Index

A
About command 133
Accuracy
Definition 247
Accuracy of measurements 249
Acquire
Command 14
Macro call 141
Acquire image
Command 71
Activate
Macro call 141
Add images 29, 87
Add Noise filter 88
Add to Image command 90
AddAnnotation
Macro call 142
AddAnnotationsToImage
Macro call 144
AddNoise
Macro call 144
AddNote
Macro call 144
AddOverlayToImage
Macro call 145
AddTextAnnotation
Macro call 145
AdjustBrightness
Macro call 146
AdjustContrast
Macro call 147
AdjustHue
Macro call 147
AdjustSaturation
Macro call 148
Again command 74
Alignment of data numbers 56
AND operation 94
AndOverlay
Macro call 148

Angle measurement
In degrees 105
In radians 106
Annotate image
Example 138
Annotation Mode command 122
Annotation toolbar 74
Annotation Tools command 124
Annotations 44
Arrows 46, 124
Bring to Front 125
Copy 125
Cut 125
Delete 125
Ellipses 46, 124
Erasing 46
Filling 47
Freehand 45, 124
Highlight 124
Line 124
Lines 46
Lock 125
Note 124
Paste 125
Polygon 124
Polylines 124
Properties 125
Rectangles 46, 124
Select 124
Select All 125
Send to Back 125
Text 45, 125
Undo 125
Annotations command 76
ApplyLookupTable
Macro call 149
ApplyOverlayGrid
Macro call 150
Area
Defined 251

Index

267

Area measurement 41
Command 106
Defined 251
Example 114, 136, 137
Measurement tab 104
Arrange Icons command 131
Arrow drawing 46
Aspect ratio
Defined 251
Average
Macro call 150
Average Blue measurement 106
Average filter 88
Average Green measurement 106
Average images 29, 30, 87
Average intensity
Defined 252
Average Intensity measurement 106
Average Red measurement 106
Axis
Defined 252

B
Background color
Annotations 47
Setting 16
BackgroundBlue
Macro call 151
BackgroundGreen
Macro call 151
BackgroundRed
Macro call 152
BAS file
Defined 252
Basic Language Help command 132
Binary filter See Overlay Filter
Binary image
Defined 252
BitsPerPixel
Macro call 152
Blue 81, 84, 143, 151
Add noise filter 88
Adding noise to an image 144
Color separation 84, 211
Custom LUT 26
Custom LUT values 150
Hex code 237
Intensity measurement 107
Setting background color 151
Setting weighting value 152
Spiral LUT 26
BlueWeighting

268

Index

Macro call 152

BMP file 12
Defined 253
Boundary filter 96
Brightness command 84

C
C++ constants 234
Calibrate Distance and Area
command 79
Calibrate Intensity command 78
CalibrateIntensity
Macro call 153
CalibrateIntensityFromWorksheet
Macro call 153
CalibrateScaling
Macro call 154
CalibrateTranslation
Macro call 154
CalibrateXYThreePoints
Macro call 155
Calibration 154, 156, 229, 244
Applied to multiple images 128
Example 139
Intensity 78, 79, 154, 229, 244
Saving 19
Spatial 80, 139, 154, 156, 229
Cascade command 131
Center of Mass Binary X
measurement 107
Center of Mass Binary Y
measurement 107
Center of Mass Gray X measurement
107
Center of Mass Gray Y measurement
107
Center of mass measurement 41, 138
Defined 253
Change Resolution command 82
ChangeColorResolution
Macro call 156
Classify data
Example 138
Classify Objects
command 100
Macro call 157
Clear
Macro call 158
Clear command 73
ClearAllOverlays
Macro call 159
Clearfield equalization

Pseudo-clearfield 27
Clearfield Equalization 28, 159, 167
Command 80
Example 136
Purpose 27
ClearFieldEqualization
Macro call 159
Click
Defined 254
Clipboard
Defined 254
Close All command 132
Close command 69
CloseActiveImage
Macro call 160
CM See Center of Mass
CollectMeasurement
Macro call 160
Color distortion problem 16
Color Threshold command 86
Color thresholding 161
ColorThreshold
Macro call 161
Column Statistics 49, 5760
Combine
Macro call 162
CombineOverlays
Macro call 162
Command
About 133
Acquire 71
Add to Image 90
Again 74
Annotations 76
Arrange Icons 131
Basic language help 132
Brightness 84
Calibrate Distance and Area 79
Calibrate Intensity 78
Cascade 131
Change Resolution 82
Classify Objects 100
Clear 73
Clearfield Equalization 80
Close 69
Close All 132
Color Threshold 86
Compare 87
Contrast 85
Convert to Gray Scale 81
Copy 72
Copy Calibrations 80
Count Objects 103
Crop 91

Cursor Shape 128

Cursor/Pen Size 127
Customize Toolbar 126
Cut 72
Delete Cells 99
Duplicate 88
End Macro 98
Exit 72
Filter-Custom 90
Filter-Standard 88
Find 73
Flip along horizontal axis 91
Flip along vertical axis 91
Format Cells 99
Gamma Correct 83
Generate Pseudo-Clearfield 81
Go To 99
Graph 75
Halftone 83
Help Topics 132
Hide All Overlays 78
Histogram 85
Histogram Stretch 85
Hue 83
Image Math 87
Inset Cells 99
Intensity Threshold 86
Invert 81
Keyboard Pointer 126
Lookup Tables 82
Macro editor help 132
Macros 127
Magnifying Glass 126
Mask 96
Measure Objects 103
New 69
New Macro 97
Normal View 76
Open 69
Options 128
Overlay 1 77
Overlay 2 77
Overlay 3 77
Overlay 4 77
Overlay 5 77
Overlay Filters 95
Overlay math 94
Page Setup 71
Paste 73
Pause Macro 98
Pause Recording Macro 98
Print 71
Print Preview 71
Properties 97

Index

269

Replace 74
Resample 91
Resize 92
Restore 91
Rotate 180 degrees 92
Rotate 270 degrees 93
Rotate 90 degrees 92
Rotate any angle 93
Run SigmaPlot 127
Run SigmaStat 127
Saturation 84
Save 69
Save All 70
Save As 70
Search for help on 132
Select Source 71
Separate 84
Settings 104
Shift Columns Left 104
Shift Columns Right 104
Show All Overlays 78
Snap window to Image 96
Sort Selection 99
Splice 93
Standardize Palette 83
Start Recording Macro 98
Start/Resume 97
Status Bar 75
Stop Recording Macro 98
Tile 131
Tip of the day 133
Toolbars 74
Track Edges 101
Track Lines 102
Undo 72
Window Leveling 85
Zoom In 76
Zoom Out 76
Commands
How manual refers to commands 6
Common questions 241
Compactness measurement 41, 108,
235
Defined 255
Compare
Macro call 164
Compare command 87
Connectivity of objects
Defined 255
Contrast command 85
Convert to Gray Scale command 81
ConvertToGrayScale
Macro call 164
Convolution filter See Gray filter

270

Index

Copy
Command 72
Macro call 165
Copy Calibrations command 80
CopyCalibrations
Macro call 165
CopyOverlay
Macro call 166
CopySelectedAnnotations
Macro call 166
Count objects
Command 103
Example 137
CountObjects
Macro call 166
CreatePseudoClearfield
Macro call 167
Crop
Command 91
Example 136
Macro call 167
Crossing value
Defined 256
Cumulative Distance measurement
108
Cursor Pen Size command 127
Cursor Shape command 128
Cursor size problem 243
CustomFilter
Macro call 168
Customize Toolbar command 126
Cut command 72
Cut, Copy and Clear commands 15
CutMacro call 169
CutSelectedAnnotations
Macro call 169

D
Data See also Worksheet
Cell borders and colors 57
Classify 100
Clearing worksheet 50
Cut, copy and clear 52
Deleting cells 54
Fonts 55
Graphing 60
Inserting cells 54
Number alignment 56
Number format 51
Opening data files 59
Paste 53
Printing 59

Saving 58
Sorting 99
Worksheet organization 50
Deactivating measurements 172
DefaultBPP
Macro call 170
DefaultHeight
Macro call 170
DefaultWidth
Macro call 170
Delete command 99
Delete Edge Objects filter 96
Delete Residues filter 96
Densitometry 244
Despeckle
Filter 88
Macro call 171
Dilation filter 96. See Overlay,
Dilate
Distance 41, 139
Cumulative 108
Measurement 108
Setting calibration units 230
Setting units 213
Dithering
Macro call 171
Divide images 29, 87
DoNotCollectMeasurement
Macro call 172
Drawing on the image See Annotate
image
DrawLineOnOverlay
Macro call 172
DrawMajMinAxes
Macro call 173
DrawObjNumbers
Macro call 173
DrawOnOverlay
Macro call 173
Duplicate
Macro call 174
Duplicate command 14, 88

E
Edge speed 178
Defined 257
Edge tracking 227
Description 1013
Example 136
Macro call 224
Edge Tracking 243
EliminateObject

Macro call 174

EliminateObjectAt
Macro call 174
Ellipse, drawing 124
Emboss
Macro call 175
Emboss filter 88
End Macro command 98
EndMeasurement
Macro call 175
Erosion filter 95. See Overlay, Erode
Excel
Working directly with 245
Exit command 72

F
Factor value
Defined 257
FastPaint
Macro call 176
FavorBlack
Macro call 176
Feret diameter 41, 108
Feret Diameter measurement 108
File type
Image 12, 70, 206
Worksheet 59
Fill
Auto Threshold 119
Macro call 176
Manual Threshold 119
Fill holes
Example 138
Fill Measurement Mode command
121
Filter, grayscale 89
Add noise 88
Average 88
Despeckle 88
Emboss 88
Gradient 88
Laplace 89
Line segment detection 89
Median 89
Mosaic 89
Oilify 89
Posterize 89
Prewitt 89
Sharpen 89
Shift and Difference 89
Smoothing 88
Sobel 90

Index

271

Filter-Custom command 90
FilterOverlay
Macro call 178
Filters, grayscale 88
Filter-Standard command 88
Find command 73
Flip Along Horizontal Axis
command 91
Flip Along Vertical Axis command
91
FlipHorizontal
Macro call 179
FlipVertical
Macro call 179
Fonts
Setting worksheet fonts 55
Format Cells command 99
Frame grabber
Defined 258

G
Gamma Correct command 83
GammaCorrect
Macro call 179
Gel analysis 244
Generate Pseudo-Clearfield
command 81
GetCalibratedIntensity
Macro call 180
GetCell
Macro call 180
GetCellText
Macro call 181
GetCellValue
Macro call 181
GetColumnName
Macro call 181
GetExeFileDirectory
Macro call 181
GetImage
Macro call 182
GetNumberOfObjectAt
Macro call 182
GetOverlayPixel
Macro call 183
GetPixelIntensity
Macro call 183
GetWorksheet
Macro call 183
Go To command 99
Gradient filter 88
Graph 129

272

Index

Column assignments 130

Command 75
Creating 60
Graph title 130
Grid lines 130
Plot type 130
Print 62
Regression lines 130
Symbols or Lines 130
Window 4
X axis label 131
X axis logarithmic 131
Y axis label 131
Y axis logarithmic 131
Gray filters See Filters, grayscale
Gray level
Defined 258
Green 81
Add noise filter 88
Adding noise to an image 144
Color separation 84, 211
Custom LUT 26
Custom LUT values 150
Hex code 237
Intensity measurement 108
Setting background color 151
Setting weighting value 184
Spiral LUT 26
GreenWeighting
Macro call 184
Grid
Drawing on overlay 68, 150
GridSampling
Macro call 184

H
Halftone
Macro call 185
Halftone command 83
Height
Macro call 185
Help
On-line 8
Help Topics command 132
Hide All Overlays command 78
HideAllOverlays
Macro call 185
HideAnnotations
Macro call 186
HideGraph
Macro call 186
HideOverlay

Macro call 186

High color (16-bit) mode 241
Histogram 2122, 65
Intensity
Defined 259
Histogram command 85
Histogram stretch 22
Histogram Stretch command 85
Hue 41, 65, 115
Adjustment command 147
Color separation 84, 211
Color thresholding 161
Command 83
Measurement 109

I
Image
10, 12, and 16 bit 64
Closing 13
Convert to gray scale 20
Crop 17
Defined 259
Duplicate 14
Filters 30
Histogram 21
Histogram stretch 22
Math 29
Measurements 41
New 13
Open 12
Print 20
Properties 20
Restore 18
Save 19
Splice 17
Image Math command 87
Image processing toolbar 74
Insert Cells command 99
Intensity measurement 109
Intensity resolution 249
Intensity Threshold command 86
IntensityThreshold
Macro call 187
Invert
Macro call 187
Invert command 81
IsValidCell
Macro call 188

J
JPEG file 12

K
Kernel values
Defined 259
Keyboard Pointer command 126

L
LabelingOverlay
Macro call 188
Laplace filter 89
Layer See Overlay
Lighting
Uneven 167
Compensating with clearfield 27
Compensating with pseudoclearfield 27
Example macro 136
Macro call 159
Line Intensity measurement 109
Defined 260
Line Segment Detection filter 89
Line tracking 102, 120, 121, 184,
205, 22427
Line X measurement 109
Line Y measurement 109
LineIntenWidth
Macro call 188
Load image See Image, Open
Lookup table 23, 2426, 82, 149
Custom 26
Defined 260
Example 136
Monochrome 24
Rectangular - monochrome 24
Rectangular color 25
Sawtooth - color 26
Sawtooth - monochrome 25
Spiral 26
Lookup Tables command 82
LUT 23, 2426, 82. See Lookup
Table

M
Mac file 12
Macro
Language 3, 4, 141
Macro call
Acquire 141
Activate 141
AddAnnotation 142
AddAnnotationsToImage 144

Index

273

AddNoise 144
AddNote 144
AddOverlayToImage 145
AddTextAnnotation 145
AdjustBrightness 146
AdjustContrast 147
AdjustHue 147
AdjustSaturation 148
AndOverlay 148
ApplyLookupTable 149
ApplyOverlayGrid 150
Average 150
BackgroundBlue 151
BackgroundGreen 151
BackgroundRed 152
BitsPerPixel 152
BlueWeighting 152
CalibrateIntensity 153
CalibrateIntensityFromWorksheet
153
CalibrateScaling 154
CalibrateTranslation 154
CalibrateXYThreePoints 155
ChangeColorResolution 156
ClassifyObjects 157
Clear 158
ClearAllOverlays 159
ClearFieldEqualization 159
CloseActiveImage 160
CollectMeasurement 160
ColorThreshold 161
Combine 162
CombineOverlays 162
Compare 164
ConvertToGrayScale 164
Copy 165
CopyCalibrations 165
CopyOverlay 166
CopySelectedAnnotations 166
CountObjects 166
CreatePseudoClearfield 167
Crop 167
CustomFilter 168
Cut 169
CutSelectedAnnotations 169
DefaultBPP 170
DefaultHeight 170
DefaultWidth 170
DeleteAllAnnotations 171
Despeckle 171
Dithering 171
DoNotCollectMeasurement 172
DrawLineOnOverlay 172
DrawMajMinAxes 173

274

Index

DrawObjNumbers 173
DrawOnOverlay 173
Duplicate 174
EliminateObject 174
EliminateObjectAt 174
Emboss 175
EndMeasurement 175
FastPaint 176
FavorBlack 176
Fill 176
FilterOverlay 178
FlipHorizontal 179
FlipVertical 179
GammaCorrect 179
GetCalibratedIntensity 180
GetCell 180
GetCellText 181
GetCellValue 181
GetColumnName 181
GetExeFileDirectory 181
GetImage 182
GetNumberOfObjectAt 182
GetOverlayPixel 183
GetPixelIntensity 183
GetWorksheet 183
GreenWeighting 184
GridSampling 184
Halftone 185
Height 185
HideAllOverlays 185
HideAnnotations 186
HideGraph 186
HideOverlay 186
IntensityThreshold 187
Invert 187
IsValidCell 188
LabelingOverlay 188
LineIntenWidth 188
MajMinLabelOverlay 189
MakePermanent 189
MarginBottom 190
MarginLeft 190
MarginRight 190
MarginTop 191
Mask 191
MeasurementBackspace 191
MeasurementLeftClick 192
MeasurementRightClick 192
MeasureObjects 193
MedianFilter 193
Mosaic 194
NewImage 194
NewWorksheet 194
NotOverlay 194

Oilify 195
OpenImage 195
OpenImageEx 196
OpenWorksheet 196
OrOverlays 197
Paste 197
PasteToAnnotations 198
PasteToNewImage 198
PlotGridLines 198
PlotLines 199
PlotRegression 199
PlotSymbols 199
PlotXLogarithmic 200
PlotYLogarithmic 200
PlotYVsRow 200
Posterize 201
Print 201
PrintGraph 201
PutCell 202
RedrawPlot 202
RedWeighting 203
RefreshColumnLabels 203
Resample 203
Resize 204
RestoreImage 204
Rotate 204
SamplingFrequency 205
Save 205
SaveAll 206
SaveAs 206
SaveHistogram 210
Select 210
SelectAllAnnotations 210
SelectTwainSource 211
Separate 211
SetAreaUnits 212
SetCellText 212
SetCellValue 212
SetCursorWidth 213
SetDistanceUnits 213
SetGraphLabel 214
SetGraphXColumn 214
SetgraphYColumn 214
SetIntensityUnits 215
SetOverlayPixel 216
SetPixelIntensity 216
SetRoundCursor 216
SetSquareCursor 217
SetXAxisLabel 217
SetYAxisLabel 217
SetZoomLevel 218
Sharpen 218
ShiftColumnAssignmentsLeft 218

ShiftColumnAssignmentsRight
219
Show 219
ShowAllOverlays 220
SnapWhenResize 220
SourceOverlay 220
SpatialFilter 221
Splice 222
StandardizePalette 223
StretchHistogram 223
TrackEdge 224
TrackingBlackOnWhite 224
TrackingGridOriginX 225
TrackinggridOriginY 225
TrackingGridSpacingX 226
TrackingGridSpacingY 226
TrackingSpeed 227
TrackingStability 227
TwainNative 228
TwainShowStatus 228
TwainUI 229
UncalibrateIntensity 229
UncalibrateXY 229
Undo 230
UnselectAllAnnotations 230
ViewAnnotations 231
ViewGraph 231
ViewOverlay 231
Width 232
XOrOverlays 232
XYContinuous 232
Macro Editor Help command 132
Macro example
#1 Automatically measure objects
135
#2 Clearfield equalization 136
#3 Edge tracking 136
#4 Shape analysis 137
#5 Summing coins 137
#6 Finding circular objects 138
#7 Splicing images 138
#8 Calling Word 97 from
SigmaScan Pro 139
Macro toolbar 75
Macros 47, 63, 64
Macros command 127
Macros examples 135
Magnifying Glass command 126
MajMinLabelOverlay
Macro call 189
Major axis 11011, 118, 137
Defined 261
Major Axis End Point measurement
110

Index

275

Major Axis Length measurement 110

Major Axis Slope measurement 111
MakePermanent
Macro call 189
Manual layout 5
MarginBottom
Macro call 190
MarginLeft
Macro call 190
MarginRight
Macro call 190
MarginTop
Macro call 191
Mask
Macro call 191
Mask command 96
Maximal perimeter 114
Measurement 111
Measure Objects command 103
Measurement
Angle in Degrees 105
Angle in Radians 106
Annotation Mode 122
Annotation Tools 124
Area 41, 104
Command 106
Example 114, 136, 137
Average blue 106
Average green 106
Average Intensity 106
Average Red 106
Blue 41
Blue Intensity 107
Center of mass 41, 138
Center of Mass-Binary X 107
Center of Mass-Binary Y 107
Center of Mass-Gray X 107
Center of Mass-Gray Y 107
Compactness 41, 108, 235
Cumulative distance 108
Distance 108
Feret diameter 41, 108
Fill Measurement Mode 121
Green 41
Hue 41, 109
Intensity 109
Line Intensity 109
Line X 109
Line Y 109
Major axis 11011
Major Axis End Point 110
Major Axis Length 110
Major axis slope 111
Minor Axis End Point 111

276

Index

Minor Axis Length 112

Minor Axis Slope 112
Number of objects 41, 113, 166
Number of Pixels 113
Overlay Draw Mode 123
Overlay Grid Mode 123
Perimeter 113
Perimeter - Cumulative 113
Perimeter-Diagonal 114
Perimeter-Horizontal 114
Perimeter-Maximal 111
Perimeter-Vertical 114
Red 41
Red intensity 115
Saturation value 115
Selection Mode 121
Shape factor 41, 115, 13738
Slope 41, 115, 235
Sum of X 115
Sum of X Squared 116
Sum of XY 116
Sum of Y 117
Sum of Y Squared 117
Tally 41, 117
Total intensity 41
Total Intensity 117
Trace Measurement Mode 122
Volume 41, 118, 236
X 118
Y 118
Measurement buffer 242
Measurement precision and accuracy
247
Measurement toolbar 75
MeasurementBackspace
Macro call 191
MeasurementLeftClick
Macro call 192
MeasurementRightClick
Macro call 192
Measurements
Automatic 44
Example 137
Fill 43
Trace 42
Track 43
Types 41
MeasureObjects
Macro call 193
Median filter 89
Defined 261
MedianFilter
Macro call 193
Memory allocation example 242

Menu
Edit 67
File 67
Image 67
Macro 68
Measurements 68
Mode 68
View 67
Worksheet 68
Menu bar 3
Menu structure 7
Message box
Example 136
Minor axis 111, 112, 118
Defined 261
Minor Axis End Point measurement
111
Minor Axis Length measurement
112
Minor Axis Slope measurement 112
Mosaic
Macro call 194
Mosaic filter 89
Multiply images 29, 87

N
New command 69
New Macro command 97
NewImage
Macro call 194
NewWorksheet
Macro call 194
Normal View command 76
NOT operation 95
NotOverlay
Macro call 194
Number of objects 41, 1035, 166
Number of Objects
measurement 113
Number of Pixels measurement 113

O
Object
Classify 100
Count 103
Counting example 137
Dilation See also Overlay, Dilate
Dilation filter 96
Edge tracking example 136
Elimination via a macro call 174
Erosion See also Overlay, Erode

Erosion filter 95
Remove objects touching boundary
96
Oilify
Macro call 195
Oilify filter 89
Open command 69
OpenImage
Macro call 195
OpenImageEx
Macro call 196
OpenWorksheet
Macro call 196
Options
Command 128
Location 7
OR operation 94
OrOverlays
Macro call 197
Overlay 63
Annotation 44, 64
Defined 262
Delete boundary objects 40
Delete residues 40
Dilate 39
Erode 37
Filters 35, 36
Grid 33
Logical AND 34
Logical NOT 35
Logical OR 35
Logical XOR 35
Math
Example 137
Number of 64
Paste 33
Overlay command 77
Overlay Draw Mode command 123
Overlay Filters command 95
Overlay Grid Mode command 123
Overlay Math command 94
Overlays
Cut, Copy and Clear 32
General 31
Hiding 32
Selecting 32

P
Page Setup command 71
Palette
ChangeColorResolution macro call
156

Index

277

Defined 262
StandardizePalette macro call 223
Standardizing the color palette 18
When changing resolution 82
When pasting overlays 33
Paste
Macro call 197
Paste command 16, 73
PasteToAnnotations
Macro call 198
PasteToNewImage
Macro call 198
Pause Macro command 98
Pause Recording Macro command
98
PCX file 12
Perimeter 41, 114, 235
Maximal perimeter 111, 114
Perimeter measurement 113
Perimeter-Diagonal measurement
114
Perimeter-Horizontal measurement
114
Perimeter-Vertical measurement 114
Photo CD file 12
Pixel
Defined 263
PlotGridLines
Macro call 198
PlotLines
Macro call 199
PlotRegression
Macro call 199
PlotSymbols
Macro call 199
Plotting data See
PlotXLogarithmic
Macro call 200
PlotYLogarithmic
Macro call 200
PlotYVsRow
Macro call 200
Point measurements
Defined 263
Posterize
Macro call 201
Posterize filter 89
Precision
Definition 247
Prewitt filter 89
Print
Macro call 201
Print command 71
Print Preview command 71

278

Index

PrintGraph
Macro call 201
Properties command 97
Pseudo-clearfield See Clearfield
equalization
Example 136
Pseudocolor
Defined 263

R
Recording toolbar 75
Red 77, 81, 143, 196, 205
Add noise filter 88
Adding noise to an image 144
Color separation 84, 211
Custom LUT 26
Custom LUT values 149
Hex code 237
Intensity measurement 115
Setting background color 152
Setting weighting value 203
Spiral LUT 26
RedrawPlot
Macro call 202
RedWeighting
Macro call 203
RefreshColumnLabels
Macro call 203
Regression Line
Graphing 130, 199
Replace command 74
Resample
Macro call 203
Resample command 91
Resize
Macro call 204
Resize command 92
Resolution
Definition 247
Intensity 249
Spatial 248
Restore command 91
RestoreImage
Macro call 204
RGB (Red, Green, Blue)
Defined 263
Roberts filter
Defined 264
Rotate
Macro call 204
Rotate 180 Degrees command 92
Rotate 270 Degrees command 93

Rotate 90 Degrees command 92

Rotate Any Angle command 93
Run SigmaPlot command 127
Run SigmaStat command 127

S
SamplingFrequency
Macro call 205
Saturation 2930, 41, 65, 109
Adjustment command 148
Color separation 211
Color thresholding 161
Command 84
Value measurement 115
Save
Macro call 205
Save All command 70
Save As command 70
Save command 69
SaveAll
Macro call 206
SaveAs
Macro call 206
SaveHistogram
Macro call 210
Screen resolution 6
Search For Help On command 132
Select
Macro call 210
Select Source command 71
SelectAllAnnotations
Macro call 210
Selection Mode command 121
Selection modes
Confusion between general
selection and annotation
selection modes 243
SelectTwainSource
Macro call 71, 211
Separate
Macro call 211
Separate command 84
SetAreaUnits
Macro call 212
SetCellText
Macro call 212
SetCellValue
Macro call 212
SetCursorWidth
Macro call 213
SetDistanceUnits
Macro call 213

SetGraphXColumn
Macro call 214
SetGraphYColumn
Macro call 214
SetIntensityUnits
Macro call 215
SetOverlayPixel
Macro call 216
SetPixelIntensity
Macro call 216
SetRoundCursor
Macro call 216
SetSquareCursor
Macro call 217
Settings
Location 7
Settings command 104
SetXAxisLabel
Macro call 217
SetYAxisLabel
Macro call 217
SetZoomLevel
Macro call 218
Shape factor 41, 13738
Defined 264
Shape Factor
Measurement 115
Sharpen
Macro call 218
Sharpen filter 89
Shift and Difference 89
Shift Columns Left command 104
Shift Columns Right command 104
ShiftColumnAssignmentsLeft
Macro call 218
ShiftColumnAssignmentsRight
Macro call 219
Show
Macro call 219
Show All Overlays command 78
ShowAllOverlays
Macro call 220
SigmaPlot, being run from
SigmaScanPro 127
SigmaStat, being run from
SigmaScan Pro 127
Slope 41, 112, 115, 116, 235
Slope measurement 115
Snap Window to Image command 96
Sobel filter 90
Sort Selection command 99
SourceOverlay
Macro call 220

Index

279

Spatial calibration See Calibrate

distance and area
Spatial resolution 248
SpatialFilter
Macro call 221
Splice
Macro call 222
Splice command 93
Splice images
Example 139
Standard toolbar 3, 75
Standardize Palette command 83
StandardizePalette
Macro call 223
Start Recording Macro command 98
Start/Resume Macro command 97
Statistics 49, 5760, 66
Print 60
Status Bar 3, 4
Status Bar command 75
Stop Recording Macro command 98
StretchHistogram
Macro call 223
Subtract images 29, 30, 87
Sum of X measurement 115
Sum of X Squared measurement 116
Sum of XY measurement 116
Sum of Y measurement 117
Sum of Y Squared measurement 117
Support
Technical 8
System requirements 6

T
Tally 41
Defined 265
Edge tracking macro 224
Line tracking macro 228
Measurement 117
While tracking edges 101
While tracking lines 102
Technical support 8
TGA file 12
Threshold
Color 68, 86, 161
Flood fill 43, 119, 122, 176, 177
Intensity 68, 86, 137, 187
Example 135, 136, 137, 138
TIF file 12
Defined 265
Tile command 131
Tip of the Day command 133

280

Index

Title bar 3
Toolbars command 74
Total intensity 41, 117
Total Intensity measurement 117
Trace Measurement Mode command
122
Track Edges command 101
Track Lines command 102
TrackEdge
Macro call 224
Tracking 243
Command 101, 102
Continuous points 120
Edge tracking macro 224
Individual points 120
Line width 120
Sample every 121, 205
Sampling grid 121, 184
Stability 101, 103, 227
Tab 120
Tracking stability 121
Tracking edges command 101
Tracking speed 101, 102, 227
Tracking stability
Defined 265
Tracking Tracking speed 120
TrackingBlackOnWhite
Macro call 224
TrackingGridOriginX
Macro call 225
TrackingGridOriginY
Macro call 225
TrackingGridSpacingX
Macro call 226
TrackingGridSpacingY
Macro call 226
TrackingSpeed
Macro call 227
TrackingStability
Macro call 227
TrackLine
Macro call 228
True color (32-bit) mode 241
Tutorials See Macros, Examples
Twain
What is it? 244
TwainNative
Macro call 228
TwainShowStatus
Macro call 228
TwainUI
Macro call 229

U
UncalibrateIntensity
Macro call 229
UncalibrateXY
Macro call 229
Undo
Macro call 230
Undo command 72
UnselectAllAnnotations
Macro call 230
User toolbar 75

V
VB See Visual Basic
VCR
Capturing unstable images 242
ViewAnnotations
Macro call 231
ViewGraph
Macro call 231
ViewOverlay
Macro call 231
Visual Basic See separate help file
Constants 234
Visual basic programming language
4
Volume 41, 118, 236
Volume measurement 118

Organization 50
Paste 53
Print 59
Save 58
Viewing 50

X
X Position measurement 118
XOR operation 94
XorOverlays
Macro call 232
XYContinuous
Macro call 232

Y
Y Position measurement 118

Z
Zoom 15, 74, 76, 126, 218
Defined 266
Example 135
Zoom In command 76
Zoom Out command 76

W
Width
Macro call 232
Window Leveling command 85
Word
Working directly with 245
Word 97
Run from SigmaScan Pro 139
Worksheet
Cell borders 57
Cell formatting 50
Cell selection 52
Clearing 50
Cut, Copy and Clear 52
Defined 266
Delete cells 54
Fonts 55
Format 54
Insert cells 54
Number alignment 56
Open 59

Index

281