0.85
-0.86
-0.86
30
-0.86 -0.87
-0.87
-0.88
-0.88
-0.89
-0.89
-0.90
-0.90
-0.91
-0.91
-0.92
-0.92
-0.93
-0.93
-0.94
-0.94
-0.94
-0.95
-0.95
-0.96
31
-0.95 -0.96
-0.96
-0.97
-0.97
-0.98
-0.98
-0.99
-0.99
-1.00
-1.00
-1.01
-1.01
-1.02
-1.02
-1.03
-1.03
-1.04
-1.04
-1.05
-1.05
32
-1.04 -1.04
-1.05
-1.05
-1.06
-1.07
-1.07
-1.08
-1.08
-1.09
-1.09
-1.10
-1.11
-1.11
-1.12
-1.12
-1.13
-1.13
-1.14
-1.14
-1.15
33
-1.12 -1.13
-1.14
-1.14
-1.15
-1.15
-1.16
-1.17
-1.17
-1.18
-1.19
-1.19
-1.20
-1.20
-1.21
-1.22
-1.22
-1.23
-1.23
-1.24
-1.24
34
-1.21 -1.22
-1.22
-1.23
-1.24
-1.24
-1.25
-1.26
-1.26
-1.27
-1.28
-1.28
-1.29
-1.30
-1.30
-1.31
-1.32
-1.32
-1.33
-1.33
-1.34
35
-1.29 -1.30
-1.31
-1.32
-1.32
-1.33
-1.34
-1.35
-1.35
-1.36
-1.37
-1.37
-1.38
-1.39
-1.40
-1.40
-1.41
-1.42
-1.42
-1.43
-1.44
36
-1.38 -1.39
-1.40
-1.40
-1.41
-1.42
-1.43
-1.44
-1.44
-1.45
-1.46
-1.47
-1.47
-1.48
-1.49
-1.50
-1.50
-1.51
-1.52
-1.52
-1.53
37
-1.47 -1.48
-1.48
-1.49
-1.50
-1.51
-1.52
-1.53
-1.53
-1.54
-1.55
-1.56
-1.57
-1.57
-1.58
-1.59
-1.60
-1.60
-1.61
-1.62
-1.63
38
-1.55 -1.56
-1.57
-1.58
-1.59
-1.60
-1.61
-1.61
-1.62
-1.63
-1.64
-1.65
-1.66
-1.67
-1.67
-1.68
-1.69
-1.70
-1.71
-1.71
-1.72
39
-1.64 -1.65
-1.66
-1.67
-1.68
-1.69
-1.69
-1.70
-1.71
-1.72
-1.73
-1.74
-1.75
-1.76
-1.77
-1.78
-1.78
-1.79
-1.80
-1.81
-1.82
40
-1.72 -1.73
-1.74
-1.75
-1.76
-1.77
-1.78
-1.79
-1.80
-1.81
-1.82
-1.83
-1.84
-1.85
-1.86
-1.87
-1.88
-1.89
-1.89
-1.90
-1.91
Table is based upon the temperature coefficient of -0.00023 R.I./C given in the following documents:
AOAC Official Method 969.38 - Moisture In Honey.
AOAC Official Methods of Analysis (2000)
48
Appendix 8
Extended ABV table 2 decimal resolution %ABV
R-D
%v/v Alc
R-D
%v/v Alc
R-D
%v/v Alc
15.0
15.5
16.0
16.5
17.0
17.5
18.0
18.5
19.0
19.5
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
24.0
24.5
25.0
25.5
26.0
26.5
27.0
27.5
28.0
28.5
29.0
29.5
30.0
30.5
31.0
31.5
32.0
32.5
33.0
33.5
34.0
34.5
35.0
35.5
36.0
36.5
37.0
37.5
38.0
0.08
0.27
0.45
0.63
0.82
1.00
1.18
1.37
1.55
1.73
1.92
2.10
2.29
2.47
2.65
2.84
3.02
3.20
3.39
3.57
3.75
3.94
4.12
4.30
4.49
4.67
4.86
5.04
5.22
5.41
5.59
5.77
5.96
6.14
6.32
6.51
6.69
6.87
7.06
7.24
7.43
7.61
7.79
7.98
8.16
8.34
8.53
38.5
39.0
39.5
40.0
40.5
41.0
41.5
42.0
42.5
43.0
43.5
44.0
44.5
45.0
45.5
46.0
46.5
47.0
47.5
48.0
48.5
49.0
49.5
50.0
50.5
51.0
51.5
52.0
52.5
53.0
53.5
54.0
54.5
55.0
55.5
56.0
56.5
57.0
57.5
58.0
58.5
59.0
59.5
60.0
60.5
61.0
61.5
8.71
8.89
9.08
9.26
9.44
9.63
9.81
10.00
10.18
10.36
10.55
10.73
10.91
11.10
11.28
11.46
11.65
11.83
12.01
12.20
12.38
12.56
12.75
12.93
13.12
13.30
13.48
13.67
13.85
14.03
14.22
14.40
14.58
14.77
14.95
15.13
15.32
15.50
15.69
15.87
16.05
16.24
16.42
16.60
16.79
16.97
17.15
62.0
62.5
63.0
63.5
64.0
64.5
65.0
65.5
66.0
66.5
67.0
67.5
68.0
68.5
69.0
69.5
70.0
70.5
71.0
71.5
72.0
72.5
73.0
73.5
74.0
74.5
75.0
75.5
76.0
76.5
77.0
77.5
78.0
78.5
79.0
79.5
80.0
80.5
81.0
81.5
82.0
82.5
83.0
83.5
84.0
84.5
17.34
17.52
17.70
17.89
18.07
18.26
18.44
18.62
18.81
18.99
19.17
19.36
19.54
19.72
19.91
20.09
20.27
20.46
20.64
20.83
21.01
21.19
21.38
21.56
21.74
21.93
22.11
22.29
22.48
22.66
22.84
23.03
23.21
23.40
23.58
23.76
23.95
24.13
24.31
24.50
24.68
24.86
25.05
25.23
25.41
25.60
49
Appendix 9
Calibration & verification points for hand held refractometers
Model
45-01
45-02
45-03
45-05
45-06
45-07
45-08
45-22
45-26
45-27
45-41
45-44
45-45
45-46
45-63
45-65
45-81
45-82
45-91
45-92
45-93
45-94
44-801
44-802
44-803
44-804
44-805
44-808
44-809
44-807
44-806
44-812
44-817
44-818
44-819
44-820
SS00
SS00
SS00
SS00
44-821
44-825
SS00
44-828
44-829
Scale
0 - 15% sugar
0 - 30% sugar
0 - 50% sugar
45-80% sugar
72-95% sugar
0-32% sugar
28-65% sugar
10-135 Zeiss
0-30% starch
10-30 W/H
1.33-1.42 RI
0 to -40C EG/PG
30 to -30F EG/PG
0-60% vol PG/EG
0-180 PPT
0-28% NaCl
0-50% sugar
45-80% sugar
0-30% sugar ATC
0-16 Baume/0-18AP
0-130 Oe/0-30% sugar
30-130 O/0-30% sugar
0-10% sugar
0-18% sugar
0-32% sugar
28-62% sugar
45-82% sugar
0-100 PPT
0-40% sugar/0-20AP
0-80% sugar
0-90% sugar
10-30 W/H
0-32% sugar/0-140 OeD
0-32% sugar/0-140 Oe
0-20 Baume/0-25 AP
0 to -50C EP-PG/0-70% EP-PG
0 to -50C EP-PG/1.10 to 1.40 SG
0 to -40C screenwash
0-12 Serum Protein/1.335-1.360 RI
1.000 - 1.050 Urine SG
1.30 - 1.38 R.I.
1.435 - 1.520 R.I.
Notes
Please refer to tables 1 & 2 on page 38 for full descriptions on these calibration standards
Due to dispersion reasons a monochromic light source should be adopted when using the
90-525 and 90-530 calibration oils
All values are calculated based on the sample and instrument temperatures are at 20C.
50
Appendix 10
The FAQ listed below are available from the new Hand Held Distribution Channel Resource
Centre of our website:
www.bellinghamandstanley.com/HHDCRC
FAQ Number
Type
FAQ-HH-001
Technical
FAQ-HH-008
FAQ-HH-009
FAQ-HH-010
Commercial
Technical
Application
FAQ-HH-011
Technical
FAQ-HH-012
Technical
FAQ-HH-014
Application
FAQ-HH-015
FAQ-HH-016
FAQ-HH-018
FAQ-HH-019
FAQ-HH-020
FAQ-HH-021
FAQ-HH-022
FAQ-HH-024
FAQ-HH-025
Technical
Application
Application
Application
Application
Application
Technical
Application
Application
FAQ-HH-026
Technical
FAQ-HH-027
Technical
FAQ-HH-028
FAQ-HH-029
FAQ-HH-030
Technical
Technical
Technical
FAQ-HH-031
Commercial
FAQ-OPT-001
FAQ-OPT-002
FAQ-OPT-003
FAQ-OPT-004
FAQ-OPT-005
FAQ-OPT-009
Technical
Technical
Technical
Commercial
Commercial
Commercial
Title
Calibrate a HIGH RANGE hand held refractometer without using
water (i.e. 45-80% Sugar Water-in-Honey)
Eclipse Advantages over competitor instruments
Illuminator Flap chemical resistance & material
APPLICATION: Water soluble carbohydrate and protein in grass
STATEMENT: Urine Specific Gravity Measurement by Eclipse
Refractometer (IVD regulation 98/79)
CE mark
Determination of ABV (proof) of liquors and spirits by hand
refractometer
Conversion Table Brix to Specific Gravity, g/100ml (Urine)
APPLICATION: Horse Breeding - Equine Colostrum Quality
APPLICATION: lactose by hand refractometer
APPLICATION: Measuring root vegetables with a hand refractometer
Water-in-Honey: an explanation about use of a refractometer
APPLICATION: Hand refractometers in the aviation industry
IP Rating Guide
Measuring Salinity (sea-water aquariums fish farm lagoons)
APPLICATION: Analysis of whole egg
Create a User Defined Table for a Brix Refractometer (i.e.
lubricant/surfactant etc.)
NOx reduction agent AUS 32 (Adblue) Brix to %Concentration
Conversion Chart
Extended ABV table 2-decimal
Difficult samples tips
DEF (Diesel Exhaust Fluid) Hydrometer User Guide
ABV Internet Calculator Instructions/Disclaimer (PC and Mobile
platforms)
Common Specifications
Intrinsic Safety
Service & Certification
10 Selling Points
Custom Models
OPTi - Definitive Model List at July 2012 (OPTi+ launch)
51
International
Longfield Road
Tunbridge Wells, Kent
TN2 3EY
United Kingdom
USA
1000 Hurricane Shoals Road
Building D, Suite 300
Lawrenceville, GA30043
United States of America
www.bellinghamandstanley.com
2012 Xylem, INC. All rights reserved. Bellingham + Stanley, OPTi, OPTi+, Eclipse & e-line are trade marks of Xylem, Inc. or one of its subsidiaries.
52