Introduction

Ganoderma lucidum, a fungus that belongs to the family of polyporaceae is

one of the most well-known traditional medicinal mushrooms and has been widely
used in Asian countries for many years. It is well known as Lingzhi in Chinese,
Reishi in Japanese, and Youngzhi in Korean. The extracts from this mushroom
have been intensively studied because of their wide range of biological activities,
such as anti-cancer, antiallergy, anti-inflammatory, antioxidant, blood pressure
reducing, hypoglycemic effects, and platelet-aggregation inhibiting activities.
Naturally, these plants grow on wood or the trees and commonly can be found in the
mountains with high humidity and fuzzy.

In general, the cultivation of Ganoderma lucidum can be carried out in both

solid-state and submerged cultivation. However, submerged cultivation is more
preferable since it produces high yield of particular products and saves time. Instead,
it usually takes several months to cultivate the fruiting body of Ganoderma lucidum
and it is also difficult to control the product quality. Currently, the mycelial biomass,
extra-cellular polysaccharide (EPS) and intra-cellular polysaccharides (IPS) are three
of the most desired products because of its perceived health benefits. Instead,
cellulolytic enzymes activity of Ganoderma lucidum is also widely studied.

In recent years, submerged cultivation of Ganoderma lucidum has received

great interest in Asian countries as a promising alternative for efficient production of
its valuable metabolites. However, the role of different factors in their production
under the conditions of submerged culture is poorly understood. Thus, this study can
enhance the understanding about the effects of several factors on the production of
Ganoderma lucidum and its metabolites under submerged cultivation condition
specifically in term of substrates and types of carbon source effects. As the matter of
fact, the suitable type of carbon sources and substrates gradually increase the cell
growth and led to the highest production of cell biomass, polysaccharides (EPS and
IPS) and cellulase enzymes of Ganoderma lucidum.

Hence, the goal of this work was to study the best carbon sources used and
the effects of types of carbon sources on the cell growth, the production of cell
biomass and polysaccharides of Ganoderma lucidum. Furthermore, this research was
studied about the effects of substrates variability on the production of cellulase
enzyme in order to determine the best substrate for the production of the cellulase
enzymes.