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Odour Formation On Textiles - Fresenius
Odour Formation On Textiles - Fresenius
Odour Formation On Textiles - Fresenius
Malodour?
Caroline Amberg
Swissatest testmaterials ag, St. Gallen, Schweiz
February2014
Introduction
CarolineAmberg
Over10yearsatSwissatest testmateirals ag:laundryhygiene,
microbiologicaltesting(disinfectants,products,food),applied
R&D(laundryhygiene,biofilmformationinhousehold
devicesandwatersupplysystems,Odourformationon
textiles)
Swissatest Testmaterials ag
February2014
Spinoffcompanyoftheswiss federalinstituteofmaterial
testingandresearch
FormerEMPATestmaterials ag
Development,productionandsalesoftestmaterials for
detergentandwashingmachineproducersandtextile
industry+Microbiologicallab
Knownproblemonsyntheticfibers (sweatodour)
Washingatlowtemperaturewithaliquiddetergentisnot
sufficienttoremoveallmalodourcomponents
Malodouraccumulationoldsweatmaldourontextiles
Dailylifecomfortisreduced
Lifecycleofthetextileisdecreased
Highercostsanduseofressources
February2014
Malodour ontextiles:Microorganisms
Microbial growth:
a) Microbial growth on the fiber: Bacteria form
a biofilm on the fiber. They use sweat and
sebum compounds (odorless) as nutrients,
and degrade them to odorous volatiles
c)
Chemical adsorption:
b) Odorous substances produced by the skin
flora are adsorbed to the fiber,
and released over time
A combination of these effects
February2014
Malodours
pluswater
Dirtylaundry:wetconditions,contactwithother
textiles,malodours
Washingprocess:
removalofcertainmalodourcompounds
Interactionofdetergentandsoftenerwithfiber(dye,
finishingetc)microorganismsandvolatilesubstances
Wear:Axillasweatandbodywarmth
Interactionwithmicroorganismonthetextile
Interactionwithvolatilesubstancesalreadypresentonthe
textile
Interactionwithfiber,dye,finishingetc.
February2014
February2014
February2014
Biotransformationinthe axilla
Steroids
Corynebacteria
Odorous
androstene
Sources: James, Casey, Hyliands and MyCock, 2004; Austin and Ellis, 2003
February2014
10
February2014
Organism
Gram
Odoroussubstancesproduced
SelectedReferences
Bacillus licheniformis
Bacillus subtilis
isobutyric acid,
isovaleric acid,
2methylbutyricacid
Ara etal.2006
Corynebacterium xerosis
5androst2en17one
Obendorfetal.2007,
Dumasetal.2009
Corynebacterium
tuberculostearicum
Corynebacterium bovis
3methyl2hexenoicacid,
3methyl3hydroxyhexanoicacid
Natschetal.2005
Corynebacterium jeikeium
3methyl2hexenoicacid,
3methyl3hydroxyhexanoicacid,
3methyl3sulfanylhexan1ol
Natschetal.2005,
Bratt&Dayan2011
Corynebacterium minutissimum
Corynebacterium striatum
5androst2en17one,
3methyl2hexenoicacid,
3methyl3hydroxyhexanoicacid,
3methyl3sulfanylhexan1ol
Obendorfetal.2007,
Natschetal.2003,
Natschetal.2005,
Bratt&Dayan2011
Propionibacterium acnes
propanoic acid,
aceticacid
Jamesetal.2004
Staphylococcus epidermidis
isovalericacid,
5androst2en17one
Obendorfetal.2007,
Munketal.2001,
Araetal.2006,
Dumasetal.2009,
Troccazetal.2004,
Goweretal.1997
Staphylococcus haemolyticus
3methyl3sulfanylhexan1ol
Troccaz etal.2004
Troccazetal.2004
Troccazetal.2004
Troccaz etal.2004
11
Corynebacterium sp.
3methylobutanoicacid
(Isovaleric acid)
3hydroxy3methylhexanoicacid
February2014
12
February2014
13
Source:McQueenetal.2007,TextileResearchJournal
February2014
14
15
16
TotalCorynebacterium sp
TotalCorynebacterium Subgroup
Wool
cotton
polyester
Source:McQueenetal.2007,TextileResearchJournal
February2014
17
Bacterial attachment and growth ontextilesis probably notthe only reason for
malodour formation and persistance
Source:ElizabethA.Grice&JuliaA.Segre,NatureReviewsMicrobiology9,244253(April2011)
February2014
18
Chemicaladsorption
DifferentFibers have differentbinding /retention
properties for sweat malodour compounds:
Isovaleric acid
19
Amount of microorganisms /
Corynebacterium is notdirectly related to
the maldour intensity of the textile
Relationbetweenmalodourontextiles,microbialinteractionand
chemicaladsorptionisnotfullyunderstandandsubjectoffurther
investigation
February2014
20
February2014
21
3methyl2hexenoicacid
pluswater
Isovaleric acid
Depending onthe cycle:
Temperature
Detergent (pH)
Water /Mechanical action
Powder detergent
(pH10to 11):
Mostcarboxylic acid are
removed by the washing
process
Androstene??
Liquiddetergent
(pH89):
Volatilefatty acids are
nottotally removed
5androst2en17one
22
Literaturereference
1hexen3one(Ketone)
Munketal.(2000)
1octen3one(ketone)
Munketal.(2000)
Ethyl2methylpropanoate(ester)
Munketal.(2000)
Ethylbutanoate(ester)
Munketal.(2000)
4heptenal(aldehyde)
Munketal.(2000)
Octanal (aldehyde)
Munk etal.(2000)
2octenal(aldehyd)
Munketal.(2000)
ChungandSeok (2012)
Methional(Aldehyd)
Munk etal.(2000)
2nonenal(Aldehyd)
Munk etal.(2000)
2,6nonadienal(Aldehyd)
Munk etal.(2000)
2,4nonadienal(Aldehyd)
Munk etal.(2000)
2,4decadienal(Aldehyd)
Munk etal.(2000)
Hexanal(aldehyde)
ChungandSeok (2012)
Nonanal(aldehyde)
ChungandSeok(2012)
Decanal(aldehyde)
Takeuchietal(2012)
4methoxybenzaldehyd(aldehyde)
Munketal.(2000)
3methylbutanoicacid(fattyacid)
Takeuchietal.(2012)
4methyl3hexenoicacid(fattyacid)
Kimotaetal.(2012)
Takeuchietal.(2012)
5methyl4hexenoicacid(fattyacid)
Takeuchietal.(2012)
6heptenoicacid(fattyacid)
Takeuchietal.(2012)
4methyloctanoicacid(fattyacid)
Munketal.(2001)
4ethyloctanoicacid(fattyacid)
Munketal.(2001)
Guaiacol
Munk etal.(2001)
Volatilefatty acids
January2014
aldehyds
ketones
23
water consumption
washing temperature
Dubai,January2014
24
Summary
Malodour formation is aresult of microbial transformation of former odourless sweat
and sebum compounds
Malodour intensity correlates with the number of aerobesand number of
Corynebacterium sp.
Onthe textilemalodour sources are more complex:
Microbial activity onthe textileitself
Chemicaladsorption of the volatilesubstances produced by bacteria onthe skin
and onthe textile
Chemicaladsorption seems to be important and driven by the fiber properties like
hydrophobicity
Thetypical sweat odour compound (volatilefatty acids)are removed inawashing test
with apowder detergent (highpH)whereas aliquiddetergent low temperature cycle
is notable to remove them totally
Afterwashing,the textileshave differentmalodour profiles then before washing:
Aldehydes and ketones are more prominentafterwashing
Alow temperature cycle with aliquiddetergent is notable to remove bacteria on
textilecompletely.Furthergrowth afterwashing is probable.
R. Donlan, (2002).
Biofilms: Microbial Life
on Surfaces.
February2014
25
Odour projects
CTIprojectNr.13310.1PFFLINM:Developmentof new textilecoatings reducing
bacterial adhesion finished 2013
Cooperation of Swissfederal laboratories for materialtesting and research (EMPA),SANITIZEDAGand
Swissatest Testmaterialsag
February2014
26
Thankyouforyourattention
February2014
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