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Albumin: Colorimetric Method
Albumin: Colorimetric Method
ALBUMIN
in vitro DIAGNOSTIC REAGENT KIT
Colorimetric method
Cat. No: 35011-2-99-80
Package: 2 x 500 ml
Wavelength:
Hg 578 nm or Hg 623 nm
Spectrophotometer: 623 nm
Light path: 1 cm
Temperature: +20 - +25 C
Procedure
Method
Albumin forms a coloured complex with bromcresol
green at pH 3.9. The absorbance of this complex is
measured.
Reagents
1. Buffer/Chromogen solution
Succinate buffer
pH 3.9
Bromcresol green
375 mmol/l
1 mmol/l
2. Standard
Albumin
Standard concentration is stated on the label
Storage, stability
The kit is stable up to the expiry date stated when stored
at +2 - +8 C.
Reference
Doumas, B.T., Watson, W.A., Biggs, H.G.: Clin. Chim. Acta
31, 87 (1971)
Calculation
Albumin(g/l) =
A sample
x Standard concentration
A standard
Reagent solution
The Buffer/Chromogen solution (1.) is filled up to
500 ml with redistilled water and mixed well.
Stable for
3 months stored at +2 - +8 C.
Standard solution
Normal value
Serum
Sample
Serum, EDTA plasma
Stable
for 6 days at +2 - +8 C.
38 - 50 g/l
Quality control
Any control serum applicable to this method.
Recommended:
Reanorm U
Reapath U
REA-kon I.
REA-kon II.
Reg.No.:HU/CA01/2340/05
ALBUMIN
in vitro DIAGNOSZTIKAI REAGENS KSZLET
Kolorimetris mdszer
Cikkszm: 35011-2-99-80
Kiszerels: 2 x 500 ml
Mdszer
Az albumin a brmkrezolzlddel pH 3,9-nl sznes
komplexet kpez, amelynek abszorbancijt fotometrisan mrjk.
Reagensek
Mrs
Hullmhossz :
Eljrs
Irodalom
Kmcsvekbe pipettzunk
Reagens
Standard
Minta
vak
Munkaoldat
3,00 ml
3,00 ml
3,00 ml
Standard
0,02 ml
Minta
0,02 ml
sszekeverjk, majd +20 - +25 C-on 15 percig llni
hagyjuk. 1 rn bell reagens vakkal szemben leolvassuk az abszorbancit.
Szmols
Munkaoldatok ksztse
Linearits
Albumin (g/l) =
Doumas B.T., Watson W.A., Biggs H. G.: Clin. Chim. Acta 31,
87 (1971)
minta
x standard koncentrci
standard
Standard oldat
Trols, stabilits
Fnyt : 1 cm
Hmrsklet : +20 - +25 C
1. Puffer/Sznkpz oldat
pH 3,9
Na-szukcint puffer
375 mmol/l
brmkrezolzld
1 mmol/l
2. Standard
liofilezett albumin
Standard koncentrci a cmkn van feltntetve.
Munkaoldat
38 - 50 g/l
Minsgi ellenrzs
Minden kontroll szrummal, amely ezt a mdszert
alkalmazza.
Ajnlott : Reanorm U
Reapath U
REA-kon I.
REA-kon II.
Reg.sz.: HU/CA01/2340/05