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  • Penentuan Kadar Protein (Metode Lowry)

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    Lia Yusliawati
    11 views4 pages
    This document contains the results of a protein concentration determination experiment using the Lowry method. Absorbance readings were taken for BSA protein standards at concentrations ranging from 0-1000 ppm to generate a calibration curve. The absorbance of an unknown sample was then measured and used to determine its protein concentration through interpolation of the calibration curve equation. Sample concentrations of 752 ppm and 471.67 ppm were calculated for two unknown samples based on their absorbance values and the linear trendlines of the calibration curves.

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    biokimia

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    This document contains the results of a protein concentration determination experiment using the Lowry method. Absorbance readings were taken for BSA protein standards at concentrations ranging from 0-1000 ppm to generate a calibration curve. The absorbance of an unknown sample was then measured and used to determine its protein concentration through interpolation of the calibration curve equation. Sample concentrations of 752 ppm and 471.67 ppm were calculated for two unknown samples based on their absorbance values and the linear trendlines of the calibration curves.

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    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as xlsx, pdf, or txt
    11 views4 pages

    Penentuan Kadar Protein (Metode Lowry)

    Uploaded by

    Lia Yusliawati
    This document contains the results of a protein concentration determination experiment using the Lowry method. Absorbance readings were taken for BSA protein standards at concentrations ranging from 0-1000 ppm to generate a calibration curve. The absorbance of an unknown sample was then measured and used to determine its protein concentration through interpolation of the calibration curve equation. Sample concentrations of 752 ppm and 471.67 ppm were calculated for two unknown samples based on their absorbance values and the linear trendlines of the calibration curves.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as xlsx, pdf, or txt
    of 4

    ABSORBANSI

    BSA
    0.105
    0.177
    0.187
    0.277
    0.343
    0.394
    0.61
    KONSENTRASI ABSORBANSI
    X
    Y

    Y (ABSORBANSI-BLANKO)
    0
    0.1
    0.2
    0.4
    0.6
    0.8
    1

    0.072
    0.01
    0.09
    0.066
    0.051
    0.216

    BLANKO
    100
    200
    400
    600
    800
    1000

    0.072
    0.082
    0.172
    0.238
    0.289
    0.505 r
    SLOFT/GRADIEN (M)
    INTERSECT
    Y=MX+C
    y=sampel tabung 8

    0.96
    0.0004434521
    -0.0027835616
    Y=0.0004x - 0.0028
    0.298=0.0004x - 0.0028
    0.298 + 0.0028=0.0004x
    x=0.3008/0.0004
    x=752

    PENENTUAN KADAR PROTEIN (METODE LOWRY)


    0.6
    0.505

    0.5
    0.4

    ABSORBANSI

    0.3

    f(x) = 0.0004434521x - 0.0027835616 0.289


    R = 0.9250490972

    0.2
    0.1 0.072
    0
    0
    100

    0.082

    200

    0.172

    300

    400

    0.238

    500

    600

    700

    800

    KONSENTRASI (ppm)
    Column B

    Linear (Column B)

    900 1000 1100

    =0.0004x

    Y
    100
    200
    400
    600
    800
    1000

    0.454
    0.673
    0.676
    0.782
    0.799
    0.806

    ABSORBANSI
    BSA
    0.105
    0.559
    0.778
    0.781
    0.887
    0.904
    0.911

    Y (ABSORBANSI-BLANKO)
    0
    0.1
    0.2
    0.4
    0.6
    0.8
    1

    BLANKO

    r
    SLOFT/GRADIEN (M)
    INTERSECT
    Y=MX+C
    y=sampel tabung 8

    0.86
    0.0003287123
    0.5284986301
    Y=0.0003x + 0.5285
    0.670=0.0003x + 0.5285
    0.670 - 0.5285=0.0003x
    x=0.1415/0.0003
    x=471.67

    PENENTUAN KADAR PROTEIN (METODE LOWRY)


    1

    0.806
    0.799
    f(x) = 0.0003287123x + 0.782
    0.5284986301
    0.676
    0.673
    R = 0.7338945352

    0.8
    0.6

    ABSORBANSI

    0.4

    0.454

    0.2
    0
    0

    100

    200

    300

    400

    500

    600

    700

    800

    KONSENTRASI (ppm)
    Column B

    Linear (Column B)

    900

    1000 1100

    x + 0.5285
    =0.0003x

    RY)

    00

    0.806

    1000 1100

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