Dah er eiuteungesee eee Due: MICROSCOPE #3: Weft Mounts 1) Learn to prepare WET MOUNTS, temporary slides using water to hold the slide together. Use the technique shown in the accompanying diagrams while you make the slides and observations for this lab. coversilp 1 Add a drop of water to the center of a clean, dry slide. +{ Cut out a small letter "e" from the newspaper classifieds. | Place the “e" into the water drop using forceps or probe. \{ Position the cover slip next to the water drop at an angle. This should reduce the chance of trapping air bubbles. Air bubbles will appear as dark rings in the sample. D 2) Mount your slide on the stage right-side-up, as if you were going to read it normally. Observe under low power, and sketch is AS YOU SEEIT. Your drawing goes in the space below: Thewsprint letter "e" total low power magnification = 3) Use the information you learned on the previous lab about measurement to determine the diameter of your letter "e.” Record your answer here: e a ae4) Make a wet mount of a few COTTON FIBERS. | Center some "good" fibers by moving the slide as you observe under LOW POWER «| Move the slide slightly left and right and forward and backward. Be prepared to compare these movements of the fibers as seen through the eyepiece to the movement of the slide. 1 Focus first with COARSE ADJUSTMENT and then use FINE ADJUSTMENT | Sketch these fibers under low power. Be sure to indicate total magnification. cotton fibers - low power cotton fibers - high power total low power magnification = total high power magnification J Change to the high power objective, and draw a few fibers. Do this by: a) Center some of the fibers in your low power view. b) While looking from the side of the stage, rotate the nosepiece to turn the high power objective into position. It will click into place. ©) Look through the eyepiece. d) Turn the fine adjustment to bring the fibers into focus. Questions to Answer: Use blue or black ink and SENTENCES unless otherwise directed. . Inpreparing a wet mount of onion cells for observation with a compound light microscope, a student placed a small piece of onion epidermis in a drop of water on a clean slide. Next he added a clean cover slip. He then placed the slide on the stage of the microscope and focused under high power. Using one or more complete sentences, state an error in the student's procedure.3. 2 Astudent changes the objective of a microscope from 10X to SOX. If this is the only change made, what will happen to the field of view? A. Its diameter will decrease. B. Its diameter will increase C. Its brightness will increase. D. Its brightness will remain the same. Using your own words, explain the difference between MAGNIFICATION and RESOLUTION (resolving power) ina compound microscope. {See pages 22231 ___ 4. The field of view becomes darker when a compound microscope is switched a from low to high power. The field of view can then be made brighter by ‘A, decreasing the size of the diaphragm opening 8. increasing the size of the diaphragm opening C. refocusing with the fine adjustment D. refocusing with the coarse adjustment Explain why you made that choice. While observing a specimen mounted on a slide under a microscope, a student moves the slide in the direction of arrow A. - ~ VA B In which direction will the er owe @ seen through the : : microscope? Circle your choice on the diagram. Explain why you made that choice.. Why musta specimen to be viewed under the microscope be thin? Print your name as it would appear through the microscope: What is the danger of using coarse adjustment on high power? . Figure A represents a cell as viewed by a student using the 10X ocular and the 10X objective of a compound light microscope. Figure B represents the same cell as seen with a different objective and using the same 10X ocular. Figure A Figure B Circle the power of the objective used to observe the cell shown in figure B is most likely 1) 4X 2) 40X 3) 60X 4) 100X Explain why you made that choice.