Mass Spectrometry

Mass spectrometry is one of the best ways to provide molecular structure,

identifying the unknown and probing the fundamental of principle. Mass
spectrometry involve in ions and been measured of by mass to charge ratio (m/z)
value. First, the molecule was introduces into the inlet where in inlet it introduce
the sample into vacuum of mass analyser. Then, it will be ionized and been
separated into fragments ions. Cation was the only charged that been detected
in mass spectrometry. Vacuum must be in mass spectroscopy thus gives low
pressure in order to minimize the ion-molecules reaction, scattering and
neutralization of ions. A variety of ionisation was occur in the mass spectrometry
which were ion impact ionization, chemical and field ionization which applicable
for gas phase ionization. Meanwhile for ionised condensed sample, fast atom
bombardment and electronspray was used. Then the product of the ions was
cation which then been accelerated or deflected by magnetic field or electric
field. The ions was been fragmented into several fragmentation. The molecular
ion was the ion obtained by the loss of electron from molecule. The fragments
ion will go to detector ad the peaks of (m/z) ratio result produced. Base peak was
the peak that has 100% intensity which indicate the highest peak in mass
spectra. Each peak represents the mass of its molecular ion. Basically, it gives us
information about the molecular weight of ions in unknown structure.
Advantages of this spectroscopy were high sensitivity and high accuracy. It also
can coupled
to Gas and
Liquid

Chromatography to identified the unknown compound by its molecular weight.