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THE UNITED STATES OF AMERICA

Legally Binding Document

By the Authority Vested By Part 5 of the United States Code 552(a) and
Part 1 of the Code of Regulations 51 the attached document has been duly
INCORPORATED BY REFERENCE and shall be considered legally
binding upon all citizens and residents of the United States of America.
HEED THIS NOTICE: Criminal penalties may apply for noncompliance.

e
Document Name:

ALCIDE 980342EA: Determination of Sodium

Chlorite: 50 ppm to 1500 ppm concentration

CFR Section(s):

21 CFR 173.325(g)

Standards Body:

Alcide Corporation

Official Incorporator:
THE EXECUTIVE DIRECTOR

OFFICE OF THE FEDERAL REGISTER

WASHINGTON, D.C.

Determination of Sodium chlorite:

50 PPM to 1500 PPM Concentration

Alcide Corporation
September 13, 1995

7?F-03cf2-

Eli- /

ALCIDB CORPORATION
LABORATORY TEST METHOD

, H'CMBBR. : 1124
I REV: 01

DETERMINATION OF SODIUM CHLORITE:

50 PPM TO 1500 PPM CONCENTRATION

,PAGE: 1 of 2

QUALITY ASSTJRANC3:
TITLE z

IAPPR.O~ BY:
1

,/Yt1

(~

I BPI". DATE:
I 09/13/95

SUPBR.SBDES :
I

05/17/95

1.00 Scope

Solutions containing sodium chlorite at 50-1500 ppm can be

assayed for sodium chlorite concentration by titration of the
iodine released in the presence of potassium iodide and
hyrochloric acid.
2.00 Equipment
250 mL Erlenmeyer flasks
Stir bars and magnetic stirrer
50 mL buret
Buret stand
Buret clamp
100 mL graduated cylinders/ or balance capable of weighing 250g
3.00 Reagents

Potassium iodide crystals (KI)

6N hyrochloric acid CHCl)
O. 025N sodium thiosulfate, certified titrant (Na2SZ03)
Starch indicator solution
4.00 Safety
~n using glassware, reagents and
electrical equipment. For specific details, refer to the
appropriate MSDS or to a toxicity manual.

4.10 Use normal precautions

4.20 Always wear safety glasses and chemical gloves when handling

samples.

Handle all hot equipment with gloves or tongs.

5.00 Procedure

S.lO Weigh sample into a tared 250 mL Erlenmeyer flask.

weight to nearest 0.1 mg.
5.20 Add approximately 2 g KI.

Record

Add stir bar and stir until

crystals dissolve (about 1 minute) .

ALc%nz CORPORATXON
Qt1ALXTY ASSURANC3:

LABORATORY TEST METHOD

IN'DMDER: 1124
I

TZTLB:

DETERMINATION OF SODIUM CHLORITE:

50 PPM TO 1500 PPM CONCENTRATION

REY:Ol
2

I PAGE:

of

JEPP. DATE: ,SUPERSEDBS:

I

5.30 Add 1 mL of 6N Hel.

Stir for 30 seconds.

5.40 Continue stirring and titrate liberated iodine with

standardized o. 025N NaZSz03 (Sodium thiosulfate). When most
of the brownish iodine color has faded, add 2 mL starch
indicator solution and titrate to a clear endpoint. (Allow
adequate mixing time between additions of titrant near
endpoint.) Record volume titrated (V).
6.00 Calculation
(Y in mL Na&zPJl x 0.025 x 90.45 x 1000 - ppm NaC10z
sample size(g) x 4

where:

0.025 is the normality of the titrant

90.45 is the molecular weight of NaCIOz
1000 is conversion factor from mg/g to ppm
4 is milliequi valents NazSz~ per NaC10z

7.00 Suggested sample size

For
For
For
For

50-250 ppm, use 100 g sample

250-500 ppm, use 50 g sample
500-1100 ppm, use 20 g sample
1100-1500 ppm, use 15 g sample

ALCZDB CORPORATION
QUALITY ASSTlRANC3:

T:ITLE ~

LABORATORY TEST METHOD

DETERMINATION OF SODIUM CHLORITE:

50 PPM TO 1500 PPM CONCENTRATION
lAPPRO~BY:
1

m1
;

( d:fM

,NUMBER.:
, REV; O~
,PAGE: ~

I EFF. DATE:
I 09/13/95

1.1.24
of 2

st7PER.SBDBS :
OS/l7/95

1..00 Scope
Solutions containing sodium chlorite at 50-1500 ppm can be
assayed for sodium chlorite concentration by titration of the
iodine released in the presence of potassium iodide and
hyrochloric acid.
2.00 Equipment
250 mL Erlenmeyer flasks
Stir bars and magnetic stirrer
50 mL buret
Buret stand
Buret clamp
~oo mL graduated cylinders/ or balance capable of weighing 250g
3.00 Reagents

Potassium iodide crystals (KI)

6N hyrochloric acid (Hel)
o .025N sodium thiosulfate, certified titrant (Na2SZ03)
Starch indicator solution
4.00 Safety
4.~0

Use normal precautions ~n using glassware, reagents and

electrical equipment. For specific details, refer to the
appropriate MSDS or to a toxicity manual.

4.20 Always wear safety glasses and chemical gloves when handling
samples. Handle all hot equipment with gloves or tongs.
5.00 Procedure
5.~0

Weigh sample into a tared 250 mL Erlenmeyer flask.

weight to nearest o.~ mg.

Record

5.20 Add approximately 2 g KI. Add stir bar and stir until
crystals dissolve (about 1 minute) .

ALC%DZ CORPORATION
QUALITY ASStmAHC3:
TITLE:

LABORATORY TEST METHOD

INmmBR: 1124
, REV: 01
,PAGE: 2 of 2

DETERMINATION OF SODIUM ClnJORITE:

SO PPM TO l500 PPM CONCENTRATION

,BPP.
DATE: ,SUPERSEDBS:
,

5.30 Add 1 mL of 6N Hel.

5.40

Stir for 30 seconds.

Continue stirring and titrate liberated iodine with

standardized o. 025N Na2Sz03 (Sodium thiosulfate). When most
of the brownish iodine color has faded, add 2 mL starch
indicator solution and titrate to a clear endpoint. (Allow
adequate mixing time between additions of titrant near
endpoint.) Record volume titrated tV).

6.00 Calculation

(Y in mL

Na~~)

Q. Q~S

x 90.45 x 1000 -

sample size(g) x 4
where:

ppm NaCIOz

0.025 is the normality of the titrant

90.45 is the molecular weight of NaCIOz

1000 is conversion factor from mg/g to ppm

4

is milliequivalents

Na2S2~

per NaCIOz

7.00 Suggested sample size

For
For
For
For

50-250 ppm, use 100 g sample

250-500 ppm, use 50 g sample
500-1100 ppm, use 20 g sample
1100-1500 ppm, use l5 g sample