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Gen Exam 4
Gen Exam 4
1) Dengue fever
a. Is caused by infected birds
b. Is responsible for influenza
c. Is confined to the tropics because its non-human host cannot survive at low temperatures
d. Is spreading into temperate zones because of climate changes
2) Suppose you find in a forensic analysis that a crime sample matches all 14 of the standard DNA polymorphisms of
a suspect. (That is, the genotype is exactly the same at all 14 standard polymorphic loci). To calculate the chance that
this is a coincidence, i.e., the result of a random match, what information do you need?
a. Additional tests of more polymorphic loci
b. The entire DNA sequence of both the crime sample and the suspect.
c. The frequency of each of these polymorphic alleles in the population.
d. None of the above
3) Suppose you want to clone a bacterial gene in yeast. Which of the following steps do you NOT have to do?
a. Isolate mRNA from the bacteria
b. Mix the bacterial gene with vector DNA
c. Transform the yeast with recombinant DNA
d. Screen yeast cells for the bacterial gene sequence.
4) The concept of alleles has been extended to noncoding sequences in DNA, such as STR sequences. These alleles
can be tracked in pedigrees and compared with ____________ in order to identify genes that cause disease.
a. records of crossing over
b. DNA sequences of normal genes
c. health records in the pedigree
d. All of the above
5) In the lac operon in E. coli, covered in class, suppose there is a mutation that completely deletes the operator
sequence. In this mutant, which of the following characteristics would be found?
a. the cell cannot use lactose as nutritional source.
b. the cell is less efficient than normal at using glucose and lactose
c. the cell is always making enzymes for the utilization of lactose, even when no lactose is available
d. the cell is always making enzymes for the utilization of glucose, except when lactose is available
6) A sequence in a certain stretch of DNA is
5P-AGTCCGG-3OH.
The RNA transcribed from this template would therefore have the sequence
a. 5-TCAGGCC-3OH
b. 5-CCGGACU-3OH
c. 5-AGUGGCC-3OH
14) In order to match the pilot's remains to the correct family using DNA profiling,
a. the majority of the STR bands must match.
b. each of the 13 STR bands must match.
c. the bands for site 13 must match.
d. bands 5 and 7 must match.
15) Based on analysis of the STR sites shown, does the missing pilot belong to any of these three families?
a. No, none of the families match.
b. Yes, family 1 matches.
c. Yes, family 2 matches.
d. Yes, family 3 matches.
Essay questions:
2. The largest and the most frequent new disease outbreaks are recorded for Europe and North
America. But the majority of new diseases arise by transfer of infectious agents from animals to
people, in other locations often where people live in closer proximity to their farm animalstypically from rural and/or third world countries. How do you reconcile these two diverse
observations concerning the source of an emerging infectious disease and the location of its
greatest impact? (4 points)