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  • What Is The Principle of HPLC?

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    HPLC separates compounds based on their interactions with a nonpolar stationary phase and a polar mobile phase. Reverse phase HPLC uses a nonpolar stationary phase like C18 and a mobile phase that is a water-organic solvent mixture. Compounds are separated based on their relative nonpolarity, with nonpolar compounds retained longest by the stationary phase.

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    HPLC separates compounds based on their interactions with a nonpolar stationary phase and a polar mobile phase. Reverse phase HPLC uses a nonpolar stationary phase like C18 and a mobile phase that is a water-organic solvent mixture. Compounds are separated based on their relative nonpolarity, with nonpolar compounds retained longest by the stationary phase.

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    5 views1 page

    What Is The Principle of HPLC?

    Uploaded by

    zzatiee
    HPLC separates compounds based on their interactions with a nonpolar stationary phase and a polar mobile phase. Reverse phase HPLC uses a nonpolar stationary phase like C18 and a mobile phase that is a water-organic solvent mixture. Compounds are separated based on their relative nonpolarity, with nonpolar compounds retained longest by the stationary phase.

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    hplc

    HPLC Operation

    What is the principle of HPLC?


    Reverse Phase HPLC: The stationary phase is nonpolar (hydrophobic) in nature, while
    the mobile phase is a polar liquid, such as mixtures of water and methanol or
    acetonitrile. It works on the principle of hydrophobic interactions hence the more
    nonpolar the material is, the longer it will be retained.

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