I.

Bakteri Aerob dan Fakultatif

Basil Gram Positif
Bacillus anthracis
Bacillus cereus
Corynebacterium diphteriae
Corynebacterium jeikeium
Erysipelothrix rhusiopathiae
Listeria monocytogenes
Gardnerella vaginais
Nocardia asteroids
Rhodococcus equ
Arcanobacterium haemolyticumi
Mycobacterium sp.

II. Bakteri Anaerob

Basil Gram Positif Penghasil Spora
Clostridium botulinum
Clostridium difficile
Clostridium perfringens
Clostridium tetani
Basil Gram Positif Bukan Penghasil Spora
Actinomyces israelii
Propionibacterium acnes
Bifidobacterium sp.
Eubacterium sp.
Lactobacillus sp.
Spora: struktur sel istimewa yang memungkinkan kelangsungan hidup dalam
kondisi ekstrim
Saprofit: organisme yang hidup mendapatkan bahan organik dari organisme yang
telah mati atau membusuk
Fastidious: having complicated nutritional requirements; especially growing only in
special artificial cultures

B. cereus

B. anthracis

-hemolysis

No hemolysis

Hemolysis

Motile

Non-Motile

Motility

B. anthrachis

Spore stain procedure:

1. Make a heat fixed smear of
Bacillus
2. Place the slide on the slide rack
3. Cover the smear with malachite
green stain
4. Apply heat for 3-5 min without
boiling and drying of the slide
5. Wash the slide gently in running water about 20 S
6. Counterstain with safranin for one minute
7. Gently rinse with water
8. Gently blot the slide dry, no rubbing, and let it air dry and examine with oil
immersion optics.
9. Observe red vegetative cells and sporangia, and green endospores and free
spores

Spore Stain
Bacillus spores are oval & central
By spore staining technique (Malachite green & safranin) , the spore
appears green while the vegetative cells appear red.

Katalase positif

Bacillus

Clostridium