Answer guide for O Level Biology (5090/3) – Paper 3 Practical Test June 2001

1(a)(i)
mixture of S4 & S5
starch present ✓ ✓;
reducing sugars present ✗ ✓;
[must be 2 ticks / crosses per line] 2
(ii) By adding iodine solution / I in KI ; 1
(iii) By adding Benedict’s solution ;
heat in waterbath; 2
(iv)
water in beaker
starch present ✗;
reducing sugars present ✓;
2
Amylase digests / hydrolises /breaks down starch ;
into maltose / reducing sugar / glucose ; Not simple sugar
(maltose) molecule smaller than starch (molecule) ;
(passes through) membrane / visking / semi-permeable membrane ;
by diffusion ;
starch cannot pass through ; 5

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Answer guide for O Level Biology (5090/3) – Paper 3 Practical Test June 2001

(b)(i) Graph marks:

14

12

10

conc. of
reducing
sugar 6
(arbitrary
units)

0 2 4 6 8 10 12 14 16 18 20
time (min)

1. Size [50% of grid], clear & clean

2. x-axis labelled time / minutes
3. y-axis labelled reducing sugar concentration / arbitrary units
Accurately plotted
5. Adequate curve / ruled joined points, including 0 & 2 or best fit
[freehand or straight line]. 5
(ii) Starch not yet digested / needs longer to act ;
(reducing) sugar not yet diffused/etc ; 2
(iii) Curve levels off / flattens / decreased gradient ;
Until horizontal ; 2

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Answer guide for O Level Biology (5090/3) – Paper 3 Practical Test June 2001

2(a)(i) Drawing marks:

1. At least 6 cm. clear, clean, (realistic)
2. Cotyledons shown distinct from plumule / cutting with realistic
(leaf) attachment etc.
3. Root hairs reasonably well shown 3
Labels:
Root/radicle ;
Root hairs ; 2
Measurements of drawing (± 2 mm) & specimen – [mm or 0.1 cm]
units in both ;
Expressed length drawing over specimen length ;
Mag. correctly expressed ;
[Accept only 2 dp and rounding by 0.2 max] 3
(b)(i) Drawing marks:
1. TS of adequate quality, at least 5 cm across, showing vascular
bundles
2. Xylem / region differentiated 2
Labels:
Xylem / vessel ;
Vascular bundles / phloem ; 2
(ii) Ink / stain (travels where water travels) ;
in xylem ; 2
(iii) Root hairs absorb water ;
Absorb ions / salts / nitrates / minerals ;
[Stained areas] conduct / carry (water) ;
Support plant ; [up to 2] 2
(iv) Reference to transpiration ;
Loss of water from leaves / stomata ;
Continuous column (of water) from root to leaves ;
Suction force ;
Water pulled up ; [up to 3] 3

Requirements:

For question 1:
Length of Visking tubing
S4 = 5% starch solution
S5 = 2% amylase solution

For question 2:
S1 = cress seedling
S2 = stick of celery

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