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Pharmacognostical & Phyto-Chemical Investigation of Cissus Quadrangularis Linn. Stem PDF
Pharmacognostical & Phyto-Chemical Investigation of Cissus Quadrangularis Linn. Stem PDF
: IJPRD/2011/PUB/ARTI/VOV-3/ISSUE-1/MARCH/020
Anuj Srivastava
ABSTRACT
Cissus quadrangularis (Family-Vitaceae) is found in hotter parts of India and neibouring countries
Pakistan, Sri Lanka, and Malaysia .Stem of the plant are used as anthelmintic, laxative, chronic ulcer,
,analgesics and in Bone healing activity. The Stem of Cissus quadrangularis was collected and
powdered. Ethyl acetate extract was prepared by using Cold Maceration and subsequently fractioned
with hydro alcoholic (50:50) for detailed chemical analysis. Fluorescence characters of different
Macerated extract and powder were noted under ultra violet light and under normal ordinary light, which
signifies their characteristics. The total ash value, acid insoluble ash value and water soluble ash value
were 12.5%, 2.5% & 10.0%, respectively. The percentage yields for ethyl acetate, Hydro alcoholic were
2% and 1.8%. respectively. Reaction behaviors of powder stem of Cissus quadrangularis with 10
different reagents were also noted, which revealed characteristic development of particular color.
Preliminary qualitative chemical tests of extracts were found positive results for Phytosterol, flavonoids
and triterpenoids in Ethyl acetate fraction, Hydro alcoholic fraction was positive to carbohydrate, tannin
and amino acid and vitamin C. Flavonoids and triterpenoids are the active constituents in stem of
Cissus quadrangularis and may be responsible for its pharmacological activities. The results of the
study could be useful in setting some diagnostic indices for the identification and preparation of a
monograph of the plant.
Key Words: Cissus quadrangularis, Maceration, Physicochemical analysis
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http://botanical.com/site/column_poudhia/121_b
hopalpatnam.html.
4. Kokate, C.K., Practical Pharmacognosy, 4th
edn, Vallabh Prakashan, Delhi, 1994, pp 15-29
5. Ayurvedic Pharmacopoeia of India, 1986.
The Ministry of Health and Family Welfare.
Govt. of India, New Delhi, Vol III, Third edition,
pp. 21-22.
6.
Khandelwal, K.R., 2005. Practical
Pharmacognosy,
fourth
edition,
Vallabh
Prakashan, New Delhi; p.p. 145.
Flower
Fruit
Table 2:
Features
Nature
Colour
Odour
Taste
Observation
Coarse powder
Cream colour light yellow
Slightly characteristic
Slightly characteristic
Present
Present
Collenchymas cell
Phloem fiber
Raphides and Acicular
Crystals of calcium oxalate
Fibres
Pitted Parenchymatous cells
Present
Present
Present
Present
Present
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Table 3:
Total ash
12.5
2.5
10.0
Sulphated ash
5.0
Extractive values
Water soluble extractive
18.0
3.0
2.0
Colour observed
Powder as such
Whitish yellow
Brown
Yellow
Brown
Powder + 5% NaOH
Brown
Powder + 5% KOH
Yellowish brown
Light yellow
Yellow
Powder + ammonia
Light brown
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Long Wavelength
Cream
Brown
Brown
Reddish brown
Green
Green
Dark green
Light brown
Reddish brown
Brown
Brown
Dark brown
colorless
Yellow
Green
Brown
Yellowish brown
Reddish brown
Greenish brown
Colorless
Yellowish green
Black
Light brown
Reddish brown
Powder
Drug
Ethyl
acetate
Extract
Hydro
alcoholic
Extract
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
Xanthoprotic Test
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Spot Test
Saponofication Test
Legals test
Balgets test
Borntragers test
Liebermanns Test
+++
++
++
Salkowski Test
+++
++
+++
Fluorescence Test
++
+++
+++
Shinoda Test
++
+++
++
++
Haemolysis
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End
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