USING COMPOUND

MICROSCOPE
Name: Nguyen Minh Tuan - Mike (sobinmike25@gmail.com)
Partners name: Tram,Lucas, Jacky
Date: October 15, 2016
Honor code: On my honor, I have neither received nor given any unauthorized aid on
this assignment.

INTRODUCTION
Compound microscope consists of two or more double convex lenses fixed in the two
extremities of a hollow cylinder. The lower lens (nearest to the object) is called the
objective; the upper lens (nearest to the eye of the observer), the eyepiece. Compound
microscope is used for seeing the magnified images of small organism. It was invented
in 1595 by Zacharias Jansen.
There are many types of microscope, and there are some significant:
Optical microscope: also called light microscope, uses a visible light to zoom images of small
samples
Electron microscope: a microscope with high magnification and resolution, employing
electron beams in place of light and using electron lenses.

MATERIALS
Compound microscope
Slide
Cover slide
Toothpicks
Onion
Yogurt
Distilled water
Methylene blue

PROCEDURE

ONION ROOT
Step 1: Cut a tiny root of the onion
Step 2: Place it on the slide
Step 3: Put the cover slide on the slide
Step 4: Start the experiment, adjust the magnification from the low to high
( from 4x, 10x to 100x).
Use the oil when we observe at 100x for observation more clearer.

ONION CELL
Step 1: Cut a small amount of onion
Step 2: Place it on the slide
Step 3: Put the cover slide on the slide
Step 4: Start the experiment, adjust the magnification from the low to high
( from 4x, 10x to 100x).
Use the oil when we observe at 100x for observation more clearer.

DILUTED YOGURT
Step 1: Mix the yogurt with the distilled water
Step 2: Use a toothpick and dip it into the diluted yogurt
Step 3: Spread it on the slide and wait for 3 minutes for dry out
Step 4: Put a slide and slightly burn it 4,5 times
Step 5: Put a few drops of methylene blue then covered the slide with the
cover slide
Step 6: Start the experiment, adjust the magnification from the low to high

( from 4x, 10x to 100x).

Use the oil when we observe at 100x for observation more clearer.

UNDILUTED YOGURT
Step 1: Use a toothpick dip into the undiluted yogurt
Step 2: Spread in on the slide and wait for 3 minutes to dry out
Step 3: Slightly burn the slide 4,5 times
Step 4: Put a few drops of methylene blue then covered the slide with the
cover slide
Step 5: Start the experiment, adjust the magnification from the low to high
( 4x, 10x, 100x)
Use the oil when we observe at 100x for observation more clearer.

CHEEK CELL
Step 1: Use a toothpick and gently rub the inside of the cheek
Step 2: Spread it on the slide and wait for 3 minutes for dry out
Step 3: Put a slide and slightly burn it 4,5 times
Step 4: Put a few drops of methylene blue then covered the slide with the
cover slide
Step 5: Start the experiment, adjust the magnification from the low to high
( from 4x, 10x to 100x).
Use the oil when we observe at 100x for observation more clearer.

DATA & RESULT

ONION CELL

4x: Although we didnt focus on the cell pretty well, however, we can still the
shape of the onion cell which was slightly look like a square or an oval.
10x: After we had left it for about 5 hours (not enough time so we do it after
school) then you could probably see a huge different about the onion cell. The
color is now turning into blue and purple because of a pigment.

ONION ROOT CELL

4x: I can see the colour of the root is green, just as like the leaves. yet we
couldnt really focus into the image. The green roots look like a feather shape.
10x: We have magnified the image. And can see the pattern slightly wizen and
look like its been desiccated.
100x: The image is pretty blurry. It was one part because that the lens was touch
the slide too much so we couldnt really zoom much more in.

YOGURT DILUTE

4x: There was a pigment red orange color because of the affections of light. And
the diluted yogurts looks pretty clear because of the color blue and purple.
10x: The light is so bright for us to see the object clearly, however we can see
some dots at near the corner.
100x: There are some tiny dots that I think it could be bacteria.

YOGURT UNDILUTE

4x: The picture is unclear however we can still see the color blue.
10x: Now the blue dots are more vivid. It sparse everywhere.
100x: By now it is completely blurry and there might be a few dots.

CHEEK CELL

4x: At first you could see that there are many blue dots. And the color are very
pigmented.
10x: The shape of the dark blue cell look like torn up paper and it sparse
everywhere.

DISCUSSION
1+2

Onion cell have the brick like shape, its include cell wall and nucleus.
Cite: http://blogs.yis.ac.jp/18howet/2011/12/09/looking-at-cells/

Onion root cell have the rectangular cell, include cell wall and nucleus
Cite: https://www.biologycorner.com/worksheets/mitosis_onion_makeup.html

Yogurt bacteria
Bacteria most commonly found in yogurt is Lactobacillus bulgaricus and Streptococcus
thermophilus.
Cite: https://www.youtube.com/watch?v=AWrkWdg5ERU

3
There are many different between the pictures on the internet and from our groups. Our
groups picture are not as good and clearly as those on internet. Therefore its hard for
us and my individual to describe in details our data.

4
After doing experiments, I have learnt many skill, but generally, i have learnt how to
prepare for myself, how to work in the lab and how to use a compound microscope. I
think i will read the prelab more carefully the day before the experiment day to get ready
for the experiments and finish it on time.

RESEARCH QUESTION
Methylene blue is for dying to improve the visualization of the object. When we do a
experiments using methylene blue, we have to wear gloves.
Oil immersion is used for increasing the power of a microscope, especially when we
observe at 100x. First, we put a few drops of oil onto the slide that already placed the
organism. Then, we adjust the size of the magnification into 100x. Then we start to
observe the sample.