Lampiran 1.

Perhitungan Pengenceran
Rumus

: V1 x ppm1 = V2 x ppm2

Keterangan

: ppm1 = 10000 ppm

ppm2 = Seri konsentrasi
V2

= 50 mL

(1) Konsentrasi 1000 ppm

V1 X 10000 ppm = 50 mL x 1000 ppm
500 00
V1
= 10000
V1
= 5 mL
(2) Konsentrasi 750 ppm
V1 X 10000 ppm = 50 mL x 750 ppm
37500
10000

V1

V1

= 3,75 mL

(3) Konsentrasi 500 ppm

V1 X 10000 ppm = 50 mL x 500 ppm
2500 0
10000

V1

V1

= 2,5 mL

(4) Konsentrasi 250 ppm

V1 X 10000 ppm = 50 mL x 250 ppm
12500
10000

V1

V1

= 1,25 mL

(5) Konsentrasi 100 ppm

V1 X 10000 ppm = 50 mL x 100 ppm
500 0
10000

V1

V1

= 0,5 mL

(6) Konsentrasi 50 ppm

V1 X 10000 ppm = 50 mL x 50 ppm
2500
10000

V1

V1

= 0,25 mL

Lampiran 2. Tabel Pengamatan

Data Hasil Uji Toksisitas Ekstrak Daun Bayam Terhadap Larva Udang
Perlakua
n
1000 ppm
750 ppm
500 ppm
250 ppm
100 ppm
50 ppm
Kontrol

I
7
7
7
8
9
10
10

Hidup
Replikasi
II
6
8
9
10
10
10
10

III
8
8
8
7
9
10
10

Mati
Replikasi
II
4
2
1
0
0
0
0

I
3
3
3
2
1
0
0

III
2
2
2
3
1
0
0

Data Persen Kematian Uji Toksistas Ekstrak Daun Bayam Terhadap Kematian
Larva Udang.
Larva Udang
Perbandingan
%
Perlakuan
Total
Hidup
Mati
angka kematian Kematian
1000 ppm
750 ppm
500 ppm
250 ppm
100 ppm
50 ppm
Kontrol

21
23
24
25
28
30
30

9
7
6
5
2
0
0

30
30
30
30
30
30
30

9/30
7/30
6/30
5/30
2/30
0/30
0/30

30%
23,3%
20%
16,7%
6,7%
0%
0%

Lampiran 3. Perhitungan Persentase Kematian Larva Udang

Rumus :
% Kematian =

Kematian test kematian kontrol

Jumlah hewanuji(30)

(1) Konsentrasi 1000 ppm

% Kematian =

90
30

x 100% = 30%

(2) Konsentrasi 750 ppm

% Kematian =

70
30

x 100% = 23,3%

(3) Konsentrasi 500 ppm

% Kematian =

60
30

x 100% = 20%

(4) Konsentrasi 250 ppm

% Kematian =

50
30

x 100% = 16,7%

(5) Konsentrasi 100 ppm

% Kematian =

20
30

(6) Konsentrasi 50 ppm

00
% Kematian = 30
(7) Kontrol

x 100% = 6,7%

x 100% = 0%

x 100%

% Kematian =

00
30

x 100% = 0%

Lampiran 4. Perhitungan Nilai LC50 dengan Analisis Probit

Tabel Perhitungan Nilai LC50 Cara Probit
Hasil Uji
Konsentrasi
Log
%
Probit
(ppm)
konsentrasi kematian
(Y)
(X)
1000
3
30%
4,4756
750
2,87
23,3%
4,2710
500
2,69
20%
4,1684
250
2,39
16,7%
4, 0339
100
2
6,7%
3,5015
50
1,69
0%
0

14,64
20,4504

Hasil Perhitungan
X2

Y2

XY

9
8,2369
7,2361
5,7121
4
2,8561
37, 0412

20,0309
18,2414
17,3755
16,2723
12,2605
0
84,1806

13,4268
12,2577
11,2130
9,6410
7, 0030
0
53,5415

(X ) ( Y )n ( XY )
nilai slope (m) =
( ( X ))n (X )
=

(14,64 X 2 0 , 45 0 4)6(53,5415)
( 14,64)6 (37,0 412)

(299,39386)(321,249)
(214,3296)(222,2472)

21,85514
7,9176

= 2,76032
Intersep (b)

(X ) ( XY ) ( X ) ( Y )
( ( X ) ) n (X )

(14,64 X 53,5415)( 37,0 412 X 2 0 , 45 0 4 )

(14,64) 6(37, 0 412)

(783,84756)( 757,50736)
(214,3296)(222,2472)

26,34 02
7,9176

3,32679

Persamaan garis lurus :

Y

= mX + b

= 2,76032X

2,76032X

= 5 + 3,32679

3,32679

8,32679
2,76 0 32

= 3,0166

Sehingga nilai LC50 = Anti log 3,0166 = 1038, 962 g/mL

GRAFIK HUBUNGAN ANTARA LOG KONSENTRASI DAN PROBIT
5
4

f(x) = 2.76x - 3.33

R = 0.69

3
LOG KONSENTRASI

Y-Value
2

Linear (Y-Value)

1
0
1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2
PROBIT

Lampiran 6. Dokumentasi Penelitian

(1) Gambar tanaman bayam (Amaranthus tricolor L.)

(2) Proses maserasi daun bayam

(3) Proses penimbangan ekstrak daun bayam

(4) Larutan stok

(5) Larutan uji

(6) Proses pengujian ekstrak daun bayam terhadap larva udang

(7) Gambar larva udang

Wadah
Larva udang
Air laut

YAYASAN BINA HUSADA KENDARI

AKADEMI FARMASI BINA HUSADA KENDARI
PROGRAM STUDI D-III FARMASI
Jalan Asrama Haji No. 1/Kendari Sulawesi Tenggara 93117/Tlp./Fax. 0401-3190193

Nama

: Rista Puspita R.

NIM

: F.09.065

Judul KTI

: Uji Toksisitas Ekstrak Daun Bayam (Amaranthus tricolor

L.) Dengan Metode Brine Shrimp Lethality Test (BSLT).

Waktu Penelitian

: 29 Juni - 7 Juli 2013

Data Hasil Penelitian

Perlakuan
1000 ppm
750 ppm
500 ppm
250 ppm
100 ppm
50 ppm
Kontrol

I
7
7
7
8
9
10
10

Hidup
Replikasi
II
6
8
9
10
10
10
10

III
8
8
8
7
9
10
10

Mati
Replikasi
II
4
2
1
0
0
0
0

I
3
3
3
2
1
0
0

III
2
2
2
3
1
0
0

Kendari, 7 Juli 2013

Laboran Laboratorium Farmakologi

Asrawal, AMF
Mengetahui :
Kepala Laboratorium Farmakologi

Muh. Ilyas Yusuf, S.Farm, Apt