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  • Gas Chromatography

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    Sukumar Barik
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    This document discusses gas chromatography techniques including column resolution, sample injection systems, rotary sample valves, column configurations, temperature programming, and choices for stationary phases. It summarizes key aspects of gas chromatography from Chapter 26 of the textbook by Skoog, Holler, and Niemen, including that sample injection must be slow to avoid band spreading, common column lengths are 2-50m, and temperature programming is used to separate compounds by gradually increasing the column temperature.

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    analytical instrumentation

    Original Title

    Lecture 10

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    This document discusses gas chromatography techniques including column resolution, sample injection systems, rotary sample valves, column configurations, temperature programming, and choices for stationary phases. It summarizes key aspects of gas chromatography from Chapter 26 of the textbook by Skoog, Holler, and Niemen, including that sample injection must be slow to avoid band spreading, common column lengths are 2-50m, and temperature programming is used to separate compounds by gradually increasing the column temperature.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    10 views12 pages

    Gas Chromatography

    Uploaded by

    Sukumar Barik
    This document discusses gas chromatography techniques including column resolution, sample injection systems, rotary sample valves, column configurations, temperature programming, and choices for stationary phases. It summarizes key aspects of gas chromatography from Chapter 26 of the textbook by Skoog, Holler, and Niemen, including that sample injection must be slow to avoid band spreading, common column lengths are 2-50m, and temperature programming is used to separate compounds by gradually increasing the column temperature.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 12

    Lecture 10

    Gas Chromatography

    Column Resolution

    Skoog, Holler, Niemen, Chapter 26

    Summary The General


    Elution Problem

    Skoog, Holler, Niemen, Chapter 26

    Skoog, Holler, Niemen, Chapter 26

    InstrumentationBasic Gas Chromatography

    Skoog, Holler, Niemen, Chapter 26

    Sample Injection System


    -Liquid or Gas
    -Important! Slow injection causes band spreading,
    poor resolution
    -Sample is 0.1 to 20 microliters, typical packed
    column GC or.0001 microliters for capillary GC

    Skoog, Holler, Niemen, Chapter 26

    Rotary Sample Valve


    A. Fill sample loop

    Skoog, Holler, Niemen, Chapter 26

    Column
    Configurations

    Length 2-50 m

    1. Packed

    2. Open tubular or capillary

    3. Temperature

    Skoog, Holler, Niemen, Chapter 26

    Temperature
    Programming
    45 C

    145 C

    30-180 C

    Many choices for Stationary Phase


    (for packed and tubular columns)

    Many Choices!

    Open Tubular Columns

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