228-30212

DIFFERENTIAL REFRACTIVE INDEX DETECTOR

RID-1 OA
FOR SHIMADZU HIGH PERFORMANCE

LIQUID CHROMATOGRAPH
SERVICE MANUAL

This manual is intended for the use of trained service engineers only. Unauthorized
personnel are advised not to attempt the procedures shown in this manual.

SHIMADZU CORPORATION
CHROMATOGRAPHIC & SPECTROPHOTOMETRIC
INSTRUMENTS DIVISION
KYOTO, J A D A N

Precautions for Safe Operation

The RID-1OA is the differential refractive index detector for the high performance liqid
chromatograph.
In order to operate the unit for safety, strictly observe the following points.
1.

Do not use the unit for the purpose other than the above mentioned analysis.

2.

Follow the procedures described in the instruction manual.

3.

Observe the warnings and cautions.

4. Do not disassemble or modify the unit without our approval. Failing to do so may lead
dangerous situation or damage of the unit.

NOTE
This instruction stipulates the content of warnings as follows:

1-

Applied for the case where death or serious injury is assumed

to happen at any moment to the repair engineer when a mistake
is made in the work or to the user due to the nonconformity of a
product occurring after the work.

1-

Applied for the case where the possibility of death or serious

injury is assumed of the repair engineer when a mistake is
made in the work or of the user due to the nonconformity of a
product occurring after the work.

1-

Applied for the case where there is a possibility of injury of

medium degree or slight injury to the repair engineer when a
mistake i s made in the work or to the user due to the
nonconformity of a product occurring after the work, or the
case where there is a possibility of machine damage.

1 -

Applied for information described for proper handling of the

unit.

For safety operation, this unit is provided with the

mark at the portion where special
cautions are required.
When operating the parts where this mark is indicated, exercise special caution after reading
the manual.

I WARNING I
Th~sunit uses the following fuse. Be sure to replace the fuse of same type and capacity.
Rated voltage : 100-24OV

Part NO. 072-01652-23 250V 5AT

Do not bend the optical cable for remote control with the radius of 35mm or less.

Precaution on installation site and handling- of the unit

Generally. a large amount of organic solvents is used in the high-performance liquid

chromatograph. Sufficient care should be taken in installation site and handling of the unit.
Please take care of the notices in the text, not to mention the precautionary requirements listed
below :
1.

Vent ilat ion

Solvents used in the high-performance liquid chromatograph are inflammable or toxic. Be
sure to ventilate the room well.

2.

Fire
Open flames are prohibited in the room where high-performance liquid chromatograph is
installed. Avoid installing units which emit sparks in the room.
It is also necessary to provide a fire extinguisher preparing for the worst.

3. Safety goggles
Wear safety goggles in handling solvents.
4.

Other equipment
A washstand or the like is necessary to be furnished nearby preparing for the cases such that
solvent comes into the personnel's eyes or the personnel touches toxic solvent.

5.

Power source
Working voltage range and power consumption of this system are as follows.
Be sure to connect to the power source conforming to them.
Part No.

Power source

Capacity

228-32000-91

IOOV

150VA

22 8- 32000-92

120v

150VA

228-32000-94

220-24OV

150VA

6.

Grounding
To prevent electric shock and to secure safe operation of the system, always make
grounding.

7.

Repair and maintenance of the unit

Normal maintenance of this unit can be performed without removing the cover. Do not
remove the cover at the normal maintenance. Contact our sales office or agent of repair
requires to remove the cover of the main body.

I
I

a.

9.

Solvent not usable

The following solvents affect the materials used in the wetted part and deteriorate the
strength of the material. In the worst case, pipes may burst and high-pressure solvent
scatters.
HFIPA (Hexafluoroisopropyl alcohol)
Others
The equipment should not be exposed to direct sunlight or strong air currents. It is also
recommended to install in a room where temperature fluctuation is small.

Precautions on Static Electricity

Liquid chromatography using flammable organic solvents as mobile phase requires proper care
against fire, explosion, etc. Particularly, among various possible accidents, those caused by static
electricity are difficult to anticipate, and tend to occur only with unexpected conditions which
often make countermeasures insufficient.
At a site where preparative liquid chromatography is practiced, a large amount of flammable
substances may be used. Therefore, once an accident happens, it could lead to tremendous
damage.
The mechanism of accident caused by static electrical discharge and preventive measures are
described below. Take due care in safety measures in handling of equipment.

I.

Mechanism of Static Electrical Discharge Accident (Example)

Accidents caused by static electricity take place through the following processes.

Electricity

When liquid is fed at high speed through a small-diameter

tube like the pipe of a liquid chromatograph, static electrical
charge occurs by friction between solid and liquid as shown in
Fig. 1.

Flowing liquid ___)

A : Electric charge
moving with
0 0 0@ @
flowing liquid
Q @@@0@@@@@8@
000000000000808
B : Elecn-ic charge
6
being fixed to the
A
-,/
A
solid surface.

0-

0-

Solid body

Fig. 1 Occurrence of Static Electricity by Friction between Solid and Liquid

Charging and storage
of static electricity

When the charged liquid is collected in an insulated vessel,

the static charge accumulates gradually, and the voltage can

easily reach a few kilovolts.

Energy release by

If some other conductive object is brought near the vessel,

electricity is discharged at a certain distance fiom the vessel
releasing heat energy.

substances

If flammable gas of sufficient concentration exists nearby,

ignition is caused by this energy.

If air dry

Spark discharge -

Liquid is flowing at high speed

through a smalldiameter tube.
*Charge is easily accumulated if
bubbles are included in the liquid.
Insulated vessel
(like polyethylene)
h?mri\

I @

l m

Inflammable gas is stored in the

head space of the vessel.

0!

e
-

Inflammable organic solvent is

charged to a great extent.

Floor covered with rubber, etc.,

provides unwanted insulation.

Fig. 2 Conditions for Accidents

2.

Preventive Measures against Accidents

The principal preventive measure is the prevention of charging and storage of static
electricity among those items shown in Occurrence mechanism of static electrical
discharge accidents. The preventive measures are shown below. It is recommended to
exercise two or more measures simultaneously.
+E Particularly when a large quantity of flammable solvent is held in a large vessel, be sure
to observe the preventive measures 1,2, and 3.

Preventive measure 1.
Use metallic (conductive) waste liquid vessel which is well grounded. This releases the
charge of the waste liquid and vessel to ground.
The following items are available.
(1) Grounding wire with clip
P/N 228-21 353-9 1
(2) Metallic 18 liter can
PIN 038-00044
(3) Metallic 4 liter can
P/N 038-00043-01
% Be sure to ground the vessel properly. Disconnecting of grounding wire or poor
grounding defeat the purpose of using a metallic vessel.
There are some metallic cans which have no conductivity due to an oxidized coating
or lacquer on their surface. Be sure to confirm the grounding of vessels by a tester
before application.
% When a liquid with almost no conductivity (of 10-%./mor less) is discharged into the
vessel, it is necessary to mix it with another liquid with some conductivity. (The other
liquid can be placed in the vessel in advance.)

M
_-

Preventive measure 2.
Minimize the clearance of both inlet and outlet of vessel to prevent flame from entering the
vessel.
(1) Cap with three holes for 18 liter and 4 liter cans (P/N 228-21354-91) is available.
Connect clip to metallic part
I

nnect to the grouning

erminal of the device to
ground the metallic vessel.

Minimize clearance
by attaching a cap.

Metallic 18 litter
(A plated can is

of the liquid
is released via vessel.

Fig. 3 Anti-Static Electricity Measures for Vessel

Preventive measure 3.
Do not approach the vessel with charged objects including the human body.
Charging prevention measures for human body
Prevention of charging of shoes and clothes
Grounding of human body
Make working floor conductive
Suitable products to be used for those measures a), b), and c) are available on the
market.
When persons who use no charge prevention measures approach dangerous sections,
they have to be grounded beforehand. (For example, they should contact grounded
metal by hand.)
Preventive measure 4.
Use pipes with inner diameter of 2mm or more for waste liquid line for large flow rates.
2 Inclusion of bubbles in the tube may increaqe the amount of charging by ten times.
Check that there is no inclusion of air via tube joints.

Preventive measure 5.
When it is impossible to use a conductive vessel, use caution in the following points.
a) Set the vessel so that the pipe outlet will be placed below the liquid level in the
vessel. Or, dip a grounded metal (ex. pipe connected to the main body of device) in
the liquid.
::: This method is not effective for liquid with small conductivity (lO-los/mor less).
b) Use a vessel of the smallest possible capacity to minimize the damage by fire if it
should occur.
c) Prevent the room from being dry. Humidity of 65% or more has charge prevention
effects.

CONTENTS

[ Chapter 1 1 jntroduction ...................................................................................

1 .1 Introduction ................................................................................................

I Chapter 2 1 Specifications ...............................................................................

1-1
1-2
2- 1
2-2

2.1

RID-] OA Specifications .............................................................................

3- I

3.1
3.2

Measuring Mechanism ................................................................

Principle of measurement ...........................................................................
Flow line diagram in the unit .....................................................................
Configuration and Functions ...................................................
Front Panel .................................................................................................
Interior of Front Cover, Right Side ............................................................

4-1

Chapter 4
4.1
4.2
4.3
4.4
4.5

...................................................................................................
Inside ..........................................................................................................
Inside of the optical unit .............................................................................
R e x Panel

List of Commands .......................................................................

Display ........................................................................................................

Chapter 5
5.1
5.2
5.3

6.1
6.2
6.3

Keyboard ....................................................................................................
AUX Functions ...........................................................................................

.........................................................................
Power supply voltage selection ..................................................................
Replacing the Fuse .....................................................................................
Electrical system connection diagram and circuit diagram ........................
Circuit diagram PB-1 ...........................................................................
PB-2, PB-3 ................................................................
PCB CPU ..................................................................

1 ChaDter 7
7. 1
7.2
7.3

7.4

7.5
7.6
7.7

Maintenance .................................................................................
Cleaning the Flow Lines .............................................................................
Span adjustment .........................................................................................
Replacing the ROM ....................................................................................
Replacing the Heater ..................................................................................
Replacing the Outer block heater ................................................................
Replacing the inner block heater ................................................................
Replacing the solenoid valve ......................................................................
Replacing the Lamp ....................................................................................
Disassembly of the optical system .............................................................
j

3-2
3-4

4-2
4-3
4-4

4-5
4-6
5-1
5 -2
5-3
5-5

6- 1
6-2
6-3

6-4
6-5
6-8
6-10
7- 1
7-2
7-3
7-7
7-8

7-8
7-1 1
7-13
7-16
7-19

8.1
8.2
8.3

Parts and Optional Units ...........................................................

Details of parts ...........................................................................................
parts Configuration

Optional Units

.....................................................................................

............................................................................................

Chapter 9 j Troubleshooting ..........................................................................

9.1 Troubleshootin_g ..........................................................................................
9.2 Failures indicated by error display .............................................................

8-1
8-2
8-3
8-4

9- 1
9-2
9-4

I 1
i

Chapter I Introduction

1.1

huoduction

.........................................................

............... ...............-..-.

1-2

1-1
I

RID-1OA

I
I

I.I

Introduction

--

Thls service manual describes maintenance of the IUD-IOA Differential

Refractive Index Detector for S h a d z u High Performance Liquid
Chromatograph. Description of the operating principle, unit configuration, and control circuits as well as how to perform the functions
and performance checks are summed up and edited so that it is easy
to use for a service engineer.
Although some items overlap with those of the instruction manual,
for installation and operation, read the instruction manual along
with this manual as required and perform adequate and effective
maintenance. Also, care should be taken to observe the precautions
for safety which is described in later chapter to avoid any accident.

c
0

Note

For improvement of the product, parts and circuits on this service

manual may be partly different from the ones actually used. and

also, we do not stock all the parts described on the disassembly

drawing and/or parts list as repair parts.

1-2

RID-1OA

Chapter 2 Specifications

2.1

ND-lOA Specificarions .........................................................................

2-2

2- 1

RID-1OA

2. 1

RID-1OA Specifications

RID-1OA Specifications
Measurine method

Deflection

Refractive index range (RIU)

1-1.75

I 0.01-500~10~"(Analysismode)

Measuring ranse (RKJ)

I - 5000~
I 0-6(Preparative mode)

j x 10-4(Analysis mode)

Linearity (RIU)

5xIO-'(Preparative mode)
Maximum operating flow rate (mlfmin) 20 (with option installed: 150)
0 . 2 5 ~ 1 0 -or
~ less (water seal, time constant3.&ec, room temperature

Baseline noise (RIU)

25T. analysis mode)

1x10.' or less (water seal, time constant 1.5sec., room temperature

Drift (RILih)

2 5 O C , analysis mode)

Detector 0.4 (4kgf/cm2)

Withstand pressure (MPa)

Cell
Detector internal capacity (p4

1.9 (20kgf/crn2)

Between inlet port and flow cell

: 63.5

Between flow cell and outlet port

: 280.2

Solenoid valve (pinch valve type)

Flow line switchover

Withstand pressure : 0.4MPa (4kgf/crn2)

Wetted part matenals

SUS316, PTFE,quartz, fluoro-rubber, liquid crystal polymer

Temperature setting for cell unit

30-6OoC (0.1 "C increments), OFF

Zero adjustment

Optical balance (optical zero)

Auto-zero, baseline shifting (Fine-zero)

Polanty switching

Possible

Time program

Stand-alone operation and control from system controller are both possible.

Set parameters : Auto-zero, range, marker, response, event, polarity,

Output output for recorder

1mV RECORDER terminal, lOmV RECORDER terminal

output for integrator

Selectable in 4 steps of 1 x

Safety measure

1 x 10

',1 x

2.5 x lO-"RTU/V

Bimetalic thermal breaker

Liquid leak sensor

Weight
Size W x H

D (mm)

Operating temperature range (OC)

12kg

I 260x 140x120

I 4-35

Power supply

RID- 10A is classified as follows according to supply voltage

PiN228-32000-91

lOOVklOV

150VA

50,60Hz

P!N228-32000-92

12OVklOV

150VA

50.60Hz

P!"228-32000-94

220VbOV

150VA

50.60Hz

2-2

RID-1OA

-b

Chapter 3 Measuring Mechanism

f

3.1
3.2

Principle of measurement .......................................................................

Flow line diaFam in h e unit .................................................................

3-2
3-4

3- 1

RID-1OA

3. 1

Principle of measurement
The following illustration shows the optical system of this unit.
The light radiated from the lamp passes through the lens slit and
passes through the cell in the form of parallel rays, which is, then,
reflected by the mirror and passes through the cell to finally form a
image of the slit on the photodiode.
The flow cell consists of the sample side and the reference side.
When the refractive index in the sample side cell varies, imaze of
the slit travels horizontally.

W lamp

en refractive index in
e flow cell varies,
f the slit moves.

This unit has a photodiode divided into four as shown in the figure
below. By selecting a combination of photodiode elements, the unit
can provide measurements from high-sensitivity analysis to high
concentration preparative analysis.

In mode A

In mode P and L

The photodiode is
regarded as two
elements, right and

The photodiode is
regarded as two
elements, upper and

Signal processing in mode A

A s i p a l is processed regarding each of the right and the left of the

photodiode as an individual element. Data processing is performed
regarding it as a two-division cell divided into right and left parts.
When the refractive index in the flowcell varies, balance of the
intensity of light into right and left cells changes. The change in the

-.

righdleft balance is converted into refractive index and then output.

_-

3-2

RID-1OA

3.1

RI

o=

Principle of measurement

A-B
TOTAL

Signal processing is performed regarding each of the upper and the

lower parts of the photodiode as an individual element. Since the

RI-

A-B
TOTAL

pq
A

Signal processing in mode L (when using the option)

Same as the mode P, signal processing is performed regarding each
of the upper and the lower parts of the diode as an individual cell.
However, since the reference side with less flow line resistance is
used as the measuring cell, when the refractive index varies, the
image moves in the reverse direction of that of mode P. Therefore
polarity is turned over before change in the balance is converted
into refractive index.

B-A

RI

cc:

TOTAL

3-3

RID-IOA

3. 2

Flow line diagram in the unit

The diagram shows flow lines in the unit.

When the flow selection block option is equipped, refer to 5.3.
At measurement
When R.FLOW switch is OFF, the solenoid valve is open at side A.
The liquid from the sample cell does not flow into the referenceside cell, but into the outlet port. Liquid inside the reference-side
cell is sealed in.

.-v,

At R.FLOW

When R.FLOW switch is Oh', the solenoid valve is open at side B.

The liquid from the sample flows into the outlet port after passing
through the reference-side cell. This is used to fill the sample cell
and the reference cell with the solvent of equal refractive index

0,

.-S

before starting measurement.

v)
(D

Outlet port

Inlet port

Piping capacity (At Rflow OFF)

Inlet port to flow cell

RID-1OA

Flow cell capacity

63.5
9

Flow cell to cell outlet port

280.2

,.

Chapter 4 Configuration and Functions

CONTENTS

4.1
~

4.2
4.3
4.4

4.5

Front Pane] .............................................................................................

Interior of Front Cover, Right Side ........................................................

4-2
4-3

..............................................................................................
Inside .....................................................................................................
Inside of the optical unit ........................................................................

4-4
4-5

Re= Panel

4-6

RID-1OA

4-1
I

4. I

Front Panel

Q
1

1 RID-1OA

v)

t
0

.c
0
S

& ON

, 0a O F F 0

LL

rn

0
.-c

m
L

No.

2
2

I
1

3-2

RID-1OA

Part name

Description

Control panel

Performs setting and display.

Front cover

Open this cover to connecVdisconnect tubing.

Front cover openinp button

Press this button to open the front cover.

4. 2

Interior of Front Cover, Right Side

I

\,

\,

RID-1OA

I I

Part name

No.

Connect the tube from column.

Cell outlet port

Connect the drain tube.

Tube holder

Fixes connected tube.

Connection pon for leak tube

Connect the leak tube.

Liquid leak sensor

Detects liquid leak and output an error signal.

*
-

$
G

Description

Cell inlet port

L7
.

4-3

RID-1OA

4. 3

Rear Panel

I
L

/
'I

No.

Description

7
i

REMOTE connector

Connector for SCL-10A.

External input and ourput terminals

Terminals for connecting external equipment.

,
?

Grounding terminal

Grounding is performed from this terminal.

Power supply inlet

Power cord is connected in here.

Fuse holder

Fuses are set in this holder.

L
.d

z
/i-

.2

Part name

4-4

RID-1OA

4. 4

Inside

I 4

4-5

RID-1OA

4. 5

Inside of the optical unit

4-

4 i
1

..

..
c

..-

Name

No.

G
S

s
P

&

4-6

PIN

PB-2 ASSY

228-3 1837-91

Light source block ASSY

228-32444-91

(W lamp ASSY)

228-32445-91

Linearactuator

228-33735

V glass ASSY

228-33732-91

Cell holder ASSY

228-32441 -9 1

(Cell)

228-32004

(Spacer cell)

228-32057

Slit holder ASSY

228-32443-91

~ t e r o g l a s ~ ~ ~ ~

228-33730-9 1

Back plate

228-32043

Chapter 5 List of Commands

v)

1
1

CONTENTS

5.2

Display ..................................................................................................
Keyboard ...............................................................................................

5-3

5.3

AUX Functions

.....................................................................................

5-5

5.1

5-1

RID-1OA

5. i

Display

Display unit

The display unit consists of display screen and indicator lamps.

Their descriptions and functions are as follows.

5 ! I No. 1

Display ordescription

0
I

Function
~

~ _ _ _ _

______

balance

Displays position of the light on photodiode.

RI (lo*)

Displays refractive index (unit : X lod RIU)

13

~ _ _ _

~ _ _ _

v)

E
0

1s

I
~

Tz

2 1

.-J
v)

mode

1
I

shift

remote

Time program operation indicator lamp

&

Lights when time program is being executed.

Remote mode indicator lamp
Blinks when controlled by SCL- 10A.

Displays measurement mode.

Shift key indicator lamp

prog run

Displays full scale of refractive index output to the recorder terminal (unit : X IO-bRILJ)

temp cont

Lights when the power is being supplied to the temperature controlled heater for optical
system unit.

Lights when the liquid is being supplied to the reference flow line side.

31

PO1 (-)

Reversed polarity output mode indicator lamp

5-2

RID-1OA

/yboard
19 keys on the fiont are used for operation and setting. They are

Keyboard

classified into the following three types.

(1)
STD-func keys
Pressing this key, specified operation is immediately execut-

(2)

shift-func keys
Pressing this key after pressing @ key, specified operation is executed. (17
key, etc.)

-1

(3)

1)

edit keys
Used for inputting parameters and editing a time program.
(ten-key, etc.)

STD-func keys
Auto zero key

1-(

Zero shift key

Pressing this key performs zero adjustment. Returns the baseline to

the zero position set by

.I-[

Zero position on the recorder can be moved by pressing this key. It

is moved upward by pressing 4 side and downward by pressing V
side.

2)

[mark] Mark key

Mark is added to the data being recorded in the recorder by pressing this key. Mark is not valid in the integrator output.

INn) Run key

It is a switch to start and stop a time program.

shift-func keys
@+@key

Moves to time program creation mode.

+ [balance] key

Drives the zero glass and adjusts its position to be optimal.

+ [=]

Switches the solenoid valve to replace the liquid inside the reference cell with the mobile phase.

key

5-3

_-

RID-1OA

5.2

Keyboard

+ (Po11key

Polarity of recorder output is switched by pressing h s key. [ pol ( - 1

LED is lit for (- ) polarity.

Selects measurement mode.

The display for measurement mode changes from A
A when this key is pressed.
A : Analytical mode
P : Preparative mode
L : Large-prep mode

(shlft) + @ key
(3)

+P +L 3

Moves to the response setting mode.

edit keys

io'l

-mNumeri

31 key

(Enter) Enter kev

Input value is set by pressing h s key.

(CE1Clear key

Returns the display screen to the initial state.

Input value is cleared by pressing this key when inputting a numerical value.
Display and alarm are cleared by pressing this key when error is
displayed. But, errors such as equipment failure cannot be cleared
with this key.

@+ Cdel)Delete key

Deletes a line in the program displayed in the time program.

(func! Function key

Advances to the next setting item in the display screen.

AUX. FUNC setting screen is forwarded.

+ (back)Back key

Numerical values are input with these keys.

Returns to the previous setting item by pressing this key. AUX.

FUNC setting screen is scrolled backward.

5-4

RID-1OA

'

X Functions

AUX. FUNC (AUXILIARY FUNCTIONS) available for this unit

is described.
The ones marked with * are maintenance commands. They are displayed by turning ON the power supply switch with the shift key
held down.
Function

Name

TOTAL EN

Indica,tes the total quantity of light irradiated into the light receiving element.

CELL

Displays the quantity of light irradiated into each divided element.

CELL TEMP

Sets the cell temperature at the measuring unit.

ACT TEMP

Displa.ys the current temperature at the measuring unit.

SETTEMP?,

Set the temperature of rhe temperature controller for the outer block. When not specified, it is set to

cell temperature minus 2C.

ACTTEMP2

Indicates the current temperature of outer block.

=A U X RANGE

Sets output range to integrator.

EVEhT

Sets external event output terminal status.

EXT-S

Used when event terminal is used for starting external equipment, etc.

MONn-TIME

Set when elapsed time is desired to be displayed in executing time program.

LAMP-TIME

Displays the lamp's lighting integration time.

CLOSE-KEY

Disables any key input.

Set address when controlled by SCL-10A.

LOCAL

* DIAG

1
1

LAMP VOLT

Selecrs control by SCL-10A or control by this equipment.

Self-diagnosis of the memory (ROM and RAM) is performed.

I
I

Sets the lamp voltage.

Displays zero glass position.
Displays leak sensor alarm level. The leak sensor operates at 50.000 or over.

SPAN

SPA!! P

~nputsthe span value of the analysis mode.

Inputs the span value of the preparative mode
the span value of large-prep mode (Valid when the flow selection block is used.)

-I

5-5
RID-1OA I

5.3 AUX Functions

The maintenance commands among AUX. FUNC list whose setting method is not stated in the instruction manual are described.

SET TEMP2
(Setting of the temperature of
temperature controller for the
outer block)

babnce

(Displays the current temperature of the outer block.)

mode

range

40.0

TEMP2

SET

Sets the temperature of temperature controller for the outer block.

If not set, it is set at (cell temperature minus 2C).
balance

ACT TEMP2

RI (10)

RI(10)

ACT

range

mode

38.0

TEMP2

Displays the current temperature of the outer block.

51
I
~

DlAG
(Self-diagnosis of the memory)

babnce

RI (10)

range

mode

D I AG
Self-diagnosis of the memory (ROM and RAM) is performed.
Note that the parameter is initialized in this occasion.

SIG MODE
(Change of the type in output signal)

bslsnce

RI (10)

range

mode

S I G MODE

Changes the type of the signal output. Usually set this to zero
0 : RI
1 : TOTALEN

2 : Right side photo cell

3 : Left side photo cell

ZG POSl
(Zero glass position)

mtance

ZG

RI11m

nmoe

mode

450

POSl

Displays the current position of the zero glass driving motor.

When a numerical value is set, the zero glass moves to the set position. (Input range: up to 800)
The home position of the motor is detected by the home position
sensor when power is turned on.

SENSOR
(Leak sensor output value)

balance

RI(10)

SENSOR

range

mode

38000

Displays the output value of the leak sensor. When this value
exceeds 50,000, the alarm sounds and ERR LEAK is displayed.
When the threshold value (50,000 or more) is input to this sensor

5-6

RID-1OA

value, the alarm level can be changed, although this setting is not
saved when turning the power off.

Chapter 6 Electrical System

6.1

6.2

6.3

..............................................................
Replacing the Fuse .................................................................................
Electrical system connection diagram and circuit dia,oram .....................
Circuit diagram PB-1 .....................................................................
PB-2, PB-3 ...........................................................
PCB CPU .............................................................
Power supply voltage selection

6-2

6-3
6-4

6-5
6-8
6-10

6- 1
7

RID-1OA I

6. 1

Power supply voltage selection

It is necessary with the RID-1OA to select the switch on the switching power supply ECX-OOSD (Section 4.4) when changing the
power supply voltage.
Be sure to disconnect the power supply plug socket before performing the following operations.
0 Switching regulator
Remove four M3 screws on the rear of the main unit
described in the following diagram to dismount the switching regulator.

6
E

Q,
c
v)
)r

-m

u)

.-0

Switkhing regulator

Remove the screws

-a,
0

r7

&

The switching regulator is described below. Mount a connector on the connector CP2 as required.
100 V system

------ Insert the connector.

200 V system ----.Remove the connector.

6-2

RID-1OA

6. 2

Replacing the Fuse

Replace the blown fuse as follows.

Be sure to use the fuse specified in the table below.

J,
,L
f--

2,

Voltagerange

Power

Fuse

AC100-240V

250VA

5A072-01652-23

Turn OFF the power supply switch

Disconnect the power supply cable from the power supply
connector.
Hook the slotted screwdriver to the fuse holder cover and
pull it out.

RI-

Slotted screwdriver

A
Fuse holder cover

1 Quantity

Is

After replacing the fuse, push it in until clicking sound is

heard.

'

Fuse holder cover

Fuse

6-3

RID-1OA

Electrical system connection diagram and

circuit diagram

6. 3

RID-1OA Electrical System

Connection Diagram

P
Battery

I PCB FIP

ASSY

T
PB-2A

1
I
al

sw

6 1

CNlO

v)

cn

6-4

'

(34.
CN6

Solenoid valve

Photocell

Outer temperature control

I

Q,

CN1 CN7
PB-1
CN5

0 CrJ I

Circuit diagram PB-1 RID-1OA

6-5-6

I I

'b

PB-1 RID-1OA

Electrical System

6.3 Electrical system connection diagram and circuit diagram

Photodiode

1 0-core flat,

R1 -R4:1 MF
c3-C6:O2O1 p F

> 228-23693

PB-2

6 1

PB-3

20K RM2lOK

R7 iOK

CN2

al

CI

v)

>
v)

PREP MD

ANAL MD
To PB-1

vcc
Vee
0.47
f

!L+5v

C)

AMPAD708

--

)---

CPU-90

Circuit diagram PB-21PB-3 RID-1OA

6-8

RID-1OA

2
0.47

$10

6.3 Electrical system connection diagram and circuit diagram

I
C')

-r*2

PB-2 RID-1OA

jI

f=

-R.

PB-3 RID-1OA

6-9

RID-1OA

Circuit diagram PCB CPU (112)

6-11-1 2

t5v

-5v

CN6

t
10rnVF.S.

1mV F.S.

ov
ov
AUX1 O.SAU/V

/Z
A D 7 0 8 M 7 2/Z

CN 3

CJ

M15

I
-12v

LT1019
M6

Circuit diagram PCB CPU (212)

0- I 3- 1 4

6.3

Electrical system connection diagram and circuit diagram

6-15

RID-1OA

_-

Chapter 7 Maintenance

CONTENTS

7.1

7.2
7.3
7.4

7.5
7.6
7.7

7-1

RID-1OA

7. 1

Cleaning the Flow Lines

Cleaning the Flow Lines

Dirty cells and pipings cause the phenomena such as unstable base
line and large base line noise due to pulsation of liquid delivery
pump. In this case, clean the flow lines with the following procedures.

I
I
I
I

Item tobe prepared

Q'ty

Syringe

Adapter

Distilled water

1oOmlI

Acetone
0. I N nitric acid

pGFG$

Mixing nitric acid with organic solvents may form explosive

substance. Be fully careful for handling the waste liquid.
Mount the adapter on the syringe and supply acetone from
the inlet port to clean the flow lines. Press (ShlftandlRflow]
to clean the reference-side flow lines as well.
(2) Supply distilled water and clean the flow lines with the similar procedure.
( 3 ) Next, clean with 0.1 N nitric acid.
(4) Rinse off the dirt and the nimc acid with the distilled water
sufficiently.
( 5 ) Purge the flowline with the mobile phase.
When the mobile phase used for analysis is not soluble with
water, replace the flow lines with acetone and return to the
mobile phase.
(1)

7-2

..
.

7. 2

Ii Span adjustment
Inject the standard solution whose refractive index is known, and
adjust the detector output so that the size of the signal becomes the
regulated height.
Span adjustment should be performed in each mode of A/P/L.
Following is the description about mode A span adjustment. When
the Chromatopac is used, refer to p.7-3, and when the LC workstation is used, refer to p.7-5.

*)

items to prepare

Q'ty

Chromatopac or CLASS-LC 10 LC workstation

Syringe

Adapter

Pure water (distilled wateri'de-ionized water)

1OOml

Refractive index standard sample*

1 OOml

Refractive index standard sample : glycerin solution 0.872 $1.

How to make the solution : Resolve 43.6 g of glycerin (USP) in 11 of
water saturated with air, and dilute this solution 50 times to make refractive index standard solution. (Reference document : ASTM E1303-89)
This sample shows difference of 100 x
RIU refractive index from
that of the pure water.
% USP

United States Pharmacopeia

% ASTM

American Society for Testing and Materials

When span adjustment is performed using the Chromatopac

Turn ON the main unit POWER switch.
(1)
(2) Set the parameter of the detector as follows.

I Set value

Item
LOCAL

1 (*I

CELL TEMP

40

Polarity

I maie
*)

Remarks

I When the ambient temperature is 28OC or below

I

When S

adiustment
~
at analvsis mode is oerformed

When the RID-IOA is controlled from the SCL-IOA system controller

: This adjustment is performed on the detector side. First, input
LOCAL

1 to disconnect control (link) from the SCL. After that,

input each set value by the numerical keys of the detector main unit.

.-

7-3

RID-1OA

7.2 Span adjustment

Set the detector so that the plot full scale of the Chromatopac
becomes 128 x
MU.
AUXRANGE of the detector AlTEN of the Chrornatopac
2

Supply distilled water from the inlet port using the syringe
and the adapter.
Turn Rflow on* during this to supply the water also to the
reference side flow lines.
(*) Press [Shift)[Rflow) , and [Rflow]lamp is lit.
After the sample celvreference cell inside is replaced with
distilled water, press IShlft')key and [Rflow] key again to supply liquid to the sample side again.
Press
key and (Rflow] key to perform optical balance
adjustment.
When the baseline is stabilized, press the Plot key following
the (zerol key, and record the baseline level with distilled
water inside the cell.
Inject standard sample from the inlet port. Check that the
baseline is deflected to 80% of the full scale. Record the
baseline level with standard sample inside the cell.
Read the difference between the baseline of distilled water
and that of the standard sample, and convert the value into
RI unit.
Example) When the base line moves 120 mm on the chart paper
with the Chromatopac C-R7A, the difference value can
be converted into RI unit with the of following formula.

Q,

Q,

c,

.-

Full scale of the Chromatopac [RILTlx

= 128 x lO"[RIU] x
*)

Actual nusuremnt value of deflection width on the chart [m]

Full scale on the chart [mm]

120 [mm]
150 [mm]

=102.4 x 10"WIU]

Plot full scale of the Chromatopac is as follows.

~~

Chromatopac model

Full scale on chart paper

C-R7A

150mm

C-R4A

160mm

C-R5A/R6A

135mm

~~

7-4

RID-1OA

Span adjustment

7.2

(1 0) Span coefficient is obtained with the followin,0 formula.

Span coefficient after calibration =

100 [lORrU]
Span coefficient before dibrauon
Actual
measurement
d u e of the refracuve mdex [RIE]
(S PAN-A 1
Example) When the measured refractive index is 102.4 x
[NU]
and the old span coefficient (SPAN-A) is 0.90, the new
span coefficient after calibration is :
0.90 x 1001102.4 = 0.88 t SPAN-A after calibration
(1 1) Input the span coefficient obtained at (10) into SPAN-A.
(12) Inject distilled water from the inlet port to end span adjustment.

(13) For span adjustment of mode Pimode L, change the mode

setting in (2) to P or L, and follow the procedures from (4).
Input the span value obtained in each mode into SPAN-P
and SPAN-L respectively.
When span adjustment is performed using the CLASS
(1) Turn ON the power supply of the RID main unit and CLASS
system (PCICBM).
(2) Set the parameter of the CLASS-LC10 as follows.

1 Analysis screen 1

rolaritr

@i+!
..... 0 -

Measure Mode

@ O:Analytical

01:Preparative
0...,;.. . ,... .
;
,

;:f: :
;

-Response

v u

A U RANGE
01:1.OE-7
@ 2:l.OE-6
031.OE-5
04:2.5E-7

RIUlmV
RIUlmV
RIUlmV
RIUlmV

Set A?TEN so that the vertical axis of the chromatogram of the

analysis screen becomes more than 100 pRlU.
Example) When AUXRANGE setting is 2, set ATTEN = 7 .
(3)

7-5

RID-1OA

7.2

Span adjustment

Supply distilled water from the inlet port using the syringe
and adapter. Click on R.FLOW(f8) during supply and
replace the reference flow line with distilled water.
After the sample celvreference cell inside is replaced with
distilled water, press R.CLOSE (f8)to supply the liquid to
the sample side.
Perform
Wait until the base line is

.1-

stabilized.
After the baseline is stabilized, press

following

- [Zero compensation], and record the baseline

level with distilled water inside the cell.
Inject standard sample from the inlet port. Check that the
baseline is moved about 1OOpIUU to plus direction.
When the base line is stabilized, move the cursor to read the
difference between levels of the distilled water and standard
sample.
Obtain span coefficient with the following formula.
Span coefficient after calibration =
100 [IOdRIU]
Span coefficient before calibration

(SPM-A)

Actual measurement value of the refractive index [Rnr]

Example) When the measured refractive index is 102.4 x l o 6

[RIU] and the old span coefficient (SPAN-A) is 0.90,
the new span coefficient is after calibration is :
0.90 x loo/ 102.4 = 0.88 t SPAN-A after calibration

.-

s"

After disconnecting link between the RID-1OA and the

CLASS, input the span value into the detector main unit.

ifUnk)

key on the RID-IOA several times

Press
to display LOCAL. Input 1 into LOCAL to disconnect it from CLASS control.
Input the value obtained in (10) into SPAN-A.
Example) SPAK-A 0.88
After inputting. input 0 into LOCAL again to
return it to CLASS control.
Inject distilled water into the inlet port to end span adjustment.
As for the adjustment of mode P and mode L, change the
measuring mode setting item to preparative/lar,e-prep in (2),
and perform procedures from (4). Input the span value
obtained in each mode into SPAN-PISPAN-L respectively.

7-6

RID-1OA

7. 3

Replacing the ROM

Replacing the ROM

Replacement of the ROM at the occurrence of abnormality with the

ROM on the printed board CPU 90 Assy (ROM FAILURE), and at

_-

the time of upgrade of ROM version is described.

Be sure to disconnect the power plug socket before performing the
following operation.
Remove two M3 screws on the rear and one M4 screw to
dismount the printed board CPU90 ASSY.

Dismount the printed board CPU 90 Assy and replace the

M23 ROM on the printed board.

RID-1OA
~~

7. 4

Replacing the Heater

Replacement of the outer block heater

Replacement of the outer block heater is described.
Be sure to disconnect the power plug socket before performing the
following operation.
A

.l-,

Remove the following connectors on the printed boards

PB-1 CN2 (ZG HP)

CN3 (ZG motor)
CN6 (Outer temperature control)
CN7 (Inner temperature control)
CN9 (Lamp)
PB-3A CN2
CN2

CN6 (Outer temperature control)

control)

CN2

*-

-4
A

2,

Remove two M3 screws and dismount the top plate

M3 screws

Top plate

RID-1OA

-CN9

(LAMP)

7.4

,&

4,-

Disconnect piping Sin 2,

Sout

Replacing the Heater

s,Rin 2,and Rout g.

Remove four M3 screws and one M4 screw and dismount

the front side plate.

M4 Screw

I====
Front side plate

Remove three M4 optical unit fixing screws on the bottom

surface of the main unit chassis.

M4screw

7-9

RID-1OA

7.4

Replacing the Heater

Ji.

/?

Being fully careful not to harm the wiring ZG HP and wiring

ZG MOTOR, dismount the optical unit with heat insulator.
Remove the heat insulator after it is taken out.

Remove the heater Assy mounted on the bottom surface of the

optical unit (outer block). Mount a new heater Assy (22833738-92). inserting the insulation sheet between the transistor and the outer block.

Heater Assy (228-3373892)

ws/
r-

0I
I

I
I

0I
1

Bottom surface of the optical unit (outer block)

7-10

RID-1OA

7.4

Replacing the inner block heater

,-

il/

J,

Replacing the Heater

Follow the procedures (6) Removal of the optical unit in

"Replacing the outer block".
Remove four M4 screws to dismount the cover heat block.
Remove five M 4 screws mounted on the pipe holding plate
to dismount the plate. Remove four M4 screws of the front
plate heat block to dismount the block.
Heat block cover

/----7

P p e holding plate
M4 screws

M4 screws

Front plate heat block

Remove three M3 screws fixing the inner block, from the

outer block bottom surface.
Q,

Bottom surface of the optical unit (outer block)

7-1 1

RID-1OA

7.4

Replacing the Heater

,--,

.&

Take out the inner block

Inner block

Remove the heater Assy mounted on the bottom surface of

the inner block. Mount a new heater Assy (228-33738-91),
inserting the insulation plate (060-49779-04) between the
transistor and the inner block.

a,
0

m
c

a,

.-

r"

7-12

RID-1OA

._

..

1
1

7. 5

Replacing the solenoid valve

Replacing the solenoid valve

It is necessary to replace the solenoid valve when liquid leaks due

to excessive back pressure applied to the valve, Refer to the fol-

lowing procedures and replace the valve.

Be sure to disconnect the power supply before performing the following operation.

I-

Bands marked with number are attached to piping (SUS pipe

or Teflon tube), which enables identification of piping. The
numbers attached to the piping and flow lines are listed
shown below. Refer to these numbers when replacing the
solenoid valve.
Modes A a n d P
Flow cell

Sin

i 7
S w a y block

1
Coupling

Solenoid valve

OUT

IN

Modes A and P (using flow selection block)

Flow selection block

IN

7-13

RID-1OA

7.5

Replacing the solenoid valve

Mode L (flow selection block)

w
Solenoid valve

Flow selection block

lri I I
/ I l-2

OUT

/-.
2
A

:$

7-14

RID-1OA

t
I IN

Refemng to the flow lines and piping numbers inside the

unit described above, loose the male nut PEEK of the piping
Nos. 3,@, and 3,to remove the piping.
Remove the connector CN5 (S.V) of printed board PB-1.
Remove two screws shown in the figure below and dismount
the solenoid valve (with Teflon tube).

7.5

Replacing the solenoid valve

..

Using a small plier, remove the Teflon tubes from the dismounted solenoid valve with care, and then, observing the
piping No. shown in the following figure, mount the tube
tightening it by fingers until it hits the replaced solenoid
valve.

No -0
Solenoid valve

a
3

Re-tighten the finger-tightened Teflon tube 30Omore by a

1
I7
I
Q,

small plier.
Fix the solenoid valve by screw with the COM facing upward
as shown in the above figure. Then, refemng to
piping
inside the unit, perform the connection of piping.

I=
E

tu

Q,

7-15

RID-1OA

1 7 . 6

Replacing the Lamp

Replacing the Lamp

Time of lamp replacement

The service life of a lamp is 20,000 hours. When the lamp
1)
for a long period exceeding its service life, the filament is
blown and the analysis may not be performed. When the
lamp lighting time (LAMP TIME) exceeds 20,000 hours,
replace the lamp.
If TOTAL EN is 5,000 mV or less despite lamp voltage
2)
(LAMP VOLT) adjustment, replace the lamp. However,
other possible causes for low quantity of light are bubbles
inside the flow cell and dirty flow cell. Be sure to perform
*7.1 Cleaning flow lines to check that the low quantity of
light attributes to the lamp itself.
m

&
P

J,

7 l

Remove the main unit power supply cable and the cover.
Remove two M3 screws on the top plate to dismount the
plate and heat insulator.
Then, remove four M4 screws of the heat block cover and
dismount the heat block cover and the heat insulator below.
M3 screws

Heat insulator

&%z

I -

I!

Heat block cover

Heat Insulator

7-16

mRID-1OA

_,

7.6 Replacing the Lamp

Remove four M4 screws on the base cover to dismount the

base cover.

M4

base cover

Remove connector CN9 on printed board PB-1.

Loosen two M3 fixing screws on the light source block in
the following diagram with a wrench to dismount the W
lamp Assy.

tight source block fixing screw

4%

Light source block

Insert connector CN6 of new W lamp Assy into the PB-1.

Next, insert the lamp to the end of light source block.

W lamp Assy (228-32445-91)

7-17

RID-1OA

7.6

Replacing the Lamp

Insert the power supply cable into the main unit and input
LAMP VOL = 5.00 to light the lamp.
Rotate the lamp so that the image of the filament lamp
becomes horizontal on the cell holder and fix it with M3
hexa,oon screws.
Image of lamp filament

Cell holder
,'--

&

Assemble the unit and adjust the quantity of light by LAMP

VOL.

7-18

RID-1OA

7. 7
Replacing the Cell

Disassembly of the optical system

When re-assembling of the cell is required due to liquid leakage from

the cell unit, refer to the following procedures to replace the cell.
f-J, Follow the procedures of until Removal of inner block in
7.4 Replacing the inner block heater.
P
& Remove four M4 screws fixing the heat block cover to dismount the block.
n
& Remove two M4 screws fixing the pipe holder R in the following figure and dismount the pipe holder R.
@I

.-

Pipe holder R---

Remove four M4 screws fixing R cell joint pipe.

The R pipe is bent by jig, so be careful not to bend the pipe.

Remove the screws of R cell joint and dismount the R cell

joint, cell spacer, cell, and cell spacer.

cell

k cell joint

Cell spacer

7-19

RID-1OA

7.7

Disassembly of the optical system

&

Clean or replace the cell, and then, being careful to the direction of the cell, mount the cell spacer, cell, cell spacer, and R
cell joint in this order. When fixing the R cell joint with
screws, tighten the upper and lower screws equally by the
tightening torque of 3 kof.cm2 so that the clearance with the
cell holder becomes even.

m
e

J,

lo

01

Fix the R cell joint pipe with screws. When fixing, attention
should be paid not to apply force to the directions shown in
the following figure and tighten the screws.

7 1

&

Disassembly of the optical system 1.

2.

Tighten the screw so that force is not applied to the arrowhead directions.
Assemble and fix the pipe holder R with M4 screws.

Removal of the outer block

For the removal of the outer block, refer to steps
to @ in
7.4 Replacing the outer block heater and perform replacement.
Removal of the inner block
To take out the inner block from the outer block, refer to
to @ in 7.4 Replacing the inner block heater.
steps 3

7-20

RID-1OA

Chapter 8 Parts and Optional Units

8.1

8.2
8.3

Details of parts .......................................................................................

parts Configuration ................................................................................
Optional Units ........................................................................................

8-2
8-3
8-4

j -8.
tn
c.
.r

L
Q

8- 1

RID-1OA

8. 1

1
I

Details of Parts

Parts list
PartsNo.

228-32445-91

Part name

Remark

W lamp

Repair parts
Remark

Part name

Electrical system parts

228-33009-98

Panel. RID- 10A

1-23673-02

Panel switch. RID

1228-33968-91
228-23527-91

Power switch
Printed board PCB-CPU90

228-33806-91

ROM RID-1OA (CPU90)

~~~

1 228-3 1627-91 1
I 228-31837-91 1

Printed board PB-2

228-32578-91

Pnnted board PB-3

228-33743-91

Leak sensor

228-25249-9 1

Battery

228-33738-91

Heater Assy (for inside block)

228-33738-92

Heater Assy (for outside block)

I
I

Pnnted board PB- 1

~~

Optical system pans

.-S
v)

228-32444-91

Optical source block

228-32443-91

Slit holder

228-33748-9 1

Photodiode

228-33730-91

H zero glass

228-33732-91

V zero glass

3
I0

228-32442-91

Mirror holder (with mirror)

.-0

228- I5706

Mirror

228-34210-91

Zero glass motor

228-33736-91

Micro switch (for motor)

r.

CI

Piping pansiflow line pans

228-341 14-91

Solenoid valve

228-33816-01

Tube (for solenoid valve, inlet side)

Tube (for solenoid valve. outlet side)

228-33816-02
I

228-32004

I 228-16447 1
I 228-16447-01 I
I 228- 16034

n o w cell

Inlet port
Outlet port
Kut (for mounting the port)

228- 16033

Washer (for mounting the port)

228-32017-91

S cell side flow line (with heat exchanger)

228-32018-9 1

R cell side flow line

I
I
I

I
~~~

8-2

RID-1OA

-92 IOOV systrni (1:)

-93 200v systcln (19

RII)-lOA ASSY

- Optical unit R I D

228-320()1-9I
228-33739-9I

I k a t block, hcat insulator, ctc.

Innrr block ASSY

228-33734-9I

Limit switch ASSY

228-33736-9I

Grounding c;ible ASSY

228-2 1752-92

llcater ASSY

228-33738-9 I

Optical bench ASSY

228-33733-91

Slit holder ASSY

228-32443-9 1

S cell joint ASSY

228-32017-91

Photoccll ASSY

228-33748-9I

R ccll joint ASSY

228-32018-9I

V zero glass ASSY

228-33732-9I
V zcro glass
sub ASSY
228-3244 1-9I

228-32445-91

Mirror ASSY

228-32442-9 I

228-33730-9 I

Stand, etc.

- Solenoid vnlvc ASSY

228-338 14-9I

Inlet angle ASSY

228-337 19-91

Solenoid 1,PYA ctc.

W lamp ASSY

H zero glass ASSY

- PB-3A ASSY
.-

228-~37.~92

Outcr block ASSY

I lcat insulator, ctc.

1le;rter ASSY

Controller RID

228-32578-91

Base, flow cell etc.

228-32002

PCB CPU RID- IOA

2 2 8 - 2 4 9 8 9 - 9 5 1 PCR CPU 90 ASSY

PB- I ,RID- I 0

228-3 1627-9I

Switch ASSY

228-32344-9 I

Pancl ASSY

228-23009-98

Leak sensor ASSY

228-33743-9 I

Battery, SPD

228-25249-9 I

Cable ASSY

228-33834-91

Cover, plate and hcnt insulator

surrorrntling the optical unit etc.

Acccssory ASSY, RID 228-33744-9 I

Instruction manual
RID -1OA

I-

228-30179 (E)

Parts and Optional Units

Fl

ROM RID- IOA

228-23527-9 I
228-33806-9 I

1 lB2 ASSY R I I ) 228-3 1837-0I

Photodiotlc, etc.
228-3373 1-9

'

8. 3

'

Optional Unit

Relief valve ASSY

When excessive back pressure (more than 3kgf:'cm') is applied to

PIN228-23615-91

the detector, the relief valve releases pressure to prevent dama,oe on

the solenoid valve and flow cell.
Configuration and external vieu

J,

Housing ASSY

I 2 I
I 3 1
I & 1

8 ,
c
rn
.-

PtN

Name

NO.

228-32068-9 1

Diaphragm

I
I
1

Holderspring

HousingB ASSY
Plunger

228-32070
228-32071
228-32072
-034-01020-02

&,

Slit holder ASSY

228-32443-9 1

Holder, relief valve

228-32074

I
I
I

Flow selection block

This is an optional unit which selects the flow lines inside the

P/N22&34102-91

detector and enables liquid to be supplied in a large flow rate, up to

150mOmin. (Without this optional parts, the flow rate is mar;.
20mNmin.) Use this unit for large-prep. amount mode (L) and parallel flow mode.
Configuration and external view

U
S
tu

l!4

Sectional drawing

Sectional drawing

8-4

RID-1OA

-1

8.3 Optional Units

Mounting the flow selection block

Mounting the optional flow selection block enables selection of the

measurement mode which comprises with each usage of high-sensitivity analysis, semi-preparative, and large-prep.
(With the standard specifications, only the high-sensitivity mode
and semi-preparative mode are available.)

I Note

Mounting of the flow cell block option should be performed by a

service engineer.

Measurement mode Mode setting

Application
Analytical mode
(A)
For general analysis
The same flow lines with the
RID- 1OA without the flow selection block are provided.
(This is the compatible mode
with the RID-6A.).
Semi-preparativemode (P)
This mode is used for measuring samples of high concentration.
It is possible to measure samples that are ten times as concentrated as those for mode A.
Large-prep mode
(L)
This mode is used for measuring samples of high concentration at large flow rate. It is possible to supply liquid at 150

Usage of each mode

Measurement mode
Analysis

Symbol
A

'

Refractive index measuring range

(x

Semi-prparative
Large-preparative

lo* RIU)

- 500

Flow rate range

(mlmin.)
20

-so00

20

-5Ooo

150

cn

-c:
0

Piping drawing

CI

8
rn

,?

ii/

il/

E
Q

2,

EQ

r\

The way of piping for analysis mode/semi-preparative mode

8-5

RID-1OA

8.3

Optional Units

2,

Piping for large-prep mode

Drain
bottle

I Caution

About the drain tube

The following drain tubes should be used for large-prep mode.
The longer and the thinner drain tubes than specified ones may
curse back pressure exceeding the pressure resistance specifications to be applied to the detector.

FEm::y !

Note

When extending the tube

Drain tube

Outer diameter [mm] xlnner diameter [mm] x Length [cm] Outer diameter [mm] x Inner diameter [mm] x Length [cm]

50or less

1.6 x0.8 x 100

3.2 x 1.6 x 100

150 or less

3.2 x 1.6 x 100

3.2 x 1.6 x 100

When using the fraction collector

Perform the following piping when using the fraction collector.
When connecting the FRC- 1OA
Flow rate
[mhin]

F R C l O A inlet tube
F R C l O A outlet tube
Outer diameter [mm] dnner diameter [mm] x Length [cm] Outer diameter [mm] x Inner diameter [mm] x Length [cm]
1.6 x 0.8 x 100 (FRCstandard)

50 or less

I 150 or less

3.2 x 1.6 x 100

3.2 x 1.6 x 100 (FRC standard)

3.2 x 1.6 x 100 (FRC standard)

When using a fraction collector of other manufacturers

Check that connecting the fraction collector does not apply back
pressure exceeding the pressure resistance of the detector. It is necessary to take into the consideration the pressure changed at preparative valve selection.

8-6

RID-1OA

Chapter 9 Troubleshooting

9.1

9.2

Troubleshooting ......................................................................................
Failures indicated by error display .........................................................

9-2
9-4

...

9- 1

RID-1OA

9. 1

Troubleshooting

9.1 .ICauses of the troubles with liquid and flow lines

Phenomena of the drift, noise, and fluctuation on the baseline are
listed on the below table.
In addition, the following cares should be taken as general.
0
When the unit is operated under incorrect conditions of carrier

liquid, temperature, and pH, the fixed phase of the column filling map be flown out. This may cause noise.
When solubility of the sample substance to the carrier liquid is

smaller than that to the sample solvent, the sample may be separated out. This may cause noise increase.
When the column is dirty with the sample substance due to

repeated sample introduction, noise and drift may be caused.

Other common causes of drift and noise are slight leak of liquid
from the connection portions and generation of the bubbles
caused due to insufficient degassing. This kind of troubles can
be avoided through sufficient inspection and solution.

Troubleshooting
~

I - I

Drift

1. Large temperature change

~~~

1. Operate the unit at a location of small tempera-

ture change. Turn ON the temperature adjustment switch.

2 . Dirty mobile phase (carrier liquid)

2. Replace it with clean mobile phase.

3. Dirty f l o ~
cell

3. Clean the flow cell.

4. Liquid leak from the flow lines

4. Repair the leaking portion.

5 . Varied flow rate of the mobile phase

5 . Stabilize the flow rate.

I
1

I
6. Dirty flou lines

II
i'

(Refer to the instruction manual of the liquid

delivery pump.)

6. Clean the column, sample injector, pump, and

the piping.

7 . Bubbles in the flow cell

7 . Eliminate bubbles inside the flow cell.

8. Liquid leak inside the cell unit.

8. Re-assemble the cell.

9. Cloggin,o of the flow lines

9. Repair the clogged portion.

10. Troubles of the control unit

10. Replace the print board.

i
j

1 !. Fixed phase inside the column filling is flown

1 1. Replace the column or the mobile phase.

i
~

out.
12.

Insufficient replacement of the solvent inside

(Refer to instruction manual for column.)

12. Repeat R. FLOW operation.

the solenoid valve

13. Liquid leak from the solenoid valve

13. Repair the faulty portion, or replace the sole-

noid valve.
14. Insufficient replacement inside R cell

14. Replace the liquid sufficiently by R.FLOW

15. Varied amount of air solved inside the solvent

15. Change to the stabilized degassing method.

1
9-2

mRID-1OA

9.1 Troubleshooting

-~

~~

Remedy

Noise

1. Impurit) such as dirt inside the cell.

I . Clean the flow cell.

2 . Bubbles inside the floa cell.

2. Eliminate the bubbles inside the flow cell.

(Degass the mobile phase and eliminate bub-

i
'

bles inside the flow lines.)

3. Liquid leak from the flow lines.

3. Repair the leaking ponion.

4. Breakdown of electric circuit.

4. Repair the faulty portion.

5. Faulty light source lamp.

5. Replace the lamp.

6. Large influence of the pulsation on the baseline.

6. Mount the pulsation absorption unit.

Mount a resistance tube right behind the pump
to increase the pump pressure.

Eliminate bubbles inside the flow lines.

7 . Unstable flow rate of the mobile phase.

7. Eliminate bubbles inside the liquid delivery pump.

8. Faulty operation of the solenoid valve.

8. Replace the solenoid valve.

Clean the solenoid valve; supply liquid into the
solenoid valve to eliminate dirt.

9.1.2 ALARM display

When the quantity of light, that is TOTAL EM, becomes 5,000

rn or less or when it is beyond 9,000 mV, ALARM is displayed.
The possible causes of h s problem are listed as follows. Check
and give solutions on them.
Cause
1. The cell is dirty.

2. Mobile phase used for the cell

R chamber is different from

that is used for S chamber.

1. Clean the cell. (Refer to 7.1 in

this manual.)
2. Replace the solution inside the

R chamber.

3. Bubbles inside the cell

3. Drive out bubbles.

4. The lamp is not adjusted and

4. Check that the lamp is fixed cor-

fixed correctly.
5. The light source lamp is not
being lit.

6. The quantity of light exceeds

9.000 mV

rectly. (Refer to this manual.)

5. Check the lamp control. Replace
the lamp.

6. Lamp control adjustment.

9-3

RID-1OA

9. 2

Failures indicated by error display

Failures indicated by error display

R0.M error

1 ) Replace the M23 @OM) or M23 (RAM)

! 1 ) Abnormality has occurred with

(ROM FAILLRE)

on the printed board CPU 90 Ass!.

the ROM or RAM on the printed

board CPU 90 Assy.

RAhl error
~

(RAM FAILURE)

2 ) Replace the faulty parts.

2 ) Abnormality has occurred with

(Pnnted board CPU 90 Assy.)

an element or circuit on the

printed board CPU 90 Assy.
Set value erase error

(SOTPROTECTED)

1
i

1) Check the battery voltage (with the sup-

1 ) Back-up voltage for rnemon stor-

plied voltage of 2.7 V or larger) or check

age is too low or not supplied.

the connecting portion on the printed

board CPU 90 Assy.
2 ) Replace the faulty parts.

2 ) Abnormality has occurred with

(Printed board CPU 90 Assy)

an element or circuit on the

printed board CPU 90 Assy.

Perform setting again in the case of 3) and

3) Power is cut-off while the data is

edited. Or power supply is shut-

4).

off right after transformation of

electricity.
4) Abnormal noise has generated

due to lightening and things like

that.
5 ) Be sure to turn ON/OFF the power sup-

5 ) Noise has generated due to turn-

ing ON/OFF the power supply

ply by the power switch.

by connecting/disconnectingthe
power plug.

Failures indicated by error display

Phenomena

Zero glass home position error

(ERR ZG HOME)

I
~

Possible causes
I ) Motor or home position sensor

does not operate correctly.

2 ) Connector is disconnected.
Leak sensor error

(ERR OVER HEAT)

RID-1OA

1 ) Replace the faulty part.

2 ) Check the connection on the printed board

PB-1.

3 ) Replace the printed board PB-I Assy.

1) Liquid leak

1 ) Eliminate the cause of the leak and wipe

1 ) When the temperature of the

1) Replace the heater Assy.

out the liquid on the leak sensor.

measuring point rises to 63C

and over.

9-4

Remedy

3) Fault); element on the printed board.

(ERROR LEAK)
Overheat error