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Aptamer Modified Graphene-Based Catalytic Micromotors - Off On Fluorescent Detection of Ricin PDF
Aptamer Modified Graphene-Based Catalytic Micromotors - Off On Fluorescent Detection of Ricin PDF
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Letter
pubs.acs.org/acssensors
DOI: 10.1021/acssensors.5b00300
ACS Sens. 2016, 1, 217221
Letter
ACS Sensors
DOI: 10.1021/acssensors.5b00300
ACS Sens. 2016, 1, 217221
Letter
ACS Sensors
resulting in a loss of the dye uorescence, which is recovered
upon the specic binding with the target ricin.
As shown in Figure 2, an excess of both nontarget proteins
(100 g/mL vs 10 g/mL ricin) displays a negligible
uorescence recovery (Figure 2C,D,G), compared to the
strong recovery observed in the presence of the target ricin B
(Figure 2B and G(a)). Similarly, the uorescent signal is
recovered fully upon adding the ricin toxin to solutions
containing an excess of BSA and saporin (Figure 2E and F,
respectively, and bars in G), demonstrating the high specicity
of the surface-bound aptamer toward the ricin B toxin. To
prove the important role of the self-propelled micromotors and
their self-mixing capability in the dramatically accelerated of
ricin detection, the dependence between the uorescence
recovery (due to the toxin binding) and the speed of the
motors (in the presence of dierent fuel levels) was tested
(Figure 3). No uorescence quenching eect was observed at
the dierent H2O2 fuel concentrations used (SI Figure S3).
DOI: 10.1021/acssensors.5b00300
ACS Sens. 2016, 1, 217221
Letter
ACS Sensors
ASSOCIATED CONTENT
S Supporting Information
*
AUTHOR INFORMATION
Corresponding Author
*E-mail: josephwang@ucsd.edu.
Author Contributions
ACKNOWLEDGMENTS
This work was supported by the Defense Threat Reduction
Agency (grants nos. HDTRA1-13-1-0002 and HDTRA1-14-10064). D. F. Baez and A. Jodra acknowledge fellowships from
CONICYT-CHILE and the Spanish Ministry of Economy and
Competitiveness, respectively. The authors thank A da Mart n
for her advice.
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